##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921456_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5795871
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.871740761656014	33.0	31.0	34.0	30.0	34.0
2	31.98130720990857	34.0	31.0	34.0	30.0	34.0
3	32.1079392553768	34.0	31.0	34.0	30.0	34.0
4	35.65257422050974	37.0	35.0	37.0	35.0	37.0
5	35.445580655608104	37.0	35.0	37.0	33.0	37.0
6	35.485320152915754	37.0	35.0	37.0	33.0	37.0
7	35.41337065645526	37.0	35.0	37.0	33.0	37.0
8	35.36797420094408	37.0	35.0	37.0	33.0	37.0
9	36.976890790012405	39.0	37.0	39.0	33.0	39.0
10-11	36.87948170344026	39.0	37.0	39.0	33.0	39.0
12-13	36.75886963322682	39.0	37.0	39.0	33.0	39.0
14-15	38.029848059075164	40.0	38.0	41.0	33.0	41.0
16-17	37.93656294282602	40.0	38.0	41.0	33.0	41.0
18-19	37.827294120245256	40.0	38.0	41.0	33.0	41.0
20-21	37.701849644341635	40.0	38.0	41.0	32.0	41.0
22-23	37.659695669555106	40.0	38.0	41.0	32.5	41.0
24-25	37.70919651248276	40.0	38.0	41.0	32.5	41.0
26-27	37.594119589618195	40.0	38.0	41.0	32.0	41.0
28-29	37.45644701201942	40.0	38.0	41.0	32.0	41.0
30-31	37.32076197003005	40.0	37.0	41.0	31.5	41.0
32-33	37.15445668131675	40.0	37.0	41.0	31.0	41.0
34-35	36.99481027096704	40.0	37.0	41.0	31.0	41.0
36-37	36.89135360328068	40.0	37.0	41.0	31.0	41.0
38-39	36.854251500766665	40.0	37.0	41.0	31.0	41.0
40-41	36.69460862741769	40.0	36.0	41.0	30.0	41.0
42-43	36.44791464475313	39.0	36.0	41.0	30.0	41.0
44-45	36.23174290801158	39.0	35.5	41.0	30.0	41.0
46-47	35.954769956060105	39.0	35.0	40.0	29.0	41.0
48-49	35.610404803695594	39.0	35.0	40.0	28.0	41.0
50-51	35.31183121570511	38.0	35.0	40.0	27.0	41.0
52-53	34.993219655855	38.0	34.0	40.0	26.5	41.0
54-55	34.64695936124183	38.0	34.0	40.0	26.0	41.0
56-57	34.2309454437478	37.5	33.5	40.0	25.5	41.0
58-59	33.8165212786827	37.0	33.0	40.0	24.0	41.0
60-61	33.34837550732237	36.0	32.5	39.0	23.0	40.5
62-63	32.89502587272905	36.0	32.0	39.0	22.0	40.0
64-65	32.35477765119341	35.0	31.0	38.5	20.5	40.0
66-67	31.796178607149812	35.0	31.0	38.0	18.5	40.0
68-69	31.261121926281657	35.0	30.0	37.0	17.5	39.0
70-71	30.67636469134665	34.0	30.0	36.5	12.5	39.0
72-73	30.105612340233247	34.0	29.0	36.0	8.0	38.5
74-75	29.57225505881687	34.0	29.0	35.5	7.0	37.5
76-77	28.343476243691413	32.0	27.0	34.5	7.0	36.0
78-79	29.271237230780326	33.5	29.0	35.0	6.5	36.0
80-81	29.28957131723601	34.0	29.0	35.0	2.0	36.0
82-83	28.9313832209171	34.0	29.0	35.0	2.0	36.0
84-85	28.415664185762587	33.5	28.5	35.0	2.0	35.5
86-87	27.88817841183836	33.0	27.0	35.0	2.0	35.0
88-89	27.320467450017436	33.0	26.0	35.0	2.0	35.0
90-91	26.804544476576513	32.5	25.0	34.0	2.0	35.0
92-93	26.322844573317795	32.0	25.0	34.0	2.0	35.0
94-95	25.769982889543265	32.0	23.5	34.0	2.0	35.0
96-97	24.944669489710865	31.5	19.0	34.0	2.0	35.0
98-99	23.8724516988042	31.0	4.5	34.0	2.0	35.0
100-101	20.294472306233178	25.5	2.0	32.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	42113.0
3	21368.0
4	12748.0
5	9660.0
6	8487.0
7	8793.0
8	10170.0
9	11964.0
10	14286.0
11	17078.0
12	19813.0
13	23057.0
14	25891.0
15	28694.0
16	32109.0
17	37463.0
18	38178.0
19	35870.0
20	37257.0
21	39239.0
22	42196.0
23	46118.0
24	52433.0
25	61131.0
26	72306.0
27	86935.0
28	105927.0
29	128897.0
30	157199.0
