##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921457_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2693392
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.393818649494765	31.0	31.0	34.0	27.0	34.0
2	30.493484424101652	31.0	31.0	34.0	27.0	34.0
3	30.62905213945835	31.0	31.0	34.0	27.0	34.0
4	34.12438144911695	37.0	35.0	37.0	30.0	37.0
5	34.11784545287132	37.0	35.0	37.0	30.0	37.0
6	34.211013844252896	37.0	35.0	37.0	30.0	37.0
7	34.096837370869146	37.0	35.0	37.0	30.0	37.0
8	34.07838108971884	37.0	35.0	37.0	30.0	37.0
9	35.54060864515822	39.0	37.0	39.0	30.0	39.0
10-11	35.413911528659774	39.0	36.0	39.0	30.0	39.0
12-13	35.25378351907186	39.0	35.0	39.0	30.0	39.0
14-15	36.28958892727089	40.0	36.5	41.0	29.0	41.0
16-17	36.14044595068226	40.0	36.0	41.0	28.5	41.0
18-19	35.959297049965244	39.0	36.0	41.0	27.5	41.0
20-21	35.847350478504424	39.0	36.0	41.0	27.0	41.0
22-23	35.89622453768334	39.0	36.0	41.0	27.0	41.0
24-25	35.76710204084664	39.0	36.0	41.0	27.0	41.0
26-27	35.505715840843074	39.0	36.0	41.0	26.5	41.0
28-29	35.276747127785335	39.0	35.0	40.0	25.5	41.0
30-31	35.036509353261614	39.0	35.0	40.0	24.5	41.0
32-33	34.77888755145928	38.5	34.5	40.0	24.0	41.0
34-35	34.37033042349572	38.0	34.0	40.0	21.5	41.0
36-37	34.00339905962444	38.0	33.5	40.0	18.5	41.0
38-39	33.77545878951152	38.0	33.0	40.0	17.5	41.0
40-41	33.51671052709743	38.0	33.0	40.0	15.5	41.0
42-43	33.24367043490142	38.0	33.0	40.0	15.0	41.0
44-45	32.93822102389849	38.0	32.0	40.0	11.0	41.0
46-47	32.66607367215764	38.0	32.0	40.0	9.0	41.0
48-49	32.469220039266474	37.0	31.0	40.0	8.5	41.0
50-51	31.724857168952756	36.0	30.5	39.0	8.0	40.0
52-53	32.218356629855585	37.0	31.5	39.0	7.5	40.0
54-55	32.651790938712224	37.0	32.0	40.0	7.0	41.0
56-57	32.508966388850936	37.0	32.0	40.0	2.0	41.0
58-59	32.181100263162584	37.0	31.5	40.0	2.0	41.0
60-61	31.726032824037496	36.0	31.0	39.0	2.0	41.0
62-63	31.24932241574936	35.5	30.5	39.0	2.0	40.0
64-65	30.712650999186156	35.0	30.0	38.5	2.0	40.0
66-67	30.226987011174018	35.0	29.0	38.0	2.0	40.0
68-69	29.711482769682245	34.5	29.0	37.5	2.0	39.5
70-71	29.184136768803057	34.0	28.0	37.0	2.0	39.0
72-73	28.62294961149361	34.0	27.5	36.0	2.0	39.0
74-75	28.02094366508848	34.0	26.0	35.5	2.0	38.0
76-77	27.43440019128296	33.0	26.0	35.0	2.0	37.0
78-79	26.865310173936805	33.0	25.0	35.0	2.0	37.0
80-81	26.28510740360111	32.0	23.5	35.0	2.0	36.0
82-83	25.654273124743817	32.0	21.0	34.5	2.0	35.5
84-85	25.030910094037555	31.5	19.0	34.0	2.0	35.0
86-87	24.40930711162727	31.0	16.5	34.0	2.0	35.0
88-89	23.76131584262521	31.0	7.0	34.0	2.0	35.0
90-91	23.017272272287137	30.0	2.0	34.0	2.0	35.0
92-93	22.198177613952964	29.5	2.0	34.0	2.0	35.0
94-95	21.28362228743532	29.0	2.0	34.0	2.0	35.0
96-97	20.39308407391126	29.0	2.0	33.5	2.0	34.5
98	19.76965996780268	27.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	84998.0
3	14723.0
4	11385.0
5	9370.0
6	8843.0
7	9272.0
8	10147.0
9	12118.0
10	13632.0
11	14607.0
12	16061.0
13	18811.0
14	15790.0
15	15543.0
16	17117.0
17	19333.0
18	21603.0
19	21882.0
20	22919.0
21	24615.0
22	27070.0
23	30431.0
24	33830.0
25	37633.0
26	42795.0
27	48765.0
