##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921457_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2693392
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.938679924793718	33.0	31.0	34.0	30.0	34.0
2	32.05466378455123	34.0	31.0	34.0	30.0	34.0
3	32.17811703606456	34.0	31.0	34.0	30.0	34.0
4	35.7131665201352	37.0	35.0	37.0	35.0	37.0
5	35.520776775159355	37.0	35.0	37.0	33.0	37.0
6	35.553448959527614	37.0	35.0	37.0	33.0	37.0
7	35.4847749603474	37.0	35.0	37.0	33.0	37.0
8	35.442093464300775	37.0	35.0	37.0	33.0	37.0
9	37.06585450613947	39.0	37.0	39.0	34.0	39.0
10-11	36.97232114746015	39.0	37.0	39.0	33.0	39.0
12-13	36.849341276724665	39.0	37.0	39.0	33.0	39.0
14-15	38.15924306599263	40.0	38.0	41.0	33.0	41.0
16-17	38.061496989669536	40.0	38.0	41.0	33.0	41.0
18-19	37.95111034710135	40.0	38.0	41.0	33.0	41.0
20-21	37.832481309813055	40.0	38.0	41.0	33.0	41.0
22-23	37.79954904447626	40.0	38.0	41.0	33.0	41.0
24-25	37.85705032910174	40.0	38.0	41.0	33.0	41.0
26-27	37.75184915526593	40.0	38.0	41.0	33.0	41.0
28-29	37.6247796458889	40.0	38.0	41.0	32.0	41.0
30-31	37.49813395153769	40.0	38.0	41.0	32.0	41.0
32-33	37.348358501101956	40.0	37.0	41.0	32.0	41.0
34-35	37.20846148648247	40.0	37.0	41.0	31.0	41.0
36-37	37.114317188140454	40.0	37.0	41.0	31.0	41.0
38-39	37.0903370174115	40.0	37.0	41.0	31.0	41.0
40-41	36.936452807463596	40.0	37.0	41.0	31.0	41.0
42-43	36.70900392516202	40.0	36.0	41.0	30.0	41.0
44-45	36.51865584363509	39.0	36.0	41.0	30.0	41.0
46-47	36.269778034537865	39.0	35.5	40.5	30.0	41.0
48-49	35.96026701646103	39.0	35.0	40.0	29.0	41.0
50-51	35.692705703440126	39.0	35.0	40.0	28.0	41.0
52-53	35.385657750524246	38.0	35.0	40.0	28.0	41.0
54-55	35.055913138525696	38.0	34.0	40.0	27.0	41.0
56-57	34.67606831831386	38.0	34.0	40.0	26.0	41.0
58-59	34.28352705436119	37.0	33.5	40.0	26.0	41.0
60-61	33.85978776947433	37.0	33.0	39.5	25.0	41.0
62-63	33.41173082120984	36.0	32.5	39.0	23.5	40.0
64-65	32.89297955886109	35.5	32.0	39.0	22.0	40.0
66-67	32.35399043288166	35.0	31.0	38.0	21.5	40.0
68-69	31.847824044921794	35.0	31.0	37.5	20.0	40.0
70-71	31.283085974859954	34.5	30.5	37.0	18.0	39.0
72-73	30.72130607056084	34.0	30.0	36.0	16.0	39.0
74-75	30.191395088423818	34.0	29.0	36.0	12.0	38.5
76-77	28.909921764080387	32.5	28.0	34.5	10.5	36.0
78-79	29.835707539043703	34.0	29.5	35.0	11.0	37.0
80-81	29.85631408276255	34.0	30.0	35.0	7.5	36.5
82-83	29.50875884386677	34.0	29.5	35.0	6.5	36.0
84-85	29.00831850692361	34.0	29.0	35.0	2.0	36.0
86-87	28.475509320589055	33.5	29.0	35.0	2.0	35.0
88-89	27.919021627746723	33.0	27.0	35.0	2.0	35.0
90-91	27.435031551292944	33.0	26.5	35.0	2.0	35.0
92-93	26.988109974337192	32.5	25.5	34.0	2.0	35.0
94-95	26.476357878838286	32.0	25.0	34.0	2.0	35.0
96-97	25.689079977960876	32.0	23.5	34.0	2.0	35.0
98-99	24.661209916714682	32.0	11.0	34.0	2.0	35.0
100-101	21.054730243499648	27.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	17845.0
3	9380.0
4	5520.0
5	4179.0
6	3600.0
7	3580.0
8	4189.0
9	5105.0
10	5955.0
11	7094.0
12	8134.0
13	9586.0
14	10669.0
15	11863.0
16	13275.0
17	14914.0
18	15595.0
19	15190.0
20	15552.0
21	16483.0
22	17796.0
23	19571.0
24	22389.0
25	26208.0
26	31283.0
27	37632.0
28	44869.0
29	55363.0
