##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921455_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5284939
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90652645186633	33.0	31.0	34.0	30.0	34.0
2	32.02253138588733	34.0	31.0	34.0	30.0	34.0
3	32.14585031161192	34.0	31.0	34.0	30.0	34.0
4	35.69091298877811	37.0	35.0	37.0	35.0	37.0
5	35.49204049469635	37.0	35.0	37.0	33.0	37.0
6	35.52959192149616	37.0	35.0	37.0	33.0	37.0
7	35.46394575225939	37.0	35.0	37.0	33.0	37.0
8	35.4198379583946	37.0	35.0	37.0	33.0	37.0
9	37.03572586173653	39.0	37.0	39.0	34.0	39.0
10-11	36.944992837192636	39.0	37.0	39.0	33.0	39.0
12-13	36.82483932170267	39.0	37.0	39.0	33.0	39.0
14-15	38.114916463558046	40.0	38.0	41.0	33.0	41.0
16-17	38.020697022236206	40.0	38.0	41.0	33.0	41.0
18-19	37.922620866579535	40.0	38.0	41.0	33.0	41.0
20-21	37.811193468836635	40.0	38.0	41.0	33.0	41.0
22-23	37.774672990549185	40.0	38.0	41.0	32.5	41.0
24-25	37.82953228031582	40.0	38.0	41.0	33.0	41.0
26-27	37.718884077943	40.0	38.0	41.0	32.5	41.0
28-29	37.585711869143616	40.0	38.0	41.0	32.0	41.0
30-31	37.44259347175057	40.0	37.5	41.0	32.0	41.0
32-33	37.27812298306565	40.0	37.0	41.0	31.5	41.0
34-35	37.13031437070513	40.0	37.0	41.0	31.0	41.0
36-37	37.02880004859091	40.0	37.0	41.0	31.0	41.0
38-39	36.9932851637455	40.0	37.0	41.0	31.0	41.0
40-41	36.8273960210326	40.0	36.5	41.0	30.5	41.0
42-43	36.587293533567745	39.5	36.0	41.0	30.0	41.0
44-45	36.37554283975652	39.0	36.0	41.0	30.0	41.0
46-47	36.091388188208036	39.0	35.0	40.5	29.5	41.0
48-49	35.75072966783533	39.0	35.0	40.0	28.5	41.0
50-51	35.469857551809014	38.5	35.0	40.0	28.0	41.0
52-53	35.15603841406684	38.0	34.5	40.0	27.0	41.0
54-55	34.82458709551804	38.0	34.0	40.0	26.0	41.0
56-57	34.43889569964762	38.0	34.0	40.0	26.0	41.0
58-59	34.04531149744585	37.0	33.0	40.0	25.0	41.0
60-61	33.61478070040165	36.5	33.0	39.0	23.5	41.0
62-63	33.173507868302735	36.0	32.5	39.0	22.5	40.0
64-65	32.65105131771625	35.5	31.5	39.0	21.5	40.0
66-67	32.116075228115214	35.0	31.0	38.0	20.0	40.0
68-69	31.60507898009797	35.0	31.0	37.5	18.0	39.5
70-71	31.047591561605536	34.5	30.0	37.0	15.5	39.0
72-73	30.486254808239035	34.0	29.5	36.0	12.0	39.0
74-75	29.956395901636707	34.0	29.0	36.0	8.0	38.5
76-77	28.690314022545955	32.5	27.5	34.5	7.5	36.0
78-79	29.618543752349836	34.0	29.0	35.0	7.0	37.0
80-81	29.640703232336268	34.0	30.0	35.0	7.0	36.5
82-83	29.290750375737545	34.0	29.0	35.0	2.0	36.0
84-85	28.77889943479007	34.0	29.0	35.0	2.0	36.0
86-87	28.23704672844852	33.0	27.5	35.0	2.0	35.0
88-89	27.680346357829297	33.0	27.0	35.0	2.0	35.0
90-91	27.185686249169574	33.0	26.0	34.5	2.0	35.0
92-93	26.724582913823603	32.0	25.0	34.0	2.0	35.0
94-95	26.199521697412216	32.0	24.5	34.0	2.0	35.0
96-97	25.39981464308292	32.0	21.5	34.0	2.0	35.0
98-99	24.350494963139596	31.5	10.0	34.0	2.0	35.0
100-101	20.759367894312497	27.0	2.0	32.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	35817.0
3	18166.0
4	11067.0
5	7967.0
6	6862.0
7	7348.0
8	8530.0
9	10118.0
10	12160.0
11	14375.0
12	16938.0
13	20181.0
14	22544.0
15	25973.0
16	29605.0
17	35893.0
18	35929.0
19	31238.0
20	31495.0
21	33166.0
22	35758.0
23	39221.0
24	44536.0
25	52181.0
26	62117.0
27	74965.0
28	91154.0
29	110455.0
30	135711.0
