##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921458_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2459273
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.880607399015886	33.0	31.0	34.0	30.0	34.0
2	31.992431096506976	34.0	31.0	34.0	30.0	34.0
3	32.11887903457648	34.0	31.0	34.0	30.0	34.0
4	35.66989268779839	37.0	35.0	37.0	35.0	37.0
5	35.466036507536984	37.0	35.0	37.0	33.0	37.0
6	35.504402317270184	37.0	35.0	37.0	33.0	37.0
7	35.43441578059858	37.0	35.0	37.0	33.0	37.0
8	35.38604864120413	37.0	35.0	37.0	33.0	37.0
9	36.99610250671642	39.0	37.0	39.0	33.0	39.0
10-11	36.896507829752935	39.0	37.0	39.0	33.0	39.0
12-13	36.77312665165681	39.0	37.0	39.0	33.0	39.0
14-15	38.05089512225767	40.0	38.0	41.0	33.0	41.0
16-17	37.95433589520155	40.0	38.0	41.0	33.0	41.0
18-19	37.84528740810801	40.0	38.0	41.0	33.0	41.0
20-21	37.72055623755476	40.0	38.0	41.0	32.0	41.0
22-23	37.68035573927742	40.0	38.0	41.0	32.5	41.0
24-25	37.73401285664504	40.0	38.0	41.0	32.5	41.0
26-27	37.61372242935209	40.0	38.0	41.0	32.0	41.0
28-29	37.47014239574053	40.0	38.0	41.0	32.0	41.0
30-31	37.32585991876461	40.0	37.0	41.0	32.0	41.0
32-33	37.15317331585391	40.0	37.0	41.0	31.0	41.0
34-35	36.99674619287896	40.0	37.0	41.0	31.0	41.0
36-37	36.88740696945805	40.0	37.0	41.0	30.5	41.0
38-39	36.84680675955862	40.0	37.0	41.0	30.5	41.0
40-41	36.672700224822535	40.0	36.0	41.0	30.0	41.0
42-43	36.42261290226827	39.0	36.0	41.0	30.0	41.0
44-45	36.203144994476006	39.0	35.0	40.5	30.0	41.0
46-47	35.91078867616568	39.0	35.0	40.0	29.0	41.0
48-49	35.554343499074726	39.0	35.0	40.0	28.0	41.0
50-51	35.26031778497141	38.0	34.5	40.0	27.0	41.0
52-53	34.925418202859134	38.0	34.0	40.0	26.0	41.0
54-55	34.581124381067085	38.0	34.0	40.0	26.0	41.0
56-57	34.17535426119833	37.5	33.0	40.0	24.5	41.0
58-59	33.76371452051073	37.0	33.0	40.0	23.5	41.0
60-61	33.311003089124306	36.0	32.5	39.0	22.5	40.5
62-63	32.84663719725301	36.0	32.0	39.0	21.5	40.0
64-65	32.30423665855723	35.0	31.0	38.5	20.0	40.0
66-67	31.751066270397796	35.0	31.0	38.0	18.0	40.0
68-69	31.221958684538073	35.0	30.0	37.0	15.5	39.5
70-71	30.64358613297507	34.0	29.5	36.5	11.5	39.0
72-73	30.06886161072805	34.0	29.0	36.0	8.0	39.0
74-75	29.52974517265875	34.0	29.0	35.5	7.0	38.0
76-77	28.306717879633535	32.0	27.0	34.5	4.5	36.0
78-79	29.230391867840616	33.5	29.0	35.0	3.5	37.0
80-81	29.243747644120845	34.0	29.0	35.0	2.0	36.5
82-83	28.879362315611157	34.0	29.0	35.0	2.0	36.0
84-85	28.3508331527244	33.5	28.5	35.0	2.0	35.5
86-87	27.79342187711572	33.0	27.0	35.0	2.0	35.0
88-89	27.218214488590736	33.0	26.0	35.0	2.0	35.0
90-91	26.68905017865036	32.0	25.0	34.0	2.0	35.0
92-93	26.201240163251498	32.0	24.5	34.0	2.0	35.0
94-95	25.645293344821823	32.0	23.5	34.0	2.0	35.0
96-97	24.80674004065429	31.5	19.0	34.0	2.0	35.0
98-99	23.721555923234227	31.0	2.0	34.0	2.0	35.0
100-101	20.16818913556974	25.5	2.0	32.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	16307.0
3	8592.0
4	5294.0
5	3955.0
6	3440.0