30	68313.0
31	84582.0
32	108237.0
33	143276.0
34	203238.0
35	303561.0
36	472095.0
37	587729.0
38	261587.0
39	7954.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.8699694565	17.177469243280225	12.895680968095991	25.056880332123782
2	16.277912758335958	18.47365700945128	38.660618283562144	26.587811948650625
3	20.568381561740456	21.854014479289894	27.78081007683722	29.796793882132434
4	13.8733597848216	14.503551632413474	34.45558973444489	37.16749884832004
5	15.236877083812905	35.686720774640044	33.175786558153696	15.900615583393357
6	35.768112043685655	32.945954310328496	16.2665380687199	15.019395577265954
7	31.924105304929796	29.87822278237942	18.782577096341257	19.415094816349523
8	28.42391455847344	34.07733521155029	18.287415512758802	19.21133471721746
9	26.925419638334507	16.157474146443345	18.432237797347955	38.484868417874196
10-11	27.086054098410617	24.747261519753405	25.948944634273424	22.217739747562554
12-13	26.878229147077775	21.6349602300643	26.528044847375764	24.958765775482156
14-15	23.82624335270778	23.362597797186694	23.908629187025504	28.902529663080017
16-17	24.53420912862454	26.492231866617637	24.552965558737338	24.42059344602048
18-19	24.51605426766454	25.84581744787909	25.839744621881156	23.798383662575215
20-21	24.978450316885258	25.044687042150475	25.835943868692702	24.140918772271565
22-23	24.73108274678459	24.807264902739604	25.645751489052852	24.81590086142295
24-25	24.5537723865907	25.050901406149023	25.76918505753103	24.626141149729243
26-27	24.562907506900288	25.224183918546434	25.75242148340635	24.46048709114693
28-29	24.51117725592286	25.366716863275208	25.71511598758887	24.40698989321306
30-31	24.676476157590475	25.200422528412993	25.631119329004086	24.491981984992446
32-33	24.452520189362296	25.1755499860763	25.614554905782978	24.757374918778428
34-35	24.641797584259443	25.264465295543914	25.76595610377362	24.327781016423025
36-37	24.643392699963442	25.284896886459162	25.553952501621257	24.517757911956135
38-39	24.56262175183013	25.233960321992978	25.725089473437958	24.478328452738936
40-41	24.739800827453237	25.145599016057762	25.636493205503037	24.478106950985968
42-43	24.723025759096366	25.117300294322842	25.61351293449934	24.54616101208146
44-45	24.590747112917203	25.160412055537595	25.59583941471671	24.65300141682849
46-47	24.62666973171767	25.225041714279563	25.68082569205967	24.467462861943094
48-49	24.56869313258031	25.32192538148	25.532724222763974	24.576657263175715
50-51	24.539719440083186	25.375001647655047	25.500613298980422	24.58466561328134
52-53	24.68025676735851	25.36504026795053	25.41930388852771	24.53539907616325
54-55	24.704156924045144	25.14245190107592	25.466685107643013	24.68670606723592
56-57	24.588408512916608	25.384496428260473	25.457641020081162	24.569454038741757
58-59	24.704875956008834	25.371571335273167	25.445271127985535	24.47828158073246
60-61	24.865501692286585	25.203587710681834	25.428056331208825	24.502854265822755
62-63	24.55907095674092	25.233298688046485	25.596127303048227	24.611503052164366
64-65	24.641975097778175	25.252101253762465	25.48028591123578	24.62563773722358
66-67	24.626329880089795	25.437852016158384	25.341244891076485	24.59457321267533
