##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921456_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5795871
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.11015755871723	31.0	31.0	34.0	26.0	34.0
2	30.197768549369027	31.0	31.0	34.0	26.0	34.0
3	30.330984247233935	31.0	31.0	34.0	26.0	34.0
4	33.81956913809849	37.0	35.0	37.0	30.0	37.0
5	33.79493815511077	37.0	35.0	37.0	30.0	37.0
6	33.89505563529623	37.0	35.0	37.0	30.0	37.0
7	33.76780263052784	37.0	35.0	37.0	29.0	37.0
8	33.74476605845782	37.0	35.0	37.0	29.0	37.0
9	35.164470534282074	39.0	35.0	39.0	29.0	39.0
10-11	35.02959123831431	39.0	35.0	39.0	28.5	39.0
12-13	34.86243948493678	39.0	35.0	39.0	27.0	39.0
14-15	35.83385413512482	39.5	36.0	41.0	27.0	41.0
16-17	35.68001375461945	39.0	36.0	41.0	27.0	41.0
18-19	35.48335789392138	39.0	36.0	41.0	27.0	41.0
20-21	35.355137044975635	39.0	36.0	40.0	26.0	41.0
22-23	35.38521345626913	39.0	36.0	41.0	26.5	41.0
24-25	35.2394051730965	39.0	35.5	41.0	25.0	41.0
26-27	34.95489418242745	39.0	35.0	40.0	24.0	41.0
28-29	34.70935101902717	39.0	34.5	40.0	23.5	41.0
30-31	34.450470602261504	38.0	34.0	40.0	22.5	41.0
32-33	34.166126885846836	38.0	34.0	40.0	19.5	41.0
34-35	33.73235170347994	38.0	33.0	40.0	16.5	41.0
36-37	33.33625368818595	38.0	33.0	40.0	15.0	41.0
38-39	33.08962915151148	38.0	32.5	40.0	10.5	41.0
40-41	32.81510509809483	38.0	32.0	40.0	9.0	41.0
42-43	32.50616292184557	38.0	31.0	40.0	8.5	41.0
44-45	32.17789862817858	37.0	31.0	40.0	7.5	41.0
46-47	31.878242372889254	37.0	31.0	40.0	6.5	41.0
48-49	31.651051153484953	37.0	30.0	40.0	2.0	41.0
50-51	30.907200315534972	36.0	29.5	38.5	2.0	39.5
52-53	31.43785921046207	36.5	30.5	39.0	2.0	40.0
54-55	31.85816523521659	37.0	31.0	39.5	2.0	40.5
56-57	31.676591145662144	36.5	31.0	40.0	2.0	41.0
58-59	31.324274815640308	36.0	30.5	39.0	2.0	41.0
60-61	30.829595499968857	35.5	30.0	39.0	2.0	40.0
62-63	30.325015860428913	35.0	29.0	39.0	2.0	40.0
64-65	29.760655387257586	35.0	28.5	38.0	2.0	40.0
66-67	29.236016812658526	34.0	28.0	37.5	2.0	40.0
68-69	28.68814040202068	34.0	26.5	37.0	2.0	39.0
70-71	28.135228337552718	34.0	26.0	36.0	2.0	39.0
72-73	27.56515043209209	33.5	26.0	35.5	2.0	38.5
74-75	26.951613226036258	33.0	24.5	35.0	2.0	37.5
76-77	26.35458967254447	32.5	23.5	35.0	2.0	37.0
78-79	25.76615602728218	32.0	20.5	35.0	2.0	36.0
80-81	25.15168522556834	31.5	19.0	34.5	2.0	36.0
82-83	24.496836886121173	31.0	15.0	34.0	2.0	35.0
84-85	23.85115619378002	31.0	7.5	34.0	2.0	35.0
86-87	23.21700103401197	30.0	2.0	34.0	2.0	35.0
88-89	22.52226110622545	30.0	2.0	34.0	2.0	35.0
90-91	21.740015348857835	29.0	2.0	34.0	2.0	35.0
92-93	20.882103052328112	29.0	2.0	34.0	2.0	35.0
94-95	19.94296991772246	27.0	2.0	33.0	2.0	34.0
96-97	19.027639331517214	25.5	2.0	33.0	2.0	34.0
98	18.400998745486227	25.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	214628.0
3	36332.0
4	27673.0
5	23603.0
6	22014.0
7	23255.0
8	26022.0
9	29889.0
10	33250.0
11	36469.0
12	39770.0
13	43722.0
14	37780.0
15	37808.0
16	41421.0
17	46592.0
18	51514.0
19	52832.0
20	56147.0
21	60979.0
22	66616.0
23	73678.0
24	81470.0
25	91708.0
26	103416.0
27	117730.0
28	134253.0
29	156512.0
30	184045.0
31	221991.0
32	272339.0
33	345985.0
34	459811.0
35	625450.0
36	811065.0
37	791107.0
38	305750.0
