##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921453_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5390473
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.368844811948783	31.0	31.0	34.0	27.0	34.0
2	30.46786914617697	31.0	31.0	34.0	27.0	34.0
3	30.60145334184959	31.0	31.0	34.0	27.0	34.0
4	34.12080498316196	37.0	35.0	37.0	30.0	37.0
5	34.10086925581484	37.0	35.0	37.0	30.0	37.0
6	34.202919669572594	37.0	35.0	37.0	30.0	37.0
7	34.08441114536702	37.0	35.0	37.0	30.0	37.0
8	34.06305346488147	37.0	35.0	37.0	30.0	37.0
9	35.51068765208545	39.0	35.0	39.0	30.0	39.0
10-11	35.383696477099505	39.0	35.0	39.0	30.0	39.0
12-13	35.212914525311604	39.0	35.0	39.0	29.5	39.0
14-15	36.215324146879134	40.0	36.0	41.0	29.0	41.0
16-17	36.05867221670529	39.5	36.0	41.0	28.5	41.0
18-19	35.86725506277464	39.0	36.0	41.0	27.0	41.0
20-21	35.74724778326503	39.0	36.0	40.0	27.0	41.0
22-23	35.78613425946109	39.0	36.0	41.0	27.0	41.0
24-25	35.64502113265385	39.0	36.0	41.0	27.0	41.0
26-27	35.367818927949365	39.0	35.5	40.0	26.5	41.0
28-29	35.12564194273861	39.0	35.0	40.0	25.0	41.0
30-31	34.86563229237954	38.5	34.5	40.0	24.5	41.0
32-33	34.586722723590306	38.0	34.0	40.0	24.0	41.0
34-35	34.15572260541886	38.0	33.5	40.0	21.0	41.0
36-37	33.761385318134415	38.0	33.0	40.0	18.0	41.0
38-39	33.51653788081306	38.0	33.0	40.0	16.5	41.0
40-41	33.235715400114245	38.0	32.5	40.0	15.0	41.0
42-43	32.937470700623116	38.0	32.0	40.0	14.0	41.0
44-45	32.60846923822826	37.0	31.5	40.0	9.0	41.0
46-47	32.304345926600504	37.0	31.0	40.0	8.5	41.0
48-49	32.07655571227237	37.0	31.0	40.0	8.0	41.0
50-51	31.335660432767217	36.0	30.5	38.5	7.0	40.0
52-53	31.87725566940044	36.5	31.0	39.0	7.0	40.0
54-55	32.30076599956998	37.0	32.0	39.5	4.0	40.5
56-57	32.13123644251627	37.0	31.5	40.0	2.0	41.0
58-59	31.77252330175849	36.0	31.0	39.0	2.0	41.0
60-61	31.297320940110453	35.5	30.5	39.0	2.0	40.0
62-63	30.785570858067558	35.0	30.0	39.0	2.0	40.0
64-65	30.22243864313948	35.0	29.0	38.0	2.0	40.0
66-67	29.707286355019306	34.5	28.5	37.5	2.0	40.0
68-69	29.16889835084973	34.0	28.0	37.0	2.0	39.0
70-71	28.62687838339975	34.0	27.0	36.0	2.0	39.0
72-73	28.056573514049695	34.0	26.0	36.0	2.0	38.5
74-75	27.448732884850735	33.0	26.0	35.0	2.0	37.5
76-77	26.84929161132984	33.0	25.0	35.0	2.0	37.0
78-79	26.26659794047758	32.0	23.5	35.0	2.0	36.0
80-81	25.667823862581262	32.0	21.0	34.5	2.0	36.0
82-83	25.02077313066961	31.0	19.0	34.0	2.0	35.0
84-85	24.380107181688878	31.0	16.5	34.0	2.0	35.0
86-87	23.729948559245173	31.0	7.5	34.0	2.0	35.0
88-89	23.045127857982035	30.0	2.0	34.0	2.0	35.0
90-91	22.266298337826754	29.5	2.0	34.0	2.0	35.0
92-93	21.409436889861055	29.0	2.0	34.0	2.0	35.0
94-95	20.451221256464876	28.0	2.0	33.5	2.0	34.5
96-97	19.526672241934985	27.0	2.0	33.0	2.0	34.0
98	18.88648881090769	25.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	161448.0
3	29334.0
4	22787.0
5	19450.0
6	18136.0
7	19259.0
8	21900.0
9	25859.0
10	28562.0
11	31951.0
12	35191.0
13	38434.0
14	32709.0
15	33059.0
16	37098.0
17	42131.0
18	47138.0
19	47797.0
20	50152.0
21	54855.0
22	60244.0
23	66458.0
