##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921459_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5383041
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89552689641413	33.0	31.0	34.0	30.0	34.0
2	32.00276980985283	34.0	31.0	34.0	30.0	34.0
3	32.13218011900708	34.0	31.0	34.0	30.0	34.0
4	35.67951219394391	37.0	35.0	37.0	35.0	37.0
5	35.4748644864492	37.0	35.0	37.0	33.0	37.0
6	35.51378876735288	37.0	35.0	37.0	33.0	37.0
7	35.443591085410645	37.0	35.0	37.0	33.0	37.0
8	35.39672668292885	37.0	35.0	37.0	33.0	37.0
9	37.01227131652908	39.0	37.0	39.0	33.0	39.0
10-11	36.916238795134575	39.0	37.0	39.0	33.0	39.0
12-13	36.793788399531046	39.0	37.0	39.0	33.0	39.0
14-15	38.074485871461874	40.0	38.0	41.0	33.0	41.0
16-17	37.97812518983229	40.0	38.0	41.0	33.0	41.0
18-19	37.87427069569041	40.0	38.0	41.0	33.0	41.0
20-21	37.751400741699726	40.0	38.0	41.0	32.5	41.0
22-23	37.7113777324007	40.0	38.0	41.0	32.5	41.0
24-25	37.76153172528316	40.0	38.0	41.0	33.0	41.0
26-27	37.64516153601654	40.0	38.0	41.0	32.0	41.0
28-29	37.50987982443381	40.0	38.0	41.0	32.0	41.0
30-31	37.368281237315486	40.0	37.0	41.0	32.0	41.0
32-33	37.196472217098105	40.0	37.0	41.0	31.0	41.0
34-35	37.03988321842617	40.0	37.0	41.0	31.0	41.0
36-37	36.93566777589099	40.0	37.0	41.0	31.0	41.0
38-39	36.89564105121994	40.0	37.0	41.0	31.0	41.0
40-41	36.72869220204713	40.0	36.0	41.0	30.0	41.0
42-43	36.48051788942347	39.0	36.0	41.0	30.0	41.0
44-45	36.26263212559592	39.0	35.5	41.0	30.0	41.0
46-47	35.97588937182533	39.0	35.0	40.0	29.0	41.0
48-49	35.62195457920532	39.0	35.0	40.0	28.0	41.0
50-51	35.33134960331901	38.5	35.0	40.0	27.0	41.0
52-53	35.00671005478131	38.0	34.0	40.0	26.5	41.0
54-55	34.66287048528889	38.0	34.0	40.0	26.0	41.0
56-57	34.25866011423655	37.5	33.5	40.0	25.5	41.0
58-59	33.8471726297459	37.0	33.0	40.0	24.0	41.0
60-61	33.39587762753432	36.5	32.5	39.0	23.0	40.5
62-63	32.936542281583954	36.0	32.0	39.0	22.0	40.0
64-65	32.40092087353598	35.0	31.0	38.5	20.5	40.0
66-67	31.845162427705827	35.0	31.0	38.0	18.5	40.0
68-69	31.32017775825969	35.0	30.5	37.0	17.5	39.5
70-71	30.743168684763873	34.0	30.0	36.5	12.5	39.0
72-73	30.17139066932613	34.0	29.0	36.0	8.0	39.0
74-75	29.635025536680846	34.0	29.0	35.5	7.0	38.0
76-77	28.405674877824634	32.0	27.0	34.5	6.5	36.0
78-79	29.33267757016898	34.0	29.0	35.0	6.5	37.0
80-81	29.344161042057827	34.0	29.0	35.0	2.0	36.5
82-83	28.98338652817246	34.0	29.0	35.0	2.0	36.0
84-85	28.459386896737364	33.5	28.5	35.0	2.0	35.5
86-87	27.911662106976337	33.0	27.0	35.0	2.0	35.0
88-89	27.33574460978469	33.0	26.0	35.0	2.0	35.0
90-91	26.814929981027454	32.5	25.0	34.0	2.0	35.0
92-93	26.336204572842746	32.0	25.0	34.0	2.0	35.0
94-95	25.785194744011797	32.0	23.5	34.0	2.0	35.0
96-97	24.951684187432345	31.5	19.0	34.0	2.0	35.0
98-99	23.87126960392834	31.0	4.5	34.0	2.0	35.0
100-101	20.3023930153978	25.5	2.0	32.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	37567.0
3	19685.0
4	11499.0
5	8569.0
6	7669.0
7	7693.0
8	9148.0
9	10935.0
10	12864.0
11	15484.0
12	18160.0
13	21657.0
14	24178.0
15	27307.0
16	30794.0
17	36702.0
18	36929.0
19	34103.0
20	34281.0
21	35632.0
22	38844.0
23	42641.0
24	48381.0
25	56164.0
26	67035.0
27	80474.0
28	97400.0
29	118062.0
30	144385.0
31	178963.0
32	227838.0
33	303862.0
34	423817.0
35	625105.0
36	937464.0
37	1094456.0
38	443886.0
