FastQCFastQC Report
Mon 15 Aug 2016
SRR2921459_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921459_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5383041
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT90080.16734035650109297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64870.12050809198741008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG76250.039.480161
TACCTGG74950.038.4826932
ACCTGGG96100.028.8201313
GTACATG150350.025.1044941
TACATGG155450.023.628072
ACATGGG162400.021.346083
GTATAGG12650.020.3769191
CCTGGGG102350.019.3541564
GTATCAA204050.018.8360961
CCTATAC10550.018.2548923
AGTACAT110850.017.8704092
GAGTACA127950.017.8435971
CATGGGG128600.017.7568824
AGAGTAC160600.017.03423310-11
GTGTAGG17700.016.6436161
TCAACGC228450.016.579344
TATCAAC230500.016.5117682
GTGTAGC27050.016.5061381
CAACGCA229750.016.4056955
GTACCGG9950.016.191461
Produced by FastQC (version 0.11.5)