24	74271.0
25	84002.0
26	95376.0
27	108104.0
28	124978.0
29	145427.0
30	172845.0
31	207878.0
32	257573.0
33	329800.0
34	439675.0
35	600954.0
36	780470.0
37	753467.0
38	281923.0
39	9778.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.1635318868866	16.689223710264297	13.114368777746236	25.032875625102868
2	16.318955686616896	18.323295856125537	38.46078033961121	26.89696811764636
3	20.764035024706736	21.413198247688076	27.034076011105178	30.78869071650001
4	14.184337661585566	14.114565661410348	33.675449388107545	38.02564728889655
5	16.142264843386712	35.295187762379584	32.21428644355527	16.34826095067844
6	36.38909700600937	32.17470675287047	15.92519218760094	15.511004053519226
7	32.25114720934656	29.731935802675352	18.420493404416234	19.596423583561847
8	28.548958266650708	33.311605235154246	18.259989405000805	19.879447093194244
9	27.243842832482596	16.311169064027418	18.374616127897504	38.07037197559249
10-11	27.32513755316098	24.505173672433063	25.289863791547624	22.87982498285833
12-13	27.287951160096142	21.47014159007988	26.11654673455965	25.125360515264326
14-15	24.3406595037728	23.051052874150127	23.59575651603413	29.01253110604294
16-17	24.931333839301843	26.259413041277995	24.093600648723246	24.71565247069692
18-19	24.947303435689776	25.486640427898326	25.557286121369525	24.008770015042373
20-21	25.429677364252495	24.692935081549	25.51202242533591	24.365365128862592
22-23	25.224079323532717	24.282943379198095	25.47116221157337	25.02181508569582
24-25	24.965289374028043	24.523625781302357	25.54408755375993	24.966997290909667
26-27	24.938068785888262	24.706246818029733	25.549916874218088	24.80576752186392
28-29	24.936236858266327	24.891823380298298	25.410283892547973	24.761655868887402
30-31	25.07564026935732	24.790481094387548	25.263877808388624	24.870000827866505
32-33	24.93284937624243	24.85148364185938	25.212382289234075	25.003284692664113
34-35	25.05758956742263	24.776616922094654	25.387490994645585	24.77830251583713
36-37	25.188820927438886	24.715030162625226	25.270315552489876	24.825833357446008
38-39	25.089082096399444	24.808777274468014	25.2683891148885	24.83375151424404
40-41	25.186290121711973	24.7642209528927	25.27532354376155	24.77416538163378
42-43	25.145024657059505	24.636858967513785	25.197232694243414	25.020883681183292
44-45	25.053108945745233	24.774215879588766	25.212086105961273	24.960589068704728
46-47	25.175878183478336	24.73032851013489	25.224119802569263	24.869673503817513
48-49	25.17849243429624	24.850440946210576	25.050312943853765	24.920753675639418
50-51	24.946332247468316	25.035331717201174	25.09261026540117	24.92572576992933
52-53	25.095686669376022	24.97260862647461	25.029794330235305	24.901910373914063
54-55	25.04079818442132	24.802385470519468	25.185715350556524	24.97110099450268
56-57	25.02950658059458	24.90324629735192	25.16235962751911	24.904887494534396
58-59	25.11495183024197	24.8246448519853	25.128367538572725	24.9320357792
60-61	25.129260766042773	24.745720958612687	25.202884296928847	24.9221339784157
