##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921452_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4265009
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.086728304676498	31.0	31.0	34.0	26.0	34.0
2	30.178615566813576	31.0	31.0	34.0	26.0	34.0
3	30.31053533532989	31.0	31.0	34.0	26.0	34.0
4	33.8082705100974	37.0	35.0	37.0	30.0	37.0
5	33.78271980199807	37.0	35.0	37.0	29.0	37.0
6	33.880210100377276	37.0	35.0	37.0	30.0	37.0
7	33.75075152244696	37.0	35.0	37.0	29.0	37.0
8	33.72649764631212	37.0	35.0	37.0	29.0	37.0
9	35.14146464872641	39.0	35.0	39.0	29.0	39.0
10-11	35.002864706733334	39.0	35.0	39.0	28.0	39.0
12-13	34.82046520886591	38.0	35.0	39.0	27.0	39.0
14-15	35.78104758981751	39.0	36.0	41.0	27.0	41.0
16-17	35.62164206453023	39.0	36.0	41.0	27.0	41.0
18-19	35.42677788018736	39.0	36.0	40.0	27.0	41.0
20-21	35.30146032517165	39.0	35.5	40.0	26.0	41.0
22-23	35.333726142195715	39.0	35.5	40.0	26.5	41.0
24-25	35.186147907308055	39.0	35.0	40.5	25.0	41.0
26-27	34.89556094723364	39.0	34.5	40.0	24.0	41.0
28-29	34.64511493410682	39.0	34.0	40.0	23.5	41.0
30-31	34.37468666537397	38.0	34.0	40.0	22.0	41.0
32-33	34.08782068220724	38.0	34.0	40.0	19.5	41.0
34-35	33.65283250281535	38.0	33.0	40.0	17.0	41.0
36-37	33.255655380797556	38.0	33.0	40.0	15.0	41.0
38-39	33.00368697932407	38.0	32.0	40.0	9.5	41.0
40-41	32.71883166014421	38.0	31.5	40.0	9.0	41.0
42-43	32.41466278265767	37.5	31.0	40.0	8.5	41.0
44-45	32.08026559850167	37.0	31.0	40.0	7.5	41.0
46-47	31.778051933770833	37.0	30.0	40.0	4.5	41.0
48-49	31.542134143210482	37.0	30.0	40.0	2.0	41.0
50-51	30.83186190228438	36.0	29.0	38.5	2.0	39.5
52-53	31.388642321739532	36.5	30.5	39.0	2.0	40.0
54-55	31.80562831168703	37.0	31.0	39.5	2.0	40.5
56-57	31.640781297296208	37.0	31.0	40.0	2.0	41.0
58-59	31.293522944500232	36.0	30.5	39.0	2.0	41.0
60-61	30.82379286890133	35.5	29.5	39.0	2.0	40.0
62-63	30.31518456350268	35.0	29.0	39.0	2.0	40.0
64-65	29.750525497132596	35.0	28.0	38.0	2.0	40.0
66-67	29.242260684561273	34.0	28.0	38.0	2.0	40.0
68-69	28.701506843244644	34.0	26.5	37.0	2.0	39.5
70-71	28.15650353844505	34.0	26.0	36.5	2.0	39.0
72-73	27.57208753838503	33.5	25.5	36.0	2.0	39.0
74-75	26.948058257321378	33.0	24.5	35.0	2.0	38.0
76-77	26.329032248232068	32.5	22.5	35.0	2.0	37.0
78-79	25.728895531052807	32.0	20.5	35.0	2.0	36.5
80-81	25.10179169610193	31.5	18.5	34.5	2.0	36.0
82-83	24.431776931772006	31.0	13.5	34.0	2.0	35.5
84-85	23.7723215824398	31.0	7.0	34.0	2.0	35.0
86-87	23.10106660970704	30.0	2.0	34.0	2.0	35.0
88-89	22.41325985478577	30.0	2.0	34.0	2.0	35.0
90-91	21.626666790152143	29.0	2.0	34.0	2.0	35.0
92-93	20.767062859656335	29.0	2.0	34.0	2.0	35.0
94-95	19.820364388445604	27.0	2.0	33.0	2.0	34.0
96-97	18.90186105117246	25.5	2.0	33.0	2.0	34.0
98	18.273628496446314	25.0	2.0	32.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	152150.0
3	27054.0
4	20733.0
5	17287.0
6	16100.0
7	17066.0
8	19517.0
9	22645.0
10	25156.0
11	27523.0
12	30136.0
13	33270.0
14	28758.0
15	29298.0
16	31941.0
17	36638.0
18	41268.0
19	41119.0
20	43058.0
21	46718.0
22	51204.0