68-69	24.665928847646317	25.31835627593837	25.444093862764923	24.571621013650393
70-71	24.557815652210078	25.178839910569835	25.509171567861138	24.754172869358953
72-73	24.738148750711	25.17315153528592	25.404292609335187	24.68440710466789
74-75	24.554326313005387	25.409845200134324	25.31318815211932	24.722640334740966
76-77	24.644520641682952	25.212505574366944	25.404403447309548	24.73857033664056
78-79	24.631218074568594	25.181390696685924	25.5145967122286	24.672794516516884
80-81	24.554026818280494	25.317438047699596	25.4478395085228	24.68069562549711
82-83	24.696613615211437	25.280601766467132	25.371424560444417	24.651360057877017
84-85	24.64782560580977	25.318956700764474	25.314500442283656	24.718717251142102
86-87	24.587499414536882	25.303678790028023	25.505988823044422	24.602832972390676
88-89	24.602447821480684	25.15891169863687	25.440148221165	24.798492258717445
90-91	24.584276480189875	25.2197144135518	25.398445273874604	24.797563832383727
92-93	24.609729334580436	25.434121093300188	25.511625470913852	24.444524101205527
94-95	24.663533435010194	25.2202560363289	25.368965411610283	24.747245117050614
96-97	24.625449339193146	25.260664536534804	25.435434114152716	24.678452010119333
98-99	24.580010296563685	25.30533692840472	25.434684492754993	24.679968282276597
100-101	25.109279710946463	24.641119766850146	25.56028536276594	24.68931515943745
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	48.0
1	39.0
2	67.5
3	99.0
4	76.0
5	66.5
6	133.0
7	304.0
8	477.0
9	507.0
10	407.5
11	322.0
12	317.0
13	325.0
14	342.0
15	377.0
16	419.5
17	478.0
18	529.5
19	541.0
20	560.0
21	641.5
22	691.0
23	779.0
24	943.0
25	1146.0
26	1478.5
27	1965.0
28	2675.0
29	3863.5
30	5654.0
31	8111.5
32	11568.5
33	15760.0
34	20462.5
35	25976.5
36	32874.0
37	40565.5
38	47596.5
39	54598.0
40	62493.5
41	69784.5
42	77185.5
43	85779.5
44	94827.0
45	103685.5
46	110382.5
47	116493.0
48	124819.0
49	128442.5
50	125606.0
51	123584.5
52	123243.5
53	122325.5
54	120665.0
55	115643.5
56	107387.5
57	98932.0
58	90724.0
59	81925.5
60	71182.5
61	60190.0
62	48676.0
63	36641.0
64	26972.5
65	20176.5
66	15173.0
67	11685.0
68	8987.5
69	6780.0
70	5174.5
71	3934.5
72	2888.0
73	2200.0
74	1711.0
75	1256.5
76	847.5
77	508.5
78	307.0
79	184.0
80	103.0
81	54.0
82	28.0
83	16.5
84	9.5
85	5.5
86	4.0
87	1.0
88	2.5
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3107234297866779
2	0.0
3	0.006163232088013924
4	0.062077855729875196
5	4.4553484973594635E-4
6	0.06679309955624729
7	0.07737455223747601
8	0.10755953830708638
9	0.0951588183227692
10-11	0.0989829924496694
12-13	0.0989829924496694
14-15	0.06640325656272833
16-17	0.03645960187005828
18-19	0.03974542138686088
20-21	0.028848381520402522
22-23	0.04308693275988047
24-25	0.03460320666282517
26-27	0.021144341410385122
28-29	0.02402175398159644
30-31	0.0030444881398623003
32-33	0.005272162388542032
34-35	0.008242394720115006
36-37	0.009968842262841799
38-39	0.015370952315890148
40-41	0.02045747518370887
42-43	0.00337863927716426
44-45	0.015055365130660522
46-47	0.024838567872779008
48-49	0.002450441673547705
50-51	0.006330307656664904
52-53	0.0021905463445350696
54-55	0.004251145024563821
