##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921460_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6098260
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.521399218793558	33.0	31.0	34.0	30.0	34.0
2	31.64760981001138	33.0	31.0	34.0	29.0	34.0
3	31.776693843817746	34.0	31.0	34.0	29.0	34.0
4	35.3742315021006	37.0	35.0	37.0	33.0	37.0
5	35.10841387543332	37.0	35.0	37.0	32.0	37.0
6	35.13915887482659	37.0	35.0	37.0	32.0	37.0
7	35.05074250687901	37.0	35.0	37.0	32.0	37.0
8	34.993347282667514	37.0	35.0	37.0	32.0	37.0
9	36.54762292850748	39.0	37.0	39.0	32.0	39.0
10-11	36.42945249956545	39.0	37.0	39.0	32.0	39.0
12-13	36.28643908262357	39.0	37.0	39.0	32.0	39.0
14-15	37.464862272189116	40.0	38.0	41.0	32.0	41.0
16-17	37.33870612273009	40.0	37.5	41.0	32.0	41.0
18-19	37.21070772646624	40.0	37.0	41.0	31.5	41.0
20-21	37.076446642156945	40.0	37.0	41.0	31.0	41.0
22-23	37.026327263842475	40.0	37.0	41.0	31.0	41.0
24-25	37.06889170025548	40.0	37.0	41.0	31.0	41.0
26-27	36.93210071397415	40.0	37.0	41.0	31.0	41.0
28-29	36.770210109113094	40.0	37.0	41.0	30.0	41.0
30-31	36.604791120745915	39.5	36.0	41.0	30.0	41.0
32-33	36.41006688793197	39.0	36.0	41.0	30.0	41.0
34-35	36.229003437045975	39.0	36.0	40.0	30.0	41.0
36-37	36.098346249585944	39.0	35.5	40.0	29.5	41.0
38-39	36.0369370935316	39.0	35.0	40.0	29.0	41.0
40-41	35.84251581598686	39.0	35.0	40.0	28.5	41.0
42-43	35.55507612007359	39.0	35.0	40.0	27.5	41.0
44-45	35.30678398428404	38.0	35.0	40.0	27.0	41.0
46-47	34.987019740057	38.0	34.0	40.0	26.0	41.0
48-49	34.59393023583776	38.0	34.0	40.0	24.5	41.0
50-51	34.26315760561209	38.0	33.0	40.0	24.0	41.0
52-53	33.90100405689492	38.0	33.0	40.0	23.0	41.0
54-55	33.52121113235579	37.0	33.0	40.0	22.0	41.0
56-57	33.07555753608406	37.0	32.0	39.0	20.5	40.5
58-59	32.62559287403292	36.0	31.5	39.0	18.5	40.0
60-61	32.12984925208174	35.5	31.0	39.0	17.0	40.0
62-63	31.64288026420651	35.0	30.5	38.5	13.5	40.0
64-65	31.060006050906324	35.0	30.0	38.0	8.0	40.0
66-67	30.466962871376424	34.0	29.0	37.5	7.5	39.5
68-69	29.907046928140158	34.0	28.5	37.0	6.5	39.0
70-71	29.28681017208187	34.0	28.0	36.0	2.0	39.0
72-73	28.67343865299282	33.0	26.5	35.5	2.0	38.0
74-75	28.102660185036388	33.0	26.0	35.0	2.0	37.5
76-77	27.03123661503445	31.5	25.5	34.0	2.0	36.0
78-79	27.976022914733058	33.0	26.5	35.0	2.0	36.0
80-81	27.97166708208571	33.0	27.0	35.0	2.0	36.0
82-83	27.56330658253338	33.0	26.0	35.0	2.0	36.0
84-85	26.97191821929534	32.5	25.5	35.0	2.0	35.0
86-87	26.353772059571092	32.0	24.5	34.0	2.0	35.0
88-89	25.711406204392727	31.5	23.5	34.0	2.0	35.0
90-91	25.09167811802055	31.0	20.0	34.0	2.0	35.0
92-93	24.512309576830113	31.0	18.5	34.0	2.0	35.0
94-95	23.84761653324063	31.0	11.0	34.0	2.0	35.0
96-97	22.876416715587723	30.0	2.0	34.0	2.0	35.0
98-99	21.64297037187657	29.5	2.0	34.0	2.0	35.0
100-101	18.166527747259053	23.5	2.0	31.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	63242.0
3	31131.0
4	17943.0
5	13359.0
6	11783.0
7	11770.0
8	13960.0
9	16261.0
10	19144.0
11	22658.0
12	26480.0
13	31071.0
14	34551.0
15	38781.0
16	43360.0
17	49839.0
18	50201.0
19	47150.0
20	48518.0
21	51733.0
22	55259.0
23	60559.0
24	68141.0
25	78625.0
26	93319.0
27	112447.0
28	135493.0
29	164579.0
30	199506.0
