FastQCFastQC Report
Mon 15 Aug 2016
SRR2921452_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921452_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4265009
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64750.039.580291
GGTATCA37700.037.6540261
GTACCTG54100.033.459761
TCAACGC80650.030.9804064
TACCTGG57300.030.2564872
CAACGCA83450.029.9251085
ATCAACG84400.029.546933
TATCAAC86600.028.9598482
AACGCAG91050.027.324566
GTACATG127200.024.679411
TACATGG130350.023.211792
ACCTGGG72450.022.9469623
ACGCAGA109750.022.4538147
ACATGGG132050.021.9425453
GTGTAGC27100.021.269271
CGCAGAG116200.021.1665928
GAGTACA100600.019.7931441
AGTACAT95050.019.38922
GTATAGG10500.019.0544531
TATCACG3506.366463E-1118.9994162
Produced by FastQC (version 0.11.5)