##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921452_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4265009
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.902068905364562	33.0	31.0	34.0	30.0	34.0
2	32.010443354281314	34.0	31.0	34.0	30.0	34.0
3	32.139766879741636	34.0	31.0	34.0	30.0	34.0
4	35.70243954936554	37.0	35.0	37.0	35.0	37.0
5	35.5039764277168	37.0	35.0	37.0	33.0	37.0
6	35.54225301752001	37.0	35.0	37.0	33.0	37.0
7	35.47315492183018	37.0	35.0	37.0	33.0	37.0
8	35.420877892637506	37.0	35.0	37.0	33.0	37.0
9	37.03866580351882	39.0	37.0	39.0	33.0	39.0
10-11	36.94427526413192	39.0	37.0	39.0	33.0	39.0
12-13	36.82051854990224	39.0	37.0	39.0	33.0	39.0
14-15	38.09943507739374	40.0	38.0	41.0	33.0	41.0
16-17	37.994092978467336	40.0	38.0	41.0	33.0	41.0
18-19	37.8788494936353	40.0	38.0	41.0	33.0	41.0
20-21	37.759011059531176	40.0	38.0	41.0	32.5	41.0
22-23	37.72639436399783	40.0	38.0	41.0	32.5	41.0
24-25	37.78802084591146	40.0	38.0	41.0	33.0	41.0
26-27	37.675881223228366	40.0	38.0	41.0	32.0	41.0
28-29	37.53342841714988	40.0	38.0	41.0	32.0	41.0
30-31	37.38860668289328	40.0	37.0	41.0	32.0	41.0
32-33	37.22746774977497	40.0	37.0	41.0	31.0	41.0
34-35	37.080296430793	40.0	37.0	41.0	31.0	41.0
36-37	36.98095971192558	40.0	37.0	41.0	31.0	41.0
38-39	36.944938217011966	40.0	37.0	41.0	31.0	41.0
40-41	36.77664795080151	40.0	36.0	41.0	30.5	41.0
42-43	36.53467378380679	39.0	36.0	41.0	30.0	41.0
44-45	36.325879265436484	39.0	35.5	40.5	30.0	41.0
46-47	36.054378666023915	39.0	35.0	40.0	29.5	41.0
48-49	35.7161408100194	39.0	35.0	40.0	28.0	41.0
50-51	35.426126885078084	38.0	35.0	40.0	27.5	41.0
52-53	35.098910928441185	38.0	34.0	40.0	27.0	41.0
54-55	34.75530954330929	38.0	34.0	40.0	26.0	41.0
56-57	34.35880404941701	37.5	33.5	40.0	25.5	41.0
58-59	33.95084934170127	37.0	33.0	40.0	24.5	41.0
60-61	33.50721381830613	36.5	32.5	39.0	23.0	40.5
62-63	33.04308197239443	36.0	32.0	39.0	22.5	40.0
64-65	32.498863191144494	35.0	31.0	39.0	20.5	40.0
66-67	31.94576154470014	35.0	31.0	38.0	19.5	40.0
68-69	31.418139211429565	35.0	30.5	37.5	18.0	39.5
70-71	30.838594010000918	34.0	30.0	37.0	14.0	39.0
72-73	30.2567007478765	34.0	29.0	36.0	9.5	39.0
74-75	29.70529347065856	34.0	29.0	35.5	7.5	38.0
76-77	28.484459001141616	32.0	27.0	34.5	7.0	36.0
78-79	29.415289979458425	34.0	29.0	35.0	7.0	37.0
80-81	29.421911067479577	34.0	29.0	35.0	4.5	36.5
82-83	29.049437058632233	34.0	29.0	35.0	2.0	36.0
84-85	28.514397390486163	33.5	28.5	35.0	2.0	36.0
86-87	27.948834222858615	33.0	27.0	35.0	2.0	35.0
88-89	27.36039806715531	33.0	26.0	35.0	2.0	35.0
90-91	26.833350058581352	32.0	25.0	34.0	2.0	35.0
92-93	26.341420381527918	32.0	25.0	34.0	2.0	35.0
94-95	25.77626026111551	32.0	23.5	34.0	2.0	35.0
96-97	24.93181620484271	31.5	19.0	34.0	2.0	35.0
98-99	23.83944992847612	31.0	4.5	34.0	2.0	35.0
100-101	20.28048850541699	25.5	2.0	32.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25281.0
3	13476.0
4	8345.0
5	6321.0
6	5576.0
7	5855.0
8	6794.0
9	8135.0
10	9806.0
11	11613.0
12	13839.0
13	16379.0
14	18231.0
15	21123.0
