##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921451_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4375325
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.673178335323662	31.0	31.0	34.0	28.0	34.0
2	30.789605800711946	31.0	31.0	34.0	27.0	34.0
3	30.91966539628485	31.0	31.0	34.0	27.0	34.0
4	34.445981727071704	37.0	35.0	37.0	32.0	37.0
5	34.44654077125699	37.0	35.0	37.0	32.0	37.0
6	34.53857142040877	37.0	35.0	37.0	32.0	37.0
7	34.433683669213146	37.0	35.0	37.0	31.0	37.0
8	34.419849496894514	37.0	35.0	37.0	31.0	37.0
9	35.90784181746499	39.0	37.0	39.0	31.0	39.0
10-11	35.79304908778205	39.0	37.0	39.0	30.0	39.0
12-13	35.64651780153474	39.0	37.0	39.0	30.0	39.0
14-15	36.705149560318375	40.0	37.0	41.0	30.5	41.0
16-17	36.56827332826704	40.0	37.0	41.0	30.0	41.0
18-19	36.39652094415843	40.0	36.0	41.0	30.0	41.0
20-21	36.28842988806546	39.0	36.0	41.0	29.5	41.0
22-23	36.33418100369687	40.0	36.5	41.0	29.5	41.0
24-25	36.21487878043345	39.5	36.0	41.0	29.0	41.0
26-27	35.96752355539303	39.0	36.0	41.0	27.5	41.0
28-29	35.74773142566552	39.0	36.0	40.5	27.0	41.0
30-31	35.50620148217561	39.0	35.5	40.0	27.0	41.0
32-33	35.25665887676915	39.0	35.0	40.0	25.5	41.0
34-35	34.85492289144235	38.0	34.5	40.0	24.5	41.0
36-37	34.49202047847874	38.0	34.0	40.0	24.0	41.0
38-39	34.272946238279445	38.0	34.0	40.0	23.0	41.0
40-41	34.0185551930428	38.0	33.0	40.0	21.5	41.0
42-43	33.74738219446556	38.0	33.0	40.0	19.5	41.0
44-45	33.44508065115163	38.0	33.0	40.0	18.0	41.0
46-47	33.174746790238444	38.0	32.0	40.0	15.5	41.0
48-49	32.97601469605115	38.0	32.0	40.0	14.5	41.0
50-51	32.210272379766074	36.0	31.0	39.0	13.5	40.0
52-53	32.7140687834618	37.0	32.5	39.0	15.0	40.0
54-55	33.15982172752881	38.0	33.0	40.0	14.5	41.0
56-57	33.023619273996786	37.0	33.0	40.0	13.0	41.0
58-59	32.69447549610601	37.0	32.0	40.0	9.0	41.0
60-61	32.24445692605693	36.0	31.5	39.0	7.5	41.0
62-63	31.763876512030535	36.0	31.0	39.0	4.5	40.0
64-65	31.223323524538177	35.0	30.5	38.5	2.0	40.0
66-67	30.740948158136824	35.0	30.0	38.0	2.0	40.0
68-69	30.226581453949137	34.5	29.5	37.5	2.0	39.5
70-71	29.702829161262308	34.0	29.0	37.0	2.0	39.0
72-73	29.14610617496986	34.0	28.5	36.0	2.0	39.0
74-75	28.550715432567866	34.0	27.5	35.5	2.0	38.0
76-77	27.965080422597175	33.0	26.5	35.0	2.0	37.0
78-79	27.40401501602738	33.0	26.0	35.0	2.0	36.5
80-81	26.827399450326546	32.5	25.0	35.0	2.0	36.0
82-83	26.197112214521205	32.0	24.0	34.5	2.0	35.5
84-85	25.582382794420987	32.0	22.0	34.0	2.0	35.0
86-87	24.964400930216613	31.0	19.5	34.0	2.0	35.0
88-89	24.31178883854342	31.0	16.5	34.0	2.0	35.0
90-91	23.570880448880942	30.5	6.5	34.0	2.0	35.0
92-93	22.748429430956556	30.0	2.0	34.0	2.0	35.0
94-95	21.81812059675567	29.0	2.0	34.0	2.0	35.0
96-97	20.89974561889688	29.0	2.0	33.5	2.0	34.5
98	20.257901984424013	28.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	106713.0
3	19970.0
4	15439.0
5	13536.0
6	12120.0
7	12994.0
8	14881.0
9	17417.0
10	19540.0
11	21964.0
12	24509.0
13	28555.0
14	23652.0
15	23165.0
16	25913.0
17	29778.0
18	33941.0
19	33047.0
20	34437.0
21	38178.0
22	42053.0
23	46811.0
24	52408.0
25	59185.0
26	68007.0
27	77552.0
28	91153.0
29	107330.0
30	128160.0
31	156360.0
