##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921453_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5390473
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.992581727058088	33.0	31.0	34.0	30.0	34.0
2	32.10792244020144	34.0	31.0	34.0	30.0	34.0
3	32.234294467294426	34.0	31.0	34.0	30.0	34.0
4	35.772405872360366	37.0	35.0	37.0	35.0	37.0
5	35.586363756946746	37.0	35.0	37.0	33.0	37.0
6	35.62429697727825	37.0	35.0	37.0	35.0	37.0
7	35.555661441955095	37.0	35.0	37.0	33.0	37.0
8	35.50576025517612	37.0	35.0	37.0	33.0	37.0
9	37.13729592931826	39.0	37.0	39.0	34.0	39.0
10-11	37.05332537608481	39.0	37.0	39.0	33.5	39.0
12-13	36.93754128812073	39.0	37.0	39.0	33.0	39.0
14-15	38.24664663008237	40.0	38.0	41.0	33.0	41.0
16-17	38.151632426319544	40.0	38.0	41.0	33.0	41.0
18-19	38.040043888541874	40.0	38.0	41.0	33.0	41.0
20-21	37.92457340942066	40.0	38.0	41.0	33.0	41.0
22-23	37.89511180187713	40.0	38.0	41.0	33.0	41.0
24-25	37.95186702539833	40.0	38.0	41.0	33.0	41.0
26-27	37.85194638763611	40.0	38.0	41.0	33.0	41.0
28-29	37.720067700923465	40.0	38.0	41.0	33.0	41.0
30-31	37.58871791028356	40.0	38.0	41.0	32.0	41.0
32-33	37.436227674269034	40.0	37.0	41.0	32.0	41.0
34-35	37.299300822024335	40.0	37.0	41.0	32.0	41.0
36-37	37.21082231559271	40.0	37.0	41.0	31.5	41.0
38-39	37.187182460611524	40.0	37.0	41.0	31.0	41.0
40-41	37.02852968561386	40.0	37.0	41.0	31.0	41.0
42-43	36.801309272859726	40.0	36.0	41.0	31.0	41.0
44-45	36.60485220870228	39.0	36.0	41.0	30.0	41.0
46-47	36.349287066274144	39.0	35.5	40.5	30.0	41.0
48-49	36.029989297785185	39.0	35.0	40.0	29.5	41.0
50-51	35.75370871906788	38.5	35.0	40.0	28.5	41.0
52-53	35.435597673896154	38.0	35.0	40.0	28.0	41.0
54-55	35.10213899596566	38.0	34.0	40.0	27.5	41.0
56-57	34.71603169146752	38.0	34.0	40.0	26.5	41.0
58-59	34.31972583853031	37.0	33.5	40.0	26.0	41.0
60-61	33.886457923080215	36.5	33.0	39.0	26.0	41.0
62-63	33.435145579989	36.0	32.5	39.0	24.5	40.0
64-65	32.914967387834054	35.0	32.0	38.5	23.0	40.0
66-67	32.38068301241839	35.0	31.0	38.0	22.0	40.0
68-69	31.876586989676046	35.0	31.0	37.0	20.5	39.5
70-71	31.3237626827924	34.5	30.5	36.5	19.5	39.0
72-73	30.780127736471364	34.0	30.0	36.0	18.0	39.0
74-75	30.264181640460865	34.0	29.5	35.5	16.0	37.5
76-77	28.978066488784936	32.5	28.0	34.5	13.0	36.0
78-79	29.929820908109548	34.0	29.5	35.0	14.0	36.5
80-81	29.97306303175992	34.0	30.0	35.0	14.0	36.0
82-83	29.651854020973673	34.0	30.0	35.0	8.5	36.0
84-85	29.171182751495092	34.0	29.0	35.0	6.0	35.5
86-87	28.662069451048172	33.5	29.0	35.0	2.0	35.0
88-89	28.129972174983532	33.0	27.5	35.0	2.0	35.0
90-91	27.661233995606693	33.0	27.0	35.0	2.0	35.0
92-93	27.22759301456477	33.0	26.0	34.0	2.0	35.0
94-95	26.729212074710325	32.0	25.0	34.0	2.0	35.0
96-97	25.954683661341036	32.0	24.5	34.0	2.0	35.0
98-99	24.941531754263494	32.0	20.5	34.0	2.0	35.0
100-101	21.322124885886637	27.5	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	30536.0
3	16242.0
4	9883.0
5	7628.0
6	6553.0
7	6860.0