39	11245.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.544733976480195	16.711204130543535	13.169123680906983	24.574938212069284
2	16.12013071360646	18.656914413866236	38.486909392395084	26.73604548013222
3	20.796678867606825	22.110637777777395	27.015049744308506	30.077633610307274
4	13.91844505734761	14.432459707524698	34.34232761149082	37.30676762363687
5	15.976480203555557	35.363306721231744	32.185651563724974	16.474561511487725
6	35.66128599530335	32.482536038896356	16.45434716327815	15.401830802522145
7	32.01961804398836	29.591268886440908	19.002123435429233	19.3869896341415
8	28.421252994741263	32.61938713050333	18.57844897640809	20.380910898347313
9	27.08974743467582	16.71509776928182	18.860072191102457	37.3350826049399
10-11	27.10090047897965	24.43937707279017	25.693460962304403	22.766261485925774
12-13	27.511467587145123	21.510567194116142	26.19828801084751	24.779677207891226
14-15	24.32075555862716	23.21989721008034	24.04895338215626	28.410393849136245
16-17	24.797617291204528	26.157709507280742	24.443817476361435	24.6008557251533
18-19	24.766836730642346	25.89545122310375	25.456193133383582	23.881518912870323
20-21	25.50003617600776	24.76903043636899	25.262958133739332	24.467975253883914
22-23	25.093912390805418	24.455553373722008	25.352830980838952	25.09770325463362
24-25	25.140855198628575	24.682899912136534	25.34235932306631	24.83388556616858
26-27	25.088140213435995	24.64579436880003	25.63746998173218	24.6285954360318
28-29	24.80552942775306	25.310020982635233	25.283842334776207	24.600607254835495
30-31	25.1086458516395	24.82335446462204	25.110372170089324	24.957627513649136
32-33	24.997600978110565	24.938876719270255	25.152173928051436	24.911348374567744
34-35	25.35428310863741	24.732770413494485	25.284789255820783	24.628157222047324
36-37	25.27878372341051	24.661302221251212	25.380427126237198	24.67948692910108
38-39	25.070342132751257	24.968611515010604	25.29015695977624	24.670889392461905
40-41	25.178466599122235	24.95531666028612	25.169088801669297	24.697127938922346
42-43	25.04138655003037	24.892544141895158	24.9573558955366	25.10871341253787
44-45	25.138945361930848	24.68520994227306	25.0660875502545	25.10975714554159
46-47	25.430399206459995	24.881458892772386	25.05276538112377	24.63537651964384
48-49	25.325300114302834	24.873229970647476	24.966673232606272	24.834796682443418
50-51	24.769379336967816	25.135434659913912	25.31991905092683	24.775266952191437
52-53	25.1508979069809	25.183136784654884	24.85310266438053	24.81286264398369
54-55	24.934986180008643	24.813200217233263	25.18325152384274	25.068562078915363
56-57	25.161890574935597	24.792760552663964	25.10209891699487	24.94324995540557
58-59	25.52302147607599	24.693429903860075	24.951904028270636	24.831644591793296
60-61	25.194162125472317	24.799096209328575	25.338949682378693	24.667791982820418
62-63	24.909078673721954	25.015388420380596	25.198601245052853	24.87693166084459
64-65	24.95306709280694	25.120918805983823	25.062779593839103	24.86323450737014
66-67	25.14943601341554	24.841133411149315	24.90256662610449	25.106863949330656
68-69	24.917671354366163	24.931138781533445	25.084258138579564	25.066931725520824
70-71	25.200288593084448	24.684624340143216	25.085253531312908	25.029833535459435
