##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921455_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5284939
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.11382969604758	31.0	31.0	34.0	26.0	34.0
2	30.213003593797392	31.0	31.0	34.0	26.0	34.0
3	30.338592555183702	31.0	31.0	34.0	26.0	34.0
4	33.84087611985682	37.0	35.0	37.0	30.0	37.0
5	33.82174231339283	37.0	35.0	37.0	30.0	37.0
6	33.919868138496966	37.0	35.0	37.0	30.0	37.0
7	33.80002247897279	37.0	35.0	37.0	29.0	37.0
8	33.7779289410909	37.0	35.0	37.0	29.0	37.0
9	35.195013603752095	39.0	35.0	39.0	29.0	39.0
10-11	35.06002604760434	39.0	35.0	39.0	29.0	39.0
12-13	34.888407605083046	38.5	35.0	39.0	27.5	39.0
14-15	35.85499709646601	39.5	36.0	41.0	27.0	41.0
16-17	35.69652592016672	39.0	36.0	41.0	27.0	41.0
18-19	35.51482694502245	39.0	36.0	40.0	27.0	41.0
20-21	35.39786428944592	39.0	36.0	40.0	27.0	41.0
22-23	35.4328299721151	39.0	36.0	41.0	26.5	41.0
24-25	35.286968780528966	39.0	35.5	41.0	25.5	41.0
26-27	35.002874016142854	39.0	35.0	40.0	24.5	41.0
28-29	34.75195267154455	39.0	34.5	40.0	24.0	41.0
30-31	34.47695801219276	38.0	34.0	40.0	22.5	41.0
32-33	34.187469240420754	38.0	34.0	40.0	20.0	41.0
34-35	33.75344010593121	38.0	33.0	40.0	17.0	41.0
36-37	33.35277332434679	38.0	33.0	40.0	15.0	41.0
38-39	33.10596669138471	38.0	32.5	40.0	11.5	41.0
40-41	32.82469305700596	38.0	32.0	40.0	9.0	41.0
42-43	32.51438114990542	37.5	31.0	40.0	8.5	41.0
44-45	32.17425224775537	37.0	31.0	40.0	8.0	41.0
46-47	31.85130273026803	37.0	30.5	40.0	6.0	41.0
48-49	31.607274180458845	37.0	30.0	40.0	2.0	41.0
50-51	30.88704581074635	36.0	29.5	38.5	2.0	39.5
52-53	31.4369067457543	36.5	30.5	39.0	2.0	40.0
54-55	31.86204949574631	37.0	31.0	39.5	2.0	40.5
56-57	31.703347663993853	37.0	31.0	40.0	2.0	41.0
58-59	31.361665669178016	36.0	31.0	39.0	2.0	41.0
60-61	30.89592159909509	36.0	30.0	39.0	2.0	40.0
62-63	30.396247241453494	35.0	29.0	39.0	2.0	40.0
64-65	29.842459771058852	35.0	28.5	38.0	2.0	40.0
66-67	29.34255674474199	34.5	28.0	38.0	2.0	40.0
68-69	28.80654762524222	34.0	26.5	37.0	2.0	39.5
70-71	28.27223048364418	34.0	26.0	36.5	2.0	39.0
72-73	27.696410308614727	33.5	26.0	36.0	2.0	39.0
74-75	27.079453234938	33.0	24.5	35.0	2.0	38.0
76-77	26.467142099464155	32.5	23.5	35.0	2.0	37.0
78-79	25.872739306924828	32.0	21.0	35.0	2.0	36.5
80-81	25.266718215669094	32.0	19.0	35.0	2.0	36.0
82-83	24.602718309520696	31.0	16.0	34.0	2.0	35.5
84-85	23.95056678989105	31.0	7.5	34.0	2.0	35.0
86-87	23.296636252566017	30.5	2.0	34.0	2.0	35.0
88-89	22.606050419881857	30.0	2.0	34.0	2.0	35.0
90-91	21.826512756344016	29.0	2.0	34.0	2.0	35.0
92-93	20.976886204363005	29.0	2.0	34.0	2.0	35.0
94-95	20.034063590894803	27.5	2.0	33.0	2.0	34.5
96-97	19.11781318573403	26.0	2.0	33.0	2.0	34.0
98	18.488753228750607	25.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	189847.0
3	32265.0
4	24836.0
5	20518.0
6	19480.0
7	20203.0
8	23019.0
9	26967.0
10	30231.0
11	33018.0
12	36212.0
13	40008.0
14	35096.0
15	35862.0
16	40094.0
17	46003.0
18	52184.0
19	49691.0
20	51655.0
21	55115.0
22	60915.0
23	66148.0
24	74032.0
25	82842.0
26	93327.0
27	106435.0
28	121532.0
29	141925.0