62-63	24.93047069548701	24.929622094801566	25.14813210866266	24.99177510104876
64-65	25.01382498966798	24.823833396119788	25.172832190787748	24.98950942342448
66-67	25.065231791168706	24.833571235317716	25.06918749475216	25.03200947876142
68-69	24.92269898100108	24.882031902907794	25.148088404875125	25.047180711216
70-71	24.958223854153648	24.698968954747734	25.228458127714724	25.11434906338389
72-73	25.072702557707988	24.731981101947778	25.105700251410557	25.089616088933674
74-75	24.871001541590044	25.022635640078512	25.06287574277416	25.04348707555729
76-77	25.063255021940428	24.73857712565341	25.047097071357477	25.15107078104868
78-79	24.991464908661477	24.74290366789028	25.154265253494074	25.11136616995417
80-81	24.968555738686003	24.86590474916196	25.109790530299463	25.055748981852567
82-83	25.09907332749573	24.751510563037698	25.04520785548695	25.104208253979614
84-85	25.08333588752941	24.79786837508691	24.987509282445426	25.13128645493825
86-87	25.003668356703834	24.747707894275678	25.201186656330364	25.047437092690128
88-89	24.95409041239325	24.642149371989643	25.071732274556574	25.33202794106053
90-91	25.00497082818004	24.611963269353293	25.092367957193666	25.290697945273
92-93	25.06496924715985	24.75944864301317	25.110482425717322	25.06509968410966
94-95	25.1801451037794	24.604998321382052	25.048483313009463	25.166373261829083
96-97	25.08299272671673	24.57396259441008	25.097928575707932	25.245116103165255
98	25.071409977402602	24.649462680565364	24.971774215338094	25.307353126693943
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	783.0
1	577.0
2	549.0
3	778.5
4	734.5
5	704.5
6	1222.0
7	2632.0
8	3833.0
9	3663.5
10	2831.5
11	2363.5
12	2477.0
13	2823.5
14	3237.5
15	3696.5
16	4028.0
17	4173.5
18	4182.5
19	4023.5
20	3718.5
21	3359.0
22	3045.0
23	2904.0
24	2963.5
25	3179.0
26	3616.0
27	4355.0
28	5503.0
29	7240.5
30	9906.0
31	13947.0
32	19714.0
33	26932.0
34	35720.0
35	46712.0
36	59446.5
37	73516.0
38	88448.5
39	103752.0
40	119123.0
41	135821.0
42	154681.0
43	172150.5
44	188029.5
45	207128.0
46	228023.0
47	246411.5
48	256570.0
49	258339.0
50	258377.0
51	258903.0
52	261235.0
53	263806.5
54	260134.5
55	249472.5
56	233756.0
57	217015.0
58	199908.0
59	180130.5
60	158273.5
61	133013.0
62	107532.0
63	83788.5
64	62496.5
65	46669.5
66	35594.5
67	27133.0
68	20740.0
69	15660.0
70	11732.5
71	8856.0
72	6572.0
73	4678.0
74	3428.5
75	2480.0
76	1635.5
77	993.0
78	591.0
79	355.5
80	214.0
81	126.0
82	91.5
83	67.5
84	39.5
85	30.5
86	21.5
87	16.0
88	19.5
89	15.5
90	13.0
91	11.5
92	10.0
93	9.5
94	5.0
95	6.0
96	8.0
97	5.5
98	2.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47409568696476173
2	0.04943907519803921
3	0.05706363801469742
4	0.05426239960760401
5	0.06452123960179376
6	0.06583837819055953
7	0.04448589205437074
8	0.021111319915710552
9	0.01218816975801567
10-11	0.015388259991284625
12-13	0.01146467109658095
14-15	0.0156758043310856
16-17	0.015017235036702716
18-19	0.05611752438051355
20-21	0.08534501517770333
22-23	0.10088168515082072
24-25	0.0703834338841879
26-27	0.08643026316985541
28-29	0.08757116490519477