23	56170.0
24	61812.0
25	69044.0
26	77541.0
27	87598.0
28	100548.0
29	116413.0
30	135934.0
31	162600.0
32	198253.0
33	251316.0
34	327800.0
35	443068.0
36	577575.0
37	585113.0
38	245333.0
39	10251.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.36929086864011	16.83608170548398	12.900391117215987	24.894236308659927
2	16.052622072765843	18.268117923959583	39.3002223236599	26.37903767961467
3	20.62244066855086	21.886506435158598	27.205622928607088	30.28542996768346
4	13.720802741204844	14.25245423173615	34.43900141287369	37.58774161418532
5	15.600202136604555	35.45350764252789	32.76736178886203	16.178928432005527
6	35.81398655144698	32.66867114013343	16.520577524841254	14.996764783578332
7	32.00352116722988	29.678476525199965	18.80908926506072	19.50891304250944
8	28.268034245438145	33.99055314430867	18.377469000599397	19.363943609653784
9	26.948776204631613	16.56257232573294	18.54057193694732	37.94807953268812
10-11	26.944493134848447	24.89956260709853	25.826324357819168	22.32961990023385
12-13	26.86595652731587	21.77921821203304	26.591675813656067	24.763149446995023
14-15	23.87382248510442	23.453743536807266	24.005199749134498	28.667234228953813
16-17	24.53269272054119	26.6029907431389	24.525435058167407	24.3388814781525
18-19	24.438858399708828	25.948650483818696	25.944955733964402	23.667535382508067
20-21	24.95514393186829	25.13687949594603	26.0010796195356	23.906896952650083
22-23	24.66349074999994	24.8376858442812	25.865794795480056	24.6330286102388
24-25	24.38719958256652	25.031286395216036	26.056112331649395	24.52540169056805
26-27	24.48248027454316	25.28243334408823	25.903029947496552	24.332056433872058
28-29	24.434700398954956	25.366303159773928	25.853193868830594	24.345802572440526
30-31	24.629005425938967	25.30126869546746	25.74830253934976	24.321423339243818
32-33	24.455373279740396	25.256722609648886	25.714653264992805	24.573250845617913
34-35	24.537009797576374	25.306530227041122	25.853610605764636	24.302849369617864
36-37	24.62760141251546	25.236272268676064	25.72627041010309	24.409855908705385
38-39	24.588358909727518	25.33970232462524	25.750388632001208	24.32155013364603
40-41	24.734209356005113	25.203526580007345	25.81454538039542	24.24771868359212
42-43	24.57523579680702	25.137188727003622	25.726539337176472	24.56103613901288
44-45	24.578627425983655	25.282872857613807	25.721783230682917	24.416716485719625
46-47	24.64803276164293	25.260092841619663	25.67840202991935	24.413472366818056
48-49	24.638805199358043	25.320942937299918	25.562406997816478	24.477844865525558
50-51	24.45943920794095	25.487308214139453	25.585036119856948	24.468216458062653
52-53	24.53393944296051	25.429049258866144	25.570232210228887	24.466779087944456
54-55	24.58151474617419	25.282200249608305	25.685562370722558	24.450722633494944
56-57	24.514715507647754	25.37976596342582	25.669639990409017	24.435878538517414
58-59	24.534184355690655	25.403399039713737	25.67111249749232	24.39130410710329
60-61	24.66876973838765	25.296309971533603	25.64306232564891	24.391857964429846