39	13408.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.0132092888748	17.213646878012803	13.200642930319278	24.572500902793124
2	16.323988615357006	18.83951840604595	38.41586939426989	26.420623584327146
3	20.800057814733133	22.263799784160806	27.292789822823483	29.643352578282578
4	14.065465221879684	14.574249667012262	34.227719375072255	37.1325657360358
5	15.717177083846135	35.24448659347967	32.42339098070505	16.614945341969136
6	35.657234587187055	32.68730711271374	16.44043294860125	15.215025351497957
7	32.05054726508741	29.611096662460962	18.907224731990965	19.43113134046066
8	28.435135264299966	33.2646283655687	18.43469454585824	19.865541824273098
9	26.83034036719577	16.66932243952043	18.852516473384043	37.64782071989976
10-11	27.023753929090113	24.781858739349772	25.72686714350489	22.467520188055232
12-13	27.15092029194154	21.698450974615167	26.528002213464518	24.622626519978777
14-15	23.937962478024815	23.403234133834978	24.22846478324091	28.430338604899298
16-17	24.48370397178666	26.453796754127957	24.73238722074355	24.330112053341832
18-19	24.426314536536704	25.893001001107407	26.047274124096063	23.63341033825983
20-21	24.98786134224474	24.931883222860556	26.057847091748553	24.02240834314615
22-23	24.65648028597566	24.641055292561482	26.002431759503907	24.700032661958947
24-25	24.586148625890697	24.830989531334684	26.08032762651128	24.502534216263342
26-27	24.563711588382837	24.91362304213003	26.224369571304585	24.298295798182547
28-29	24.37225753165539	25.353010200147345	26.030452721541273	24.24427954665599
30-31	24.59915678834243	25.083786599773	25.845526871670632	24.471529740213935
32-33	24.44724536948663	25.119894670851522	25.879497739582895	24.553362220078952
34-35	24.71556111666913	25.082260202522377	25.99982536369404	24.20235331711445
36-37	24.671487647066037	25.047571308657812	25.975132867101685	24.305808177174466
38-39	24.58912498522901	25.17023796340957	25.96765766683042	24.272979384531006
40-41	24.66766524949412	25.165504280456908	25.85818866563588	24.308641804413096
42-43	24.552852959464403	25.153399250099717	25.739965954831728	24.55378183560416
44-45	24.573094760607926	25.026494042927784	25.809118038207156	24.59129315825713
46-47	24.71895591945233	25.205242402784044	25.769569588801676	24.30623208896194
48-49	24.727354264921463	25.251476852351544	25.607297997863977	24.413870884863016
50-51	24.41416262986032	25.438925765028564	25.708160753649896	24.438750851461226
52-53	24.651805306722686	25.476266731662513	25.443338433426693	24.42858952818811
54-55	24.526476553817893	25.17988652082786	25.601188443716865	24.69244848163738
56-57	24.662660992363918	25.325272940204325	25.562005653495984	24.450060413935777
58-59	24.889726467394073	25.204737972178226	25.502890779044613	24.402644781383092
60-61	24.765833354775253	25.25685482638688	25.675738564158134	24.301573254679735
62-63	24.524641534940567	25.35879020202317	25.68676617640119	24.429802086635082
64-65	24.560195121425597	25.381781018984988	25.56181150368881	24.496212355900603
66-67	24.636801798608246	25.306810502300713	25.458100692067596	24.598287007023444
68-69	24.499993958978678	25.349184973284743	25.586085947933828	24.564735119802748
70-71	24.655556855053753	25.151058924336912	25.58468361672664	24.608700603882696
72-73	24.74191688937798	25.156789879819513	25.534544317971918	24.566748912830587
