##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921454_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4637656
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.610968773880597	31.0	30.0	34.0	26.0	34.0
2	29.687919069460953	31.0	30.0	34.0	25.0	34.0
3	29.814028681730598	31.0	31.0	34.0	25.0	34.0
4	33.28891038921386	37.0	35.0	37.0	27.0	37.0
5	33.237180161702376	37.0	35.0	37.0	27.0	37.0
6	33.33445796755947	37.0	35.0	37.0	28.0	37.0
7	33.19340675548165	37.0	35.0	37.0	27.0	37.0
8	33.157219509165834	37.0	35.0	37.0	27.0	37.0
9	34.51348978880711	38.0	35.0	39.0	27.0	39.0
10-11	34.35099444633237	38.0	35.0	39.0	27.0	39.0
12-13	34.1483059545598	38.0	35.0	39.0	25.5	39.0
14-15	35.033422056314656	39.0	35.0	40.5	25.0	41.0
16-17	34.846073318072754	39.0	34.5	40.0	24.5	41.0
18-19	34.62366947871942	39.0	34.0	40.0	22.5	41.0
20-21	34.48185182342114	39.0	34.0	40.0	21.5	41.0
22-23	34.50050833869524	39.0	34.0	40.0	20.5	41.0
24-25	34.32860533424644	39.0	34.0	40.0	18.5	41.0
26-27	34.00373108742865	38.0	34.0	40.0	17.5	41.0
28-29	33.71771019670281	38.0	33.0	40.0	16.0	41.0
30-31	33.41341196932244	38.0	33.0	40.0	12.5	41.0
32-33	33.09268399812319	38.0	32.5	40.0	9.0	41.0
34-35	32.62687519298542	38.0	31.5	40.0	8.5	41.0
36-37	32.19311026949821	38.0	31.0	40.0	7.0	41.0
38-39	31.909791929371217	37.0	30.0	40.0	2.0	41.0
40-41	31.591113053663317	37.0	30.0	40.0	2.0	41.0
42-43	31.255469142170096	37.0	30.0	40.0	2.0	41.0
44-45	30.885594576225575	36.0	29.5	39.0	2.0	40.0
46-47	30.548122262625775	36.0	28.5	39.0	2.0	40.0
48-49	30.277167280194995	36.0	28.0	39.0	2.0	40.0
50-51	29.597574076214364	35.0	27.0	38.5	2.0	39.5
52-53	30.192386196820117	35.5	28.5	38.5	2.0	40.0
54-55	30.5802797792678	36.0	29.0	39.0	2.0	40.0
56-57	30.393884000883205	36.0	29.0	39.0	2.0	40.0
58-59	30.014561989936297	35.5	28.0	39.0	2.0	40.0
60-61	29.51030078556926	35.0	27.5	39.0	2.0	40.0
62-63	28.96956242550116	34.5	26.5	38.0	2.0	40.0
64-65	28.375672430210432	34.0	26.0	38.0	2.0	40.0
66-67	27.843256054351595	34.0	25.0	37.0	2.0	39.5
68-69	27.27826514083839	33.5	23.5	36.5	2.0	39.0
70-71	26.710546879716823	33.0	22.0	36.0	2.0	39.0
72-73	26.10943459368267	32.5	20.0	35.0	2.0	38.0
74-75	25.46431721110837	32.0	18.0	35.0	2.0	37.0
76-77	24.822291692182432	31.5	11.5	35.0	2.0	36.5
78-79	24.206486099874592	31.0	7.0	34.5	2.0	36.0
80-81	23.565544102451756	31.0	2.0	34.0	2.0	35.5
82-83	22.878878791354943	30.0	2.0	34.0	2.0	35.0
84-85	22.19468153739734	29.5	2.0	34.0	2.0	35.0
86-87	21.501515420721155	29.0	2.0	34.0	2.0	35.0
88-89	20.78645009461676	29.0	2.0	34.0	2.0	35.0
90-91	19.985217963557453	27.0	2.0	33.0	2.0	35.0
92-93	19.110150256940145	25.5	2.0	33.0	2.0	34.0
94-95	18.152370076607667	24.5	2.0	32.5	2.0	34.0
96-97	17.234452382841674	21.5	2.0	32.0	2.0	34.0
98	16.619700555625514	18.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	215337.0
3	36049.0
4	27262.0
5	22978.0
6	21660.0
7	22829.0
8	26004.0
9	29801.0
10	33568.0
11	35995.0
12	38697.0
13	40984.0
14	36772.0
15	37191.0
16	41270.0
17	46860.0
18	51630.0
19	51923.0
20	54318.0
21	59064.0
22	63500.0
23	69688.0
24	76548.0
25	84406.0
26	93773.0
27	105366.0
28	118849.0
29	135818.0
30	157067.0
31	185223.0
32	223232.0
33	276342.0
34	353791.0
35	460906.0