31	168455.0
32	217373.0
33	288216.0
34	406798.0
35	603314.0
36	923299.0
37	1117469.0
38	483559.0
39	14986.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.89890575699488	17.79030827438586	14.180097870488353	24.13068809813091
2	15.877193662973216	18.995205053454733	39.06012917083811	26.06747211273394
3	20.425609377870956	22.685454047611074	27.52878121019693	29.360155364321045
4	14.05750792673195	15.220234120456034	34.804804639923695	35.91745331288833
5	15.747798921835946	35.733380511683265	32.48403473285259	16.0347858336282
6	34.977781993730225	33.250270234347	16.593572275593782	15.178375496328997
7	31.33965697228439	30.21021842136507	19.109582958327646	19.340541648022896
8	28.04505094578423	33.54913520776645	18.695854596257927	19.7099592501914
9	26.885831576443458	16.788323241475076	19.01870850981144	37.30713667227003
10-11	26.59850616220166	25.2045946786207	25.863178412506848	22.33372074667079
12-13	26.800213752611185	22.16899240530495	26.623383940959126	24.407409901124737
14-15	23.844897884221545	23.665576493845542	24.33220485125801	28.157320770674904
16-17	24.159357195585926	26.835588953455357	24.882313414472563	24.122740436486154
18-19	24.128282197893554	26.088396763084738	26.50424546525404	23.279075573767667
20-21	24.711264621306714	25.18908497841665	26.46326628839261	23.636384111884023
22-23	24.490905996800542	24.892721939089874	26.3387160554308	24.277656008678786
24-25	24.147389150179137	25.186648808748664	26.435886011162967	24.230076029909238
26-27	24.248336202140678	25.315409517122472	26.372480646196095	24.063773634540755
28-29	24.18899450535308	25.47112925668586	26.310088710764145	24.029787527196913
30-31	24.3789334216628	25.345647233332	26.183159328253254	24.092260016751947
32-33	24.149797858138328	25.32154889959704	26.18679932748685	24.341853914777786
34-35	24.343351396521662	25.361554711515527	26.2877815696817	24.007312322281116
36-37	24.368912628162605	25.35837178885621	26.13282821931887	24.139887363662314
38-39	24.36328020175189	25.37424438609765	26.16604221634885	24.096433195801605
40-41	24.465366984479118	25.32102584056815	26.105856083260004	24.107751091692727
42-43	24.38962061752826	25.249633568008754	26.11026149601135	24.250484318451633
44-45	24.38190422607284	25.274456381348603	26.04536225441521	24.29827713816334
46-47	24.427739167407083	25.372241962977508	25.995355652031797	24.204663217583605
48-49	24.423726082665702	25.425165779955556	25.832078711146885	24.319029426231857
50-51	24.3998817309853	25.565806062469694	25.690556219176138	24.34375598736887
52-53	24.506262048085905	25.574568634175765	25.692094090376905	24.227075227361425
54-55	24.50016022613125	25.337754508589246	25.76496742447114	24.39711784080836
56-57	24.47376575112222	25.554906412185645	25.72080853513676	24.25051930155537
58-59	24.538503486934026	25.471194004320257	25.753338964500937	24.23696354424478
60-61	24.65764804202506	25.389067387823598	25.696627585917835	24.25665698423351
62-63	24.393426670576456	25.4821876996233	25.852501191530212	24.271884438270032
64-65	24.466976701235218	25.34770081664135	25.78750937635063	24.397813105772805
66-67	24.4275214473308	25.586859903308483	25.6327250070913	24.352893642269414
