##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921458_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2459273
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.072155876960387	31.0	31.0	34.0	26.0	34.0
2	30.156680856497022	31.0	31.0	34.0	26.0	34.0
3	30.28748983947695	31.0	31.0	34.0	26.0	34.0
4	33.778944021261566	37.0	35.0	37.0	30.0	37.0
5	33.75339500738633	37.0	35.0	37.0	28.0	37.0
6	33.851728945912065	37.0	35.0	37.0	30.0	37.0
7	33.72803060091336	37.0	35.0	37.0	29.0	37.0
8	33.70110191101191	37.0	35.0	37.0	29.0	37.0
9	35.11981955642989	39.0	35.0	39.0	29.0	39.0
10-11	34.977524455398	39.0	35.0	39.0	28.0	39.0
12-13	34.80346447913672	38.5	35.0	39.0	27.0	39.0
14-15	35.77089225149058	39.5	36.0	41.0	27.0	41.0
16-17	35.61100922915024	39.0	36.0	41.0	27.0	41.0
18-19	35.423735388466426	39.0	36.0	40.5	27.0	41.0
20-21	35.29403831945457	39.0	35.5	40.0	26.0	41.0
22-23	35.33021628749634	39.0	36.0	41.0	25.5	41.0
24-25	35.182715583019856	39.0	35.0	41.0	25.0	41.0
26-27	34.89270142029779	39.0	35.0	40.0	24.0	41.0
28-29	34.64153064747184	39.0	34.0	40.0	23.5	41.0
30-31	34.377526407194324	38.0	34.0	40.0	21.5	41.0
32-33	34.08382761897521	38.0	34.0	40.0	18.5	41.0
34-35	33.64684725933233	38.0	33.0	40.0	16.5	41.0
36-37	33.24782486531589	38.0	33.0	40.0	13.5	41.0
38-39	33.00044403366361	38.0	32.0	40.0	9.0	41.0
40-41	32.70848824022384	38.0	31.5	40.0	9.0	41.0
42-43	32.40380246520008	37.5	31.0	40.0	8.0	41.0
44-45	32.068635324341784	37.0	31.0	40.0	7.5	41.0
46-47	31.754825104817563	37.0	30.0	40.0	3.5	41.0
48-49	31.521372779679197	37.0	30.0	40.0	2.0	41.0
50-51	30.7903223838915	36.0	29.0	38.5	2.0	39.5
52-53	31.328838847903427	36.5	30.5	39.0	2.0	40.0
54-55	31.74732207445046	37.0	31.0	39.5	2.0	40.5
56-57	31.580822259261172	36.5	31.0	40.0	2.0	41.0
58-59	31.228743819819925	36.0	30.5	39.0	2.0	41.0
60-61	30.75035122168218	35.5	29.5	39.0	2.0	40.0
62-63	30.249371663902302	35.0	29.0	39.0	2.0	40.0
64-65	29.68561034094222	35.0	28.0	38.0	2.0	40.0
66-67	29.178585094050153	34.0	28.0	37.5	2.0	40.0
68-69	28.637412153917033	34.0	26.5	37.0	2.0	39.0
70-71	28.09788075581686	34.0	26.0	36.0	2.0	39.0
72-73	27.520100655762903	33.5	25.5	36.0	2.0	39.0
74-75	26.907285201764914	33.0	24.5	35.0	2.0	37.5
76-77	26.300099460287655	32.5	22.5	35.0	2.0	37.0
78-79	25.712435341663976	32.0	20.5	35.0	2.0	36.5
80-81	25.106849666547795	31.5	18.5	34.5	2.0	36.0
82-83	24.45118801369348	31.0	13.5	34.0	2.0	35.5
84-85	23.803982518410926	31.0	7.0	34.0	2.0	35.0
86-87	23.155848496689877	30.0	2.0	34.0	2.0	35.0
88-89	22.461859256780357	30.0	2.0	34.0	2.0	35.0
90-91	21.690621171378694	29.0	2.0	34.0	2.0	35.0
92-93	20.84420456777267	29.0	2.0	34.0	2.0	35.0
94-95	19.910612811184443	27.0	2.0	33.0	2.0	34.5
96-97	18.996389176801436	25.5	2.0	33.0	2.0	34.0
98	18.372336052158502	25.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	91747.0
3	15658.0
4	12046.0
5	9889.0
6	9300.0
7	9884.0
8	11231.0
9	12938.0
10	14641.0
11	15826.0
12	17454.0
13	19456.0
14	16664.0
15	16747.0
16	18637.0
17	20898.0
18	23285.0
19	23256.0
20	24462.0
21	26269.0