32	197013.0
33	256311.0
34	348090.0
35	493272.0
36	676977.0
37	713614.0
38	299751.0
39	11529.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.908401258885625	17.474765451603975	14.087554783455367	24.52927850605503
2	15.76910966714586	18.99587427974821	38.777288622291145	26.45772743081478
3	19.64415571823338	22.85951002121235	27.78374378333582	29.71259047721845
4	13.992764322774043	15.360435832815197	34.76383661483179	35.88296322957897
5	15.871407371222471	35.4982928917104	32.59411622241143	16.0361835146557
6	34.6222763884407	33.20177772736223	16.98819155600086	15.187754328196206
7	30.910749244654966	30.193703072460533	19.65675987504739	19.238787807837113
8	27.962385411911335	33.03287047376907	19.224807193917986	19.77993692040161
9	27.0343860925967	16.476719847509187	19.055133912907525	37.433760146986586
10-11	26.475407362841636	25.000891484811383	26.133991538666212	22.389709613680772
12-13	26.660087442348452	22.11595397133523	26.654407289795785	24.569551296520537
14-15	23.95282749092568	23.584174398800013	24.20755300523989	28.255445105034415
16-17	24.374467896401967	26.564028842429444	24.74147294162127	24.32003031954732
18-19	24.353346972456524	25.95556853430726	26.097166003938522	23.593918489297693
20-21	24.980737068331056	25.183928544673932	25.906227007859687	23.929107379135324
22-23	24.730275315411358	24.893587450691506	25.76264090490274	24.613496328994394
24-25	24.516161936932633	25.13108206066911	25.845433692335014	24.507322310063234
26-27	24.53216656184526	25.293399865476335	25.781983138678893	24.392450433999514
28-29	24.567894103875147	25.35375433832936	25.694518707785463	24.383832850010037
30-31	24.69015885618788	25.247936945989448	25.624004376057368	24.437899821765306
32-33	24.597007384087433	25.26455312239044	25.486641597692696	24.65179789582944
34-35	24.68168429704486	25.30525368481425	25.62404264929623	24.38901936884466
36-37	24.738976660407648	25.2489434242541	25.533318775339218	24.47876113999903
38-39	24.783463944754157	25.279145636379415	25.499655489271323	24.43773492959511
40-41	24.817606162625818	25.197839936579054	25.509474182143986	24.47507971865114
42-43	24.779025526989507	25.08373074078066	25.47827343823067	24.658970293999168
44-45	24.733191075064692	25.198817896970933	25.446577042042772	24.6214139859216
46-47	24.863958959962183	25.187346830705827	25.394040303733956	24.554653905598034
48-49	24.823789919980577	25.231369948306504	25.2695253012372	24.67531483047572
50-51	24.676664201514793	25.390375063782646	25.290344576769265	24.642616157933297
52-53	24.790071651726052	25.364711021949628	25.274302811173417	24.570914515150903
54-55	24.75156694731023	25.17585955830676	25.371576406172515	24.7009970882105
56-57	24.79310540127678	25.318566042391836	25.31197179223333	24.576356764098055
58-59	24.84481659161139	25.216252920815684	25.35299028125284	24.585940206320082
60-61	24.90399053827789	25.135571149466905	25.35061028842814	24.60982802382707
62-63	24.733687294893866	25.312261074510573	25.335387482214323	24.618664148381235
64-65	24.751357015302883	25.158056561273412	25.371391696338357	24.71919472708535
66-67	24.769824896127258	25.284539483136125	25.285642861658168	24.65999275907845