30	167306.0
31	199783.0
32	246035.0
33	310719.0
34	413815.0
35	559291.0
36	727678.0
37	731312.0
38	306344.0
39	13196.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.67636425580586	17.346284999003778	13.78712453401837	24.190226211172
2	15.730273266085154	18.69612693200947	39.440746156426606	26.13285364547877
3	20.376741570693714	22.51397705736794	27.313527216686513	29.795754155251835
4	13.851246328564553	14.924893894237668	34.835215640154075	36.3886441370437
5	15.708037992467247	35.68803219104991	32.430812796745144	16.173117019737692
6	34.92940076516112	33.10156845533714	16.761898414504056	15.207132364997683
7	31.36648549435206	30.181943394883458	19.03556787601332	19.416003234751162
8	27.952257431056744	33.49953196899678	18.746373374808236	19.801837225138243
9	26.95989023986375	16.73590386525997	19.003775370203908	37.30043052467237
10-11	26.623570724370076	25.126296306003965	25.81305449942625	22.43707847019971
12-13	26.808823566981253	22.19148877184042	26.508340563048204	24.49134709813012
14-15	23.898611343989952	23.669972684100347	24.262571010173538	28.168844961736166
16-17	24.210283185100472	26.81258883894279	24.77565416971273	24.201473806244003
18-19	24.188291926189027	26.035459436699764	26.39155702815176	23.384691608959447
20-21	24.814841537989846	25.17748870035794	26.346634972560246	23.66103478909197
22-23	24.54167488623377	24.900254783693352	26.217775232539957	24.34029509753292
24-25	24.214400839810406	25.190392623483486	26.3146369228765	24.280569613829606
26-27	24.358123853429056	25.278173947938605	26.247803441355693	24.11589875727665
28-29	24.244214189562324	25.431393329055823	26.17572152660098	24.148670954780872
30-31	24.452500122826272	25.300753968708506	26.117721431153722	24.129024477311503
32-33	24.271682300695005	25.321854414444157	26.040802675078844	24.365660609781997
34-35	24.432900666463837	25.36205363151692	26.138712968598032	24.06633273342121
36-37	24.49585301751312	25.321964983660173	25.98693499160588	24.19524700722083
38-39	24.508900135367544	25.305623681270234	26.011093553950904	24.174382629411316
40-41	24.58134036166073	25.28758260108375	25.948001160815803	24.183075876439723
42-43	24.486877177081798	25.220803263576347	25.96047481512492	24.331844744216937
44-45	24.503311195291616	25.320902373832517	25.898997915101123	24.276788515774747
46-47	24.600983222227736	25.360872076690345	25.815546262361366	24.222598438720553
48-49	24.576111950757003	25.38376001227804	25.66554060928814	24.37458742767682
50-51	24.418100490630806	25.548694030365297	25.635742505038834	24.39746297396506
52-53	24.562145420574446	25.608414391956813	25.577490045244346	24.251950142224395
54-55	24.55508439297338	25.357903565976002	25.68802503267293	24.398987008377688
56-57	24.496573727187705	25.528721351148093	25.676050083399094	24.298654838265104
58-59	24.538402340682943	25.433740763220946	25.756878856116632	24.27097803997947
60-61	24.63977190538981	25.298406504652664	25.69430053538134	24.367521054576184
62-63	24.429585635939787	25.50615967141317	25.7576808160151	24.30657387663194
64-65	24.47899572648353	25.319343612528417	25.78171332221812	24.41994733876993
