FastQCFastQC Report
Mon 15 Aug 2016
SRR2921458_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921458_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2459273
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24930.10137142155425607No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG38800.043.0731471
TACCTGG37800.043.054372
ACCTGGG47650.032.9998473
TATAACG2101.8189894E-1228.37952
GTATCAA60800.026.8816851
GTACATG70400.023.3467941
TAACGCA2604.0017767E-1122.9219064
TCGCGTA1402.3558727E-522.9055929
TATCAAC72400.022.4153422
TACATGG73000.022.2939072
TCAACGC71450.022.0075954
CAACGCA72250.021.9542685
AACGCAG76500.021.26836
ATCAACG74400.021.2582193
ACATGGG72950.021.052333
CCTGGGG53600.020.9547254
ATAACGC2851.3460522E-1020.9112113
GTGTAGC12050.019.4856451
GTATAGG5400.018.756881
GAGTACA55150.018.699681
Produced by FastQC (version 0.11.5)