31	194754.0
32	248562.0
33	330014.0
34	460889.0
35	681037.0
36	1018170.0
37	1170149.0
38	453101.0
39	11815.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.765865680837955	17.20705518286984	13.568384943958684	24.45869419233352
2	16.26359523874841	19.025285414392417	38.03637106484945	26.674748282009723
3	20.791615211896453	22.349812605997403	27.299685292646437	29.558886889459714
4	14.196535867296003	14.783357482122328	34.22161631098922	36.798490339592455
5	15.987533129359482	35.38449381202832	32.23181933389822	16.39615372471398
6	35.70963912581393	32.714681019593705	16.302336148810795	15.273343705781569
7	32.00704937271668	29.62542567226564	19.04777526403637	19.31974969098132
8	28.405607217728047	32.731280287310845	18.54126604543101	20.3218464495301
9	26.97214169555064	16.69279190397978	18.863635931909407	37.47143046856017
10-11	27.065840507480516	24.493794437163725	25.711687575809623	22.728677479546135
12-13	27.45826618646456	21.552165334868267	26.307877665032347	24.681690813634834
14-15	24.249099312805193	23.25161888898327	24.09459960595583	28.404682192255713
16-17	24.703277270124996	26.22212207818702	24.584841894296343	24.489758757391638
18-19	24.717836970170314	25.903214253351685	25.615359721077546	23.763589055400455
20-21	25.389113284577608	24.74573410411009	25.435270492108835	24.42988211920347
22-23	24.988491502180445	24.45532053345577	25.542332026419057	25.01385593794473
24-25	25.073617427898586	24.66418345472525	25.49905078111752	24.76314833625864
26-27	24.97151702679522	24.649308245997627	25.83869887776568	24.54047584944147
28-29	24.700477896179937	25.303456826087345	25.45987066498877	24.536194612743945
30-31	24.97306192057248	24.823356078360867	25.291470450760123	24.91211155030653
32-33	24.89146660641371	24.894563801485152	25.342561421735045	24.871408170366088
34-35	25.206889611049	24.768267502199798	25.482400201607426	24.54244268514378
36-37	25.118118388437416	24.70127665008123	25.547772354749455	24.632832606731895
38-39	24.933123105255312	24.9849352266515	25.498906294003632	24.583035374089558
40-41	25.02554476663188	24.98778631407957	25.341452551102904	24.645216368185636
42-43	24.901133223757427	24.969770577316407	25.13439324959047	24.994702949335696
44-45	24.983276763799918	24.701770751933473	25.265895788678	25.04905669558861
46-47	25.218742061432092	24.956898753575807	25.24241970311137	24.581939481880735
48-49	25.136288247562316	24.923389488350224	25.186972266136387	24.753349997951073
50-51	24.673604227725633	25.190155426065232	25.407187509732843	24.7290528364763
52-53	25.045369623971308	25.21204411249113	24.925419205318125	24.817167058219432
54-55	24.90429858358798	24.813825244192607	25.222495224850448	25.059380947368965
56-57	25.112049167740746	24.89113424332303	25.125654152151522	24.8711624367847
58-59	25.43442196554232	24.794821426876855	24.968843844815343	24.801912762765483
60-61	25.119207285141755	24.89425125401664	25.391181930634342	24.595359530207258
62-63	24.84117902753852	25.041738229215397	25.245964355422828	24.87111838782326
64-65	24.924700068501544	25.15838177617423	25.122517681852614	24.794400473471615
66-67	25.096669841960797	24.8848186362782	24.93604679160376	25.082464730157245