56-57	0.0035828427499599016
58-59	0.0012252208367738524
60-61	0.005290726340614363
62-63	0.014294243095694945
64-65	0.006627330889822202
66-67	0.03876153192702733
68-69	0.06218923944230918
70-71	0.012660615313329808
72-73	0.03269111959937506
74-75	0.05197906580252708
76-77	0.30108873866113806
78-79	0.07291920374011655
80-81	0.03480741013562081
82-83	0.02918253265770449
84-85	0.020420347279564207
86-87	0.1193290839209443
88-89	0.5254526634073318
90-91	0.1334191235438436
92-93	0.047746484730035586
94-95	0.008149574959753351
96-97	0.004882319395023078
98-99	0.009671819029684501
100-101	0.0069986099312688245
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2693392.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.39082441264863
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.07019473254408	43.834105592513204
2	14.623740792032237	17.077845616872825
3	4.669714048541227	8.18005359196948
4	1.9446651357534868	4.542024019327254
5	0.9915882581536649	2.894982793574737
6	0.6094888139296953	2.135313258938538
7	0.40246587200708933	1.6450219845114626
8	0.278671005977419	1.3017463823138904
9	0.2118051561292611	1.1130729913113582
>10	1.10339251682888	11.55885109345731
>50	0.06691075496690399	2.6755126023738227
>100	0.026280410542702307	2.5387416450820064
>500	7.641194777380531E-4	0.28596141838424516
>1k	3.1838311569710526E-4	0.2167670093698886
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.425580828932439E-5
2	0.0	0.0	0.0	0.0	7.425580828932439E-5
3	0.0	0.0	0.0	0.0	7.425580828932439E-5
4	0.0	0.0	0.0	0.0	7.425580828932439E-5
5	0.0	3.7127904144662194E-5	0.0	0.0	7.425580828932439E-5
6	3.7127904144662194E-5	3.7127904144662194E-5	0.0	0.0	7.425580828932439E-5
7	3.7127904144662194E-5	3.7127904144662194E-5	0.0	0.0	7.425580828932439E-5
8	3.7127904144662194E-5	3.7127904144662194E-5	0.0	0.0	7.425580828932439E-5
9	3.7127904144662194E-5	3.7127904144662194E-5	0.0	0.0	1.1138371243398659E-4
10-11	3.7127904144662194E-5	3.7127904144662194E-5	0.0	3.7127904144662194E-5	1.1138371243398659E-4
12-13	3.7127904144662194E-5	7.425580828932439E-5	0.0	3.7127904144662194E-5	3.1558718522962866E-4
14-15	3.7127904144662194E-5	7.425580828932439E-5	0.0	3.7127904144662194E-5	5.197906580252708E-4
16-17	3.7127904144662194E-5	7.425580828932439E-5	0.0	3.7127904144662194E-5	7.611220349655751E-4
18-19	3.7127904144662194E-5	7.425580828932439E-5	0.0	9.281976036165548E-5	7.796859870379061E-4
20-21	3.7127904144662194E-5	7.425580828932439E-5	0.0	1.1138371243398659E-4	8.168138911825683E-4
22-23	3.7127904144662194E-5	7.425580828932439E-5	0.0	1.485116165786488E-4	8.353778432548994E-4
24-25	3.7127904144662194E-5	9.281976036165548E-5	0.0	2.0420347279564207E-4	9.281976036165549E-4
26-27	3.7127904144662194E-5	1.1138371243398659E-4	0.0	3.8984299351895303E-4	9.281976036165549E-4
28-29	3.7127904144662194E-5	1.1138371243398659E-4	0.0	0.0011695289805568592	9.281976036165549E-4
30-31	3.7127904144662194E-5	1.1138371243398659E-4	0.0	0.004158325264202166	9.281976036165549E-4
32-33	3.7127904144662194E-5	1.1138371243398659E-4	0.0	0.009337667892382542	9.65325507761217E-4
34-35	3.7127904144662194E-5	1.299476645063177E-4	0.0	0.015389516267962481	0.0010024534119058794