31	246128.0
32	311898.0
33	408552.0
34	552200.0
35	768802.0
36	1007482.0
37	919730.0
38	267893.0
39	4712.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.82560197851802	18.030209375393728	13.687693903418294	24.456494742669953
2	16.382164748633215	19.498889847267908	37.76500182019134	26.35394358390754
3	20.374841256513733	22.636460361357898	27.96499378145351	29.023704600674854
4	14.24849978227277	15.076109366996226	34.58678259534139	36.08860825538961
5	15.697818856411121	35.730211899433655	32.7274847115299	15.844484532625325
6	35.39979036299887	33.28517392520015	16.253611545393564	15.061424166407411
7	31.275309839118158	30.210739667202024	19.464803723566877	19.049146770112937
8	28.015207729868273	33.320841339863975	18.941818171073667	19.722132759194093
9	26.787541431122985	16.415095454484277	19.161047046585043	37.6363160678077
10-11	26.79744816534565	24.91708113106803	26.038929479527035	22.246541224059282
12-13	26.91480094066977	21.953756831694218	26.69063889138514	24.440803336250873
14-15	23.906485145269656	23.61249753765996	24.315498183590925	28.165519133479467
16-17	24.326867831706398	26.59041538629995	24.949473616004404	24.13324316598925
18-19	24.327134566852976	26.08788729030319	26.181695566757174	23.403282576086657
20-21	24.95658406231785	25.10570935060583	26.06927867336149	23.868427913714825
22-23	24.63385359458961	24.812978350577445	26.06914012160765	24.484027933225295
24-25	24.475926995046844	24.973038605174207	26.196904260207948	24.354130139570994
26-27	24.45039965156626	25.03875472496836	26.30057232259447	24.210273300870913
28-29	24.334975668411353	25.453971234451085	26.10628809473549	24.104765002402075
30-31	24.569319890912823	25.172856245967466	25.930974588059463	24.32684927506025
32-33	24.389126770139477	25.246499611630536	25.949861186512376	24.414512431717615
34-35	24.658381176655034	25.132325046449367	26.031539242593414	24.177754534302178
36-37	24.572813292119086	25.09830968674724	26.072812964119464	24.256064057014207
38-39	24.527322789969634	25.30005468846803	25.99368552794217	24.17893699362017
40-41	24.668346175672706	25.290797799580776	25.857705895667067	24.183150129079454
42-43	24.530233996650725	25.237387906532526	25.769698637397354	24.46267945941939
44-45	24.593854027305866	25.11746180651367	25.799202365923552	24.48948180025691
46-47	24.723439093944886	25.261532539141207	25.778057304946767	24.236971061967143
48-49	24.705787048292077	25.338616188359914	25.635085993708028	24.320510769639977
50-51	24.42169667347673	25.544537260583176	25.724141900785163	24.30962416515493
52-53	24.653905573177564	25.482078982059882	25.494951803990705	24.369063640771852
54-55	24.5798420097233	25.26019248664344	25.63474766086477	24.525217842768495
56-57	24.664277814802375	25.320864971872965	25.583162021683002	24.43169519164165
58-59	24.85627290331605	25.2452736377964	25.516288524347146	24.382164934540402
60-61	24.669479917562157	25.30800013611182	25.790098357187752	24.23242158913828
62-63	24.47681455580776	25.460584580867785	25.67452963249669	24.38807123082777
64-65	24.557847331988548	25.405996573719115	25.578991534004807	24.45716456028753
66-67	24.67458914303314	25.316728859623545	25.45570643447963	24.552975562863686
