##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921459_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5383041
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.061314413172777	31.0	31.0	34.0	26.0	34.0
2	30.148334185082373	31.0	31.0	34.0	26.0	34.0
3	30.28212120249502	31.0	31.0	34.0	26.0	34.0
4	33.77667307382574	37.0	35.0	37.0	30.0	37.0
5	33.75154749889514	37.0	35.0	37.0	29.0	37.0
6	33.84744608112775	37.0	35.0	37.0	30.0	37.0
7	33.72594932864156	37.0	35.0	37.0	29.0	37.0
8	33.699441821082175	37.0	35.0	37.0	29.0	37.0
9	35.10948161085899	39.0	35.0	39.0	29.0	39.0
10-11	34.968579005807314	39.0	35.0	39.0	28.0	39.0
12-13	34.795110700438656	38.0	35.0	39.0	27.0	39.0
14-15	35.75542411807749	39.0	36.0	41.0	27.0	41.0
16-17	35.604584100325454	39.0	36.0	41.0	27.0	41.0
18-19	35.41660698850334	39.0	36.0	40.5	27.0	41.0
20-21	35.28975239088835	39.0	35.5	40.0	26.0	41.0
22-23	35.311117544897016	39.0	36.0	40.0	25.5	41.0
24-25	35.16432198825905	39.0	35.0	41.0	25.0	41.0
26-27	34.87482345016507	39.0	35.0	40.0	24.0	41.0
28-29	34.62465797678301	39.0	34.0	40.0	23.5	41.0
30-31	34.355281986520254	38.0	34.0	40.0	21.5	41.0
32-33	34.06279796122675	38.0	34.0	40.0	18.5	41.0
34-35	33.622602354319795	38.0	33.0	40.0	16.5	41.0
36-37	33.21987144441218	38.0	33.0	40.0	13.5	41.0
38-39	32.96872409108532	38.0	32.0	40.0	9.0	41.0
40-41	32.685900311738294	38.0	31.5	40.0	9.0	41.0
42-43	32.376312942814295	37.5	31.0	40.0	8.0	41.0
44-45	32.034304587314125	37.0	31.0	40.0	7.5	41.0
46-47	31.71523122339213	37.0	30.0	40.0	3.5	41.0
48-49	31.47380820617937	37.0	30.0	39.0	2.0	40.0
50-51	30.74788377053045	35.5	29.0	38.5	2.0	39.5
52-53	31.2948675293389	36.5	30.5	39.0	2.0	40.0
54-55	31.7130189979976	37.0	31.0	39.5	2.0	40.5
56-57	31.540339558996486	36.5	31.0	39.5	2.0	41.0
58-59	31.185718165624227	36.0	30.5	39.0	2.0	40.5
60-61	30.70196010024817	35.5	29.5	39.0	2.0	40.0
62-63	30.200370756975474	35.0	29.0	39.0	2.0	40.0
64-65	29.632737796349684	35.0	28.0	38.0	2.0	40.0
66-67	29.11137236368811	34.0	27.5	37.5	2.0	40.0
68-69	28.57019833213234	34.0	26.5	37.0	2.0	39.0
70-71	28.02228953857123	34.0	26.0	36.0	2.0	39.0
72-73	27.445945145132647	33.5	25.5	36.0	2.0	38.5
74-75	26.82500885651809	33.0	24.5	35.0	2.0	37.5
76-77	26.215285467823854	32.5	22.5	35.0	2.0	37.0
78-79	25.617750078440793	32.0	20.0	35.0	2.0	36.0
80-81	25.002097234630014	31.5	18.0	34.5	2.0	36.0
82-83	24.33645145931454	31.0	11.5	34.0	2.0	35.0
84-85	23.678075180924687	31.0	6.0	34.0	2.0	35.0
86-87	23.023877953000916	30.0	2.0	34.0	2.0	35.0
88-89	22.326090215549165	29.5	2.0	34.0	2.0	35.0
90-91	21.537750501993205	29.0	2.0	34.0	2.0	35.0
92-93	20.6773644302542	29.0	2.0	33.5	2.0	35.0
94-95	19.72847485278303	27.0	2.0	33.0	2.0	34.0
96-97	18.805034923568293	25.5	2.0	33.0	2.0	34.0
98	18.177493167895246	25.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	202574.0
3	34274.0
4	25739.0
5	21702.0
6	20335.0
7	21571.0
8	24428.0
9	28119.0
10	31252.0
11	34050.0
12	37443.0
13	41996.0
14	36328.0
15	36895.0
16	40520.0
17	46423.0
18	51667.0
19	51416.0
20	53699.0
21	58166.0
22	63428.0
23	70163.0
24	77708.0
25	86807.0
26	97431.0
27	110759.0
28	126549.0
29	147172.0
30	172225.0
31	206949.0
32	252777.0
33	320332.0
34	423410.0
35	574552.0
36	739962.0