36	570269.0
37	527088.0
38	197994.0
39	7604.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.08339721737782	16.509767164590354	12.408487324748501	24.998348293283325
2	16.29344457260902	18.32818361987715	38.92212610718121	26.456245700332627
3	20.869949797312312	22.224257369137536	26.914357709790927	29.991435123759224
4	13.963832093758258	14.006698559432188	33.93194651939184	38.09752282741771
5	15.672770321862108	35.1784180494266	32.349796855275216	16.79901477343608
6	35.75712763384413	32.256382296329974	16.698429904291714	15.288060165534187
7	32.25412396307718	29.37942962557746	18.746775120409886	19.61967129093548
8	28.814357651166116	33.48287602470927	18.257536496590433	19.445229827534185
9	27.426909563928735	16.351917521433506	18.37044966587106	37.85072324876669
10-11	27.350480158550443	24.6030200495578	25.49625728648418	22.55024250540758
12-13	27.23017502099603	21.484967231747095	26.373060475355047	24.911797271901833
14-15	24.131070547998824	23.034477686969527	23.820945400247012	29.013506364784636
16-17	24.87401182973846	26.169126118641355	24.27258322519149	24.6842788264287
18-19	24.892779603363007	25.40690031305044	25.676640823456097	24.02367926013045
20-21	25.243474991084913	24.750520473953642	25.767752356361868	24.238252178599577
22-23	25.04723212261326	24.379372552803012	25.627226508824453	24.946168815759272
24-25	24.753593618565866	24.632525200021274	25.76545674846391	24.848424432948953
26-27	24.82686320056419	24.77746644948497	25.708958831293156	24.686711518657685
28-29	24.822944204063578	24.84107167905737	25.64949232279854	24.686491794080517
30-31	24.957493807719914	24.88690364853177	25.54264566825171	24.612956875496607
32-33	24.83381326239133	24.89558698571731	25.468029240281343	24.80257051161002
34-35	24.908850261600588	24.87249535065172	25.64138169442025	24.57727269332744
36-37	25.021589065893373	24.836380317714248	25.46999946956145	24.67203114683092
38-39	24.979134895583407	24.889624736028512	25.51582723064717	24.615413137740912
40-41	25.012226235055724	24.82021661495203	25.57915927518347	24.588397874808773
42-43	24.95089688378109	24.717005426325166	25.536806906886273	24.79529078300747
44-45	24.8767060405425	24.877854725201036	25.511560210690437	24.733879023566026
46-47	25.031883505202675	24.835132209545876	25.420434265801706	24.71255001944975
48-49	25.036455029407257	24.928566127456904	25.26091118342468	24.774067659711164
50-51	24.782538780232024	25.163216464746004	25.28434069000282	24.769904065019155
52-53	24.919570855134925	25.09291714895234	25.259322650224508	24.728189345688236
54-55	24.80555517007499	24.963105895944533	25.434153510696795	24.797185423283683
56-57	24.80267905454135	25.07308132994312	25.413446617928802	24.71079299758673
58-59	24.90290138716019	25.02126808491459	25.36757058013875	24.708259947786473
60-61	24.920580861453168	24.90469540835638	25.40891225931128	24.76581147087918
62-63	24.74915808039621	25.097911931010003	25.39764885653365	24.755281132060137
64-65	24.814505093095665	24.937942546520734	25.406512767289293	24.84103959309431
66-67	24.815804307547257	25.04961668972625	25.358196991567034	24.77638201115946
68-69	24.777845280744305	25.00797067226091	25.39032452502704	24.823859521967737