36-37	3.7127904144662194E-5	1.8563952072331096E-4	0.0	0.025172719010080967	0.0010024534119058794
38-39	3.7127904144662194E-5	2.2276742486797318E-4	0.0	0.042047351443829935	0.0010024534119058794
40-41	1.4851161657864878E-4	2.598953290126354E-4	0.0	0.05934895477524252	0.0010024534119058794
42-43	1.4851161657864878E-4	2.9702323315729755E-4	0.0	0.07627927906520848	0.0010395813160505416
44-45	1.8563952072331096E-4	2.9702323315729755E-4	0.0	0.09404498119842933	0.0010581452681228726
46-47	2.4133137694030428E-4	2.9702323315729755E-4	0.0	0.11201488680444585	0.0010952731722675348
48-49	2.598953290126354E-4	2.9702323315729755E-4	0.0	0.13158129228868282	0.0011880929326291902
50-51	2.598953290126354E-4	2.9702323315729755E-4	0.0	0.15092493034805182	0.0012252208367738524
52-53	2.598953290126354E-4	2.9702323315729755E-4	0.0	0.17049133583228882	0.0012623487409185146
54-55	2.598953290126354E-4	3.3415113730195977E-4	0.0	0.1885726251507393	0.0012623487409185146
56-57	2.598953290126354E-4	3.3415113730195977E-4	0.0	0.2086216933888569	0.0012809126929908456
58-59	2.598953290126354E-4	3.3415113730195977E-4	0.0	0.22870788953111912	0.0012994766450631768
60-61	2.598953290126354E-4	3.3415113730195977E-4	0.0	0.24794014387805413	0.0012994766450631768
62-63	2.598953290126354E-4	3.3415113730195977E-4	0.0	0.2674322935540018	0.0013737324533525013
64-65	2.598953290126354E-4	3.71279041446622E-4	0.0	0.2881125361625786	0.0013737324533525013
66-67	2.598953290126354E-4	3.71279041446622E-4	0.0	0.3118929587672348	0.0014108603574971635
68-69	2.598953290126354E-4	3.71279041446622E-4	0.0	0.3350978988576486	0.001466552213714157
70-71	2.598953290126354E-4	3.8984299351895303E-4	0.0	0.35843278661256883	0.0015593719740758121
72-73	2.598953290126354E-4	4.0840694559128414E-4	0.0	0.3813778313739701	0.0017264475427267922
74-75	2.598953290126354E-4	4.0840694559128414E-4	0.0	0.40333898667553775	0.001874959159305441
76-77	2.598953290126354E-4	4.0840694559128414E-4	0.0	0.42767632784236387	0.001893523111377772
78-79	2.598953290126354E-4	4.0840694559128414E-4	0.0	0.45401857583300165	0.001912087063450103
80-81	2.598953290126354E-4	4.0840694559128414E-4	0.0	0.47789181819801946	0.0020049068238117587
82-83	2.598953290126354E-4	4.0840694559128414E-4	0.0	0.5030645372081004	0.002153418440390407
84-85	2.598953290126354E-4	4.0840694559128414E-4	0.0	0.5281815643619644	0.0021905463445350696
86-87	2.598953290126354E-4	4.0840694559128414E-4	0.0	0.5545980681608915	0.002227674248679732
88-89	2.598953290126354E-4	4.0840694559128414E-4	0.0	0.5817200021385673	0.002264802152824394
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	3525	0.0	32.970764	1
TACCTGG	3595	0.0	31.442343	2
GTATCAA	5095	0.0	27.57885	1
GGTATCA	3630	0.0	27.293234	1
GTACATG	8380	0.0	25.521173	1
ACCTGGG	4455	0.0	24.62778	3
TACATGG	8685	0.0	23.951965	2
TATACCG	180	1.9643267E-7	23.73586	5
ACATGGG	8475	0.0	23.36993	3
TCAACGC	5925	0.0	23.007143	4
GAGTACA	6320	0.0	22.8362	1
CAACGCA	5990	0.0	22.824469	5
ATCAACG	6225	0.0	21.745358	3
TATCAAC	6490	0.0	21.441727	2
AACGCAG	6490	0.0	21.431366	6
AGTACAT	5985	0.0	21.42577	2
CATGGGG	6320	0.0	21.043116	4
GTATAGG	535	0.0	18.69665	1
ACGCAGA	7505	0.0	18.659771	7
ATGGGGG	3170	0.0	17.670864	5
>>END_MODULE