FastQCFastQC Report
Mon 15 Aug 2016
SRR2921455_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921455_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5284939
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71090.13451432457403956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA114850.041.7230341
TCAACGC143700.032.32084
GGTATCA65000.031.7972181
ATCAACG148150.031.3502753
TATCAAC149050.031.3455432
CAACGCA148000.031.2888155
AACGCAG159300.029.2907076
GTACCTG61600.026.5279561
GTACATG187550.024.9854341
ACGCAGA188100.024.6769267
TACATGG190700.024.1148072
TACCTGG64800.023.9860252
CGCAGAG196550.023.5194368
ACATGGG194050.022.6352583
GCAGAGT215900.021.0932039
CATGGGG149550.020.2342434
GAGTACA142450.019.2269611
AGAGTAC179950.019.19650310-11
GTGTAGC27200.019.1234251
AGTACAT134850.018.3941572
Produced by FastQC (version 0.11.5)