68-69	24.490616803447264	25.487169938101072	25.66867209207242	24.353541166379248
70-71	24.44248854055212	25.36143009670559	25.765571734762275	24.430509627980015
72-73	24.58027914102388	25.362999074337573	25.616309375102624	24.440412409535924
74-75	24.410752165019495	25.539288395079023	25.56058599640118	24.489373443500302
76-77	24.49646555844632	25.338937349291722	25.7409241483117	24.423672943950255
78-79	24.43356796299198	25.299641593689092	25.781205989186745	24.48558445413218
80-81	24.41909524225943	25.481948417777467	25.64414368884553	24.454812651117575
82-83	24.481968611545565	25.51397626489335	25.571363124343527	24.432691999217553
84-85	24.511905481747867	25.497836394951108	25.58296308051105	24.407295042789976
86-87	24.410373248102616	25.51278233432749	25.693503582599842	24.383340834970056
88-89	24.46892515398453	25.315232592365756	25.626953486282027	24.588888767367692
90-91	24.47926263090252	25.36792243975668	25.65020116551046	24.502613763830336
92-93	24.505473837471527	25.568636107251034	25.657441203539065	24.268448851738366
94-95	24.49251700062371	25.419877937641168	25.595561141691043	24.492043920044086
96-97	24.45725556099164	25.458987654141108	25.585826790874282	24.497929993992976
98-99	24.491164984782092	25.416218058456785	25.602417076907223	24.4901998798539
100-101	24.934559122542527	24.85768395022387	25.75088356774978	24.456873359483822
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	344.0
1	262.5
2	396.0
3	610.5
4	461.0
5	335.0
6	655.5
7	1644.0
8	2441.5
9	2332.0
10	1736.0
11	1199.0
12	1093.0
13	1187.5
14	1261.5
15	1382.5
16	1549.5
17	1682.0
18	1918.5
19	2136.0
20	2277.5
21	2547.5
22	2925.0
23	3436.0
24	4002.0
25	4796.5
26	6000.0
27	7697.0
28	9978.0
29	13383.0
30	18132.0
31	23637.5
32	31284.0
33	40761.5
34	50995.0
35	62110.5
36	74856.5
37	87954.0
38	99726.5
39	112240.0
40	125349.5
41	137436.5
42	150176.5
43	165736.5
44	182323.0
45	198736.0
46	209798.0
47	219460.5
48	233800.0
49	240891.0
50	237231.0
51	233158.0
52	232695.5
53	231644.5
54	228718.5
55	220122.0
56	205576.5
57	189369.0
58	174556.5
59	158535.5
60	137653.5
61	115956.5
62	93718.5
63	70878.5
64	52174.0
65	38910.5
66	29129.0
67	22520.0
68	17258.0
69	12957.5
70	9766.5
71	7315.0
72	5403.0
73	4032.5
74	3055.5
75	2201.5
76	1504.0
77	880.5
78	506.0
79	273.0
80	151.5
81	85.5
82	42.0
83	19.5
84	11.5
85	10.0
86	6.5
87	2.0
88	2.5
89	2.0
90	1.0
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3131161968000009
2	0.0
3	0.006206315720957234
4	0.05918705968034825
5	5.487291338651212E-4
6	0.0645608208533722
7	0.07394598121189289
8	0.10227175753589586
9	0.0903510901450329
10-11	0.09552617352820914
12-13	0.09467469728600462
14-15	0.06358635359840482
16-17	0.03479699576475717
18-19	0.03799476209659185
20-21	0.027777047190137863
22-23	0.04109791995707046
24-25	0.03320757344597544
26-27	0.020700333532704916
28-29	0.023671039533285056
30-31	0.0033964441216823883
32-33	0.005373761173023946
34-35	0.008221476160841214
36-37	0.011116495384336508
38-39	0.01561039777374914
40-41	0.020009691691805713
42-43	0.003651886994343738
44-45	0.014494017811747686
46-47	0.024834723730964542
48-49	0.0027909499050036337
50-51	0.0071240178931109704
52-53	0.002393594325308201
54-55	0.004172233586802043
