FastQCFastQC Report
Mon 15 Aug 2016
SRR2921457_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921457_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2693392
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG35250.032.9707641
TACCTGG35950.031.4423432
GTATCAA50950.027.578851
GGTATCA36300.027.2932341
GTACATG83800.025.5211731
ACCTGGG44550.024.627783
TACATGG86850.023.9519652
TATACCG1801.9643267E-723.735865
ACATGGG84750.023.369933
TCAACGC59250.023.0071434
GAGTACA63200.022.83621
CAACGCA59900.022.8244695
ATCAACG62250.021.7453583
TATCAAC64900.021.4417272
AACGCAG64900.021.4313666
AGTACAT59850.021.425772
CATGGGG63200.021.0431164
GTATAGG5350.018.696651
ACGCAGA75050.018.6597717
ATGGGGG31700.017.6708645
Produced by FastQC (version 0.11.5)