7	3587.0
8	4279.0
9	5196.0
10	6044.0
11	7180.0
12	8715.0
13	10207.0
14	11481.0
15	12818.0
16	14627.0
17	17312.0
18	17819.0
19	15892.0
20	16219.0
21	16934.0
22	18231.0
23	20109.0
24	22846.0
25	26508.0
26	31063.0
27	38195.0
28	45901.0
29	54744.0
30	67290.0
31	83689.0
32	105991.0
33	139655.0
34	193411.0
35	284379.0
36	422279.0
37	491712.0
38	201257.0
39	6115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.92115932098312	17.1451761577303	13.176508495868308	24.75715602541827
2	16.36857721773874	18.85142479098498	38.400291468250984	26.3797065230253
3	20.655123868607618	22.565224074452107	27.123487986440775	29.656164070499504
4	14.092542820013948	14.604088295198316	34.00225237870884	37.3011165060789
5	15.769508369370525	35.18864376144905	32.30290350978849	16.738944359391933
6	35.674830026080976	32.57632042575873	16.456608333706306	15.29224121445399
7	32.043128540450404	29.64690409852976	18.929876040237787	19.38009132078205
8	28.470710824468465	33.479309425491344	18.44997242342157	19.600007326618623
9	27.043390103948546	16.723705893293708	18.627184044807642	37.60571995795011
10-11	26.975234187188168	24.807632861075422	25.72635116916037	22.49078178257604
12-13	27.036486071230154	21.793310039011544	26.521684273076744	24.648519616681554
14-15	23.92584593701226	23.394526208545383	24.051998007932465	28.627629846509894
16-17	24.505169834014964	26.522278619671912	24.543629111822536	24.428922434490588
18-19	24.555505974111977	25.850700284783702	26.011030944651242	23.58276279645308
20-21	24.970913638163424	24.92838989574057	25.995042353932163	24.105654112163844
22-23	24.676519145254332	24.700499146055694	25.888475301107654	24.73450640758232
24-25	24.44367657502943	25.00471839711169	25.98232146764929	24.569283560209595
26-27	24.5843153602912	25.021687629002248	26.06639485109455	24.327602159612002
28-29	24.487243962608673	25.30847112811921	25.88495498736425	24.31932992190787
30-31	24.685252950454966	25.089490593880846	25.811169255655404	24.414087200008783
32-33	24.382432338739076	25.128200123335624	25.91444948210627	24.57491805581903
34-35	24.59539312292847	25.15405631648689	25.980746086602558	24.269804473982084
36-37	24.525598293935076	25.31401078165362	25.826395531177006	24.3339953932343
38-39	24.586768708824806	25.175335817147264	25.902219449475016	24.33567602455291
40-41	24.688992568765304	25.10568914569576	25.81251358671614	24.392804698822793
42-43	24.599076283468253	25.118514343712334	25.801684778277128	24.48072459454228
44-45	24.59904911289985	25.08892961963296	25.75764538523697	24.55437588223022
46-47	24.683283927313873	25.230197072975507	25.72521305392648	24.36130594578414
48-49	24.651714392077665	25.290269351171478	25.579669525901355	24.478346730849502
50-51	24.518974166700048	25.387159943288047	25.534836312649357	24.559029577362555
52-53	24.638385696576815	25.55853685860389	25.357700453660936	24.44537699115836
54-55	24.673266400725442	25.113147011820963	25.507463839719584	24.70612274773401