56-57	0.0037181129242929768
58-59	0.0012677535161711422
60-61	0.005345378631617129
62-63	0.014522400353154502
64-65	0.006944261797534466
66-67	0.03889354257447437
68-69	0.06023721371240046
70-71	0.012355866359100832
72-73	0.03208173263683838
74-75	0.0502181765957942
76-77	0.3001075319885433
78-79	0.07420142408455424
80-81	0.03407797138245115
82-83	0.028373080559681012
84-85	0.019858318137636024
86-87	0.11525203980594667
88-89	0.5205641919424236
90-91	0.12909325916533756
92-93	0.04890311884394503
94-95	0.00807956345380713
96-97	0.00553459557432924
98-99	0.010208254059318377
100-101	0.007530834319942009
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5284939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.549174704811946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.3025029956258	33.160301507105785
2	16.525111168837125	16.045610183046254
3	6.3135785267790085	9.195570807274299
4	2.9315362949367003	5.69294670945516
5	1.607454173866615	3.9020286758514726
6	0.9753678533506661	2.8411982588267333
7	0.6652607627892773	2.2608502697839157
8	0.457251978144994	1.7759364936865734
9	0.3306327539443106	1.4446752600937893
>10	1.731180553269485	15.090168158257097
>50	0.1112143424724485	3.684959681467691
>100	0.04695507509531808	3.89644948399209
>500	0.0014846779431102928	0.4774338921035442
>1k	4.2977269963794613E-4	0.403019081425277
>5k	3.9070245419351146E-5	0.12885153763048546
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6793	0.12853506918433683	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.892169427121108E-5
2	0.0	0.0	0.0	0.0	1.892169427121108E-5
3	0.0	0.0	0.0	0.0	1.892169427121108E-5
4	0.0	0.0	0.0	1.892169427121108E-5	1.892169427121108E-5
5	1.892169427121108E-5	0.0	0.0	1.892169427121108E-5	1.892169427121108E-5
6	1.892169427121108E-5	0.0	0.0	1.892169427121108E-5	1.892169427121108E-5
7	1.892169427121108E-5	0.0	0.0	1.892169427121108E-5	1.892169427121108E-5
8	1.892169427121108E-5	0.0	0.0	1.892169427121108E-5	1.892169427121108E-5
9	1.892169427121108E-5	0.0	0.0	3.784338854242216E-5	1.892169427121108E-5
10-11	3.784338854242216E-5	0.0	0.0	8.514762422044984E-5	5.676508281363323E-5
12-13	5.676508281363323E-5	0.0	0.0	1.1353016562726646E-4	1.0406931849166092E-4
14-15	6.622592994923877E-5	0.0	0.0	1.1353016562726646E-4	1.7029524844089968E-4
16-17	7.568677708484431E-5	0.0	0.0	2.0813863698332185E-4	1.8921694271211077E-4
18-19	7.568677708484431E-5	0.0	0.0	3.5951219115301045E-4	1.986777898477163E-4
20-21	7.568677708484431E-5	0.0	0.0	4.162772739666437E-4	2.0813863698332185E-4
22-23	7.568677708484431E-5	1.892169427121108E-5	0.0	5.392682867295156E-4	2.3652117839013847E-4
24-25	7.568677708484431E-5	3.784338854242216E-5	0.0	5.96033369543149E-4	2.45982025525744E-4
26-27	7.568677708484431E-5	3.784338854242216E-5	0.0	6.717201466279933E-4	2.45982025525744E-4
28-29	7.568677708484431E-5	6.622592994923877E-5	0.0	9.271630192893429E-4	2.45982025525744E-4
30-31	7.568677708484431E-5	9.460847135605538E-5	0.0	0.0017218741786802082	2.45982025525744E-4
32-33	9.460847135605538E-5	9.460847135605538E-5	0.0	0.0035005134401740494	2.45982025525744E-4
34-35	9.460847135605538E-5	9.460847135605538E-5	0.0	0.005723812517041351	2.649037197969551E-4