22	28411.0
23	31557.0
24	35228.0
25	38868.0
26	44018.0
27	49651.0
28	56912.0
29	65664.0
30	77449.0
31	92334.0
32	114033.0
33	144100.0
34	190503.0
35	257458.0
36	337494.0
37	338344.0
38	140773.0
39	6187.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.73642524139531	16.577881342499463	12.775813243611134	24.909880172494088
2	16.263181019332162	18.498869035995575	38.74336880817549	26.494581136496777
3	20.623566861359247	22.370714417069586	26.907447520070043	30.098271201501127
4	13.873314702624018	14.342730463488055	34.036995885594045	37.74695894829389
5	15.772354547892153	35.178514537378256	32.28967420339106	16.759456711338533
6	35.62825912881574	32.40212848372796	16.665296828920557	15.304315558535745
7	31.98867648829573	29.60201737172165	18.980835131361847	19.428471008620775
8	28.464285583559768	33.330242390892785	18.526864966892344	19.678607058655107
9	27.14156392056199	16.73368648875507	18.582709699248547	37.54203989143439
10-11	26.988997814284765	24.737786357875336	25.70426738074696	22.568948447092943
12-13	27.06117622897903	21.768257657322433	26.424291963916264	24.74627414978228
14-15	24.00598232550195	23.314436071267348	23.94441032815353	28.73517127507717
16-17	24.59400408105583	26.464274494214436	24.43523535206986	24.506486072659875
18-19	24.640340711330104	25.72182578212075	25.9288507156366	23.708982790912547
20-21	25.018832433866717	24.956952983419725	25.775283788804888	24.24893079390867
22-23	24.730089488850577	24.71671836360221	25.718453760634063	24.83473838691315
24-25	24.519368296923723	24.954375236770648	25.891997182556427	24.634259283749202
26-27	24.656801420165735	24.98357872323454	25.90827770719093	24.451342149408795
28-29	24.59105361421573	25.315453449389786	25.695733348255846	24.397759588138637
30-31	24.76954388862839	25.107739439907483	25.65214782146123	24.4705688500029
32-33	24.501839134787044	25.16684001134028	25.661655331131715	24.669665522740964
34-35	24.71466506644786	25.15217386867184	25.737968252801913	24.39519281207839
36-37	24.726330117249397	25.227112148091237	25.562630585649664	24.483927149009705
38-39	24.75613580909753	25.095245552992594	25.708284655907676	24.440333982002198
40-41	24.7884115049187	25.118566836777045	25.644128984035845	24.44889267426841
42-43	24.690216216061543	25.015502985525508	25.62088588371923	24.673394914693723
44-45	24.78168232335948	25.06946192856886	25.5263697835093	24.62248596456236
46-47	24.934733317966852	25.175733708667842	25.460790131448814	24.428742841916495
48-49	24.80519215170269	25.215444056465568	25.352848511843344	24.626515279988396
50-51	24.61896668731268	25.37938830730172	25.364470867158968	24.637174138226637
52-53	24.687370195614378	25.51635941736378	25.27942646887691	24.516843918144932
54-55	24.657564774212332	25.088690907485038	25.442820738870086	24.810923579432544
56-57	24.677333818358687	25.267477517337785	25.46336100089614	24.591827663407393
58-59	24.829335479524854	25.25681662015291	25.410874922434505	24.50297297788773
60-61	24.876282716853403	25.048404891514803	25.462597502011292	24.6127148896205