74-75	24.448040359160697	25.580195924589376	25.420786607015327	24.550977109234598
76-77	24.61464913625701	25.19113607778354	25.50006884010112	24.69414594585833
78-79	24.55686142554651	25.169246699745123	25.687620488815107	24.58627138589326
80-81	24.624438127550828	25.353689984477835	25.49039392998431	24.531477957987022
82-83	24.662424692666548	25.23195643537422	25.56901145269802	24.536607419261212
84-85	24.600885477066864	25.38249917016137	25.489233186373163	24.5273821663986
86-87	24.57207169717593	25.38784453281276	25.55391715748091	24.486166612530408
88-89	24.506796276218175	25.23268034669976	25.469643697905475	24.790879679176587
90-91	24.579206247182565	25.212330194669224	25.467504954084447	24.740958604063767
92-93	24.718344759191822	25.424414584471112	25.527387166220755	24.329853490116314
94-95	24.72591940696169	25.221146004806428	25.474444302938743	24.57849028529314
96-97	24.484006227334877	25.214817044170978	25.687226903870165	24.613949824623983
98-99	24.54212774068914	25.38403043520508	25.429771678367345	24.644070145738436
100-101	25.049063052908583	24.67963291816644	25.563654985880934	24.707649043044043
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	341.0
1	292.0
2	624.0
3	996.5
4	692.5
5	483.5
6	894.0
7	1961.5
8	2816.0
9	2623.5
10	1817.0
11	1215.5
12	1118.0
13	1170.0
14	1269.5
15	1398.5
16	1521.5
17	1673.0
18	1876.0
19	2043.5
20	2191.5
21	2411.0
22	2671.5
23	3092.5
24	3711.5
25	4508.0
26	5725.5
27	7459.5
28	9855.0
29	13406.5
30	18258.0
31	23843.0
32	30746.0
33	38727.0
34	46763.0
35	55225.5
36	65990.0
37	78060.0
38	89808.0
39	102402.5
40	116756.5
41	129602.5
42	142548.5
43	158230.5
44	174942.0
45	191381.0
46	205062.5
47	223447.5
48	249689.0
49	257286.0
50	246527.5
51	242469.5
52	242758.0
53	242586.0
54	240223.0
55	232550.0
56	218990.5
57	201702.0
58	184936.5
59	167586.0
60	147221.0
61	125149.0
62	102060.0
63	78439.0
64	57660.0
65	42674.5
66	32111.5
67	24529.0
68	18535.5
69	13965.5
70	10662.0
71	8025.5
72	6002.5
73	4605.5
74	3545.0
75	2630.5
76	1814.0
77	1153.5
78	679.0
79	360.0
80	188.5
81	107.5
82	58.5
83	33.5
84	21.0
85	6.5
86	4.0
87	3.0
88	2.0
89	1.0
90	0.5
91	1.0
92	1.5
93	1.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3039731631247096
2	0.0
3	0.006130363859387287
4	0.05504323671322585
5	4.829983646789983E-4
6	0.06093210139027364
7	0.07129798937069214
8	0.10139250286222974
9	0.08954046606741431
10-11	0.09374812489817559
12-13	0.09374812489817559
14-15	0.06304057502069926
16-17	0.03526816905165686
18-19	0.037153720359922954
20-21	0.027382291905263215
22-23	0.04019103699934665
24-25	0.03328044501240098
26-27	0.020583161079397314
28-29	0.023332536386031614
30-31	0.00314877780050347
32-33	0.005489462183178616
34-35	0.007913742436663589
36-37	0.011053231807077077
38-39	0.01614329149638652
40-41	0.020072297424448375
42-43	0.003566757154552603
44-45	0.01298522526579307
46-47	0.02292384546207246
48-49	0.0026007604251946067
50-51	0.007105649018835264
52-53	0.0024707224039348763
54-55	0.003771102616532179
56-57	0.003288104251853181
58-59	0.001170342191337573
60-61	0.0052479630008391166
62-63	0.014619988961629683
64-65	0.0063439977514568435
66-67	0.036995817048393276
68-69	0.058628570727958414
70-71	0.011545518601846057
72-73	0.03037316639423701
74-75	0.04741743560935167
76-77	0.2884986385948017