66-67	24.468553602154046	25.490738286045755	25.646100918435145	24.394607193365054
68-69	24.493037336101	25.439375251098745	25.639921352828743	24.42766605997151
70-71	24.425235644330897	25.286209315460006	25.76977560041285	24.518779439796248
72-73	24.538409473889214	25.295374689702715	25.63379440980618	24.532421426601893
74-75	24.384364790511597	25.484004408251533	25.586218673717635	24.545412127519235
76-77	24.52659522750241	25.313945463823433	25.629832192066278	24.52962711660788
78-79	24.52163701806952	25.233302189309853	25.678698789963928	24.566362002656696
80-81	24.44368014966915	25.415206726900657	25.580463561148402	24.560649562281796
82-83	24.58408798014215	25.390874887147547	25.488770646366753	24.536266486343546
84-85	24.610860833514668	25.32146444226316	25.50980770381998	24.557867020402192
86-87	24.552336489893893	25.315175389375387	25.638582289210376	24.493905831520344
88-89	24.5378985143233	25.138784664086693	25.600194662095404	24.7231221594946
90-91	24.557071952162087	25.1753166276599	25.55876042393975	24.70885099623826
92-93	24.546217114656706	25.32549770080677	25.61919612765856	24.50908905687796
94-95	24.60792033381788	25.138328087927043	25.601267070934398	24.65248450732068
96-97	24.637470292084124	25.158850868162997	25.559166460124104	24.64451237962877
98	24.64576173225093	25.197239342917545	25.491642023825523	24.665356901006007
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	781.0
1	573.5
2	555.5
3	753.0
4	646.5
5	589.0
6	976.5
7	1980.5
8	2891.0
9	2958.0
10	2514.5
11	2229.5
12	2353.5
13	2731.0
14	3193.5
15	3625.0
16	3990.5
17	4231.0
18	4356.0
19	4269.0
20	4070.0
21	4054.5
22	4210.5
23	4511.0
24	4971.0
25	5829.5
26	7435.5
27	9576.0
28	12348.5
29	16332.5
30	21403.0
31	28133.5
32	37329.5
33	47424.0
34	58326.5
35	70819.0
36	83786.0
37	96993.5
38	110275.0
39	122045.0
40	134149.5
41	147685.5
42	161838.5
43	178414.0
44	193745.0
45	206284.0
46	219809.0
47	234052.0
48	240989.0
49	239580.0
50	238238.0
51	236242.5
52	234898.5
53	236096.0
54	233014.0
55	222772.5
56	207628.5
57	192975.0
58	178484.0
59	160479.0
60	139589.0
61	116412.5
62	93379.0
63	71242.5
64	52455.0
65	39296.5
66	29841.5
67	22714.5
68	17013.5
69	12753.5
70	9599.5
71	7241.5
72	5469.5
73	3991.0
74	3065.0
75	2234.5
76	1466.0
77	912.0
78	527.5
79	300.5
80	190.5
81	113.5
82	64.5
83	48.0
84	39.0
85	30.0
86	21.0
87	17.0
88	13.5
89	11.5
90	11.5
91	10.5
92	8.0
93	5.0
94	6.5
95	8.0
96	7.5
97	7.0
98	3.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4744614838506178
2	0.04948023051921696
3	0.0580706797183468
4	0.05572438962871663
5	0.06567720081537365
6	0.06503386321015248
7	0.04365234868368396
8	0.020832785392603394
9	0.013037047352864432
10-11	0.01584691895213928
12-13	0.011608459435387996
14-15	0.01583745810500367
16-17	0.015099512028426439
18-19	0.05600821504278478
20-21	0.08442859983814383
22-23	0.1014675855293694
24-25	0.07089958843422792
26-27	0.08552605810587406
28-29	0.08717224550746944
30-31	0.058439652756635416
32-33	0.034153658159536
34-35	0.3994842703009439
36-37	0.6253241522749837
38-39	0.5743869512968834
40-41	0.5679157318561292
42-43	0.5415294291949254