62-63	24.635263654301863	25.203967664373277	25.564110765155917	24.596657916168937
64-65	24.670198207111255	25.160293983878105	25.513413959839376	24.656093849171267
66-67	24.648700128626448	25.312258033121466	25.38518929106605	24.653852547186034
68-69	24.700438640681895	25.24772274916918	25.416941630317645	24.634896979831275
70-71	24.709298644040516	25.054324579712205	25.512166704047367	24.72421007219991
72-73	24.787735564864494	25.101192332522327	25.391672890666534	24.719399211946648
74-75	24.501660400305227	25.336546796171692	25.390494746813204	24.771298056709874
76-77	24.724280548065163	25.120694908594537	25.417183687439802	24.73784085590049
78-79	24.73755877690934	25.01412116140273	25.49140212207463	24.7569179396133
80-81	24.672943189673042	25.20311573109309	25.309362610614272	24.814578468619597
82-83	24.768963082499283	25.20635390074384	25.26203110693271	24.762651909824168
84-85	24.809503352986	25.141228864672655	25.27502570581764	24.77424207652371
86-87	24.713619176137147	25.13970655345173	25.444244277845396	24.702429992565726
88-89	24.73321132261134	24.931524699001756	25.36101306070248	24.974250917684426
90-91	24.772967303861147	24.981417543309377	25.27009907104053	24.97551608178895
92-93	24.76018137983688	25.19341594540881	25.38253875006202	24.663863924692286
94-95	24.847354986982364	24.941701949775965	25.39287239328225	24.818070669959415
96-97	24.77646404893358	25.04182588547028	25.371001168653933	24.810708896942202
98	24.90677702731124	25.08732187990439	25.175450310552666	24.830450782231708
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	312.0
1	232.5
2	304.0
3	434.5
4	325.5
5	284.0
6	430.5
7	791.0
8	1165.5
9	1181.0
10	987.5
11	908.0
12	981.0
13	1131.5
14	1356.0
15	1524.5
16	1661.5
17	1741.0
18	1770.5
19	1826.5
20	1728.0
21	1613.0
22	1742.0
23	1901.0
24	2144.0
25	2565.5
26	3232.5
27	4172.5
28	5527.0
29	7342.0
30	9480.0
31	12416.5
32	15819.5
33	19376.0
34	23704.0
35	28998.5
36	34257.0
37	39836.0
38	46040.5
39	51941.0
40	57521.0
41	63688.0
42	71171.5
43	79455.5
44	87163.5
45	93563.0
46	100519.5
47	108606.5
48	113235.0
49	112786.5
50	111786.0
51	110891.0
52	110740.5
53	112293.0
54	112079.5
55	107898.0
56	101018.5
57	94710.0
58	87693.5
59	79555.0
60	70160.5
61	58720.0
62	47352.0
63	36212.0
64	26849.0
65	20293.0
66	15499.5
67	11733.5
68	8938.5
69	6860.0
70	5131.5
71	3846.0
72	2886.5
73	2142.0
74	1626.0
75	1209.0
76	813.5
77	517.0
78	325.0
79	192.0
80	111.5
81	71.5
82	40.5
83	31.5
84	21.5
85	10.0
86	8.5
87	7.5
88	7.0
89	6.0
90	5.0
91	6.5
92	6.5
93	5.0
94	3.5
95	3.5
96	2.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.46440553773411897
2	0.04851027112484055
3	0.056154806725402184
4	0.053837048591189346
5	0.06400265444300002
6	0.06347404293870587
7	0.04167898399242378
8	0.01935531354184753
9	0.01215806459876557
10-11	0.014333504251053054
12-13	0.010999185531659152
14-15	0.014760459696829103
16-17	0.014252179404238568
18-19	0.05515857735192474
20-21	0.08441519099343586
22-23	0.101107115802109
24-25	0.07018334280090092