28	56318.0
29	66388.0
30	78330.0
31	95365.0
32	118476.0
33	152620.0
34	205912.0
35	287395.0
36	398419.0
37	429618.0
38	192756.0
39	8502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.60019159039049	16.7253263023838	12.567150827818955	25.107331279406758
2	16.220279277375084	18.271379213891134	38.876062910402936	26.632278598330846
3	20.530764755662908	21.72724415579977	27.59042786760155	30.151563220935774
4	13.659760686808797	14.246341762417947	34.52681593093277	37.56708161984048
5	15.254511841354384	35.68976483735971	33.06662545177858	15.989097869507324
6	35.69727761626557	32.824350055265036	16.488858753703685	14.989513574765704
7	31.842158536064435	29.91224632688197	18.806512901182863	19.439082235870732
8	28.37964150976328	34.02701210230419	18.332526859658355	19.260819528274176
9	26.964543125964997	16.196141280897493	18.484651792152892	38.35466380098462
10-11	27.06371103700788	24.73465803174226	25.891590603578248	22.310040327671615
12-13	26.88035730464915	21.65162425382057	26.513710095460468	24.954308346069812
14-15	23.88989965812502	23.328896428759087	23.90902371327137	28.87218019984452
16-17	24.555332464155708	26.521936585186523	24.453270314860895	24.469460635796874
18-19	24.540318530208065	25.797960075248973	25.823964360755227	23.83775703378774
20-21	24.987374783680526	25.030146303868055	25.79880737691668	24.183671535534735
22-23	24.745080046287082	24.825471052083373	25.580042663354398	24.84940623827515
24-25	24.572778146334503	25.040429258875257	25.722204501362565	24.664588093427675
26-27	24.650842426375252	25.156792495811004	25.714526731369315	24.477838346444432
28-29	24.515976671747826	25.373041702929704	25.62859858802684	24.482383037295623
30-31	24.7339347088453	25.266483231222836	25.535599484874933	24.463982575056928
32-33	24.5588020094769	25.202492755647103	25.50966743201896	24.729037802857036
34-35	24.690605362293667	25.256946655145757	25.633438925125752	24.419009057434828
36-37	24.755552525174217	25.248809355908016	25.467445142573737	24.528192976344034
38-39	24.694175256682925	25.244294778476256	25.571409020324587	24.49012094451623
40-41	24.81268323046072	25.150939052902366	25.581348023570744	24.455029693066166
42-43	24.749196714625814	25.072452001190815	25.547918550642674	24.6304327335407
44-45	24.724634684440737	25.197377334554066	25.468814325154828	24.609173655850366
46-47	24.757478572072348	25.243314484706225	25.476081314257428	24.523125628964003
48-49	24.74799029407896	25.296928977174666	25.3620455946929	24.593035134053473
50-51	24.58486181094413	25.44630177774278	25.343611221728374	24.625225189584718
52-53	24.711021981061442	25.419831232690875	25.317188078079766	24.551958708167916
54-55	24.672019309543423	25.16724830791313	25.472395254165896	24.688337128377547
56-57	24.600253807106597	25.38754105703195	25.457953866825918	24.554251269035532
58-59	24.731394052970575	25.37608564194046	25.407151294579442	24.48536901050952
60-61	24.798531039077123	25.17638914028838	25.453312159794283	24.57176766084021
62-63	24.627748252280725	25.325641545805183	25.497973319368622	24.548636882545463
