FastQCFastQC Report
Mon 15 Aug 2016
SRR2921453_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921453_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5390473
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56490.10479599842165985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109900.034.2780651
TCAACGC135700.027.406394
CAACGCA138450.026.814265
TATCAAC140400.026.6570952
ATCAACG142650.026.0708873
GTACATG184350.025.8083291
AACGCAG147750.025.3513776
TACATGG189100.024.7148112
GGTATCA73550.022.9222951
ACATGGG198800.022.7211323
GAGTACA141800.021.495191
ACGCAGA174600.021.2902957
CATGGGG154000.020.5410844
AGTACAT137150.020.4319552
CGCAGAG186050.019.8779668
GTACCTG51900.019.5456331
AGAGTAC150800.018.1328110-11
GTGTAGC30750.017.1915651
GCAGAGT210400.017.057849
TACCTGG56350.016.267362
Produced by FastQC (version 0.11.5)