FastQCFastQC Report
Mon 15 Aug 2016
SRR2921458_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921458_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2459273
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27120.11027649228044223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG37350.046.0289841
TACCTGG37000.045.183292
ACCTGGG46000.035.4145743
GTATCAA67300.025.969631
TATAACG2251.0186341E-1025.3300272
GTACATG70150.024.5751191
TACATGG71550.023.3652212
TCAACGC77850.022.2073824
CCTGGGG52400.022.0256944
CAACGCA78700.021.8388255
ACATGGG73350.021.8210433
ATCAACG80300.021.4702383
TATCAAC81350.021.4262142
AACGCAG84600.020.4280286
ATAACGC3052.0918378E-1020.2436713
TAACGCA2851.9699655E-919.9982034
GTATAAC4950.019.241531
GGTATCA50100.018.7259241
CATGGGG60450.018.6997264
TACACGG5350.018.6424262
Produced by FastQC (version 0.11.5)