37	720183.0
38	282568.0
39	11469.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.78283799522381	16.752947516945923	12.811460619934486	24.65275386789578
2	16.238203183558362	18.51907126049352	38.838486461411335	26.40423909453678
3	20.80601629422312	22.089056722728515	27.10845288148799	29.996474101560374
4	13.781758664630583	14.263761034774502	34.31547225191924	37.63900804867568
5	15.666402280696111	35.29913317710582	32.363036201794095	16.67142834040397
6	35.53125325881047	32.591543645893495	16.58939703664571	15.287806058650316
7	31.98537843130914	29.6406605835767	18.907218196192474	19.466742788921685
8	28.38121841681288	33.26873549451813	18.460644029924097	19.88940205874489
9	26.996697585196266	16.62482988615726	18.85356507522167	37.5249074534248
10-11	27.026514081785535	24.746250282408617	25.732292979619015	22.49494265618683
12-13	27.163369403966005	21.734140022424544	26.455872230257526	24.64661834335192
14-15	24.029386575715918	23.420069307400702	24.16971813014753	28.380825986735854
16-17	24.547778580925986	26.45482876577755	24.610995149556654	24.386397503739808
18-19	24.472181552929055	25.907457031138915	25.94253061782857	23.67783079810346
20-21	25.024330236397212	24.96728002691409	25.953847988754685	24.05454174793401
22-23	24.7212039174679	24.63907011970764	25.87620928628854	24.763516676535925
24-25	24.628080722249553	24.83705541524042	25.995604123502066	24.539259739007964
26-27	24.654150831456597	24.906699230446442	26.106441243802024	24.332708694294936
28-29	24.42069790952508	25.35787210802143	25.90756359157303	24.313866390880463
30-31	24.685574191293938	25.106936837888906	25.699459762883027	24.508029207934136
32-33	24.551132602732192	25.1458935183585	25.736556927832975	24.566416951076334
34-35	24.827438242527453	25.06738552014125	25.833549653254085	24.271626584077215
36-37	24.794909155265774	25.002586032657735	25.85259884835346	24.34990596372303
38-39	24.67143015116828	25.17938718267512	25.836013198084846	24.313169468071752
40-41	24.805656759536074	25.175417206282003	25.742936732130918	24.275989302051006
42-43	24.6721830053545	25.11800851595911	25.60938854427593	24.600419934410457
44-45	24.722182157315515	25.10244557746445	25.64863839220074	24.526733873019296
46-47	24.901571513996178	25.18145024659162	25.585516240583505	24.331461998828697
48-49	24.848477381557736	25.198330917782414	25.481476793910574	24.47171490674928
50-51	24.53642931222631	25.437442064749323	25.598582575877092	24.427546047147274
52-53	24.720136852604956	25.46265808588153	25.365386683253504	24.45181837826001
54-55	24.554029525116615	25.18604615324986	25.618672747335776	24.641251574297748
56-57	24.693159465588206	25.32633343882654	25.503664722044068	24.47684237354118
58-59	24.889052705600058	25.199902070876156	25.481844983946512	24.429200239577273
60-61	24.75577238953795	25.175616482651936	25.708580255603053	24.360030872207062
62-63	24.53097689488098	25.358476361332592	25.653314357744982	24.45723238604144
64-65	24.60812462322369	25.343954285594762	25.54853401799861	24.499387073182938
66-67	24.669840644803116	25.26918789148574	25.473023863304366	24.587947600406775
68-69	24.542944630918694	25.298788508140767	25.541942048515132	24.61632481242541