26-27	0.08518777703817347
28-29	0.0868346051861668
30-31	0.05593116339666235
32-33	0.03171669025764931
34-35	0.3906845640967879
36-37	0.6097737014150116
38-39	0.5591896466964018
40-41	0.5532529328789443
42-43	0.5273916315919379
44-45	0.5127124967419233
46-47	0.50376676359233
48-49	0.4927472468489671
50-51	0.5071620759468347
52-53	0.5891375215358361
54-55	0.5571158631026324
56-57	0.5150099236644325
58-59	0.4939467883394808
60-61	0.5068367765595767
62-63	0.5182629175370119
64-65	0.5375775686554523
66-67	0.5619343602763907
68-69	0.517937618149754
70-71	0.4670485952555897
72-73	0.4508852819512108
74-75	0.43169261810299225
76-77	0.4452941987327149
78-79	0.4399674212663661
80-81	0.4363281343714179
82-83	0.45678133334526094
84-85	0.4233364900928039
86-87	0.42612186609619995
88-89	0.45247111646409327
90-91	0.43618581588949257
92-93	0.4310013569050691
94-95	0.4417362366845812
96-97	0.43628747194801065
98	0.42996446510818437
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2459273.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.07528444583512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.64573226772978	51.6739823439745
2	11.506172860591388	14.745225978506832
3	3.3893340561334675	6.515176311861243
4	1.4741388603813543	3.7782346716637805
5	0.8023879017991122	2.570661652183747
6	0.4928851957146067	1.8949055468732725
7	0.34021384329012677	1.5259509146857952
8	0.2291068184326167	1.1744067647640175
9	0.18222357792982133	1.0508424829731997
>10	0.8695407929896488	9.742158408241583
>50	0.04628437436580939	2.027196271441728
>100	0.020449027860682253	2.3920579259255956
>500	0.0010840494703466869	0.4496563904197044
>1k	4.4637331131540224E-4	0.4595443364848816
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2493	0.10137142155425607	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	4.066242340724271E-5	0.0	0.0	0.0	0.0
12-13	4.066242340724271E-5	0.0	0.0	0.0	4.066242340724271E-5
14-15	4.066242340724271E-5	0.0	0.0	0.0	8.132484681448542E-5
16-17	4.066242340724271E-5	0.0	0.0	2.0331211703621355E-5	8.132484681448542E-5
18-19	4.066242340724271E-5	2.0331211703621355E-5	0.0	4.066242340724271E-5	8.132484681448542E-5
20-21	4.066242340724271E-5	4.066242340724271E-5	0.0	4.066242340724271E-5	8.132484681448542E-5
22-23	4.066242340724271E-5	4.066242340724271E-5	0.0	8.132484681448542E-5	1.4231848192534948E-4
24-25	4.066242340724271E-5	4.066242340724271E-5	0.0	8.132484681448542E-5	1.6264969362897084E-4
26-27	4.066242340724271E-5	4.066242340724271E-5	0.0	1.4231848192534948E-4	1.6264969362897084E-4
28-29	4.066242340724271E-5	4.066242340724271E-5	0.0	3.8629302236880576E-4	1.6264969362897084E-4
30-31	4.066242340724271E-5	4.066242340724271E-5	0.0	0.0017281529948078153	1.6264969362897084E-4
32-33	4.066242340724271E-5	4.066242340724271E-5	0.0	0.004127235975835135	2.0331211703621355E-4
34-35	4.066242340724271E-5	4.066242340724271E-5	0.0	0.007624204388858008	2.0331211703621355E-4
36-37	4.066242340724271E-5	4.066242340724271E-5	0.0	0.01213773338706195	2.0331211703621355E-4