68-69	24.891973333151487	25.000183428887556	25.0715868998455	25.036256338115457
70-71	25.1082556604883	24.833027577077452	25.09333812581064	24.96537863662361
72-73	25.145577456164098	24.718728019053238	25.237455710185593	24.89823881459707
74-75	24.78588231435517	25.35919320112532	24.95078773713354	24.90413674738597
76-77	25.028680587419274	24.79201977526394	25.00259356092584	25.176706076390946
78-79	24.99557559177	24.867384298487412	25.229348373155887	24.907691736586703
80-81	25.106284087387632	24.96017663718485	25.079618679489307	24.853920595938213
82-83	25.105521824124022	24.783368963182603	25.230722473935156	24.88038673875822
84-85	24.962306716764466	25.079549782485984	25.09279441789094	24.86534908285861
86-87	24.909666348482162	25.063179143403097	25.11314378381142	24.91401072430332
88-89	24.794224595610086	24.902008718295107	25.00576036716498	25.29800631892983
90-91	25.014105939144578	24.75663151900838	25.00003455220856	25.229227989638485
92-93	25.238683867060995	25.086589737521543	25.005156976642823	24.66956941877464
94-95	25.128791489270654	24.869629676154283	25.071798149364305	24.929780685210755
96-97	24.734818413484675	24.859422485189945	25.445630161173465	24.96012894015191
98-99	24.97621567924888	25.040526308387847	24.941152840931103	25.042105171432173
100-101	25.4312622186792	24.281347544530778	25.131802126848225	25.1555881099418
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	273.0
1	240.5
2	435.5
3	650.0
4	465.0
5	324.5
6	634.5
7	1568.0
8	2306.0
9	2113.0
10	1507.0
11	1074.0
12	1004.5
13	1086.5
14	1175.0
15	1244.5
16	1303.5
17	1414.5
18	1530.5
19	1567.5
20	1656.5
21	1843.5
22	1990.0
23	2202.0
24	2513.0
25	2956.5
26	3606.5
27	4523.5
28	6039.5
29	8326.5
30	11935.5
31	16716.5
32	22530.5
33	29946.5
34	38386.5
35	48274.0
36	60296.5
37	72996.0
38	86006.5
39	99880.0
40	115092.0
41	130709.5
42	146711.0
43	163710.0
44	181460.5
45	199095.0
46	217803.5
47	247630.5
48	288558.0
49	298491.5
50	278032.5
51	269075.5
52	269762.5
53	269894.0
54	267406.0
55	259310.5
56	244502.0
57	226076.0
58	207777.0
59	188377.0
60	165884.5
61	141337.0
62	115937.5
63	89395.0
64	66026.5
65	49433.0
66	37010.0
67	28479.0
68	21998.5
69	16765.0
70	12968.0
71	10175.5
72	7704.5
73	5758.5
74	4389.5
75	3231.5
76	2265.0
77	1363.5
78	806.5
79	489.5
80	278.5
81	135.0
82	69.0
83	32.0
84	20.0
85	14.5
86	9.0
87	5.0
88	2.5
89	3.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3104106354333973
2	0.0
3	0.005659201179598373
4	0.05710962165997138
5	5.176098639876561E-4
6	0.062164944664917496
7	0.07160269785162575
8	0.10188287489490364
9	0.08927044787573774
10-11	0.09423087573895278
12-13	0.09304037305178117
14-15	0.06257903255610761
16-17	0.03440380229304621
18-19	0.03702635893725033
20-21	0.026622400671098445
22-23	0.04013201812117626
24-25	0.032721570235086324
26-27	0.020376574978980728
28-29	0.02255053640772888
30-31	0.002967629886862561
32-33	0.0052537401194747094
34-35	0.007755521128748379
36-37	0.010800792495209089
38-39	0.015442027608965072
40-41	0.01970368215579677
42-43	0.00332995679165392
44-45	0.013639019916074737
46-47	0.02372378543276757
48-49	0.002734705448068116
50-51	0.007151642954096114
52-53	0.0024672736850078272
54-55	0.00415813257403417
56-57	0.0034679860887172954
