FastQCFastQC Report
Mon 15 Aug 2016
SRR2921456_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921456_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5795871
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT189030.32614597529862205No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT75920.13098980291314283No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT62280.1074558077638374No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT61210.10560966591561474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG75800.034.4391171
TACCTGG79950.032.0183832
GTACATG178100.025.5969771
TACATGG184000.023.8497182
ACCTGGG101400.023.8405593
GTATAGG12500.022.1033761
ACATGGG193400.021.6595153
CATGGGG148100.019.6262234
GAGTACA152100.018.854191
AGTACAT137350.018.1880532
GTATATA11900.017.6135311
GTATAGC13350.016.7709351
CCTGGGG111300.016.4714764
AGAGTAC177850.016.4560810-11
TATAGGA16250.016.36682
TATAACG3803.927198E-916.2475852
GTATAAC10800.015.4377841
GTACACG14350.014.9383031
CGTATAC2901.8690414E-514.7394443
CCTATAC11600.014.7394443
Produced by FastQC (version 0.11.5)