FastQCFastQC Report
Mon 15 Aug 2016
SRR2921452_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921452_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4265009
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64700.042.334341
GGTATCA35800.035.4900131
GTACCTG55650.034.908191
TACCTGG57900.033.182962
CAACGCA81100.032.736895
TCAACGC81300.032.7127534
TATCAAC83400.031.8890512
ATCAACG85350.031.0530283
AACGCAG87150.030.6134996
ACCTGGG72900.025.6632883
GTACATG129850.025.2062231
ACGCAGA107000.025.0126137
TACATGG130000.024.0911222
CGCAGAG113650.023.302198
ACATGGG133950.022.2168063
GTGTAGC25250.020.601421
GAGTACA99850.019.271181
GCAGAGT134750.019.1771289
AGTACAT95200.018.68852
CATGGGG102800.018.4219934
Produced by FastQC (version 0.11.5)