16	23593.0
17	27551.0
18	29103.0
19	26833.0
20	27932.0
21	29552.0
22	31698.0
23	35101.0
24	39380.0
25	45678.0
26	54841.0
27	66456.0
28	80007.0
29	97133.0
30	118106.0
31	146199.0
32	186103.0
33	245078.0
34	337170.0
35	490245.0
36	728278.0
37	857392.0
38	360430.0
39	10376.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.63006924163454	17.28719638876725	13.257696376302718	24.825037993295496
2	16.22376412335824	18.577897490954882	38.88205159707752	26.316286788609354
3	20.640126469282734	22.010405765776245	27.391564009246967	29.95790375569406
4	14.074994885923244	14.528458664184384	34.30351435601106	37.09303209388131
5	15.633044430402451	35.39559928112291	32.87638760745934	16.09496868101529
6	35.94564387078853	32.80676087319656	16.3123755398357	14.935219716179212
7	32.15439772124693	29.585239724259615	18.73270170202582	19.527660852467633
8	28.27909059587056	34.103382429840565	18.28832376844874	19.329203205840134
9	26.82146969792617	16.581757951846445	18.56570958286115	38.03106276736624
10-11	26.99201809308896	24.950772393207394	25.828109787801175	22.22909972590247
12-13	26.934985210363916	21.736622912624277	26.609505262970153	24.718886614041658
14-15	23.822965245450575	23.502113594997756	23.996705147280668	28.678216012271
16-17	24.52852979493739	26.657775800292317	24.59989049035763	24.21380391441267
18-19	24.408558997974172	25.975332612632563	26.00140309135129	23.614705298041983
20-21	24.939136328643304	25.11603117617777	26.102561486643765	23.842271008535164
22-23	24.656583152633917	24.80278460641539	25.981731737807056	24.558900503143636
24-25	24.374829957501102	25.009510652869377	26.122620577722323	24.493038811907198
26-27	24.403753353847076	25.291999569431816	26.00051405526047	24.303733021460637
28-29	24.37879242174069	25.39029250259175	25.95498349149223	24.275931584175332
30-31	24.564436442129516	25.29043627752922	25.863500870013368	24.281626410327895
32-33	24.34456551214592	25.242394957495517	25.838099008730918	24.574940521627642
34-35	24.461869173451547	25.284636582825136	26.010445067258658	24.243049176464655
36-37	24.522314389114996	25.30109590785327	25.841891980776854	24.334697722254877
38-39	24.53666525143865	25.309424304706212	25.898262341532863	24.255648102322276
40-41	24.583626034214667	25.233577355287924	25.88974325691627	24.29305335358114
42-43	24.49352908832774	25.222920826147664	25.809154121300725	24.474395964223874
44-45	24.48012567313167	25.292866797548818	25.83121970848861	24.3957878208309
46-47	24.45887976352729	25.30734334873006	25.86145900746048	24.37231788028217
48-49	24.53631066162786	25.365252037123987	25.72317388635952	24.375263414888636
50-51	24.44515518771986	25.54229998504	25.613758603758384	24.398786223481757
52-53	24.52188378900869	25.453742923364242	25.601752907150555	24.422620380476516
54-55	24.590725906907355	25.299908847640733	25.692127777435836	24.417237468016072
56-57	24.459976285265235	25.449797988632216	25.70374542276779	24.386480303334764
58-59	24.59963087669657	25.371148522598226	25.664655828217338	24.364564772487878