70-71	24.737836542752444	24.84254378705967	25.448593692999705	24.971025977188184
72-73	24.84317026780964	24.93110559505847	25.30881598222407	24.916908154907823
74-75	24.68713423381967	25.09680318328235	25.287626884660153	24.928435698237823
76-77	24.858901387070375	24.9100898706037	25.257526152745264	24.97348258958066
78-79	24.795619968463463	24.84000544883477	25.419734614026073	24.9446399686757
80-81	24.74635535555403	24.97890964231338	25.31005343323736	24.964681568895223
82-83	24.917198299324223	24.905826670822545	25.27515550431223	24.901819525541
84-85	24.874612274063367	24.93896530631026	25.186027250434417	25.000395169191957
86-87	24.823865109684725	24.90703743783323	25.362752936934868	24.906344515547175
88-89	24.824466577011073	24.69243070120017	25.35491680938425	25.128185912404504
90-91	24.82661572939201	24.749586131034125	25.335342411940303	25.088455727633562
92-93	24.82933278185671	24.896625201254682	25.331010490687877	24.943031526200734
94-95	25.015728477000472	24.742069220025638	25.26964727712721	24.97255502584668
96-97	24.913525607410534	24.69607952796378	25.276585414394386	25.1138094502313
98	24.91646682948146	24.75892857529549	25.163742338010614	25.160862257212436
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	454.0
1	326.0
2	333.5
3	487.5
4	417.0
5	369.5
6	570.0
7	1064.0
8	1578.0
9	1687.0
10	1505.5
11	1483.0
12	1726.0
13	2062.0
14	2433.0
15	2812.0
16	3118.0
17	3359.0
18	3500.5
19	3492.0
20	3442.5
21	3273.5
22	3242.0
23	3502.0
24	3902.0
25	4536.5
26	5636.5
27	7160.0
28	9204.0
29	11915.5
30	15187.0
31	19430.0
32	25140.0
33	31361.0
34	38746.0
35	47910.0
36	57882.5
37	68753.5
38	80505.0
39	92859.0
40	105525.0
41	119004.5
42	133381.5
43	147800.0
44	161530.5
45	175260.0
46	189686.0
47	203416.5
48	212557.5
49	216293.0
50	217281.0
51	217182.5
52	218463.5
53	220073.5
54	217982.5
55	210606.0
56	198207.0
57	184683.0
58	169659.0
59	151630.0
60	132597.0
61	112222.0
62	91302.0
63	70633.0
64	52626.0
65	39732.0
66	30351.0
67	22940.0
68	17226.0
69	13077.5
70	9780.5
71	7283.0
72	5591.0
73	4092.5
74	2983.5
75	2191.5
76	1504.5
77	912.5
78	553.5
79	332.5
80	201.5
81	129.5
82	78.0
83	46.5
84	29.0
85	21.0
86	25.5
87	19.0
88	11.0
89	11.5
90	9.5
91	9.0
92	9.5
93	8.5
94	7.0
95	7.5
96	8.0
97	7.0
98	5.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4575371696391453
2	0.04577743584258945
3	0.0527421611262241
4	0.05026246017384644
5	0.060008763047539534
6	0.06130252006617136
7	0.03933021336640751
8	0.01839291228154913
9	0.011298811296051281
10-11	0.013929450567269326
12-13	0.010123648670794039
14-15	0.014166639354018497
16-17	0.013228665515510423
18-19	0.05305481907239346
20-21	0.07888683421107559
22-23	0.09533911096467698
24-25	0.06655301730011885
26-27	0.08081668843053474
28-29	0.08206732021521217
30-31	0.0528068489771557
32-33	0.029767192737020595
34-35	0.38645600277381503
36-37	0.6094134623180331
38-39	0.5582345909226558
40-41	0.5530272189226627
42-43	0.5262356673284952
44-45	0.5106674578709589
46-47	0.50027427648795
48-49	0.48997812688133835
50-51	0.5042417980117542
52-53	0.5871824042145429
54-55	0.5564880189475028
56-57	0.5129638765790304
58-59	0.49076516240100604
60-61	0.503950702682562
62-63	0.5162845195935188
