FastQCFastQC Report
Mon 15 Aug 2016
SRR2921457_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921457_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2693392
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG32650.034.5428541
TACCTGG35800.031.2477762
GTATCAA48600.029.4577431
GGTATCA31850.027.46121
TCAACGC53200.025.68134
GTACATG82000.024.8693941
CAACGCA55250.024.5624545
ACCTGGG43550.024.213193
ATCAACG57000.023.8887793
TACATGG83250.023.5706652
AACGCAG59050.023.2858816
TATCAAC58400.023.2375972
ACATGGG80700.022.895173
GAGTACA60400.020.7303811
GTATAGG7550.020.7303811
AGTACAT56750.020.6009222
CATGGGG62700.019.7427924
ACGCAGA69550.019.5657887
GTGTAGC15300.019.2559031
CGCAGAG73500.018.4481878
Produced by FastQC (version 0.11.5)