70-71	24.661142983300365	25.144917839922172	25.542528610787475	24.65141056598999
72-73	24.770084996830285	25.12505556910931	25.53684307750007	24.568016356560328
74-75	24.475470204245042	25.520127038210898	25.458954826401698	24.545447931142363
76-77	24.71924913183107	25.142502475951368	25.42582818427497	24.7124202079426
78-79	24.687866236342995	25.101050096350797	25.574954035487803	24.636129631818406
80-81	24.727439314820767	25.237271499490955	25.432383476190946	24.602905709497332
82-83	24.78778737573269	25.14894536530241	25.466046726523473	24.597220532441426
84-85	24.72675383632167	25.272555959687608	25.4216400216836	24.579050182307125
86-87	24.732285732514068	25.239657528701258	25.51612087231087	24.511935866473802
88-89	24.673369787813282	25.07585560891141	25.36988297682768	24.880891626447628
90-91	24.713393371364095	25.02856784651329	25.42139089452014	24.836647887602478
92-93	24.868097044457798	25.22828308305593	25.440687814109904	24.462932058376367
94-95	24.949230587678862	25.018431286029397	25.422142451008884	24.61019567528286
96-97	24.726620255278185	25.01823215706614	25.581138929130127	24.67400865852555
98	24.783072328625646	25.24664609074994	25.185082510122548	24.785199070501868
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	782.0
1	608.0
2	817.0
3	1181.0
4	884.0
5	695.5
6	1172.5
7	2283.0
8	3261.5
9	3180.5
10	2474.5
11	2082.5
12	2217.5
13	2569.5
14	2998.5
15	3477.0
16	3871.5
17	4081.5
18	4150.0
19	4103.5
20	3976.0
21	3808.0
22	3793.0
23	4050.0
24	4578.5
25	5476.0
26	6809.5
27	8901.5
28	11852.5
29	16049.0
30	21448.0
31	27695.5
32	35586.0
33	44120.0
34	52913.0
35	63105.0
36	74508.5
37	86960.5
38	100399.0
39	113571.5
40	127026.5
41	141716.5
42	156772.0
43	172549.0
44	188122.5
45	204279.0
46	226806.5
47	248661.5
48	255026.0
49	249523.5
50	245746.0
51	245063.5
52	245681.5
53	246595.5
54	242953.5
55	233876.5
56	220137.0
57	204771.5
58	187724.0
59	168436.0
60	148030.5
61	125114.0
62	101189.5
63	77572.5
64	57285.0
65	42908.0
66	32406.5
67	24568.0
68	18522.5
69	13858.0
70	10314.5
71	7761.5
72	6045.0
73	4644.5
74	3561.0
75	2662.0
76	1801.0
77	1113.0
78	669.0
79	412.5
80	256.5
81	146.5
82	92.0
83	62.5
84	46.5
85	32.0
86	19.5
87	17.0
88	15.0
89	16.0
90	14.5
91	8.5
92	5.0
93	8.0
94	9.5
95	10.5
96	10.0
97	6.0
98	3.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.45286669746710084
2	0.04566192232234531
3	0.052981205233250124
4	0.05158794071975301
5	0.06093210139027364
6	0.06304986345078925
7	0.041296360180054364
8	0.01902270482428055
9	0.01144334587085627
10-11	0.014285605478390374
12-13	0.010142965658258965
14-15	0.014629277391719661
16-17	0.013923356704881125
18-19	0.05431873916620735
20-21	0.08258343193001874
22-23	0.0971662671712885
24-25	0.06839071075252817
26-27	0.08300141128406788
28-29	0.08319646831595746
30-31	0.055656273099164585
32-33	0.031933622649353774
34-35	0.38292481888954594
36-37	0.5978126490212503
38-39	0.5487047191355221
40-41	0.544385599143681
42-43	0.5186473593643444
44-45	0.5043338885956842
46-47	0.4944045568294947
48-49	0.4845773977942951
50-51	0.49871438839124577
52-53	0.5759848383097955
54-55	0.5473393199122949
56-57	0.5061451324632303
58-59	0.4849582234279843
60-61	0.4972468164370288