60-61	24.63837712137883	25.367783824088885	25.659909948197644	24.333929106334637
62-63	24.461998334111872	25.440777958499122	25.689676277415398	24.407547429973604
64-65	24.545849223994097	25.305048514071537	25.689824384676918	24.459277877257446
66-67	24.551084513576768	25.446112549977883	25.58060658828452	24.422196348160828
68-69	24.54612444492825	25.40707216777337	25.650355940346913	24.39644744695147
70-71	24.48527181463442	25.272370390667326	25.66505790475909	24.57729988993916
72-73	24.59919430761632	25.268724397008956	25.58387602019109	24.548205275183633
74-75	24.45320399732436	25.45307462534614	25.50460056372403	24.589120813605476
76-77	24.500663117498778	25.316168309567704	25.62978526280146	24.55338331013206
78-79	24.426399714683924	25.297716532846543	25.742103361380032	24.533780391089504
80-81	24.43443673878211	25.50533838336515	25.60376451515719	24.456460362695545
82-83	24.579371271174207	25.386275775122595	25.555748001413285	24.47860495228991
84-85	24.514398949392618	25.44498381877023	25.51058815252568	24.530029079311475
86-87	24.443742586775077	25.447058688211584	25.638743294501122	24.470455430512217
88-89	24.51758694252475	25.217597877802046	25.67077556568849	24.594039613984712
90-91	24.493438630767127	25.34183751205819	25.592462401571243	24.57226145560344
92-93	24.583906138631374	25.43755170201903	25.64912753557609	24.329414623773506
94-95	24.59093273655427	25.375412854190525	25.48096611896133	24.552688290293872
96-97	24.55947810562396	25.353588738996773	25.571525411329237	24.515407744050034
98-99	24.4710422890644	25.362335577668144	25.56339830597998	24.60322382728748
100-101	25.095800511240885	24.79192369302427	25.640560585257443	24.471715210477402
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	149.0
1	134.0
2	250.0
3	371.5
4	266.5
5	210.0
6	415.5
7	962.0
8	1491.0
9	1459.5
10	1056.0
11	736.0
12	689.5
13	743.5
14	810.0
15	885.0
16	941.0
17	1085.5
18	1207.0
19	1271.0
20	1381.0
21	1495.0
22	1667.0
23	1979.5
24	2417.0
25	3041.5
26	3817.5
27	4841.5
28	6404.0
29	8740.0
30	12147.0
31	16454.5
32	21806.0
33	28484.0
34	36027.5
35	44560.5
36	54724.0
37	65742.0
38	76841.5
39	88310.5
40	100851.5
41	112573.0
42	124251.0
43	136924.0
44	149409.5
45	161119.0
46	171228.5
47	181076.0
48	190575.5
49	195476.5
50	194619.5
51	192918.0
52	192201.5
53	190213.5
54	186604.5
55	180157.5
56	169149.5
57	155514.5
58	142023.0
59	127857.0
60	111529.0
61	94036.0
62	75937.5
63	58248.5
64	43367.0
65	32330.0
66	24338.0
67	18946.5
68	14547.0
69	10840.5
70	8217.5
71	6252.5
72	4636.5
73	3481.5
74	2629.5
75	1877.5
76	1278.0
77	780.5
78	474.5
79	266.0
80	151.0
81	83.5
82	36.0
83	15.5
84	10.5
85	7.0
86	3.0
87	1.0
88	0.5
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.303469465128913
2	0.0
3	0.006049225218516538
4	0.05310657023232542
5	5.627186249782826E-4
6	0.06098463098202138
7	0.07101977979413408
8	0.10046872116799754
9	0.08829993090284217
10-11	0.0922506845823772
12-13	0.09184036891833054
14-15	0.061031524200769564
16-17	0.03417343316274362
18-19	0.03671739027983294
20-21	0.027584935928622895