78-79	0.07265410016382932
80-81	0.031654969746654354
82-83	0.026509179476805027
84-85	0.01802884280465261
86-87	0.11473997690153205
88-89	0.5121919004518078
90-91	0.12857973773560336
92-93	0.04611705539675436
94-95	0.00844318295179249
96-97	0.005963172117767633
98-99	0.01096034750617727
100-101	0.00792303086675357
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5383041.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.371213827538924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.52117003477602	34.32344551327618
2	16.276076395199716	16.07139295961528
3	5.994117706891332	8.878106010431077
4	2.7150136002029988	5.361740680011941
5	1.4225745916321688	3.511711717454783
6	0.9237935861185469	2.7365286403660667
7	0.6001753995789384	2.074197159064827
8	0.4261864101455742	1.6833072308550678
9	0.30923589481256053	1.3740616337347134
>10	1.6455535902822438	14.727604462065678
>50	0.11536054152326335	3.8810167733909795
>100	0.04889325338708817	3.9881318597755273
>500	0.001094304827777775	0.3851761037878367
>1k	6.792215596065947E-4	0.7142367878223232
>5k	7.546906217639436E-5	0.28934246834772276
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	9246	0.1717616492239238	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6285	0.11675556623105789	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.8576860179961476E-5	0.0
3	0.0	0.0	0.0	1.8576860179961476E-5	0.0
4	0.0	0.0	0.0	1.8576860179961476E-5	0.0
5	0.0	9.288430089980738E-5	0.0	1.8576860179961476E-5	0.0
6	0.0	9.288430089980738E-5	0.0	3.715372035992295E-5	0.0
7	0.0	9.288430089980738E-5	0.0	3.715372035992295E-5	0.0
8	0.0	1.1146116107976885E-4	0.0	3.715372035992295E-5	0.0
9	0.0	1.1146116107976885E-4	0.0	7.43074407198459E-5	0.0
10-11	0.0	1.1146116107976885E-4	0.0	1.021727309897881E-4	0.0
12-13	0.0	1.3932645134971108E-4	0.0	1.3003802125973032E-4	9.288430089980738E-5
14-15	0.0	1.486148814396918E-4	0.0	1.486148814396918E-4	9.288430089980738E-5
16-17	0.0	1.5790331152967254E-4	0.0	1.5790331152967254E-4	9.288430089980738E-5
18-19	0.0	1.6719174161965327E-4	0.0	1.6719174161965327E-4	1.486148814396918E-4
20-21	0.0	1.6719174161965327E-4	0.0	1.6719174161965327E-4	1.76480171709634E-4
22-23	0.0	1.6719174161965327E-4	0.0	2.229223221595377E-4	2.0434546197957624E-4
24-25	1.8576860179961476E-5	1.76480171709634E-4	9.288430089980738E-6	2.693644726094414E-4	2.0434546197957624E-4
26-27	1.8576860179961476E-5	1.8576860179961475E-4	1.8576860179961476E-5	3.52960343419268E-4	2.1363389206955697E-4
28-29	1.8576860179961476E-5	1.8576860179961475E-4	1.8576860179961476E-5	7.523628372884397E-4	2.229223221595377E-4
30-31	1.8576860179961476E-5	1.8576860179961475E-4	1.8576860179961476E-5	0.002238511651685358	2.3221075224951843E-4
32-33	1.8576860179961476E-5	1.8576860179961475E-4	1.8576860179961476E-5	0.005117924979579387	2.6007604251946064E-4
34-35	1.8576860179961476E-5	1.950570318895955E-4	1.8576860179961476E-5	0.008220260629632953	3.3438348323930653E-4
36-37	2.7865290269942216E-5	2.0434546197957624E-4	1.8576860179961476E-5	0.013161705437502705	3.52960343419268E-4
38-39	3.715372035992295E-5	2.0434546197957624E-4	1.8576860179961476E-5	0.020796794971466873	4.086909239591525E-4
40-41	3.715372035992295E-5	2.0434546197957624E-4	1.8576860179961476E-5	0.02961151512685859	4.086909239591525E-4