64-65	24.630854693834888	25.2308278143223	25.482039259021438	24.65627823282138
66-67	24.618162298410624	25.405204565080403	25.342544402175182	24.634088734333794
68-69	24.64755718027828	25.32135096175739	25.39534809070611	24.63574376725822
70-71	24.60104004251345	25.17086426044375	25.494788689020055	24.733307008022752
72-73	24.762807588292198	25.15986114874738	25.39785083803665	24.679480424923774
74-75	24.542361883487924	25.378719722699504	25.356624437013053	24.722293956799522
76-77	24.681651690780445	25.198968276035956	25.373222283700965	24.746157749482638
78-79	24.69386761833883	25.119535541416145	25.475965062671662	24.710631777573358
80-81	24.604402778303513	25.27035576851896	25.406477648694043	24.718763804483483
82-83	24.784845643161578	25.256969203235073	25.332765606337652	24.625419547265697
84-85	24.723432920166154	25.23574506103913	25.28153582521352	24.7592861935812
86-87	24.710023669444418	25.186768603446847	25.496829935181708	24.60637779192703
88-89	24.691112349892325	25.044811532327806	25.388588860555238	24.87548725722463
90-91	24.67150137873577	25.13988741878437	25.336069174720173	24.852542027759693
92-93	24.73632120203895	25.23658653098531	25.443144090212588	24.58394817676315
94-95	24.811306452121208	25.089104380002652	25.351107527397044	24.748481640479095
96-97	24.796447421294403	25.10792272414092	25.348950607557715	24.746679247006963
98	24.773946460169565	25.26634732459338	25.20668045220031	24.753025763036746
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	280.0
1	196.5
2	173.5
3	226.5
4	195.0
5	183.5
6	250.5
7	443.0
8	661.5
9	744.0
10	745.5
11	803.5
12	930.0
13	1073.0
14	1279.5
15	1511.0
16	1669.5
17	1714.0
18	1757.0
19	1734.0
20	1574.0
21	1429.5
22	1371.0
23	1416.0
24	1464.5
25	1643.5
26	2031.5
27	2706.5
28	3764.0
29	5342.0
30	7639.0
31	10709.5
32	14716.0
33	19399.5
34	24741.5
35	31049.5
36	38351.0
37	45820.0
38	53211.5
39	60904.5
40	68421.5
41	75889.0
42	84134.0
43	93290.5
44	101392.5
45	108264.5
46	116227.0
47	124263.5
48	127651.0
49	126668.5
50	125867.0
51	125243.5
52	124759.5
53	124735.5
54	122555.0
55	116367.0
56	107678.5
57	99848.0
58	91982.5
59	82706.5
60	72145.5
61	60186.5
62	48332.0
63	36807.5
64	26817.0
65	20007.5
66	15330.5
67	11570.0
68	8737.0
69	6707.5
70	4956.0
71	3688.0
72	2959.0
73	2251.0
74	1690.0
75	1246.5
76	803.0
77	516.5
78	311.5
79	181.0
80	111.0
81	71.5
82	49.0
83	29.0
84	20.5
85	14.5
86	9.5
87	6.5
88	8.0
89	7.0
90	4.5
91	3.5
92	3.0
93	4.0
94	3.5
95	4.0
96	4.0
97	1.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47052192922530406
2	0.05097661239062119
3	0.05858783274027694
4	0.05650867010817586
5	0.06712725069354925
6	0.06746140183085121
7	0.044442101261160646
8	0.022239614582652657
9	0.01388583615010366
10-11	0.01678181267338731
12-13	0.012623487409185147
14-15	0.016651865008880995
16-17	0.015779359261481433
18-19	0.056731437533043834
20-21	0.0872134468358115
22-23	0.10367967232396919
24-25	0.07286351188389956
26-27	0.0896638885093592
28-29	0.08932973737205724
30-31	0.060091512858135764
32-33	0.035271508937429086
34-35	0.398530923088804
36-37	0.6235445861575292
38-39	0.5728278690959208