8	7854.0
9	9467.0
10	11295.0
11	13428.0
12	15613.0
13	17815.0
14	20191.0
15	22832.0
16	25257.0
17	28808.0
18	30147.0
19	28414.0
20	29447.0
21	31111.0
22	33873.0
23	37284.0
24	42971.0
25	50928.0
26	60906.0
27	74192.0
28	90057.0
29	110431.0
30	136312.0
31	170690.0
32	220137.0
33	296487.0
34	420242.0
35	637400.0
36	986468.0
37	1176209.0
38	464949.0
39	11556.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.55548155149482	17.118404698246735	13.408121477324517	24.917992272933922
2	16.48738431673807	18.585641742385132	38.09313208692447	26.833841853952332
3	20.79945411061718	21.51851272600913	27.275211623970826	30.406821539402866
4	14.474180502230794	14.393732398496917	33.64096905996372	37.49111803930857
5	16.192358708456624	35.274068027715686	32.31247855002829	16.221094713799406
6	36.49954881763489	32.24120779133749	15.79238520003037	15.466858190997254
7	32.36965105373025	29.603158925795913	18.48260838246335	19.54458163801048
8	28.550705932057813	33.41591260463626	18.232667406413512	19.800714056892414
9	27.088045506478498	16.389811697522443	18.485187165149828	38.03695563084923
10-11	27.36092622907212	24.50111096348402	25.37731023628598	22.760652571157884
12-13	27.281674287652386	21.40826791454679	26.22486892180004	25.08518887600078
14-15	24.302409256886754	23.086591739316756	23.565786697726907	29.045212306069583
16-17	24.932773149790886	26.26095115646885	24.22645974591684	24.579815947823427
18-19	24.94396931895105	25.556272638571137	25.550640108716177	23.949117933761634
20-21	25.44629312413259	24.692288264876762	25.528499277495126	24.33291933349552
22-23	25.256474238065923	24.297854389334546	25.48938293069456	24.95628844190497
24-25	24.954303853693645	24.55292749940685	25.53373256626718	24.95903608063232
26-27	24.918075920411386	24.786221780481785	25.55200932367974	24.743692975427088
28-29	24.95399815909136	24.923140069470595	25.462034019420187	24.660827752017862
30-31	25.07654944328774	24.77250368114469	25.28896777122342	24.86197910434415
32-33	24.920071782802296	24.778405940169744	25.301990724189626	24.999531552838334
34-35	25.029512839175016	24.750107930813705	25.471096898030382	24.749282331980897
36-37	25.06357927648152	24.78604292138044	25.34676506136589	24.803612740772156
38-39	25.08258225314871	24.841034902882416	25.3111973529149	24.765185491053973
40-41	25.119985482619406	24.786933689489114	25.30337317572525	24.789707652166232
42-43	25.131286731483083	24.725836275191313	25.20144978916762	24.941427204157986
44-45	25.02197937681822	24.737010506035435	25.289990011524736	24.95102010562161
46-47	25.046663367433453	24.770839677120485	25.331969105965324	24.85052784948074
48-49	25.135717584611477	24.832053402301288	25.157729226014848	24.874499787072384
50-51	24.934720750262635	25.078975624349216	25.134429012381464	24.851874613006686
52-53	25.09452076395781	24.971940597549324	25.0650236070014	24.868515031491462
54-55	25.044592025520117	24.818424722752113	25.18234982280531	24.95463342892246