36-37	9.460847135605538E-5	9.460847135605538E-5	0.0	0.009451386288469933	2.649037197969551E-4
38-39	9.460847135605538E-5	9.460847135605538E-5	0.0	0.015402259136765819	2.649037197969551E-4
40-41	9.460847135605538E-5	9.460847135605538E-5	0.0	0.0222046082272662	2.649037197969551E-4
42-43	9.460847135605538E-5	1.0406931849166092E-4	0.0	0.02877989698651205	2.649037197969551E-4
44-45	1.1353016562726646E-4	1.1353016562726646E-4	0.0	0.03571469793691091	2.743645669325606E-4
46-47	1.3245185989847755E-4	1.1353016562726646E-4	0.0	0.04344421004670063	2.838254140681662E-4
48-49	1.3245185989847755E-4	1.1353016562726646E-4	0.0	0.05086151420101538	3.0274710833937726E-4
50-51	1.3245185989847755E-4	1.1353016562726646E-4	0.0	0.05830720089673694	3.2166880261058834E-4
52-53	1.3245185989847755E-4	1.1353016562726646E-4	0.0	0.06569612250964486	4.5412066250906586E-4
54-55	1.3245185989847755E-4	1.1353016562726646E-4	0.0	0.07377568596345199	5.10885745322699E-4
56-57	1.3245185989847755E-4	1.1353016562726646E-4	0.0	0.08252696956388711	5.203465924583046E-4
58-59	1.3245185989847755E-4	1.1353016562726646E-4	0.0	0.09198781669949266	5.298074395939102E-4
60-61	1.7029524844089968E-4	1.1353016562726646E-4	0.0	0.1009472389369111	5.298074395939102E-4
62-63	1.7029524844089968E-4	1.1353016562726646E-4	0.0	0.11019048658839771	5.298074395939102E-4
64-65	1.7029524844089968E-4	1.1353016562726646E-4	0.0	0.11944319508701992	5.298074395939102E-4
66-67	1.7029524844089968E-4	1.1353016562726646E-4	0.0	0.12990689201899963	5.298074395939102E-4
68-69	2.0813863698332185E-4	1.1353016562726646E-4	0.0	0.1402759804796233	5.298074395939102E-4
70-71	2.0813863698332185E-4	1.1353016562726646E-4	0.0	0.15062614724597578	5.298074395939102E-4
72-73	2.0813863698332185E-4	1.1353016562726646E-4	0.0	0.16130744366207445	5.298074395939102E-4
74-75	2.0813863698332185E-4	1.1353016562726646E-4	0.0	0.1728969814031912	5.298074395939102E-4
76-77	2.0813863698332185E-4	1.1353016562726646E-4	0.0	0.18536637792791932	5.487291338651212E-4
78-79	2.0813863698332185E-4	1.1353016562726646E-4	0.0	0.19820474749093603	5.581899810007268E-4
80-81	2.175994841189274E-4	1.1353016562726646E-4	0.0	0.21067414401566414	5.96033369543149E-4
82-83	2.2706033125453293E-4	1.1353016562726646E-4	0.0	0.22389094746410507	6.811809937635987E-4
84-85	2.2706033125453293E-4	1.3245185989847755E-4	0.0	0.23747672395083463	6.811809937635987E-4
86-87	2.2706033125453293E-4	1.3245185989847755E-4	0.0	0.25164907295997174	6.906418408992043E-4
88-89	2.2706033125453293E-4	1.3245185989847755E-4	0.0	0.26688103684829667	7.001026880348099E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	11825	0.0	38.58518	1
GGTATCA	7145	0.0	34.06237	1
TCAACGC	14610	0.0	30.885817	4
ATCAACG	15070	0.0	30.005808	3
CAACGCA	15110	0.0	29.912766	5
TATCAAC	15190	0.0	29.799469	2
AACGCAG	16135	0.0	28.014103	6
GTACCTG	5740	0.0	27.71346	1
TACCTGG	6380	0.0	24.716717	2
ACGCAGA	19010	0.0	23.777584	7
GTACATG	19080	0.0	23.36435	1
TACATGG	19245	0.0	22.656778	2
CGCAGAG	19985	0.0	22.57004	8
ACATGGG	19185	0.0	21.787252	3
GAGTACA	14495	0.0	20.27324	1
GCAGAGT	22180	0.0	20.12161	9
AGTACAT	14025	0.0	19.303913	2
CATGGGG	14210	0.0	19.01971	4
AGAGTAC	18340	0.0	18.833193	10-11
ACCTGGG	8305	0.0	18.702072	3
>>END_MODULE