FastQCFastQC Report
Mon 15 Aug 2016
SRR2921455_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921455_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5284939
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67930.12853506918433683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA118250.038.585181
GGTATCA71450.034.062371
TCAACGC146100.030.8858174
ATCAACG150700.030.0058083
CAACGCA151100.029.9127665
TATCAAC151900.029.7994692
AACGCAG161350.028.0141036
GTACCTG57400.027.713461
TACCTGG63800.024.7167172
ACGCAGA190100.023.7775847
GTACATG190800.023.364351
TACATGG192450.022.6567782
CGCAGAG199850.022.570048
ACATGGG191850.021.7872523
GAGTACA144950.020.273241
GCAGAGT221800.020.121619
AGTACAT140250.019.3039132
CATGGGG142100.019.019714
AGAGTAC183400.018.83319310-11
ACCTGGG83050.018.7020723
Produced by FastQC (version 0.11.5)