FastQCFastQC Report
Mon 15 Aug 2016
SRR2921459_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921459_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5383041
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT92460.1717616492239238No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62850.11675556623105789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG81000.040.042651
TACCTGG84450.036.896022
ACCTGGG102200.029.2333833
GTACATG157100.023.0105111
TACATGG161650.021.7435932
CCTGGGG109500.020.6911014
ACATGGG167050.020.4438083
GAGTACA129650.019.837271
GTATACG3850.019.7933791
AGTACAT121400.018.7409742
GTATCAA212050.018.6872651
TATAACG4450.018.1453022
CATGGGG129350.018.1033864
GTATAGG13300.017.9051951
GTATATA15750.017.5391331
AGAGTAC165700.017.10548210-11
TAACGCA5150.016.6013974
TCAACGC235750.016.5614934
CAACGCA237750.016.4137765
CGTATAC3251.91656E-716.0763973
Produced by FastQC (version 0.11.5)