56-57	25.078687934578536	24.894439620947335	25.15888784998182	24.86798459449231
58-59	25.14702024742441	24.86206068773196	25.093313797418897	24.897605267424726
60-61	25.16885777803256	24.807682924544178	25.184348891791696	24.83911040563157
62-63	24.984519104237105	24.932855818242455	25.145065850048447	24.937559227471993
64-65	25.051239540014336	24.821244852507583	25.196941183826087	24.930574423651997
66-67	25.089243377617105	24.868062701882117	25.06376250265619	24.978931417844592
68-69	24.9988351848789	24.94037167533456	25.085931799192927	24.974861340593613
70-71	25.002553422184075	24.778789220541555	25.191233000029406	25.02742435724496
72-73	25.128118861048566	24.808517603167786	25.04993740658773	25.01342612919592
74-75	24.947368450359203	25.03698013091138	25.037574077427056	24.978077341302363
76-77	25.06728235123476	24.851202895027438	25.0385337820343	25.0429809717035
78-79	24.991074858130624	24.84640680193277	25.187149503691565	24.975368836245043
80-81	24.941207297987162	25.006316532948546	25.100152886541892	24.952323282522396
82-83	25.10508544524831	24.900082539300804	25.042828396191613	24.95200361925927
84-85	25.03570647611338	24.92324557232738	25.025343596264428	25.015704355294815
86-87	24.98489540450692	24.91338886574471	25.163401727635122	24.938314002113252
88-89	24.947889596251372	24.79696197791455	25.12579321853286	25.129355207301217
90-91	24.976954433539746	24.84679400296079	25.08897562329376	25.087275940205707
92-93	25.045933735708438	25.014381697442122	25.088315547109097	24.851369019740346
94-95	25.10718836432504	24.892839464761327	24.99136370686399	25.008608464049644
96-97	25.01407883162441	24.855253099406895	25.078677733572942	25.051990335395757
98-99	25.012147623824593	24.912897965711327	25.01696214598188	25.057992264482206
100-101	25.52764681463891	24.381366721783273	25.071596704135136	25.019389759442678
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	300.0
1	251.5
2	368.0
3	601.0
4	519.0
5	436.5
6	937.0
7	2256.5
8	3342.5
9	3120.0
10	2080.0
11	1295.5
12	1121.5
13	1115.0
14	1170.5
15	1247.0
16	1307.0
17	1351.0
18	1388.5
19	1441.0
20	1513.0
21	1604.0
22	1658.5
23	1753.0
24	1890.5
25	2121.0
26	2464.5
27	2964.5
28	3854.5
29	5192.5
30	7469.5
31	10860.5
32	15565.5
33	21630.5
34	28990.5
35	38181.5
36	49792.0
37	63272.0
38	77242.5
39	91432.0
40	107402.0
41	123011.0
42	139845.5
43	158913.0
44	176220.0
45	193175.5
46	210509.5
47	228300.5
48	248004.0
49	258429.0
50	256777.5
51	256198.0
52	258359.0
53	259291.0
54	257272.5
55	249610.5
56	234006.5
57	214005.5
58	196039.5
59	178163.0
60	155971.0
61	133116.5
62	109654.0
63	83845.0
64	61867.0
65	46539.0
66	35246.5
67	27085.0
68	20932.5
69	15790.0
70	11739.0
71	9052.5
72	6622.5
73	4657.0
74	3436.5
75	2473.0
76	1689.0
77	990.0
78	559.0
79	323.5
80	174.0
81	93.5
82	58.5
83	33.0
84	18.0
85	11.0
86	5.0
87	2.5
88	2.5
89	1.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3134047791353375
2	0.0
3	0.006863961659765293
4	0.05841787909892138