58-59	0.0011387417007728432
60-61	0.005072576667079029
62-63	0.014217017597527618
64-65	0.006573645272643232
66-67	0.037647490774035515
68-69	0.059274956257653076
70-71	0.011689356095054565
72-73	0.030892682048996607
74-75	0.049233324896292556
76-77	0.2956673811408156
78-79	0.07136114658176484
80-81	0.03187614079057315
82-83	0.027122756872953176
84-85	0.018495925806492244
86-87	0.1169280682748115
88-89	0.5207068963405155
90-91	0.12990282219876872
92-93	0.04741306354126929
94-95	0.007884923594745293
96-97	0.005417649909737467
98-99	0.00968793128763563
100-101	0.007608865000618544
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5795871.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.05353097987369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.2108572137018	34.4409045945581
2	15.723198574094132	15.425568167140646
3	5.73785602434832	8.443862947452756
4	2.7069996172114847	5.311515583415592
5	1.4695628270453212	3.604362283166921
6	0.9301736354689538	2.7376980746482844
7	0.6108946791689872	2.097657874903914
8	0.4128010834738263	1.6199480589367017
9	0.3291162535667804	1.4529882906286227
>10	1.7024703242768189	15.121660674746112
>50	0.11745737943911291	3.947980423978123
>100	0.04656624562281849	3.753374881127474
>500	0.0011289123554172043	0.3617503861429141
>1k	7.408397981860516E-4	0.919232085505957
>5k	1.411123424944269E-4	0.434376486324766
>10k+	3.5278085623606726E-5	0.32711918732309675
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	18903	0.32614597529862205	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	7592	0.13098980291314283	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	6228	0.1074558077638374	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	6121	0.10560966591561474	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	1.7253662132921868E-5
3	0.0	0.0	0.0	0.0	1.7253662132921868E-5
4	0.0	0.0	0.0	0.0	1.7253662132921868E-5
5	0.0	0.0	0.0	0.0	1.7253662132921868E-5
6	0.0	0.0	0.0	0.0	1.7253662132921868E-5
7	0.0	0.0	0.0	0.0	1.7253662132921868E-5
8	0.0	0.0	0.0	0.0	1.7253662132921868E-5
9	0.0	0.0	0.0	0.0	3.4507324265843736E-5
10-11	0.0	0.0	0.0	1.7253662132921868E-5	3.4507324265843736E-5
12-13	0.0	0.0	0.0	3.4507324265843736E-5	6.901464853168747E-5
14-15	0.0	8.626831066460934E-6	0.0	4.3134155332304674E-5	6.901464853168747E-5
16-17	0.0	1.7253662132921868E-5	0.0	5.1760986398765605E-5	8.626831066460933E-5
18-19	0.0	1.7253662132921868E-5	0.0	6.0387817465226535E-5	8.626831066460933E-5
20-21	0.0	1.7253662132921868E-5	0.0	9.489514173107027E-5	1.29402465996914E-4
22-23	0.0	1.7253662132921868E-5	0.0	1.984171145286015E-4	1.552829591962968E-4
24-25	0.0	2.5880493199382802E-5	0.0	3.019390873261327E-4	1.811634523956796E-4
26-27	0.0	3.4507324265843736E-5	0.0	3.7095373585782014E-4	2.3292443879444522E-4
28-29	1.7253662132921868E-5	3.4507324265843736E-5	0.0	8.022952891808669E-4	2.5880493199382804E-4
30-31	1.7253662132921868E-5	3.4507324265843736E-5	0.0	0.0020359321316847805	3.019390873261327E-4
32-33	1.7253662132921868E-5	3.4507324265843736E-5	0.0	0.004770637579752896	3.278195805255155E-4
34-35	1.7253662132921868E-5	3.4507324265843736E-5	0.0	0.008471548107264637	3.4507324265843734E-4