22-23	0.040175765162511964
24-25	0.0320984082331362
26-27	0.020539229811707315
28-29	0.02334109963191168
30-31	0.0030949524373805543
32-33	0.0052403171951102565
34-35	0.007420851866901101
36-37	0.010844056835518987
38-39	0.015908524460323533
40-41	0.019777215007049223
42-43	0.0036107778436106465
44-45	0.014372771546320303
46-47	0.02362245894440082
48-49	0.0026142969452116046
50-51	0.007104322640350818
52-53	0.00220398128116494
54-55	0.004021093507657311
56-57	0.003516991406114266
58-59	9.026944609026616E-4
60-61	0.005662356163843968
62-63	0.01446655798381668
64-65	0.007467745085649292
66-67	0.03805384701415636
68-69	0.058546183607115485
70-71	0.01288391185106526
72-73	0.03158258282690611
74-75	0.04941372925590544
76-77	0.29001111134818236
78-79	0.07195764416909788
80-81	0.03295420947529067
82-83	0.027749062194241562
84-85	0.018968306983642942
86-87	0.1145601334018287
88-89	0.5125663275270932
90-91	0.1279247007450629
92-93	0.04685804883412907
94-95	0.0079132306637571
96-97	0.005580293031034636
98-99	0.009835852632432899
100-101	0.006740900195052343
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4265009.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.64350115393989
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.34429172637314	40.81133181311348
2	14.799036956880332	16.469404599747396
3	5.077615359359296	8.476088883233157
4	2.3344142501543685	5.195799280889533
5	1.2072067309597763	3.358660456360216
6	0.7003579293898389	2.338222035130467
7	0.49104713137348444	1.912650713485355
8	0.3453696475841849	1.5374061107109125
9	0.2641516488965323	1.3228490322170388
>10	1.3361083723815306	13.209232682775063
>50	0.074472249271447	2.8153563201800633
>100	0.025548619694265258	2.2441836595249005
>500	2.10765383849554E-4	0.07984234389598058
>1k	1.6861229803255868E-4	0.22897206873647827
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.3446609374095108E-5	0.0
8	0.0	0.0	0.0	2.3446609374095108E-5	0.0
9	0.0	0.0	0.0	9.378643749638043E-5	2.3446609374095108E-5
10-11	0.0	0.0	0.0	2.3446609374095108E-4	2.3446609374095108E-5
12-13	0.0	2.3446609374095108E-5	0.0	3.165292265502839E-4	9.378643749638043E-5
14-15	0.0	5.861652343523777E-5	0.0	3.516991406114266E-4	9.378643749638043E-5
16-17	0.0	7.033982812228532E-5	0.0	3.868690546725693E-4	1.1723304687047554E-4
18-19	0.0	7.033982812228532E-5	0.0	4.572088827948546E-4	1.4067965624457065E-4
20-21	0.0	7.033982812228532E-5	0.0	4.923787968559972E-4	1.6412626561866574E-4
22-23	0.0	7.033982812228532E-5	0.0	4.923787968559972E-4	1.9929617967980842E-4
24-25	0.0	7.033982812228532E-5	0.0	5.041021015430448E-4	2.3446609374095108E-4
26-27	0.0	7.033982812228532E-5	0.0	5.50995320291235E-4	2.813593124891413E-4
28-29	0.0	7.033982812228532E-5	0.0	8.792478515285665E-4	2.813593124891413E-4
30-31	0.0	8.206313280933287E-5	0.0	0.0017350490936830381	2.813593124891413E-4
32-33	0.0	9.378643749638043E-5	0.0	0.0039038604607868352	3.048059218632364E-4
34-35	0.0	1.1723304687047554E-4	0.0	0.006799516718487581	3.048059218632364E-4