62-63	0.5087830466087848
64-65	0.5276757134118057
66-67	0.5520485539679152
68-69	0.5084765284158155
70-71	0.45810537203784996
72-73	0.4407081424793161
74-75	0.422883645136643
76-77	0.4362775613263953
78-79	0.4309738677450162
80-81	0.4280480122666723
82-83	0.44638337326429434
84-85	0.4143940200344007
86-87	0.4175149325446341
88-89	0.4429838078513613
90-91	0.42715632297803413
92-93	0.42272574182511335
94-95	0.43225567109743357
96-97	0.4273792453001937
98	0.4222334550303444
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	5383041.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.46640198547209
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.41659340950379	40.53204091031466
2	13.892062863385837	15.13301360649243
3	4.974266596226747	8.12791212038976
4	2.205144953490313	4.804252458921542
5	1.2281676545912426	3.344693659026053
6	0.7258421170476168	2.3720405115060945
7	0.4982852419483704	1.8997863003971711
8	0.34246325483820517	1.492219304261668
9	0.24157516375015023	1.1841956980669734
>10	1.3479985011030373	13.221863682561077
>50	0.09010569135616603	3.3573516986261778
>100	0.03584911380000455	3.1839441762671767
>500	9.598413519143064E-4	0.35316369403047665
>1k	6.170378460396591E-4	0.7042147641636687
>5k	6.855976066986117E-5	0.2893074149749955
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	9008	0.16734035650109297	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6487	0.12050809198741008	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.3003802125973032E-4	0.0
5	0.0	7.43074407198459E-5	0.0	1.3003802125973032E-4	0.0
6	0.0	7.43074407198459E-5	0.0	1.486148814396918E-4	1.8576860179961476E-5
7	0.0	7.43074407198459E-5	0.0	1.8576860179961475E-4	1.8576860179961476E-5
8	0.0	7.43074407198459E-5	0.0	1.8576860179961475E-4	1.8576860179961476E-5
9	0.0	7.43074407198459E-5	0.0	2.4149918233949919E-4	1.8576860179961476E-5
10-11	0.0	7.43074407198459E-5	0.0	2.4149918233949919E-4	1.8576860179961476E-5
12-13	0.0	1.1146116107976885E-4	0.0	2.4149918233949919E-4	1.2074959116974959E-4
14-15	0.0	1.1146116107976885E-4	0.0	2.4149918233949919E-4	1.3932645134971108E-4
16-17	0.0	1.1146116107976885E-4	0.0	2.507876124294799E-4	1.8576860179961475E-4
18-19	0.0	1.1146116107976885E-4	0.0	2.6007604251946064E-4	2.229223221595377E-4
20-21	0.0	1.486148814396918E-4	0.0	2.786529026994221E-4	2.229223221595377E-4
22-23	0.0	1.486148814396918E-4	0.0	3.343834832393066E-4	2.693644726094414E-4
24-25	0.0	1.5790331152967254E-4	0.0	3.8082563368921026E-4	2.972297628793836E-4
26-27	0.0	1.6719174161965327E-4	0.0	4.644215044990369E-4	3.158066230593451E-4
28-29	0.0	1.6719174161965327E-4	0.0	8.359587080982664E-4	3.158066230593451E-4
30-31	0.0	1.6719174161965327E-4	0.0	0.002303530662315223	3.158066230593451E-4
32-33	3.715372035992295E-5	1.6719174161965327E-4	0.0	0.0051457902698493286	3.3438348323930653E-4
34-35	3.715372035992295E-5	1.6719174161965327E-4	0.0	0.008368875511072644	3.715372035992295E-4
36-37	3.715372035992295E-5	1.8576860179961475E-4	0.0	0.01333818560921234	3.8082563368921026E-4
38-39	3.715372035992295E-5	2.0434546197957624E-4	0.0	0.02106615944407631	3.9011406377919097E-4
40-41	3.715372035992295E-5	2.229223221595377E-4	0.0	0.029899456459647995	3.9011406377919097E-4