38-39	4.066242340724271E-5	4.066242340724271E-5	0.0	0.020473530185546707	2.0331211703621355E-4
40-41	4.066242340724271E-5	8.132484681448542E-5	0.0	0.029154957582993024	2.0331211703621355E-4
42-43	4.066242340724271E-5	8.132484681448542E-5	0.0	0.03757207922829226	2.0331211703621355E-4
44-45	4.066242340724271E-5	8.132484681448542E-5	0.0	0.046273837837442204	2.236433287398349E-4
46-47	4.066242340724271E-5	8.132484681448542E-5	0.0	0.0549349340231849	2.4397454044345626E-4
48-49	4.066242340724271E-5	8.132484681448542E-5	0.0	0.06371801747914932	2.4397454044345626E-4
50-51	4.066242340724271E-5	8.132484681448542E-5	0.0	0.07313136849792601	2.6430575214707764E-4
52-53	4.066242340724271E-5	8.132484681448542E-5	0.0	0.08374426100721635	2.8463696385069896E-4
54-55	4.066242340724271E-5	8.132484681448542E-5	0.0	0.09372688595369444	2.8463696385069896E-4
56-57	4.066242340724271E-5	8.132484681448542E-5	0.0	0.10501070844920429	2.8463696385069896E-4
58-59	4.066242340724271E-5	8.132484681448542E-5	0.0	0.11686380487241554	2.8463696385069896E-4
60-61	4.066242340724271E-5	8.132484681448542E-5	0.0	0.12934716885843905	2.8463696385069896E-4
62-63	4.066242340724271E-5	8.132484681448542E-5	0.0	0.1400413862145439	2.8463696385069896E-4
64-65	4.066242340724271E-5	8.132484681448542E-5	0.0	0.1507965972057596	3.049681755543203E-4
66-67	4.066242340724271E-5	8.132484681448542E-5	0.0	0.1634832733088193	3.252993872579417E-4
68-69	4.066242340724271E-5	8.132484681448542E-5	0.0	0.17623094304698989	3.659618106651844E-4
70-71	4.066242340724271E-5	8.132484681448542E-5	0.0	0.1889582815734569	3.659618106651844E-4
72-73	4.066242340724271E-5	8.132484681448542E-5	0.0	0.20101469011370432	4.472866574796698E-4
74-75	4.066242340724271E-5	8.132484681448542E-5	0.0	0.21382335348698578	4.472866574796698E-4
76-77	4.066242340724271E-5	8.132484681448542E-5	0.0	0.22705897230604327	4.472866574796698E-4
78-79	4.066242340724271E-5	8.132484681448542E-5	0.0	0.24112817080494925	4.6761786918329113E-4
80-81	4.066242340724271E-5	8.132484681448542E-5	0.0	0.25523803172726245	4.879490808869125E-4
82-83	4.066242340724271E-5	8.132484681448542E-5	0.0	0.26967319203683365	4.879490808869125E-4
84-85	4.066242340724271E-5	8.132484681448542E-5	0.0	0.2850232568730678	4.879490808869125E-4
86	4.066242340724271E-5	8.132484681448542E-5	0.0	0.29691701571968626	4.879490808869125E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	3880	0.0	43.073147	1
TACCTGG	3780	0.0	43.05437	2
ACCTGGG	4765	0.0	32.999847	3
TATAACG	210	1.8189894E-12	28.3795	2
GTATCAA	6080	0.0	26.881685	1
GTACATG	7040	0.0	23.346794	1
TAACGCA	260	4.0017767E-11	22.921906	4
TCGCGTA	140	2.3558727E-5	22.905592	9
TATCAAC	7240	0.0	22.415342	2
TACATGG	7300	0.0	22.293907	2
TCAACGC	7145	0.0	22.007595	4
CAACGCA	7225	0.0	21.954268	5
AACGCAG	7650	0.0	21.2683	6
ATCAACG	7440	0.0	21.258219	3
ACATGGG	7295	0.0	21.05233	3
CCTGGGG	5360	0.0	20.954725	4
ATAACGC	285	1.3460522E-10	20.911211	3
GTGTAGC	1205	0.0	19.485645	1
GTATAGG	540	0.0	18.75688	1
GAGTACA	5515	0.0	18.69968	1
>>END_MODULE