36-37	1.7253662132921868E-5	3.4507324265843736E-5	0.0	0.01265556117449819	3.537000737248983E-4
38-39	1.7253662132921868E-5	6.0387817465226535E-5	0.0	0.01984171145286015	3.6232690479135924E-4
40-41	1.7253662132921868E-5	8.626831066460933E-5	0.0	0.02798543997959927	3.6232690479135924E-4
42-43	1.7253662132921868E-5	1.2077563493045307E-4	0.0	0.03606878068887316	3.8820739799074204E-4
44-45	1.7253662132921868E-5	1.3802929706337495E-4	0.0	0.045083819153324836	4.313415533230467E-4
46-47	1.7253662132921868E-5	1.3802929706337495E-4	0.0	0.054564706495365406	4.6584887758889044E-4
48-49	1.7253662132921868E-5	1.3802929706337495E-4	0.0	0.06404559383740598	4.831025397218123E-4
50-51	1.7253662132921868E-5	1.466561281298359E-4	0.0	0.07381979343570622	4.831025397218123E-4
52-53	1.7253662132921868E-5	1.5528295919629682E-4	0.0	0.08348184423014246	5.607440193199607E-4
54-55	1.7253662132921868E-5	1.725366213292187E-4	0.0	0.09408421961082294	6.125050057187263E-4
56-57	1.7253662132921868E-5	1.8979028346214054E-4	0.0	0.1058857245097415	6.211318367851873E-4
58-59	3.4507324265843736E-5	1.8979028346214054E-4	0.0	0.11711785855827364	6.211318367851873E-4
60-61	5.1760986398765605E-5	2.0704394559506242E-4	0.0	0.12848802190386915	6.211318367851873E-4
62-63	5.1760986398765605E-5	2.0704394559506242E-4	0.0	0.14003934870186036	6.728928231839529E-4
64-65	5.1760986398765605E-5	2.0704394559506242E-4	0.0	0.15182359993864597	6.901464853168747E-4
66-67	5.1760986398765605E-5	2.0704394559506242E-4	0.0	0.1637199999792956	7.074001474497966E-4
68-69	5.1760986398765605E-5	2.0704394559506242E-4	0.0	0.17574580248594213	7.246538095827185E-4
70-71	5.1760986398765605E-5	2.1567077666152334E-4	0.0	0.18878094422736463	7.246538095827185E-4
72-73	5.1760986398765605E-5	2.242976077279843E-4	0.0	0.2005997027884161	7.85041627047945E-4
74-75	5.1760986398765605E-5	2.242976077279843E-4	0.0	0.21383326164436717	8.109221202473278E-4
76-77	5.1760986398765605E-5	2.242976077279843E-4	0.0	0.22729111810804623	8.109221202473278E-4
78-79	5.1760986398765605E-5	2.242976077279843E-4	0.0	0.24210338704915965	8.281757823802497E-4
80-81	5.1760986398765605E-5	2.3292443879444522E-4	0.0	0.25758854881345705	8.454294445131716E-4
82-83	5.1760986398765605E-5	2.4155126986090614E-4	0.0	0.2725733543758997	9.316977551777809E-4
84-85	5.1760986398765605E-5	2.501781009273671E-4	0.0	0.2874805184587441	9.316977551777809E-4
86-87	5.1760986398765605E-5	2.5880493199382804E-4	0.0	0.3029052924055763	9.920855726430074E-4
88-89	5.1760986398765605E-5	2.5880493199382804E-4	0.0	0.3199950447482354	0.0010352197279753122
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	7580	0.0	34.439117	1
TACCTGG	7995	0.0	32.018383	2
GTACATG	17810	0.0	25.596977	1
TACATGG	18400	0.0	23.849718	2
ACCTGGG	10140	0.0	23.840559	3
GTATAGG	1250	0.0	22.103376	1
ACATGGG	19340	0.0	21.659515	3
CATGGGG	14810	0.0	19.626223	4
GAGTACA	15210	0.0	18.85419	1
AGTACAT	13735	0.0	18.188053	2
GTATATA	1190	0.0	17.613531	1
GTATAGC	1335	0.0	16.770935	1
CCTGGGG	11130	0.0	16.471476	4
AGAGTAC	17785	0.0	16.45608	10-11
TATAGGA	1625	0.0	16.3668	2
TATAACG	380	3.927198E-9	16.247585	2
GTATAAC	1080	0.0	15.437784	1
GTACACG	1435	0.0	14.938303	1
CGTATAC	290	1.8690414E-5	14.739444	3
CCTATAC	1160	0.0	14.739444	3
>>END_MODULE