FastQCFastQC Report
Mon 15 Aug 2016
SRR2921451_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921451_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4375325
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77200.038.757641
GGTATCA47200.031.1107141
TCAACGC98750.029.5720624
GTACCTG52250.029.3372361
CAACGCA99650.029.3049765
TATCAAC100400.029.0860652
ATCAACG103400.028.2421743
TACCTGG54150.027.9379632
AACGCAG107750.027.1805696
GTACATG136300.024.6202771
ACGCAGA126150.023.1381057
TACATGG142550.022.9620292
ACATGGG144150.021.6258663
CGCAGAG136150.021.1979718
GAGTACA105300.019.6281451
ACCTGGG74750.019.3800623
CATGGGG112800.018.6951084
GCAGAGT153500.018.6828149
AGAGTAC122250.018.04341710-11
AGTACAT101800.017.7430272
Produced by FastQC (version 0.11.5)