62-63	24.477914495287102	25.439711725944527	25.632643817712992	24.449729961055382
64-65	24.522410468903942	25.283183191676923	25.677688054192416	24.51671828522672
66-67	24.540510516627435	25.400432471784658	25.565968921630756	24.493088089957148
68-69	24.501822966037558	25.286046542043806	25.661373822271326	24.550756669647306
70-71	24.4535061401985	25.20266040918954	25.647663603682645	24.696169846929312
72-73	24.532820382268806	25.257525471124247	25.58077318989167	24.628880956715278
74-75	24.43830522769768	25.380202509387185	25.542646090322773	24.63884617259236
76-77	24.50977217662814	25.2680609150731	25.54902372429615	24.673143184002615
78-79	24.49779611564868	25.161946086926424	25.685198635695684	24.655059161729213
80-81	24.471939062039727	25.343534242517503	25.573659859127236	24.61086683631553
82-83	24.62887500537265	25.240409157468395	25.520469943482667	24.61024589367629
84-85	24.593360677863068	25.265052921117864	25.459512763847776	24.682073637171296
86-87	24.527137758545447	25.250394579170294	25.626697202528742	24.595770459755514
88-89	24.57391624019727	25.01561689900962	25.631908895515203	24.77855796527791
90-91	24.53705796753366	25.125692864846027	25.574259570600393	24.762989597019917
92-93	24.59628752164427	25.20119696299718	25.618530558157342	24.583984957201203
94-95	24.689311808141106	25.108492139986122	25.475854803148195	24.726341248724573
96-97	24.674274703820004	25.0585107591629	25.513292912177526	24.753921624839574
98	24.609942386963052	25.089489864800434	25.410718628984423	24.889849119252087
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	458.0
1	332.5
2	356.5
3	506.5
4	412.0
5	367.5
6	620.0
7	1222.0
8	1780.5
9	1835.0
10	1554.0
11	1425.5
12	1590.0
13	1881.5
14	2198.0
15	2526.5
16	2777.0
17	2924.0
18	2986.0
19	2889.5
20	2756.5
21	2645.0
22	2607.0
23	2761.5
24	3168.5
25	3866.0
26	4964.0
27	6413.5
28	8346.0
29	11229.0
30	15053.0
31	20220.0
32	26829.5
33	34235.0
34	42775.0
35	52384.0
36	62630.0
37	73797.0
38	85899.5
39	97398.5
40	109225.5
41	121828.5
42	134610.0
43	147224.5
44	158343.0
45	169306.5
46	179999.5
47	189657.0
48	195351.0
49	196320.5
50	196272.0
51	194922.0
52	194180.0
53	193551.0
54	188911.5
55	181458.0
56	170297.0
57	157605.5
58	144677.0
59	129179.5
60	112489.0
61	94188.5
62	75755.5
63	58722.0
64	43858.0
65	32974.5
66	24931.5
67	18843.0
68	14365.0
69	10756.0
70	8108.5
71	6337.0
72	4909.5
73	3578.0
74	2661.0
75	1971.0
76	1290.0
77	808.0
78	526.5
79	327.0
80	196.0
81	117.5
82	63.0
83	35.5
84	30.5
85	19.5
86	14.0
87	11.0
88	10.5
89	11.5
90	11.5
91	10.5
92	12.0
93	9.5
94	3.0
95	3.5
96	5.5
97	3.5
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4507141719982302
2	0.04349346038894643
3	0.051043268607405046
4	0.04844069496688049
5	0.05967162085707205
6	0.05962472763832386
7	0.03868690546725693
8	0.017186364671211714
9	0.01050408099959461
10-11	0.012579105929202026
12-13	0.009378643749638043
14-15	0.013423183866669448
16-17	0.012907358460439358
18-19	0.051664603755818564
20-21	0.07790135964543099
22-23	0.09336439852764672
24-25	0.06501744779436573