5	4.266786977691939E-4
6	0.06522618701549938
7	0.07578184697335466
8	0.1066325719468403
9	0.09353539105009895
10-11	0.09799696612894637
12-13	0.09708795499022072
14-15	0.06532821887800941
16-17	0.03712104670591987
18-19	0.039523433286837724
20-21	0.0293480739074289
22-23	0.04285338225421034
24-25	0.03505258258412573
26-27	0.021973952935113485
28-29	0.023866180203481215
30-31	0.0030795071230298346
32-33	0.006010604264226905
34-35	0.008273856487176543
36-37	0.01017535937940882
38-39	0.015703631202679244
40-41	0.01986838631786116
42-43	0.003190814609404407
44-45	0.01412677514570614
46-47	0.02489577445244601
48-49	0.002662104049125188
50-51	0.006687724806338887
52-53	0.0022818034706787328
54-55	0.003997793885620056
56-57	0.0035803908117154094
58-59	0.001094523616016628
60-61	0.005305656850521281
62-63	0.014200980136622519
64-65	0.0064651098335897425
66-67	0.03929154269022403
68-69	0.06218378238792774
70-71	0.012011932904589262
72-73	0.03203800482814773
74-75	0.05161884680620791
76-77	0.3022462036262866
78-79	0.07427919590729792
80-81	0.03406936645448368
82-83	0.028290652786870468
84-85	0.019376778253040135
86-87	0.11780042307975572
88-89	0.5250003107333995
90-91	0.13201995446410733
92-93	0.047277854837599595
94-95	0.007958485275781921
96-97	0.005352034969844019
98-99	0.009832161296420555
100-101	0.007151505999566272
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	5390473.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.28030585685373
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.6551263314633	30.181645072158826
2	16.384740022458278	14.838120792038865
3	6.581072235132337	8.939788910185207
4	3.217105857584907	5.8268614882128125
5	1.8972193945402247	4.295333723116811
6	1.158677376681937	3.147915960334501
7	0.7939752302219926	2.516600888705237
8	0.5367656806979344	1.9443931356371804
9	0.40895208410591705	1.6665727904202325
>10	2.1703610965599647	17.634048089995822
>50	0.14152367150235867	4.389211423737782
>100	0.052964529511388615	3.919309342313315
>500	0.0011886018724294551	0.3419994754912494
>1k	2.8690170864442086E-4	0.2533615609231673
>5k	4.098595837169748E-5	0.10483734672892156
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5649	0.10479599842165985	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.7102495458190776E-5
2	0.0	0.0	0.0	0.0	3.7102495458190776E-5
3	0.0	0.0	0.0	0.0	3.7102495458190776E-5
4	0.0	0.0	0.0	0.0	3.7102495458190776E-5
5	0.0	0.0	0.0	0.0	3.7102495458190776E-5
6	0.0	0.0	0.0	0.0	5.5653743187286164E-5
7	0.0	0.0	0.0	0.0	5.5653743187286164E-5
8	0.0	0.0	0.0	0.0	5.5653743187286164E-5
9	0.0	0.0	0.0	1.8551247729095388E-5	9.275623864547693E-5
10-11	0.0	0.0	0.0	4.6378119322738466E-5	1.1130748637457233E-4
12-13	0.0	0.0	0.0	5.5653743187286164E-5	1.6696122956185848E-4
14-15	0.0	0.0	0.0	5.5653743187286164E-5	1.9478810115550156E-4
16-17	0.0	0.0	0.0	1.0203186251002464E-4	2.0406372502004927E-4
18-19	0.0	0.0	0.0	1.2985873410366772E-4	2.6899309207188314E-4
