FastQCFastQC Report
Mon 15 Aug 2016
SRR2921456_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921456_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5795871
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT184900.31902021283772536No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT78570.13556202337836712No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT61110.10543712929428554No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT58540.10100293812612461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG77750.032.9174651
TACCTGG81350.030.4898852
GTACATG181200.025.1748811
TACATGG188050.023.8197542
ACCTGGG100400.023.1520943
ACATGGG195450.021.5808333
CATGGGG148100.019.5958774
GTATAGG13350.018.6193491
GTACCCG14550.018.3491941
GAGTACA150800.018.070591
AGTACAT133750.018.030512
TCGCGTA3603.088644E-916.5447719
GTATATA12300.016.4664331
AGAGTAC168850.016.40269310-11
CCTGGGG104700.015.545254
GTGTAGG20400.015.3437221
GTCTTAG21350.015.0921851
GTGTAGC31200.014.9011141
GTATAAG16400.014.5952471
TATAGGG8800.014.5878552
Produced by FastQC (version 0.11.5)