40-41	0.5658849510208688
42-43	0.5401924413527626
44-45	0.5247286692765107
46-47	0.5155766409048516
48-49	0.5043640138531637
50-51	0.5191594836548115
52-53	0.5998198554090901
54-55	0.5694677937708287
56-57	0.5269192156210458
58-59	0.5043268859490189
60-61	0.5178228791056037
62-63	0.5293510933425213
64-65	0.5484534000249499
66-67	0.5737931946036819
68-69	0.5284785875951217
70-71	0.476295318319799
72-73	0.4601446800168709
74-75	0.43901890255855813
76-77	0.4547611339158949
78-79	0.448375134403013
80-81	0.44447670446782345
82-83	0.46506412731603863
84-85	0.43174183334620436
86-87	0.4330784378954121
88-89	0.4619268194158147
90-91	0.44367845452871324
92-93	0.4386661874691838
94-95	0.4502315296102461
96-97	0.4440311696180875
98	0.4394830013603664
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2693392.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.389287247726834
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.35117626741938	48.88274042345305
2	12.966738582355353	16.179711561615008
3	3.918593088328625	7.334346893840747
4	1.6279089151022466	4.062563076697977
5	0.8613604167273167	2.6869831231511125
6	0.5137717548399114	1.9232312155485554
7	0.34177378653964	1.492611605951541
8	0.23666743821568106	1.1812410228017414
9	0.17945986422970922	1.0076735716978849
>10	0.9250487414528599	10.253374417151644
>50	0.05515151444245582	2.3361014339728774
>100	0.0216318025587243	2.2541941806064396
>500	5.383708410570536E-4	0.2529219550551118
>1k	1.7945694701846748E-4	0.15230551845616003
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.7127904144662194E-5	0.0	0.0	0.0	0.0
3	3.7127904144662194E-5	0.0	0.0	0.0	0.0
4	3.7127904144662194E-5	0.0	0.0	0.0	0.0
5	3.7127904144662194E-5	0.0	0.0	0.0	0.0
6	3.7127904144662194E-5	0.0	0.0	0.0	0.0
7	3.7127904144662194E-5	0.0	0.0	0.0	0.0
8	3.7127904144662194E-5	0.0	0.0	0.0	0.0
9	3.7127904144662194E-5	0.0	0.0	3.7127904144662194E-5	0.0
10-11	3.7127904144662194E-5	0.0	0.0	5.5691856216993294E-5	7.425580828932439E-5
12-13	3.7127904144662194E-5	0.0	0.0	9.281976036165548E-5	2.2276742486797318E-4
14-15	5.5691856216993294E-5	0.0	0.0	1.1138371243398659E-4	3.3415113730195977E-4
16-17	1.1138371243398659E-4	0.0	0.0	1.1138371243398659E-4	4.0840694559128414E-4
18-19	1.4851161657864878E-4	3.7127904144662194E-5	0.0	1.6707556865097988E-4	4.0840694559128414E-4
20-21	1.85639520723311E-4	5.5691856216993294E-5	0.0	1.85639520723311E-4	4.6409880180827746E-4
22-23	1.85639520723311E-4	7.425580828932439E-5	0.0	2.2276742486797318E-4	5.383546100976018E-4
24-25	2.784592810849665E-4	7.425580828932439E-5	0.0	2.784592810849665E-4	5.569185621699329E-4
26-27	2.9702323315729755E-4	7.425580828932439E-5	0.0	4.6409880180827746E-4	5.940464663145951E-4
28-29	2.9702323315729755E-4	7.425580828932439E-5	0.0	0.0012623487409185146	5.940464663145951E-4
30-31	2.9702323315729755E-4	7.425580828932439E-5	0.0	0.004176889216274497	6.311743704592573E-4
32-33	2.9702323315729755E-4	7.425580828932439E-5	0.0	0.009244848132020887	7.42558082893244E-4
34-35	2.9702323315729755E-4	7.425580828932439E-5	0.0	0.015278132555528495	7.982499391102372E-4