56-57	24.58078625171652	25.35321632718455	25.517457598791797	24.548539822307127
58-59	24.81608183971835	25.263861362385054	25.476406183033333	24.443650614863255
60-61	24.82322641353298	25.20610284413487	25.465298252626873	24.50537248970528
62-63	24.55704310411439	25.24173115948807	25.69408238542781	24.50714335096973
64-65	24.56636357000573	25.24699419486633	25.542262841146403	24.644379393981538
66-67	24.612520915887572	25.446288651553466	25.44295309248021	24.498237340078756
68-69	24.622985236767104	25.394855958287817	25.440710067357124	24.54144873758796
70-71	24.62106424095727	25.1676499964213	25.586480151216435	24.624805611404998
72-73	24.84540389009084	25.121405180306876	25.426794272774938	24.606396656827346
74-75	24.46214910905876	25.471487215559186	25.38123261703959	24.68513105834247
76-77	24.66882677280958	25.1707353876772	25.58728650429846	24.57315133521476
78-79	24.60269517626806	25.122885803640298	25.629139301910563	24.645279718181076
80-81	24.599622405973168	25.377344887176346	25.408726314699788	24.6143063921507
82-83	24.660980308976573	25.386932494601677	25.29761456874613	24.654472627675624
84-85	24.680361643031137	25.313405726739933	25.380571722322802	24.625660907906127
86-87	24.586427338367603	25.334443616458923	25.497281522925235	24.58184752224824
88-89	24.662020353369947	25.168688393331184	25.376726172010866	24.792565081288004
90-91	24.60145100132446	25.226894540402924	25.355106089051045	24.816548369221575
92-93	24.718959422675347	25.481134389733196	25.45125355101934	24.348652636572112
94-95	24.649496913978748	25.22241617520427	25.42322359344834	24.70486331736864
96-97	24.58100604094652	25.301076455964488	25.470505984930874	24.647411518158115
98-99	24.73181291177632	25.32379887686554	25.336242904159434	24.608145307198708
100-101	25.132783236678062	24.625134653852676	25.52215602320052	24.719926086268742
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	132.0
1	118.5
2	233.0
3	345.5
4	236.0
5	164.0
6	310.5
7	708.5
8	1074.5
9	1030.0
10	756.5
11	565.0
12	522.5
13	560.0
14	603.5
15	644.5
16	705.5
17	783.0
18	862.5
19	959.0
20	1034.5
21	1137.0
22	1292.5
23	1557.0
24	1852.5
25	2272.5
26	2899.0
27	3715.5
28	4840.0
29	6371.0
30	8429.0
31	10777.0
32	13782.5
33	17361.5
34	21000.0
35	25216.0
36	30471.0
37	35718.5
38	41009.5
39	47073.0
40	53176.0
41	58718.5
42	64770.5
43	72453.0
44	80866.5
45	89727.0
46	95252.5
47	100753.5
48	110155.5
49	113817.5
50	111059.0
51	108953.5
52	109309.0
53	109138.5
54	108602.5
55	105867.0
56	99933.5
57	93012.5
58	85839.5
59	79089.0
60	69263.0
61	58112.0
62	47328.5
63	36175.5
64	26817.5
65	20048.5
66	15181.0
67	11709.0
68	9012.5
69	6818.5
70	5139.0
71	3976.0
72	2973.5
73	2202.5
74	1685.0
75	1205.5
76	837.0
77	524.0
78	309.5
79	184.0
80	103.5
81	55.5
82	31.5
83	11.5
84	4.5
85	4.0
86	3.0
87	2.5
88	1.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3046835385904696
2	0.0
3	0.005489427159977765
4	0.05802527820213534