42-43	3.715372035992295E-5	2.0434546197957624E-4	1.8576860179961476E-5	0.03861200388404992	4.086909239591525E-4
44-45	3.715372035992295E-5	2.0434546197957624E-4	1.8576860179961476E-5	0.04741743560935167	4.179793540491332E-4
46-47	3.715372035992295E-5	2.1363389206955697E-4	1.8576860179961476E-5	0.05742107481626092	4.365562142290947E-4
48-49	3.715372035992295E-5	2.229223221595377E-4	1.8576860179961476E-5	0.06769407849577962	4.458446443190754E-4
50-51	3.715372035992295E-5	2.229223221595377E-4	1.8576860179961476E-5	0.07815285077709792	4.458446443190754E-4
52-53	3.715372035992295E-5	2.3221075224951843E-4	1.8576860179961476E-5	0.08807289411319735	4.458446443190754E-4
54-55	3.715372035992295E-5	3.158066230593451E-4	1.8576860179961476E-5	0.09911683749018446	4.458446443190754E-4
56-57	3.715372035992295E-5	3.715372035992295E-4	1.8576860179961476E-5	0.11040228004951105	4.458446443190754E-4
58-59	3.715372035992295E-5	4.086909239591525E-4	1.8576860179961476E-5	0.122486527596576	4.6442150449903686E-4
60-61	3.715372035992295E-5	4.458446443190754E-4	1.8576860179961476E-5	0.13445002555247118	4.737099345890176E-4
62-63	3.715372035992295E-5	4.458446443190754E-4	1.8576860179961476E-5	0.14603269787467715	5.201520850389213E-4
64-65	4.644215044990369E-5	4.737099345890176E-4	1.8576860179961476E-5	0.1574296015950835	5.387289452188828E-4
66-67	5.5730580539884425E-5	5.387289452188828E-4	1.8576860179961476E-5	0.16999684750682748	5.387289452188828E-4
68-69	5.5730580539884425E-5	5.387289452188828E-4	1.8576860179961476E-5	0.18344649427711957	5.387289452188828E-4
70-71	6.501901062986516E-5	5.573058053988443E-4	1.8576860179961476E-5	0.19677539145624193	5.387289452188828E-4
72-73	7.43074407198459E-5	5.944595257587672E-4	1.8576860179961476E-5	0.20871102412186715	6.130363859387286E-4
74-75	7.43074407198459E-5	6.130363859387286E-4	1.8576860179961476E-5	0.2210925014318115	6.501901062986517E-4
76-77	7.43074407198459E-5	6.130363859387286E-4	1.8576860179961476E-5	0.23471862837381324	6.501901062986517E-4
78-79	7.43074407198459E-5	6.130363859387286E-4	1.8576860179961476E-5	0.24974730825940208	6.594785363886323E-4
80-81	7.43074407198459E-5	6.316132461186902E-4	1.8576860179961476E-5	0.26505464104769033	6.687669664786131E-4
82-83	7.43074407198459E-5	6.316132461186902E-4	1.8576860179961476E-5	0.2794052655367105	7.059206868385361E-4
84-85	7.43074407198459E-5	6.316132461186902E-4	1.8576860179961476E-5	0.2943317726913096	7.059206868385361E-4
86-87	8.359587080982665E-5	6.316132461186902E-4	1.8576860179961476E-5	0.30971341292031773	7.337859771084784E-4
88-89	9.288430089980738E-5	6.316132461186902E-4	1.8576860179961476E-5	0.3273242763709212	7.616512673784205E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	8100	0.0	40.04265	1
TACCTGG	8445	0.0	36.89602	2
ACCTGGG	10220	0.0	29.233383	3
GTACATG	15710	0.0	23.010511	1
TACATGG	16165	0.0	21.743593	2
CCTGGGG	10950	0.0	20.691101	4
ACATGGG	16705	0.0	20.443808	3
GAGTACA	12965	0.0	19.83727	1
GTATACG	385	0.0	19.793379	1
AGTACAT	12140	0.0	18.740974	2
GTATCAA	21205	0.0	18.687265	1
TATAACG	445	0.0	18.145302	2
CATGGGG	12935	0.0	18.103386	4
GTATAGG	1330	0.0	17.905195	1
GTATATA	1575	0.0	17.539133	1
AGAGTAC	16570	0.0	17.105482	10-11
TAACGCA	515	0.0	16.601397	4
TCAACGC	23575	0.0	16.561493	4
CAACGCA	23775	0.0	16.413776	5
CGTATAC	325	1.91656E-7	16.076397	3
>>END_MODULE