36-37	2.9702323315729755E-4	9.281976036165548E-5	0.0	0.02500564344142999	8.168138911825683E-4
38-39	2.9702323315729755E-4	1.1138371243398659E-4	0.0	0.041676072402383316	9.281976036165549E-4
40-41	2.9702323315729755E-4	1.1138371243398659E-4	0.0	0.058884855973434236	9.281976036165549E-4
42-43	2.9702323315729755E-4	1.1138371243398659E-4	0.0	0.07553672098231523	9.281976036165549E-4
44-45	2.9702323315729755E-4	1.299476645063177E-4	0.0	0.09326529521139143	0.0010395813160505416
46-47	2.9702323315729755E-4	1.4851161657864878E-4	0.0	0.11138371243398659	0.0012066568847015212
48-49	2.9702323315729755E-4	1.4851161657864878E-4	0.0	0.13146990857624885	0.0012623487409185146
50-51	2.9702323315729755E-4	1.4851161657864878E-4	0.0	0.1510920059167028	0.001336604549207839
52-53	2.9702323315729755E-4	1.4851161657864878E-4	0.0	0.17075123116130142	0.001503680117858819
54-55	2.9702323315729755E-4	2.0420347279564207E-4	0.0	0.1890552879046199	0.0015593719740758121
56-57	2.9702323315729755E-4	2.2276742486797318E-4	0.0	0.20930855961553313	0.0016707556865097988
58-59	2.9702323315729755E-4	2.4133137694030428E-4	0.0	0.229821726655459	0.0017821393989437854
60-61	2.9702323315729755E-4	2.598953290126354E-4	0.0	0.24964802746870862	0.0017821393989437854
62-63	2.9702323315729755E-4	2.784592810849665E-4	0.0	0.26943720037781355	0.0018192673030884476
64-65	2.9702323315729755E-4	3.3415113730195977E-4	0.0	0.290377338315403	0.0018192673030884476
66-67	2.9702323315729755E-4	3.3415113730195977E-4	0.0	0.31478893529051843	0.0018563952072331098
68-69	3.3415113730195977E-4	3.3415113730195977E-4	0.0	0.3385322299910299	0.0018563952072331098
70-71	3.3415113730195977E-4	3.712790414466219E-4	0.0	0.36283244325371133	0.001893523111377772
72-73	3.3415113730195977E-4	4.0840694559128414E-4	0.0	0.3867056856187291	0.002060598680028752
74-75	3.3415113730195977E-4	4.0840694559128414E-4	0.0	0.409465090859407	0.0022091102966074003
76-77	3.71279041446622E-4	4.0840694559128414E-4	0.0	0.43432222268425835	0.0022276742486797316
78-79	3.71279041446622E-4	4.0840694559128414E-4	0.0	0.46118426133292145	0.002301930056969056
80-81	3.71279041446622E-4	4.0840694559128414E-4	0.0	0.48533596297902426	0.0023947498173307116
82-83	3.71279041446622E-4	4.2697089766361525E-4	0.0	0.5110656005512751	0.0025246974818370293
84-85	3.71279041446622E-4	4.4553484973594635E-4	0.0	0.5363497032737901	0.0025432614339093605
86	3.71279041446622E-4	4.4553484973594635E-4	0.0	0.5568071784574989	0.0025618253859816917
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	3265	0.0	34.542854	1
TACCTGG	3580	0.0	31.247776	2
GTATCAA	4860	0.0	29.457743	1
GGTATCA	3185	0.0	27.4612	1
TCAACGC	5320	0.0	25.6813	4
GTACATG	8200	0.0	24.869394	1
CAACGCA	5525	0.0	24.562454	5
ACCTGGG	4355	0.0	24.21319	3
ATCAACG	5700	0.0	23.888779	3
TACATGG	8325	0.0	23.570665	2
AACGCAG	5905	0.0	23.285881	6
TATCAAC	5840	0.0	23.237597	2
ACATGGG	8070	0.0	22.89517	3
GAGTACA	6040	0.0	20.730381	1
GTATAGG	755	0.0	20.730381	1
AGTACAT	5675	0.0	20.600922	2
CATGGGG	6270	0.0	19.742792	4
ACGCAGA	6955	0.0	19.565788	7
GTGTAGC	1530	0.0	19.255903	1
CGCAGAG	7350	0.0	18.448187	8
>>END_MODULE