FastQCFastQC Report
Mon 15 Aug 2016
SRR2921454_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921454_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4637656
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG72400.044.2844431
GTATCAA71850.043.8940731
TACCTGG76850.040.9759672
GGTATCA38800.038.554281
TCAACGC89800.034.3807534
TATCAAC94000.032.944232
CAACGCA93950.032.8464625
ACCTGGG93800.032.4078943
ATCAACG95200.032.380343
AACGCAG102950.030.2058946
GTACATG137950.026.591551
TACATGG140850.025.1222782
ACGCAGA124400.024.99817
CCTGGGG91950.023.8137384
ACATGGG143650.023.5422523
CGCAGAG133800.023.028988
GAGTACA103500.020.3909781
CATGGGG112150.019.9480294
AGTACAT98350.019.2206612
GCAGAGT158650.019.0619249
Produced by FastQC (version 0.11.5)