26-27	0.07934332612193784
28-29	0.07913230663757098
30-31	0.05262591474015647
32-33	0.02961306763948212
34-35	0.37512230337614766
36-37	0.5907842163990744
38-39	0.5441254637446251
40-41	0.5391665058620041
42-43	0.5146296291520135
44-45	0.4998348186369595
46-47	0.4900224126139007
48-49	0.4803037930283383
50-51	0.49432486543404713
52-53	0.5723786280404097
54-55	0.543093812932165
56-57	0.5027304748946603
58-59	0.48172231289547107
60-61	0.49373870019969474
62-63	0.5052040921836273
64-65	0.5240082729016515
66-67	0.5473610958382503
68-69	0.504395184160221
70-71	0.45536832395898813
72-73	0.4391549935768013
74-75	0.4219451822962156
76-77	0.4346884144910363
78-79	0.4291315680693757
80-81	0.4266813973897827
82-83	0.44470011669377485
84-85	0.41271894150750915
86-87	0.4164703990073643
88-89	0.4409721058032937
90-91	0.4255325135304521
92-93	0.420327366249403
94-95	0.43113625317086085
96-97	0.42533321735077234
98	0.4192253756088205
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4265009.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.41171094550244
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.22782115879374	46.654648087960055
2	12.881419108783062	15.563771355353484
3	4.3330099239237025	7.852936291442167
4	1.897136438941359	4.584370326940209
5	0.9926529075727285	2.998393026074812
6	0.6132202968343164	2.222741239096196
7	0.39657219191218285	1.6770323238776177
8	0.29304266508040205	1.4162567022029544
9	0.20936850225464565	1.1383478495370387
>10	1.0798502140296584	11.510490896327639
>50	0.057689347418597076	2.3586480012683206
>100	0.01786637991373139	1.719115127639892
>500	2.3390970569115685E-4	0.09724404199506613
>1k	1.1695483625004646E-4	0.2060047302845322
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3446609374095108E-5
2	0.0	0.0	0.0	0.0	4.6893218748190216E-5
3	0.0	0.0	0.0	0.0	4.6893218748190216E-5
4	0.0	0.0	0.0	9.378643749638043E-5	4.6893218748190216E-5
5	0.0	0.0	0.0	9.378643749638043E-5	4.6893218748190216E-5
6	0.0	0.0	0.0	9.378643749638043E-5	4.6893218748190216E-5
7	0.0	0.0	0.0	1.4067965624457065E-4	7.033982812228532E-5
8	0.0	0.0	0.0	1.4067965624457065E-4	7.033982812228532E-5
9	0.0	0.0	0.0	1.4067965624457065E-4	9.378643749638043E-5
10-11	0.0	0.0	0.0	1.4067965624457065E-4	9.378643749638043E-5
12-13	0.0	0.0	0.0	2.2274278905390352E-4	1.2895635155752309E-4
14-15	0.0	1.0550974218342799E-4	0.0	2.3446609374095108E-4	1.5240296093161818E-4
16-17	0.0	1.8757287499276086E-4	0.0	2.6963600780209373E-4	1.6412626561866574E-4
18-19	0.0	1.8757287499276086E-4	0.0	3.516991406114266E-4	1.6412626561866574E-4
20-21	0.0	1.8757287499276086E-4	0.0	3.516991406114266E-4	1.6412626561866574E-4
22-23	0.0	1.8757287499276086E-4	0.0	4.220389687337119E-4	1.758495703057133E-4
24-25	0.0	2.2274278905390352E-4	0.0	4.220389687337119E-4	2.1101948436685596E-4
26-27	0.0	2.5791270311504617E-4	0.0	4.572088827948546E-4	2.1101948436685596E-4
28-29	0.0	2.813593124891413E-4	0.0	7.502914999710435E-4	2.1101948436685596E-4
30-31	0.0	2.813593124891413E-4	0.0	0.001524029609316182	2.1101948436685596E-4