5	3.252993872579417E-4
6	0.0627421193173755
7	0.07246043851170651
8	0.10076148520314744
9	0.08913203210867601
10-11	0.09423516624628497
12-13	0.09384887322391616
14-15	0.06201019569604513
16-17	0.03368881779290059
18-19	0.037246779841034326
20-21	0.026288256732782414
22-23	0.03962553161035802
24-25	0.03297722538327384
26-27	0.02024988685680687
28-29	0.023360562247460935
30-31	0.003090344178950446
32-33	0.0052657838312379305
34-35	0.00766486681226525
36-37	0.011426140977435202
38-39	0.017078217831041936
40-41	0.021530753194135017
42-43	0.003131006602357689
44-45	0.01244270156261627
46-47	0.02295393801338851
48-49	0.0019721275352512714
50-51	0.007298905001600066
52-53	0.002338089345916456
54-55	0.003374981142801145
56-57	0.003496968413022873
58-59	0.001097885431995553
60-61	0.0050624717142017175
62-63	0.01394721122868425
64-65	0.006363669263233484
66-67	0.03718578620592346
68-69	0.060099061795904726
70-71	0.011426140977435202
72-73	0.03131006602357689
74-75	0.049872462308983186
76-77	0.29411130850458655
78-79	0.07353799273199844
80-81	0.033038219018384704
82-83	0.026023950980635334
84-85	0.01787113508748317
86-87	0.1155829385350874
88-89	0.5124278597780726
90-91	0.12914385674140286
92-93	0.04781900992691743
94-95	0.008152815893152164
96-97	0.005631745641903115
98-99	0.01065355493269759
100-101	0.007725860447376115
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2459273.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.726697646025976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.2463603569886	46.13670009297825
2	13.416748666809857	16.02676182030549
3	4.06682345591261	7.286938047931766
4	1.7198405546314461	4.10881587223384
5	0.9217931564293335	2.752783057311536
6	0.6052614408737097	2.1690160245517247
7	0.39169287846392475	1.6376165485481504
8	0.2831828564780825	1.3530861477923521
9	0.1968312722350635	1.0580473695659638
>10	1.06511764765635	11.22206440856724
>50	0.05914035297727617	2.4327874854602083
>100	0.02529699991674537	2.73241557088465
>500	0.0014327704702524874	0.5727388986174268
>1k	4.775901567482888E-4	0.5102286552514246
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2712	0.11027649228044223	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.066242340724271E-5	0.0
10-11	0.0	0.0	0.0	4.066242340724271E-5	0.0
12-13	0.0	0.0	0.0	8.132484681448542E-5	0.0
14-15	0.0	0.0	0.0	8.132484681448542E-5	0.0
16-17	0.0	0.0	0.0	1.0165605851810677E-4	0.0
18-19	0.0	2.0331211703621355E-5	0.0	1.2198727022172813E-4	0.0
20-21	0.0	4.066242340724271E-5	0.0	1.2198727022172813E-4	0.0
22-23	0.0	4.066242340724271E-5	0.0	1.6264969362897084E-4	0.0
24-25	0.0	4.066242340724271E-5	0.0	1.6264969362897084E-4	4.066242340724271E-5
26-27	0.0	4.066242340724271E-5	0.0	2.4397454044345626E-4	4.066242340724271E-5
28-29	0.0	4.066242340724271E-5	0.0	5.489427159977767E-4	4.066242340724271E-5
30-31	0.0	4.066242340724271E-5	0.0	0.0018501402650295434	4.066242340724271E-5