68-69	24.527257343977674	25.365265810040533	25.54895395793812	24.558522888043672
70-71	24.66707426333708	25.211843941424817	25.619016943087292	24.502064852150813
72-73	24.74303417659394	25.20035805113104	25.603255791675522	24.453351980599493
74-75	24.47367652545295	25.618075910805967	25.38472038516828	24.5235271785728
76-77	24.600529973327	25.256640044103047	25.499522556923942	24.64330742564601
78-79	24.562943575185226	25.260479656052315	25.643219914833566	24.533356853928897
80-81	24.631272755188434	25.32273604149551	25.55802728371237	24.487963919603686
82-83	24.70386685523134	25.189639046928264	25.615426808004877	24.491067289835517
84-85	24.56818985475775	25.36763234600444	25.51315177450877	24.551026024729037
86-87	24.542156323483407	25.408810617309264	25.536361417848756	24.512671641358573
88-89	24.479774162026747	25.266867693933637	25.48146288666837	24.771895257371245
90-91	24.601009593502663	25.204921801164197	25.491209732344757	24.702858872988383
92-93	24.756501083520757	25.348167891003833	25.54653856584146	24.34879245963395
94-95	24.69187876159972	25.20451286599429	25.4975403517527	24.60606802065329
96-97	24.468278755666926	25.1585262759137	25.71557187451059	24.65762309390879
98-99	24.535738449149	25.260251408194367	25.459058695668396	24.744951446988235
100-101	25.135660494289784	24.54207357161869	25.63727920372058	24.684986730370944
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	179.0
1	169.5
2	350.5
3	556.0
4	444.5
5	387.5
6	747.5
7	1731.0
8	2650.0
9	2494.5
10	1748.5
11	1341.0
12	1346.5
13	1472.5
14	1627.0
15	1714.0
16	1797.5
17	1977.0
18	2221.0
19	2398.0
20	2518.5
21	2718.5
22	3038.5
23	3407.0
24	3967.5
25	4931.5
26	6457.5
27	8557.5
28	11592.0
29	15857.0
30	21990.5
31	29326.5
32	37812.0
33	47412.0
34	57627.0
35	68952.5
36	82586.5
37	97066.5
38	110394.0
39	124593.0
40	140028.0
41	154894.0
42	169870.5
43	185667.0
44	201282.0
45	216458.5
46	231478.0
47	250820.0
48	275600.5
49	283677.5
50	273019.5
51	267402.0
52	266992.5
53	266089.5
54	263685.5
55	255179.0
56	240025.5
57	221788.0
58	203721.5
59	183952.5
60	161278.5
61	138429.0
62	113916.0
63	87872.5
64	65555.0
65	49663.5
66	38265.0
67	29809.0
68	22947.0
69	17603.0
70	13703.0
71	10491.0
72	7927.5
73	6204.0
74	4668.5
75	3494.0
76	2655.5
77	1723.5
78	1032.5
79	599.0
80	337.5
81	174.0
82	92.5
83	52.5
84	26.5
85	15.5
86	8.5
87	5.0
88	5.5
89	3.0
90	0.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3043163131778573
2	0.0
3	0.006100100684457534
4	0.05703266177565404
5	6.067304444218515E-4
6	0.0642150383879992
7	0.07433267850173655
8	0.1042592477198414
9	0.09212463883140437
10-11	0.09607658578020616
12-13	0.09498611079225877
14-15	0.06486276413271982
16-17	0.03587088776142703
18-19	0.037978046196784
20-21	0.028016188224181983
22-23	0.041134684319789575
24-25	0.03350955846421766
26-27	0.020686228530761235
28-29	0.023826468533647303
30-31	0.003304221204081164
32-33	0.00540318057937838
34-35	0.008059676038738918
36-37	0.011347499122700574
38-39	0.016340726699091215
40-41	0.02033366894819178
42-43	0.0036731789067701278
44-45	0.013979397401881848
46-47	0.023883861954065586
48-49	0.0027630832401373505
50-51	0.006920006690433009
52-53	0.0023121349368508395
54-55	0.0037141742070689017