64-65	0.5346127440241364
66-67	0.5617600787984275
68-69	0.5146349793947632
70-71	0.4625612593948322
72-73	0.444761319080156
74-75	0.42653012642593585
76-77	0.4400175433451727
78-79	0.4345945451754075
80-81	0.4317806236598834
82-83	0.45072338267435097
84-85	0.41790507963505696
86-87	0.42119337872407964
88-89	0.4469714873203187
90-91	0.43018499000357074
92-93	0.42588324791661997
94-95	0.43631955453358334
96-97	0.4303790535563655
98	0.42543043296009886
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4637656.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.012241396391225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.07035162625218	42.04858657001607
2	13.676974329223121	15.321559754013828
3	4.775515057437688	8.024619065679026
4	2.1136801851721043	4.7356785906651515
5	1.1750325792742764	3.2908104239467493
6	0.6791448998063507	2.282425684264875
7	0.47638549035181166	1.8678393358326713
8	0.3407879887414209	1.52706392723001
9	0.25693486473206023	1.2952347899869157
>10	1.3229529003834557	13.343170393207837
>50	0.08173117436868836	3.121254558748595
>100	0.030004973383933525	2.7675413889501526
>500	3.10111354742645E-4	0.11989663849976985
>1k	1.9381951824446914E-4	0.25431887895826333
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.1562616977197103E-5	0.0
2	0.0	2.1562616977197103E-5	0.0	4.3125233954394206E-5	0.0
3	0.0	2.1562616977197103E-5	0.0	4.3125233954394206E-5	0.0
4	0.0	2.1562616977197103E-5	0.0	4.3125233954394206E-5	0.0
5	0.0	2.1562616977197103E-5	0.0	4.3125233954394206E-5	0.0
6	4.3125233954394206E-5	2.1562616977197103E-5	0.0	4.3125233954394206E-5	2.1562616977197103E-5
7	4.3125233954394206E-5	2.1562616977197103E-5	0.0	4.3125233954394206E-5	2.1562616977197103E-5
8	4.3125233954394206E-5	2.1562616977197103E-5	0.0	4.3125233954394206E-5	4.3125233954394206E-5
9	4.3125233954394206E-5	2.1562616977197103E-5	0.0	6.46878509315913E-5	4.3125233954394206E-5
10-11	4.3125233954394206E-5	2.1562616977197103E-5	0.0	6.46878509315913E-5	4.3125233954394206E-5
12-13	4.3125233954394206E-5	2.1562616977197103E-5	0.0	6.46878509315913E-5	1.6171962732897826E-4
14-15	4.3125233954394206E-5	2.1562616977197103E-5	0.0	6.46878509315913E-5	2.0484486128337245E-4
16-17	4.3125233954394206E-5	2.1562616977197103E-5	0.0	8.625046790878841E-5	2.587514037263652E-4
18-19	4.3125233954394206E-5	2.1562616977197103E-5	0.0	1.0781308488598551E-4	2.8031402070356233E-4
20-21	4.3125233954394206E-5	2.1562616977197103E-5	0.0	1.1859439337458406E-4	3.557831801237522E-4
22-23	4.3125233954394206E-5	4.3125233954394206E-5	0.0	1.293757018631826E-4	3.9890841407814635E-4
24-25	4.3125233954394206E-5	4.3125233954394206E-5	0.0	1.9406355279477392E-4	4.096897225667449E-4
26-27	4.3125233954394206E-5	4.3125233954394206E-5	0.0	2.6953271221496377E-4	4.096897225667449E-4
28-29	4.3125233954394206E-5	4.3125233954394206E-5	0.0	5.390654244299275E-4	4.096897225667449E-4
30-31	4.3125233954394206E-5	6.46878509315913E-5	0.0	0.0014339140289836073	4.3125233954394203E-4
32-33	4.3125233954394206E-5	6.46878509315913E-5	0.0	0.003342205631465551	4.528149565211391E-4
34-35	4.3125233954394206E-5	1.1859439337458406E-4	0.0	0.005325966393367684	4.743775734983362E-4
