FastQCFastQC Report
Mon 15 Aug 2016
SRR2921453_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921453_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5390473
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68080.1262968945396814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA124900.033.1702351
TCAACGC151300.026.9668164
TATCAAC152900.026.7745762
GGTATCA83050.026.494561
CAACGCA155900.026.0829145
ATCAACG159300.025.612553
GTACATG181550.025.35551
AACGCAG164900.024.6809626
TACATGG188350.023.8771252
ACATGGG195100.022.4400963
ACGCAGA190800.021.2774877
CGCAGAG201150.020.1563388
CATGGGG155050.019.1889654
GAGTACA143950.019.1550641
GTACCTG49550.018.8590931
AGTACAT134500.018.4056032
AGAGTAC163650.018.19683510-11
GCAGAGT222850.017.9063079
GTATATA8900.017.0683821
TCGCGTA3854.2018655E-1016.6597259
Produced by FastQC (version 0.11.5)