56-57	0.0034764014653360137
58-59	0.0011642665284851744
60-61	0.005452374939736908
62-63	0.01475830810755855
64-65	0.006469058387146498
66-67	0.03792065277636572
68-69	0.06053366042116932
70-71	0.011954229567122426
72-73	0.03154998310993628
74-75	0.05007985884498201
76-77	0.2944118486256736
78-79	0.07067589771508594
80-81	0.03255846749728611
82-83	0.02751604556053694
84-85	0.01862826445576279
86-87	0.11415551321196538
88-89	0.5125314433953292
90-91	0.12934837150269093
92-93	0.046783836700960604
94-95	0.008830387684355866
96-97	0.006149295044816061
98-99	0.010601384657262892
100-101	0.00821545817987426
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	6098260.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.28046418776787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.26370739237	34.13347651661722
2	15.837395535936446	15.609484070724614
3	6.13273493015051	9.066720722950658
4	2.884091782841857	5.685175272742947
5	1.5690436316811631	3.86615992500544
6	1.007307483600309	2.9784348222981363
7	0.6627047718726652	2.2860879144133626
8	0.4416045219994596	1.7409980665240572
9	0.327053179886542	1.45055992670049
>10	1.7268718989873815	15.217932642869664
>50	0.10652121991795904	3.5917409559365994
>100	0.03943141708927787	3.1305972957279833
>500	8.99369174418394E-4	0.2942748275956561
>1k	5.662471769423605E-4	0.6584577118158111
>5k	3.330865745564921E-5	0.10966635020017682
>10k+	3.330865745564921E-5	0.18023297787725512
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	10980	0.1800513589122143	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6681	0.10955584051844296	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.199060059754749E-5
2	0.0	0.0	0.0	0.0	8.199060059754749E-5
3	0.0	0.0	0.0	0.0	8.199060059754749E-5
4	0.0	0.0	0.0	0.0	9.838872071705699E-5
5	1.63981201195095E-5	0.0	0.0	0.0	9.838872071705699E-5
6	1.63981201195095E-5	0.0	0.0	0.0	1.31184960956076E-4
7	1.63981201195095E-5	0.0	0.0	0.0	1.31184960956076E-4
8	1.63981201195095E-5	0.0	0.0	0.0	1.475830810755855E-4
9	1.63981201195095E-5	0.0	0.0	0.0	1.6398120119509498E-4
10-11	3.2796240239019E-5	0.0	0.0	0.0	1.6398120119509498E-4
12-13	4.9194360358528496E-5	8.19906005975475E-6	0.0	0.0	2.3777274173288773E-4
14-15	4.9194360358528496E-5	1.63981201195095E-5	0.0	1.63981201195095E-5	2.7876804203166145E-4
16-17	4.9194360358528496E-5	1.63981201195095E-5	0.0	4.0995300298773745E-5	3.197633423304352E-4
18-19	4.9194360358528496E-5	1.63981201195095E-5	0.0	5.7393420418283246E-5	3.443605225096995E-4
20-21	4.9194360358528496E-5	1.63981201195095E-5	0.0	6.5592480478038E-5	4.0175394292798274E-4
22-23	4.9194360358528496E-5	1.63981201195095E-5	0.0	9.838872071705699E-5	5.083417237047945E-4
24-25	4.9194360358528496E-5	1.63981201195095E-5	0.0	1.5578214113534027E-4	5.57536084063323E-4
26-27	4.9194360358528496E-5	1.63981201195095E-5	0.0	2.62369921912152E-4	5.739342041828324E-4
28-29	4.9194360358528496E-5	1.63981201195095E-5	0.0	6.641238648401347E-4	5.739342041828324E-4
30-31	6.5592480478038E-5	1.63981201195095E-5	0.0	0.0022547415164325562	5.739342041828324E-4
32-33	6.5592480478038E-5	1.63981201195095E-5	0.0	0.005796735462246608	5.90332324302342E-4
34-35	6.5592480478038E-5	1.63981201195095E-5	0.0	0.010757166798398232	6.067304444218515E-4