30-31	0.05808395663979766
32-33	0.03415284706926461
34-35	0.3978315075504506
36-37	0.6247689210204745
38-39	0.5741703928393669
40-41	0.5693006903104793
42-43	0.5422436954975937
44-45	0.5265215130471853
46-47	0.5172737160542312
48-49	0.5062728261508777
50-51	0.5209468631045922
52-53	0.6027393143421736
54-55	0.5724822292960191
56-57	0.5293320270781433
58-59	0.5075064841248625
60-61	0.5204923575352293
62-63	0.532513566063683
64-65	0.5511482944075594
66-67	0.5773704830726357
68-69	0.5313448374567501
70-71	0.47989295188010406
72-73	0.46278870147387813
74-75	0.44404266564362715
76-77	0.45809523579841693
78-79	0.4524834833603656
80-81	0.44952455934757485
82-83	0.46851176139830397
84-85	0.4357224310371279
86-87	0.43833815696693035
88-89	0.4644490381456321
90-91	0.4482723501258609
92-93	0.4432913401105988
94-95	0.45360583384797587
96-97	0.4487454069429529
98	0.4438571531663362
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	5390473.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.715087004135064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.86091085751636	35.228563484736846
2	14.801359999588875	14.717018003181709
3	5.673336680326961	8.461512799986169
4	2.742773179385587	5.4542882898305045
5	1.5407760701117068	3.8299908189746406
6	0.964680753374212	2.877551255112811
7	0.6362598038520825	2.2142198054018136
8	0.47748951535424144	1.899074623951872
9	0.3562679070492306	1.5940700996160244
>10	1.7934444928768971	16.019820012733295
>50	0.11038735850064123	3.7340577245817075
>100	0.0410387257312972	3.2759382477779706
>500	0.0010122291630639613	0.31819033157288124
>1k	2.249375733124499E-4	0.24881710035698332
>5k	3.7489595549175465E-5	0.1268874021848005
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6808	0.1262968945396814	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.275623864547693E-5
2	0.0	0.0	0.0	0.0	9.275623864547693E-5
3	0.0	0.0	0.0	0.0	9.275623864547693E-5
4	0.0	0.0	0.0	3.7102495458190776E-5	9.275623864547693E-5
5	0.0	0.0	0.0	3.7102495458190776E-5	9.275623864547693E-5
6	0.0	0.0	0.0	3.7102495458190776E-5	9.275623864547693E-5
7	0.0	0.0	0.0	3.7102495458190776E-5	9.275623864547693E-5
8	0.0	0.0	0.0	3.7102495458190776E-5	9.275623864547693E-5
9	0.0	0.0	0.0	9.275623864547693E-5	1.1130748637457233E-4
10-11	0.0	0.0	0.0	1.0203186251002462E-4	1.1130748637457233E-4
12-13	0.0	0.0	0.0	1.6696122956185848E-4	1.8551247729095387E-4
14-15	0.0	0.0	0.0	1.6696122956185848E-4	2.0406372502004925E-4
16-17	0.0	0.0	0.0	1.7623685342640618E-4	2.4116622047824006E-4
18-19	0.0	0.0	0.0	1.8551247729095387E-4	2.782687159364308E-4
20-21	0.0	0.0	0.0	1.8551247729095387E-4	2.875443398009785E-4
22-23	0.0	0.0	0.0	2.2261497274914466E-4	3.3392245912371697E-4
24-25	0.0	0.0	0.0	2.4116622047824006E-4	3.7102495458190773E-4
26-27	0.0	0.0	0.0	3.895762023110031E-4	3.803005784464554E-4
28-29	0.0	0.0	0.0	8.904598909965786E-4	3.895762023110031E-4
30-31	0.0	0.0	0.0	0.0032371927287271453	3.988518261755508E-4
32-33	0.0	0.0	0.0	0.007772972798490967	4.2667869776919393E-4
34-35	0.0	0.0	0.0	0.013774301438853324	4.637811932273847E-4