36-37	0.0	1.758495703057133E-4	0.0	0.011770197905795744	3.048059218632364E-4
38-39	0.0	2.461893984279986E-4	0.0	0.019038646811765227	3.048059218632364E-4
40-41	0.0	2.6963600780209373E-4	0.0	0.027737338889554514	3.048059218632364E-4
42-43	0.0	3.6342244529847416E-4	0.0	0.036471200881404944	3.048059218632364E-4
44-45	0.0	3.751457499855217E-4	0.0	0.045193339568568316	3.048059218632364E-4
46-47	0.0	3.9859235935961685E-4	0.0	0.05406788121666332	3.165292265502839E-4
48-49	0.0	4.4548557810780704E-4	0.0	0.06383339402097393	3.516991406114266E-4
50-51	0.0	4.6893218748190216E-4	0.0	0.07388026613777368	3.516991406114266E-4
52-53	2.3446609374095108E-5	4.6893218748190216E-4	0.0	0.08370439546551953	3.751457499855217E-4
54-55	2.3446609374095108E-5	4.6893218748190216E-4	0.0	0.09452500569166442	3.9859235935961685E-4
56-57	2.3446609374095108E-5	4.8065549216894966E-4	0.0	0.10518148965219065	4.103156640466644E-4
58-59	2.3446609374095108E-5	4.923787968559972E-4	0.0	0.11588486683146507	4.220389687337119E-4
60-61	2.3446609374095108E-5	5.158254062300923E-4	0.0	0.12606069529982233	4.220389687337119E-4
62-63	2.3446609374095108E-5	5.158254062300923E-4	0.0	0.13662339282285219	4.4548557810780704E-4
64-65	2.3446609374095108E-5	5.158254062300923E-4	0.0	0.1478191487989826	4.4548557810780704E-4
66-67	2.3446609374095108E-5	5.861652343523777E-4	0.0	0.16035136150943644	4.572088827948546E-4
68-69	2.3446609374095108E-5	5.861652343523777E-4	0.0	0.1720981128058581	4.6893218748190216E-4
70-71	2.3446609374095108E-5	5.978885390394253E-4	0.0	0.18457170899287667	4.6893218748190216E-4
72-73	2.3446609374095108E-5	6.096118437264728E-4	0.0	0.19647086325022994	5.041021015430448E-4
74-75	2.3446609374095108E-5	6.096118437264728E-4	0.0	0.20800659506228475	5.275487109171399E-4
76-77	2.3446609374095108E-5	6.33058453100568E-4	0.0	0.22078499717116656	5.392720156041875E-4
78-79	2.3446609374095108E-5	6.33058453100568E-4	0.0	0.23445437043626405	5.627186249782826E-4
80-81	2.3446609374095108E-5	6.33058453100568E-4	0.0	0.24812374370136148	5.978885390394253E-4
82-83	3.516991406114266E-5	6.33058453100568E-4	0.0	0.2619220733180164	7.033982812228532E-4
84-85	4.6893218748190216E-5	6.33058453100568E-4	0.0	0.27604865546590873	7.033982812228532E-4
86-87	4.6893218748190216E-5	6.33058453100568E-4	0.0	0.29151169434812446	7.151215859099008E-4
88-89	4.6893218748190216E-5	6.33058453100568E-4	0.0	0.3074084955037609	7.268448905969483E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	6475	0.0	39.58029	1
GGTATCA	3770	0.0	37.654026	1
GTACCTG	5410	0.0	33.45976	1
TCAACGC	8065	0.0	30.980406	4
TACCTGG	5730	0.0	30.256487	2
CAACGCA	8345	0.0	29.925108	5
ATCAACG	8440	0.0	29.54693	3
TATCAAC	8660	0.0	28.959848	2
AACGCAG	9105	0.0	27.32456	6
GTACATG	12720	0.0	24.67941	1
TACATGG	13035	0.0	23.21179	2
ACCTGGG	7245	0.0	22.946962	3
ACGCAGA	10975	0.0	22.453814	7
ACATGGG	13205	0.0	21.942545	3
GTGTAGC	2710	0.0	21.26927	1
CGCAGAG	11620	0.0	21.166592	8
GAGTACA	10060	0.0	19.793144	1
AGTACAT	9505	0.0	19.3892	2
GTATAGG	1050	0.0	19.054453	1
TATCACG	350	6.366463E-11	18.999416	2
>>END_MODULE