72-73	25.1942200265682	24.610730178213508	25.229819918104653	24.965229877113646
74-75	24.839314193506407	25.26811003963654	24.984934961053572	24.90764080580348
76-77	25.11948098044422	24.68504092578869	24.919245484714267	25.276232609052823
78-79	25.08191138700856	24.75287941964487	25.167921376159825	24.99728781718675
80-81	25.206856368077613	24.812444420876254	25.02538129564225	24.955317915403885
82-83	25.2418710652484	24.62545788347359	25.1518261736667	24.980844877611315
84-85	25.06190121726643	24.880745940423736	25.070400180756753	24.98695266155308
86-87	25.075383876427626	24.89354767386896	25.0672572155738	24.96381123412962
88-89	24.92011536388327	24.74124588140884	24.960646980624208	25.377991774083686
90-91	25.12818077826402	24.517070350699004	24.982163779223544	25.37258509181343
92-93	25.32898860917852	24.842758767563872	24.99171479737622	24.83653782588139
94-95	25.298898216164044	24.6222878155624	25.034298871960726	25.044515096312825
96-97	24.957012229278856	24.58790443488227	25.349861317054316	25.105222018784563
98	25.18923040266857	24.885671583598672	24.728156475404507	25.196941538328243
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	687.0
1	502.5
2	594.0
3	849.0
4	646.0
5	542.0
6	902.0
7	1857.0
8	2649.0
9	2506.5
10	2058.5
11	1859.5
12	2040.0
13	2386.5
14	2770.5
15	3285.0
16	3722.5
17	3815.0
18	3764.0
19	3641.0
20	3344.0
21	3023.5
22	2804.5
23	2696.5
24	2864.0
25	3230.0
26	3763.0
27	4907.5
28	6671.0
29	9458.0
30	13491.5
31	18814.0
32	26089.5
33	34720.5
34	44710.0
35	56207.5
36	69268.5
37	83260.0
38	97702.0
39	112235.0
40	127882.5
41	144841.5
42	162551.0
43	180676.0
44	198816.5
45	219704.0
46	251382.5
47	283833.5
48	292420.5
49	281895.0
50	274991.0
51	274136.0
52	273968.5
53	274321.5
54	270898.0
55	260977.5
56	245348.0
57	228342.0
58	210366.5
59	189117.0
60	166036.0
61	141051.0
62	115145.0
63	89685.5
64	66956.5
65	50310.5
66	37889.0
67	28683.5
68	22252.0
69	17182.0
70	12946.5
71	9855.0
72	7678.5
73	5714.0
74	4194.0
75	3076.0
76	2120.5
77	1358.0
78	846.0
79	530.0
80	299.5
81	171.5
82	115.5
83	73.0
84	51.0
85	33.5
86	23.5
87	22.5
88	15.5
89	14.0
90	15.0
91	10.5
92	7.0
93	5.5
94	6.0
95	7.5
96	7.0
97	5.5
98	4.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.46489992617157977
2	0.04741306354126929
3	0.05431452839443804
4	0.05245113288408248
5	0.0628723448123673
6	0.06309664242009527
7	0.04190914532086722
8	0.01949663821020171
9	0.012025802506646543
10-11	0.014562090840186058
12-13	0.010766285170943246
14-15	0.014622478657651282
16-17	0.013561378436476588
18-19	0.054331782056570965
20-21	0.08178235851004965
22-23	0.0972071324568818
24-25	0.06780689218238294
26-27	0.08305912950788587
28-29	0.08440491515425377
30-31	0.05515995783895121
32-33	0.031798499310975004
34-35	0.39163224992412704
36-37	0.613134764386578
38-39	0.562659175816715
40-41	0.5565600062527272
42-43	0.5299117250884293
44-45	0.5149441731881197
46-47	0.5056358224674082
48-49	0.49578398138950985
50-51	0.5094920159541163
52-53	0.5899717229731304
54-55	0.5604161997394352
56-57	0.5178945494128493
58-59	0.4961463082943012
60-61	0.5088363767930653
62-63	0.5205774938745187
64-65	0.5393322246130047
66-67	0.5642292590708109
68-69	0.5194646326669452
70-71	0.4692133417048102
72-73	0.45188203809229016
74-75	0.43236814621995556
76-77	0.4471286541746702