20-21	0.0	0.0	0.0	1.2985873410366772E-4	2.782687159364308E-4
22-23	0.0	0.0	0.0	1.6696122956185848E-4	2.968199636655262E-4
24-25	0.0	0.0	0.0	1.7623685342640618E-4	3.153712113946216E-4
26-27	0.0	0.0	0.0	3.3392245912371697E-4	3.246468352591693E-4
28-29	0.0	0.0	0.0	8.997355148611263E-4	3.5247370685281235E-4
30-31	0.0	0.0	0.0	0.00321864148099805	3.5247370685281235E-4
32-33	0.0	0.0	0.0	0.007698767807574586	3.895762023110031E-4
34-35	0.0	0.0	0.0	0.013727923319530586	4.452299454982893E-4
36-37	0.0	0.0	0.0	0.023541533368222047	4.452299454982893E-4
38-39	0.0	0.0	0.0	0.03866080026743479	4.8233244095648013E-4
40-41	0.0	0.0	0.0	0.05467052705764411	5.194349364146709E-4
42-43	0.0	0.0	0.0	0.07026285077394878	5.287105602792186E-4
44-45	0.0	0.0	0.0	0.08785870924499575	5.379861841437663E-4
46-47	0.0	0.0	0.0	0.10582559267062464	5.47261808008314E-4
48-49	0.0	0.0	0.0	0.1236718929860144	5.936399273310524E-4
50-51	0.0	0.0	0.0	0.14211183322873522	6.121911750601478E-4
52-53	0.0	0.0	0.0	0.15946652547930396	6.214667989246955E-4
54-55	0.0	0.0	0.0	0.1773499282901519	6.307424227892432E-4
56-57	0.0	0.0	0.0	0.19749658332394948	6.307424227892432E-4
58-59	0.0	0.0	0.0	0.21841311513850453	6.678449182474339E-4
60-61	0.0	0.0	0.0	0.2387823851450513	6.95671789841077E-4
62-63	0.0	0.0	0.0	0.2593649945004826	7.977036523511016E-4
64-65	0.0	0.0	0.0	0.2796693351399775	8.255305239447448E-4
66-67	0.0	1.8551247729095388E-5	0.0	0.3013650193591546	8.533573955383879E-4
68-69	0.0	1.8551247729095388E-5	0.0	0.3241274003227546	8.719086432674832E-4
70-71	0.0	1.8551247729095388E-5	0.0	0.34615700700105534	8.719086432674832E-4
72-73	0.0	1.8551247729095388E-5	0.0	0.36968926474541286	0.001001767377371151
74-75	0.0	1.8551247729095388E-5	0.0	0.39270208755335567	0.001029594248964794
76-77	0.0	1.8551247729095388E-5	0.0	0.41638275527954594	0.0010574211205584372
78-79	0.0	1.8551247729095388E-5	0.0	0.4427162514309969	0.0010574211205584372
80-81	0.0	1.8551247729095388E-5	0.0	0.4693094650506551	0.0011037992398811756
82-83	0.0	1.8551247729095388E-5	0.0	0.4958748517987197	0.0012058311023912002
84-85	0.0	3.7102495458190776E-5	0.0	0.5226999560149916	0.001242933597849391
86-87	0.0	3.7102495458190776E-5	0.0	0.5494044771210245	0.0012800360933075817
88-89	9.275623864547694E-6	3.7102495458190776E-5	0.0	0.5777229567794886	0.0013171385887657725
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	10990	0.0	34.278065	1
TCAACGC	13570	0.0	27.40639	4
CAACGCA	13845	0.0	26.81426	5
TATCAAC	14040	0.0	26.657095	2
ATCAACG	14265	0.0	26.070887	3
GTACATG	18435	0.0	25.808329	1
AACGCAG	14775	0.0	25.351377	6
TACATGG	18910	0.0	24.714811	2
GGTATCA	7355	0.0	22.922295	1
ACATGGG	19880	0.0	22.721132	3
GAGTACA	14180	0.0	21.49519	1
ACGCAGA	17460	0.0	21.290295	7
CATGGGG	15400	0.0	20.541084	4
AGTACAT	13715	0.0	20.431955	2
CGCAGAG	18605	0.0	19.877966	8
GTACCTG	5190	0.0	19.545633	1
AGAGTAC	15080	0.0	18.13281	10-11
GTGTAGC	3075	0.0	17.191565	1
GCAGAGT	21040	0.0	17.05784	9
TACCTGG	5635	0.0	16.26736	2
>>END_MODULE