36-37	0.0	0.0	0.0	0.02360646273527388	4.637811932273847E-4
38-39	0.0	0.0	0.0	0.03859587090038295	5.008836886855755E-4
40-41	0.0	0.0	0.0	0.05459632206672772	5.379861841437663E-4
42-43	0.0	0.0	0.0	0.07008661392052237	5.565374318728617E-4
44-45	0.0	0.0	0.0	0.08778450425407938	5.843643034665047E-4
46-47	0.0	0.0	0.0	0.10581631704676009	6.121911750601478E-4
48-49	0.0	0.0	0.0	0.12371827110533715	6.121911750601478E-4
50-51	0.0	0.0	9.275623864547694E-6	0.14235299944921345	6.121911750601478E-4
52-53	0.0	0.0	1.8551247729095388E-5	0.1601343703975514	6.307424227892432E-4
54-55	0.0	3.7102495458190776E-5	1.8551247729095388E-5	0.17824038818114848	6.678449182474339E-4
56-57	0.0	3.7102495458190776E-5	1.8551247729095388E-5	0.19857255569223703	6.678449182474339E-4
58-59	0.0	3.7102495458190776E-5	2.7826871593643082E-5	0.21977663184659307	6.771205421119816E-4
60-61	0.0	5.5653743187286164E-5	3.7102495458190776E-5	0.2405169268077217	6.863961659765293E-4
62-63	0.0	5.5653743187286164E-5	3.7102495458190776E-5	0.2613407023836313	7.234986614347201E-4
64-65	0.0	5.5653743187286164E-5	3.7102495458190776E-5	0.28181200425268804	7.234986614347201E-4
66-67	1.8551247729095388E-5	5.5653743187286164E-5	3.7102495458190776E-5	0.30392509154576974	7.234986614347201E-4
68-69	1.8551247729095388E-5	5.5653743187286164E-5	3.7102495458190776E-5	0.3270677730878162	7.234986614347201E-4
70-71	1.8551247729095388E-5	5.5653743187286164E-5	3.7102495458190776E-5	0.34964464157412534	7.327742852992678E-4
72-73	1.8551247729095388E-5	5.5653743187286164E-5	3.7102495458190776E-5	0.3734087899150965	7.698767807574585E-4
74-75	1.8551247729095388E-5	5.5653743187286164E-5	3.7102495458190776E-5	0.3968946695401313	7.884280284865539E-4
76-77	1.8551247729095388E-5	6.492936705183386E-5	3.7102495458190776E-5	0.4207608497436125	8.069792762156493E-4
78-79	1.8551247729095388E-5	7.420499091638155E-5	3.7102495458190776E-5	0.44751174896896806	8.162549000801971E-4
80-81	1.8551247729095388E-5	7.420499091638155E-5	3.7102495458190776E-5	0.4741884432034072	8.255305239447448E-4
82-83	1.8551247729095388E-5	7.420499091638155E-5	3.7102495458190776E-5	0.5009207911810336	8.811842671320309E-4
84-85	1.8551247729095388E-5	9.275623864547693E-5	3.7102495458190776E-5	0.5283024328291784	8.904598909965786E-4
86	1.8551247729095388E-5	9.275623864547693E-5	3.7102495458190776E-5	0.5486160490925379	8.904598909965786E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	12490	0.0	33.170235	1
TCAACGC	15130	0.0	26.966816	4
TATCAAC	15290	0.0	26.774576	2
GGTATCA	8305	0.0	26.49456	1
CAACGCA	15590	0.0	26.082914	5
ATCAACG	15930	0.0	25.61255	3
GTACATG	18155	0.0	25.3555	1
AACGCAG	16490	0.0	24.680962	6
TACATGG	18835	0.0	23.877125	2
ACATGGG	19510	0.0	22.440096	3
ACGCAGA	19080	0.0	21.277487	7
CGCAGAG	20115	0.0	20.156338	8
CATGGGG	15505	0.0	19.188965	4
GAGTACA	14395	0.0	19.155064	1
GTACCTG	4955	0.0	18.859093	1
AGTACAT	13450	0.0	18.405603	2
AGAGTAC	16365	0.0	18.196835	10-11
GCAGAGT	22285	0.0	17.906307	9
GTATATA	890	0.0	17.068382	1
TCGCGTA	385	4.2018655E-10	16.659725	9
>>END_MODULE