36-37	4.3125233954394206E-5	1.4015701035178114E-4	0.0	0.008581921556924447	4.743775734983362E-4
38-39	4.3125233954394206E-5	1.8328224430617536E-4	0.0	0.013713824397497357	4.743775734983362E-4
40-41	4.3125233954394206E-5	2.2640747826056958E-4	0.0	0.020020889863327507	4.743775734983362E-4
42-43	4.3125233954394206E-5	2.371887867491681E-4	0.0	0.025368418873672388	5.175028074527304E-4
44-45	4.3125233954394206E-5	2.371887867491681E-4	0.0	0.03139517031879898	5.821906583843217E-4
46-47	4.3125233954394206E-5	2.371887867491681E-4	0.0	0.03857552177220562	6.46878509315913E-4
48-49	4.3125233954394206E-5	2.371887867491681E-4	0.0	0.045831342385032434	7.331289772247014E-4
50-51	4.3125233954394206E-5	2.4797009523776665E-4	0.0	0.05315185084879086	7.439102857133E-4
52-53	5.3906542442992754E-5	2.587514037263652E-4	0.0	0.06111923782186519	9.164112215308768E-4
54-55	6.46878509315913E-5	2.587514037263652E-4	0.0	0.06875240423179296	9.703177639738696E-4
56-57	6.46878509315913E-5	2.587514037263652E-4	0.0	0.07701088653405945	9.703177639738696E-4
58-59	6.46878509315913E-5	2.587514037263652E-4	0.0	0.0854526510806321	9.703177639738696E-4
60-61	6.46878509315913E-5	2.587514037263652E-4	0.0	0.09442269974314611	0.0010350056149054608
62-63	1.509383188403797E-4	2.587514037263652E-4	0.0	0.10330649793775132	0.0010996934658370522
64-65	1.509383188403797E-4	2.587514037263652E-4	0.0	0.11200701388805034	0.0011428186997914465
66-67	1.509383188403797E-4	2.587514037263652E-4	0.0	0.12163472236836885	0.0011643813167686436
68-69	1.509383188403797E-4	2.587514037263652E-4	0.0	0.1308203972006548	0.0011643813167686436
70-71	1.509383188403797E-4	2.587514037263652E-4	0.0	0.14086857671202868	0.0011859439337458405
72-73	1.509383188403797E-4	2.8031402070356233E-4	0.0	0.15060409827723314	0.0013800074865406146
74-75	1.509383188403797E-4	2.8031402070356233E-4	0.0	0.16054446470372102	0.0014986018799151984
76-77	1.509383188403797E-4	2.8031402070356233E-4	2.1562616977197103E-5	0.17046326851323168	0.0015093831884037971
78-79	1.509383188403797E-4	2.8031402070356233E-4	2.1562616977197103E-5	0.18180520504323736	0.0015093831884037971
80-81	1.509383188403797E-4	2.8031402070356233E-4	2.1562616977197103E-5	0.19381558269953614	0.0015632897308467898
82-83	1.509383188403797E-4	2.8031402070356233E-4	2.1562616977197103E-5	0.20510361268709884	0.0017250093581757681
84-85	1.509383188403797E-4	2.8031402070356233E-4	2.1562616977197103E-5	0.21693070809909143	0.0017465719751529653
86	1.509383188403797E-4	2.8031402070356233E-4	2.1562616977197103E-5	0.22636435302661517	0.0017681345921301624
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	7080	0.0	43.691044	1
TACCTGG	7420	0.0	40.9016	2
GTATCAA	7360	0.0	40.402767	1
ACCTGGG	9165	0.0	32.36369	3
TCAACGC	9185	0.0	31.094988	4
GGTATCA	4410	0.0	30.478996	1
TATCAAC	9545	0.0	30.354797	2
CAACGCA	9470	0.0	30.013954	5
ATCAACG	9695	0.0	29.506857	3
AACGCAG	10060	0.0	28.612083	6
GTACATG	13615	0.0	25.898077	1
CCTGGGG	8835	0.0	25.477478	4
TACATGG	14055	0.0	23.81103	2
ACGCAGA	12050	0.0	23.69215	7
ACATGGG	14410	0.0	22.174559	3
CGCAGAG	13195	0.0	21.632992	8
GAGTACA	10190	0.0	20.011791	1
GCAGAGT	14805	0.0	18.7851	9
CATGGGG	11425	0.0	18.778942	4
AGTACAT	9810	0.0	18.552723	2
>>END_MODULE