78-79	0.4418835408862619
80-81	0.4379238254267564
82-83	0.45767926856895197
84-85	0.4240777615650866
86-87	0.42690736215488573
88-89	0.45378856775797805
90-91	0.43669018858425246
92-93	0.43204032663943004
94-95	0.4423407629327844
96-97	0.43711290330650904
98	0.43127253867451504
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	5795871.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.62699072607246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.35983750078286	39.87694316046738
2	13.742039565738812	14.738884566983923
3	4.9342264905205715	7.938231547424632
4	2.2780165510886072	4.886526898362732
5	1.2406023027570265	3.32648840923476
6	0.7617784426209959	2.451113128665484
7	0.5072477067343295	1.904151764540439
8	0.36336028016734584	1.5588734699805875
9	0.27103381473661736	1.308125508239936
>10	1.4112893566285842	13.600933932076659
>50	0.09419919977290193	3.4685366935190296
>100	0.034689960120968824	3.0177300343405964
>500	9.039879894936478E-4	0.3245262357984308
>1k	6.134152699332797E-4	0.8411581888173248
>5k	1.291400568257175E-4	0.4376501872375077
>10k+	3.2285014206429375E-5	0.32012627431052765
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	18490	0.31902021283772536	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	7857	0.13556202337836712	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	6111	0.10543712929428554	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	5854	0.10100293812612461	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.4507324265843736E-5
2	0.0	0.0	0.0	0.0	3.4507324265843736E-5
3	0.0	0.0	0.0	0.0	3.4507324265843736E-5
4	0.0	1.7253662132921868E-5	0.0	0.0	3.4507324265843736E-5
5	0.0	1.7253662132921868E-5	0.0	0.0	6.901464853168747E-5
6	0.0	1.7253662132921868E-5	0.0	0.0	6.901464853168747E-5
7	0.0	1.7253662132921868E-5	0.0	0.0	6.901464853168747E-5
8	0.0	1.7253662132921868E-5	0.0	0.0	6.901464853168747E-5
9	0.0	1.7253662132921868E-5	0.0	0.0	6.901464853168747E-5
10-11	0.0	3.4507324265843736E-5	0.0	1.7253662132921868E-5	6.901464853168747E-5
12-13	0.0	3.4507324265843736E-5	0.0	3.4507324265843736E-5	1.2940246599691402E-4
14-15	0.0	4.3134155332304674E-5	0.0	3.4507324265843736E-5	1.725366213292187E-4
16-17	0.0	5.1760986398765605E-5	0.0	5.1760986398765605E-5	2.0704394559506242E-4
18-19	0.0	5.1760986398765605E-5	0.0	6.0387817465226535E-5	2.0704394559506242E-4
20-21	0.0	5.1760986398765605E-5	0.0	8.626831066460935E-5	2.0704394559506242E-4
22-23	0.0	6.0387817465226535E-5	0.0	1.5528295919629682E-4	2.4155126986090617E-4
24-25	0.0	6.901464853168747E-5	0.0	2.501781009273671E-4	2.760585941267499E-4
26-27	0.0	6.901464853168747E-5	0.0	3.3644641159197644E-4	3.019390873261327E-4
28-29	0.0	6.901464853168747E-5	0.0	7.764147959814841E-4	3.278195805255155E-4
30-31	0.0	6.901464853168747E-5	0.0	0.0020359321316847805	3.4507324265843734E-4
32-33	0.0	6.901464853168747E-5	0.0	0.004882786383616888	3.96834229057203E-4
34-35	0.0	6.901464853168747E-5	1.7253662132921868E-5	0.00850605543153048	4.313415533230467E-4
36-37	0.0	8.626831066460935E-5	1.7253662132921868E-5	0.012715948991963415	4.3996838438950764E-4
38-39	0.0	1.2077563493045307E-4	1.7253662132921868E-5	0.019962487087790602	4.917293707882732E-4
40-41	0.0	1.3802929706337495E-4	1.7253662132921868E-5	0.028123469276662642	5.003562018547342E-4