36-37	6.5592480478038E-5	4.099530029877375E-5	0.0	0.018136320852177507	6.067304444218515E-4
38-39	6.5592480478038E-5	6.5592480478038E-5	0.0	0.030049555119001155	6.067304444218515E-4
40-41	6.5592480478038E-5	6.5592480478038E-5	0.0	0.043004070013413664	6.067304444218515E-4
42-43	6.5592480478038E-5	8.199060059754749E-5	0.0	0.05628654731021636	6.149295044816062E-4
44-45	6.5592480478038E-5	8.199060059754749E-5	0.0	0.07036433343281526	6.395266846608704E-4
46-47	6.5592480478038E-5	8.199060059754749E-5	0.0	0.08536861334216646	6.969201050791537E-4
48-49	6.5592480478038E-5	9.018966065730223E-5	0.0	0.09965137596625923	7.379154053779275E-4
50-51	6.5592480478038E-5	9.838872071705699E-5	0.0	0.11439328595369827	7.54313525497437E-4
52-53	6.5592480478038E-5	1.1478684083656649E-4	0.0	0.129446760223408	7.953088257962107E-4
54-55	6.5592480478038E-5	1.5578214113534024E-4	0.0	0.1450085762168225	8.772994263937582E-4
56-57	6.5592480478038E-5	1.6398120119509498E-4	0.0	0.16261195816511595	9.428919068717961E-4
58-59	6.5592480478038E-5	1.6398120119509498E-4	0.0	0.17994477113143748	0.001008484387349834
60-61	6.5592480478038E-5	2.131755615536235E-4	0.0	0.19691682545512984	0.0010986740480071365
62-63	8.199060059754749E-5	2.131755615536235E-4	0.0	0.21352812113619296	0.0011642665284851744
64-65	8.199060059754749E-5	2.131755615536235E-4	0.0	0.230877332222634	0.0011642665284851744
66-67	9.838872071705699E-5	2.131755615536235E-4	0.0	0.2492678239366639	0.001180664648604684
68-69	9.838872071705699E-5	2.2137462161337824E-4	0.0	0.26933092390288377	0.0011970627687241935
70-71	9.838872071705699E-5	2.3777274173288773E-4	0.0	0.2889840708661159	0.0012544561891424766
72-73	1.475830810755855E-4	2.4597180179264247E-4	0.0	0.30795669584438845	0.001377442090038798
74-75	1.475830810755855E-4	2.4597180179264247E-4	0.0	0.3281591798316241	0.0014266364503973264
76-77	1.5578214113534024E-4	2.4597180179264247E-4	0.0	0.34890280178280364	0.0014266364503973264
78-79	1.6398120119509498E-4	2.4597180179264247E-4	0.0	0.3720651464516108	0.0014266364503973264
80-81	1.6398120119509498E-4	2.4597180179264247E-4	0.0	0.39653934072997876	0.0014594326906363455
82-83	1.8857838137435924E-4	2.4597180179264247E-4	0.0	0.4200378468612358	0.0015906176515924214
84-85	1.9677744143411398E-4	2.5417086185239724E-4	0.0	0.44288042818771256	0.001607015771711931
86-87	1.9677744143411398E-4	2.62369921912152E-4	0.0	0.46603457379645996	0.0016644091921302142
88-89	1.9677744143411398E-4	2.62369921912152E-4	0.0	0.4909597163781144	0.0017463997927277615
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	6715	0.0	26.526033	1
GTACATG	18800	0.0	24.927801	1
TACCTGG	7050	0.0	24.79246	2
TACATGG	19310	0.0	23.588333	2
ACATGGG	19925	0.0	22.289074	3
GAGTACA	14635	0.0	20.827349	1
AGTACAT	14005	0.0	19.737892	2
CATGGGG	14825	0.0	19.736416	4
GTATCAA	23990	0.0	19.137867	1
GTATAGG	1595	0.0	18.811638	1
ACCTGGG	9240	0.0	18.403051	3
AGAGTAC	17745	0.0	17.912907	10-11
GTGTAGC	3350	0.0	16.917994	1
TCAACGC	27025	0.0	16.64434	4
ATGGGGG	7205	0.0	16.60512	5
CAACGCA	27280	0.0	16.463503	5
TATCAAC	27910	0.0	16.337	2
ATCAACG	28015	0.0	16.089935	3
AACGCAG	28255	0.0	16.030605	6
CGTATAC	410	1.0713848E-8	15.060459	3
>>END_MODULE