FastQCFastQC Report
Mon 15 Aug 2016
SRR2921454_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921454_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4637656
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG70800.043.6910441
TACCTGG74200.040.90162
GTATCAA73600.040.4027671
ACCTGGG91650.032.363693
TCAACGC91850.031.0949884
GGTATCA44100.030.4789961
TATCAAC95450.030.3547972
CAACGCA94700.030.0139545
ATCAACG96950.029.5068573
AACGCAG100600.028.6120836
GTACATG136150.025.8980771
CCTGGGG88350.025.4774784
TACATGG140550.023.811032
ACGCAGA120500.023.692157
ACATGGG144100.022.1745593
CGCAGAG131950.021.6329928
GAGTACA101900.020.0117911
GCAGAGT148050.018.78519
CATGGGG114250.018.7789424
AGTACAT98100.018.5527232
Produced by FastQC (version 0.11.5)