44-45	0.5269597246060929
46-47	0.5170920610436563
48-49	0.5065337556403205
50-51	0.5207912522736782
52-53	0.601643651894563
54-55	0.5715581580033374
56-57	0.5295709184155201
58-59	0.5073946927296606
60-61	0.519807324171575
62-63	0.532163190530676
64-65	0.5502428694068182
66-67	0.576316964112547
68-69	0.5306210724475722
70-71	0.4800528445077607
72-73	0.46281518102668734
74-75	0.44357181795286565
76-77	0.457763088656274
78-79	0.4521811888462667
80-81	0.4490969526800593
82-83	0.46834977660101657
84-85	0.43527465501493956
86-87	0.4379709964485872
88-89	0.46436675995692667
90-91	0.44792380763524425
92-93	0.44313661898462786
94-95	0.4538652196364045
96-97	0.44872797964177075
98	0.44348667032864525
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	5284939.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.55600090058484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.92473093945944	39.05556955868597
2	14.566253401775937	15.60220560607318
3	5.318713803313662	8.545471237206586
4	2.4071765751539034	5.156750033072368
5	1.2910598023729676	3.4571999969297766
6	0.7830268978770193	2.516147354873028
7	0.5433386110624112	2.036933020036671
8	0.36073688136145365	1.5455699794454543
9	0.27933442232699457	1.3464031116337982
>10	1.4008989455407381	13.371908527829273
>50	0.08744958003198455	3.2070734135406926
>100	0.03582637953300755	3.2806507506106413
>500	0.0010991862078014664	0.3960863037783621
>1k	3.191165844996372E-4	0.34703429197288693
>5k	3.545739827501923E-5	0.13499681431125754
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7109	0.13451432457403956	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.892169427121108E-5
2	0.0	0.0	0.0	0.0	1.892169427121108E-5
3	0.0	0.0	0.0	0.0	1.892169427121108E-5
4	0.0	0.0	0.0	1.892169427121108E-5	1.892169427121108E-5
5	0.0	0.0	0.0	1.892169427121108E-5	1.892169427121108E-5
6	0.0	0.0	0.0	1.892169427121108E-5	3.784338854242216E-5
7	0.0	0.0	0.0	3.784338854242216E-5	5.676508281363323E-5
8	0.0	0.0	0.0	3.784338854242216E-5	5.676508281363323E-5
9	0.0	0.0	0.0	7.568677708484431E-5	5.676508281363323E-5
10-11	1.892169427121108E-5	0.0	0.0	7.568677708484431E-5	9.460847135605538E-5
12-13	3.784338854242216E-5	0.0	0.0	1.3245185989847755E-4	1.7029524844089968E-4
14-15	6.622592994923877E-5	0.0	0.0	1.3245185989847755E-4	2.0813863698332185E-4
16-17	9.460847135605538E-5	0.0	0.0	2.45982025525744E-4	2.2706033125453293E-4
18-19	9.460847135605538E-5	0.0	0.0	4.351989682378548E-4	2.2706033125453293E-4
20-21	1.5137355416968863E-4	0.0	0.0	4.91964051051488E-4	2.3652117839013847E-4
22-23	1.5137355416968863E-4	0.0	0.0	5.487291338651212E-4	2.45982025525744E-4
24-25	1.986777898477163E-4	0.0	0.0	5.771116752719379E-4	2.45982025525744E-4
26-27	2.0813863698332185E-4	0.0	0.0	6.622592994923877E-4	2.45982025525744E-4
28-29	2.0813863698332185E-4	0.0	0.0	9.177021721537373E-4	2.45982025525744E-4
30-31	2.45982025525744E-4	0.0	0.0	0.001702952484408997	2.45982025525744E-4
32-33	2.45982025525744E-4	0.0	0.0	0.003509974287309655	2.45982025525744E-4
34-35	2.554428726613496E-4	0.0	0.0	0.0056765082813633235	2.649037197969551E-4