42-43	3.715372035992295E-5	2.229223221595377E-4	0.0	0.03888136835665937	3.994024938691717E-4
44-45	3.715372035992295E-5	2.229223221595377E-4	9.288430089980738E-6	0.04782612653331082	4.2726778413911394E-4
46-47	3.715372035992295E-5	2.229223221595377E-4	1.8576860179961476E-5	0.05807126492255957	4.458446443190754E-4
48-49	3.715372035992295E-5	2.507876124294799E-4	1.8576860179961476E-5	0.06839071075252817	4.458446443190754E-4
50-51	3.715372035992295E-5	2.6007604251946064E-4	1.8576860179961476E-5	0.07896094419492625	4.8299836467899837E-4
52-53	3.715372035992295E-5	2.8794133278940286E-4	1.8576860179961476E-5	0.08906675613282529	5.480173753088634E-4
54-55	3.715372035992295E-5	4.179793540491332E-4	1.8576860179961476E-5	0.10037077555233184	5.758826655788057E-4
56-57	3.715372035992295E-5	4.458446443190754E-4	1.8576860179961476E-5	0.11195344787453784	5.758826655788057E-4
58-59	3.715372035992295E-5	5.29440515128902E-4	1.8576860179961476E-5	0.12434421361457214	5.758826655788057E-4
60-61	3.715372035992295E-5	5.573058053988442E-4	1.8576860179961476E-5	0.13665138348379663	5.758826655788057E-4
62-63	3.715372035992295E-5	5.758826655788057E-4	1.8576860179961476E-5	0.14868918888041166	5.758826655788057E-4
64-65	3.715372035992295E-5	6.03747955848748E-4	2.7865290269942216E-5	0.16059695625576695	5.758826655788057E-4
66-67	3.715372035992295E-5	6.316132461186902E-4	3.715372035992295E-5	0.17359146995165	5.758826655788057E-4
68-69	3.715372035992295E-5	6.501901062986517E-4	3.715372035992295E-5	0.1876262878176109	5.758826655788057E-4
70-71	3.715372035992295E-5	7.244975470184975E-4	3.715372035992295E-5	0.20157750981276196	5.944595257587672E-4
72-73	3.715372035992295E-5	7.802281275583819E-4	3.715372035992295E-5	0.21397756398288625	6.501901062986517E-4
74-75	3.715372035992295E-5	7.802281275583819E-4	3.715372035992295E-5	0.22678630907696967	7.152091169285169E-4
76-77	4.644215044990369E-5	7.802281275583819E-4	3.715372035992295E-5	0.24070966578185082	7.43074407198459E-4
78-79	5.5730580539884425E-5	7.895165576483627E-4	3.715372035992295E-5	0.25628636304274854	7.523628372884398E-4
80-81	5.5730580539884425E-5	8.359587080982664E-4	3.715372035992295E-5	0.27198380989481596	7.80228127558382E-4
82-83	5.5730580539884425E-5	8.359587080982664E-4	3.715372035992295E-5	0.28705893193085474	7.988049877383434E-4
84-85	5.5730580539884425E-5	8.359587080982664E-4	3.715372035992295E-5	0.3024127068695929	7.988049877383434E-4
86	5.5730580539884425E-5	8.359587080982664E-4	3.715372035992295E-5	0.3138003221599092	7.988049877383434E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	7625	0.0	39.48016	1
TACCTGG	7495	0.0	38.482693	2
ACCTGGG	9610	0.0	28.820131	3
GTACATG	15035	0.0	25.104494	1
TACATGG	15545	0.0	23.62807	2
ACATGGG	16240	0.0	21.34608	3
GTATAGG	1265	0.0	20.376919	1
CCTGGGG	10235	0.0	19.354156	4
GTATCAA	20405	0.0	18.836096	1
CCTATAC	1055	0.0	18.254892	3
AGTACAT	11085	0.0	17.870409	2
GAGTACA	12795	0.0	17.843597	1
CATGGGG	12860	0.0	17.756882	4
AGAGTAC	16060	0.0	17.034233	10-11
GTGTAGG	1770	0.0	16.643616	1
TCAACGC	22845	0.0	16.57934	4
TATCAAC	23050	0.0	16.511768	2
GTGTAGC	2705	0.0	16.506138	1
CAACGCA	22975	0.0	16.405695	5
GTACCGG	995	0.0	16.19146	1
>>END_MODULE