32-33	0.0	2.813593124891413E-4	0.0	0.0037162875857940742	2.6963600780209373E-4
34-35	0.0	3.516991406114266E-4	0.0	0.006787793413800534	3.048059218632364E-4
36-37	0.0	4.337622734207595E-4	0.0	0.011770197905795744	3.165292265502839E-4
38-39	0.0	5.158254062300923E-4	0.0	0.019108986639887512	3.282525312373315E-4
40-41	0.0	5.392720156041875E-4	0.0	0.027819402022363843	3.282525312373315E-4
42-43	0.0	5.861652343523777E-4	0.0	0.03651809410015313	3.282525312373315E-4
44-45	0.0	6.096118437264728E-4	0.0	0.045298849310751746	3.3997583592437904E-4
46-47	0.0	6.096118437264728E-4	0.0	0.054525090099458176	3.516991406114266E-4
48-49	0.0	6.56505062474663E-4	0.0	0.06424370968502059	3.751457499855217E-4
50-51	0.0	7.151215859099008E-4	0.0	0.07457194111430948	3.751457499855217E-4
52-53	0.0	7.502914999710435E-4	0.0	0.08450158018423877	3.751457499855217E-4
54-55	0.0	7.62014804658091E-4	0.0	0.09550976328537641	3.751457499855217E-4
56-57	0.0	7.971847187192337E-4	0.0	0.10626003368339902	3.868690546725693E-4
58-59	1.1723304687047554E-5	7.971847187192337E-4	0.0	0.1171978769564144	3.9859235935961685E-4
60-61	2.3446609374095108E-5	8.089080234062811E-4	0.0	0.1275964482138256	3.9859235935961685E-4
62-63	2.3446609374095108E-5	8.206313280933287E-4	0.0	0.13866324783839845	3.9859235935961685E-4
64-65	2.3446609374095108E-5	8.206313280933287E-4	0.0	0.1501872563457662	4.220389687337119E-4
66-67	2.3446609374095108E-5	8.440779374674238E-4	0.0	0.1630594448921444	4.220389687337119E-4
68-69	2.3446609374095108E-5	8.440779374674238E-4	0.0	0.17516961863386454	4.220389687337119E-4
70-71	2.3446609374095108E-5	8.67524546841519E-4	0.0	0.188346613102106	4.220389687337119E-4
72-73	2.3446609374095108E-5	8.909711562156141E-4	0.0	0.2006443597188189	5.392720156041875E-4
74-75	2.3446609374095108E-5	8.909711562156141E-4	0.0	0.21239111101524055	5.627186249782826E-4
76-77	2.3446609374095108E-5	8.909711562156141E-4	0.0	0.2254508724366115	5.627186249782826E-4
78-79	2.3446609374095108E-5	8.909711562156141E-4	0.0	0.23927264866264059	5.744419296653301E-4
80-81	2.3446609374095108E-5	8.909711562156141E-4	0.0	0.2534461240292811	6.096118437264728E-4
82-83	3.516991406114266E-5	8.909711562156141E-4	0.0	0.2676195993959215	6.33058453100568E-4
84-85	4.6893218748190216E-5	9.026944609026616E-4	0.0	0.282027540856303	6.447817577876155E-4
86	4.6893218748190216E-5	9.144177655897092E-4	0.0	0.29364533580116714	6.56505062474663E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6470	0.0	42.33434	1
GGTATCA	3580	0.0	35.490013	1
GTACCTG	5565	0.0	34.90819	1
TACCTGG	5790	0.0	33.18296	2
CAACGCA	8110	0.0	32.73689	5
TCAACGC	8130	0.0	32.712753	4
TATCAAC	8340	0.0	31.889051	2
ATCAACG	8535	0.0	31.053028	3
AACGCAG	8715	0.0	30.613499	6
ACCTGGG	7290	0.0	25.663288	3
GTACATG	12985	0.0	25.206223	1
ACGCAGA	10700	0.0	25.012613	7
TACATGG	13000	0.0	24.091122	2
CGCAGAG	11365	0.0	23.30219	8
ACATGGG	13395	0.0	22.216806	3
GTGTAGC	2525	0.0	20.60142	1
GAGTACA	9985	0.0	19.27118	1
GCAGAGT	13475	0.0	19.177128	9
AGTACAT	9520	0.0	18.6885	2
CATGGGG	10280	0.0	18.421993	4
>>END_MODULE