32-33	0.0	4.066242340724271E-5	0.0	0.004167898399242378	4.066242340724271E-5
34-35	0.0	4.066242340724271E-5	0.0	0.0077461916590797365	4.066242340724271E-5
36-37	0.0	4.066242340724271E-5	0.0	0.012259720657283677	4.066242340724271E-5
38-39	0.0	4.066242340724271E-5	0.0	0.02041253655043584	4.066242340724271E-5
40-41	0.0	4.066242340724271E-5	0.0	0.028931314254253188	4.066242340724271E-5
42-43	0.0	4.066242340724271E-5	0.0	0.0372264486293307	4.066242340724271E-5
44-45	0.0	4.066242340724271E-5	0.0	0.04582655117996253	4.066242340724271E-5
46-47	0.0	4.066242340724271E-5	0.0	0.05434532888377988	4.066242340724271E-5
48-49	0.0	4.066242340724271E-5	0.0	0.06300642506952257	4.066242340724271E-5
50-51	0.0	4.066242340724271E-5	0.0	0.07223679518296668	4.066242340724271E-5
52-53	0.0	4.066242340724271E-5	0.0	0.08270736921033167	4.066242340724271E-5
54-55	0.0	4.066242340724271E-5	0.0	0.09258833809829164	4.066242340724271E-5
56-57	0.0	4.066242340724271E-5	0.0	0.10360785484165441	4.066242340724271E-5
58-59	0.0	4.066242340724271E-5	0.0	0.11509498945420049	4.066242340724271E-5
60-61	0.0	4.066242340724271E-5	0.0	0.12737504132318778	4.066242340724271E-5
62-63	0.0	4.066242340724271E-5	0.0	0.13760164081010934	4.066242340724271E-5
64-65	0.0	4.066242340724271E-5	0.0	0.1479502275672526	4.066242340724271E-5
66-67	0.0	4.066242340724271E-5	0.0	0.16010829216601818	4.066242340724271E-5
68-69	0.0	4.066242340724271E-5	0.0	0.17275430584567064	4.066242340724271E-5
70-71	0.0	4.066242340724271E-5	0.0	0.1851766761965833	4.066242340724271E-5
72-73	0.0	4.066242340724271E-5	0.0	0.19625718657505692	1.0165605851810677E-4
74-75	0.0	4.066242340724271E-5	0.0	0.20874055056108046	1.2198727022172813E-4
76-77	0.0	4.066242340724271E-5	0.0	0.22140689545243655	1.2198727022172813E-4
78-79	0.0	6.0993635110864064E-5	0.0	0.23513046335238097	1.6264969362897084E-4
80-81	0.0	8.132484681448542E-5	0.0	0.24875237519380727	1.6264969362897084E-4
82-83	4.066242340724271E-5	8.132484681448542E-5	0.0	0.2627605800576024	2.0331211703621355E-4
84-85	4.066242340724271E-5	8.132484681448542E-5	0.0	0.2771550779437663	2.0331211703621355E-4
86-87	4.066242340724271E-5	8.132484681448542E-5	0.0	0.29242381793318595	2.0331211703621355E-4
88-89	4.066242340724271E-5	8.132484681448542E-5	0.0	0.30883110577800843	2.0331211703621355E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	3735	0.0	46.028984	1
TACCTGG	3700	0.0	45.18329	2
ACCTGGG	4600	0.0	35.414574	3
GTATCAA	6730	0.0	25.96963	1
TATAACG	225	1.0186341E-10	25.330027	2
GTACATG	7015	0.0	24.575119	1
TACATGG	7155	0.0	23.365221	2
TCAACGC	7785	0.0	22.207382	4
CCTGGGG	5240	0.0	22.025694	4
CAACGCA	7870	0.0	21.838825	5
ACATGGG	7335	0.0	21.821043	3
ATCAACG	8030	0.0	21.470238	3
TATCAAC	8135	0.0	21.426214	2
AACGCAG	8460	0.0	20.428028	6
ATAACGC	305	2.0918378E-10	20.243671	3
TAACGCA	285	1.9699655E-9	19.998203	4
GTATAAC	495	0.0	19.24153	1
GGTATCA	5010	0.0	18.725924	1
CATGGGG	6045	0.0	18.699726	4
TACACGG	535	0.0	18.642426	2
>>END_MODULE