42-43	0.0	1.3802929706337495E-4	1.7253662132921868E-5	0.03624131731020239	5.003562018547342E-4
44-45	8.626831066460934E-6	1.3802929706337495E-4	1.7253662132921868E-5	0.045204594788255295	5.17609863987656E-4
46-47	1.7253662132921868E-5	1.5528295919629682E-4	1.7253662132921868E-5	0.05474586994776109	5.348635261205779E-4
48-49	1.7253662132921868E-5	1.6390979026275774E-4	1.7253662132921868E-5	0.06424401095193458	5.607440193199607E-4
50-51	1.7253662132921868E-5	1.7253662132921867E-4	1.7253662132921868E-5	0.0740440910434342	5.952513435858044E-4
52-53	1.7253662132921868E-5	1.7253662132921867E-4	1.7253662132921868E-5	0.08399945409413012	6.383854989181091E-4
54-55	1.7253662132921868E-5	2.0704394559506242E-4	1.7253662132921868E-5	0.09499866370386781	6.55639161051031E-4
56-57	1.7253662132921868E-5	2.242976077279843E-4	1.7253662132921868E-5	0.10706760036584664	6.728928231839529E-4
58-59	1.7253662132921868E-5	2.242976077279843E-4	1.7253662132921868E-5	0.11842051004930923	6.901464853168747E-4
60-61	1.7253662132921868E-5	2.501781009273671E-4	3.4507324265843736E-5	0.13010986614436382	6.987733163833356E-4
62-63	1.7253662132921868E-5	2.5880493199382804E-4	3.4507324265843736E-5	0.1420148930160799	7.160269785162575E-4
64-65	1.7253662132921868E-5	2.5880493199382804E-4	3.4507324265843736E-5	0.15398893453632767	7.419074717156403E-4
66-67	1.7253662132921868E-5	2.760585941267499E-4	3.4507324265843736E-5	0.16617864683323696	7.591611338485622E-4
68-69	1.7253662132921868E-5	2.760585941267499E-4	3.4507324265843736E-5	0.17857540307574132	7.591611338485622E-4
70-71	1.7253662132921868E-5	2.8468542519321084E-4	3.4507324265843736E-5	0.19199875221515456	7.764147959814841E-4
72-73	2.5880493199382802E-5	3.1056591839259364E-4	3.4507324265843736E-5	0.20423159866739615	8.368026134467106E-4
74-75	3.4507324265843736E-5	3.1919274945905454E-4	3.4507324265843736E-5	0.21781023076600567	8.799367687790153E-4
76-77	3.4507324265843736E-5	3.278195805255155E-4	3.4507324265843736E-5	0.23145787751314684	8.799367687790153E-4
78-79	3.4507324265843736E-5	3.278195805255155E-4	3.4507324265843736E-5	0.24682226364251375	8.799367687790153E-4
80-81	3.4507324265843736E-5	3.278195805255155E-4	3.4507324265843736E-5	0.26283366210186526	9.058172619783981E-4
82-83	3.4507324265843736E-5	3.278195805255155E-4	3.4507324265843736E-5	0.2783705848525614	9.834587415765465E-4
84-85	3.4507324265843736E-5	3.3644641159197644E-4	3.4507324265843736E-5	0.29393338809645697	0.0010179660658423903
86	3.4507324265843736E-5	3.4507324265843734E-4	3.4507324265843736E-5	0.30568313200897673	0.0010179660658423903
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	7775	0.0	32.917465	1
TACCTGG	8135	0.0	30.489885	2
GTACATG	18120	0.0	25.174881	1
TACATGG	18805	0.0	23.819754	2
ACCTGGG	10040	0.0	23.152094	3
ACATGGG	19545	0.0	21.580833	3
CATGGGG	14810	0.0	19.595877	4
GTATAGG	1335	0.0	18.619349	1
GTACCCG	1455	0.0	18.349194	1
GAGTACA	15080	0.0	18.07059	1
AGTACAT	13375	0.0	18.03051	2
TCGCGTA	360	3.088644E-9	16.544771	9
GTATATA	1230	0.0	16.466433	1
AGAGTAC	16885	0.0	16.402693	10-11
CCTGGGG	10470	0.0	15.54525	4
GTGTAGG	2040	0.0	15.343722	1
GTCTTAG	2135	0.0	15.092185	1
GTGTAGC	3120	0.0	14.901114	1
GTATAAG	1640	0.0	14.595247	1
TATAGGG	880	0.0	14.587855	2
>>END_MODULE