36-37	2.649037197969551E-4	0.0	0.0	0.0093946212056563	2.649037197969551E-4
38-39	2.649037197969551E-4	0.0	0.0	0.015279268124002945	2.9328626120377174E-4
40-41	2.9328626120377174E-4	0.0	0.0	0.022109999755910145	3.0274710833937726E-4
42-43	3.0274710833937726E-4	0.0	0.0	0.02871367105656281	3.1220795547498277E-4
44-45	3.4059049688179937E-4	0.0	0.0	0.03579984556113136	3.2166880261058834E-4
46-47	3.7843388542422153E-4	0.0	0.0	0.043661809530819565	3.2166880261058834E-4
48-49	3.7843388542422153E-4	0.0	0.0	0.05111695707367672	3.2166880261058834E-4
50-51	3.7843388542422153E-4	0.0	0.0	0.05878024325351722	3.4059049688179937E-4
52-53	3.973555796954326E-4	0.0	0.0	0.06633946011486605	4.162772739666437E-4
54-55	3.973555796954326E-4	1.892169427121108E-5	0.0	0.07457039712284286	4.7304235678027694E-4
56-57	3.973555796954326E-4	1.892169427121108E-5	0.0	0.08355820190166811	4.7304235678027694E-4
58-59	3.973555796954326E-4	1.892169427121108E-5	0.0	0.09323664852139259	5.014248981870936E-4
60-61	3.973555796954326E-4	1.892169427121108E-5	0.0	0.10259342633850646	5.10885745322699E-4
62-63	3.973555796954326E-4	2.8382541406816616E-5	0.0	0.112054273474112	5.487291338651212E-4
64-65	3.973555796954326E-4	3.784338854242216E-5	0.0	0.12160026823393799	5.581899810007268E-4
66-67	3.973555796954326E-4	3.784338854242216E-5	9.46084713560554E-6	0.1325559292169692	5.771116752719379E-4
68-69	4.162772739666437E-4	3.784338854242216E-5	1.892169427121108E-5	0.1433318341044239	5.865725224075434E-4
70-71	4.446598153734603E-4	5.676508281363323E-5	1.892169427121108E-5	0.1540793564504718	5.865725224075434E-4
72-73	4.5412066250906586E-4	7.568677708484431E-5	1.892169427121108E-5	0.16514854759913028	6.244159109499655E-4
74-75	4.5412066250906586E-4	7.568677708484431E-5	1.892169427121108E-5	0.17716382346134932	6.717201466279932E-4
76-77	4.5412066250906586E-4	7.568677708484431E-5	3.784338854242216E-5	0.1901157231899933	7.001026880348099E-4
78-79	4.7304235678027694E-4	7.568677708484431E-5	3.784338854242216E-5	0.20368257798245165	7.001026880348099E-4
80-81	4.7304235678027694E-4	7.568677708484431E-5	3.784338854242216E-5	0.21662501686396002	7.001026880348099E-4
82-83	4.91964051051488E-4	7.568677708484431E-5	4.730423567802769E-5	0.23048515791762214	7.474069237128375E-4
84-85	5.10885745322699E-4	7.568677708484431E-5	5.676508281363323E-5	0.2447804979395221	8.136328536620764E-4
86	5.10885745322699E-4	7.568677708484431E-5	5.676508281363323E-5	0.25578346315823136	8.136328536620764E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	11485	0.0	41.723034	1
TCAACGC	14370	0.0	32.3208	4
GGTATCA	6500	0.0	31.797218	1
ATCAACG	14815	0.0	31.350275	3
TATCAAC	14905	0.0	31.345543	2
CAACGCA	14800	0.0	31.288815	5
AACGCAG	15930	0.0	29.290707	6
GTACCTG	6160	0.0	26.527956	1
GTACATG	18755	0.0	24.985434	1
ACGCAGA	18810	0.0	24.676926	7
TACATGG	19070	0.0	24.114807	2
TACCTGG	6480	0.0	23.986025	2
CGCAGAG	19655	0.0	23.519436	8
ACATGGG	19405	0.0	22.635258	3
GCAGAGT	21590	0.0	21.093203	9
CATGGGG	14955	0.0	20.234243	4
GAGTACA	14245	0.0	19.226961	1
AGAGTAC	17995	0.0	19.196503	10-11
GTGTAGC	2720	0.0	19.123425	1
AGTACAT	13485	0.0	18.394157	2
>>END_MODULE