68-69	24.712348017253717	25.265996372886395	25.32231244690929	24.699343162950594
70-71	24.677821149731223	25.091891575347304	25.413390087502393	24.816897187419084
72-73	24.77396767002638	25.124607235353636	25.286804726642465	24.814620367977522
74-75	24.633392105991426	25.300716784619464	25.26134625849723	24.80454485089188
76-77	24.810270532385914	25.125763073619673	25.249643432125364	24.81432296186905
78-79	24.754282612905563	25.05481081015308	25.363868130798412	24.82703844614295
80-81	24.725342842200526	25.221176667216127	25.23617967240875	24.817300818174598
82-83	24.848584979097197	25.10306738266943	25.21347129842629	24.83487633980709
84-85	24.809357742673555	25.166556961981733	25.161254409468388	24.86283088587633
86-87	24.764247204825793	25.099798955892876	25.329893224873274	24.806060614408054
88-89	24.76608421450472	24.99300244564238	25.27070030421853	24.97021303563437
90-91	24.779633662127523	25.045737709246147	25.250739528653078	24.923889099973255
92-93	24.74503517338067	25.121746063411948	25.31367704218074	24.819541721026642
94-95	24.88224947751255	24.974888689452467	25.240523307084406	24.902338525950572
96-97	24.89753976045012	24.964541823888688	25.217179893825204	24.920738521835993
98	24.87622938438151	25.056025630606864	25.146750192032126	24.9209947929795
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	534.0
1	402.5
2	436.0
3	576.0
4	473.5
5	469.0
6	731.0
7	1453.5
8	2128.5
9	2095.0
10	1707.5
11	1537.0
12	1745.0
13	2084.0
14	2451.0
15	2725.0
16	2893.0
17	3040.5
18	3080.0
19	2956.0
20	2742.0
21	2505.5
22	2388.0
23	2395.5
24	2502.0
25	2815.5
26	3433.0
27	4363.5
28	5695.5
29	7758.5
30	10825.0
31	15166.5
32	20939.0
33	28081.5
34	36778.5
35	47484.5
36	59280.0
37	72224.0
38	85913.0
39	98613.5
40	111960.0
41	126085.5
42	139581.5
43	153367.0
44	166759.0
45	178848.0
46	191520.5
47	203391.5
48	208658.5
49	207859.5
50	206821.0
51	205303.0
52	204409.0
53	203396.5
54	198255.0
55	190568.0
56	178121.0
57	163942.5
58	150019.0
59	134129.5
60	116371.0
61	96638.5
62	77042.0
63	58091.0
64	42237.5
65	31191.5
66	23792.5
67	18246.5
68	13745.0
69	10293.5
70	7566.0
71	5568.0
72	4061.5
73	2885.0
74	2107.0
75	1546.0
76	1035.5
77	600.0
78	355.5
79	207.5
80	128.0
81	74.0
82	48.5
83	34.5
84	23.5
85	21.5
86	19.0
87	16.5
88	10.0
89	5.0
90	3.5
91	6.0
92	6.5
93	5.5
94	4.5
95	2.0
96	1.5
97	2.5
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.47265060309805557
2	0.0463508425088422
3	0.054875923502825504
4	0.051927571094718676
5	0.06230394313565278
6	0.06344671538685698
7	0.04175689805900133
8	0.019472839160519506
9	0.011519144292138298
10-11	0.013736122459474441
12-13	0.010113534423157138
14-15	0.014078954134835698
16-17	0.013473284841697474
18-19	0.054064555204470524
20-21	0.081971053578877
22-23	0.09707850273979647
24-25	0.06784638855399314
26-27	0.08235959614428642
28-29	0.08416517630118905
30-31	0.05574443041374069
32-33	0.03182620719603686
34-35	0.39470210784341736
36-37	0.6216338214875466
38-39	0.5703004919634541
40-41	0.564803757435162
42-43	0.5388856827778508
44-45	0.5236753841143229
46-47	0.5143046516544485
48-49	0.5044082439590202
50-51	0.5177215406855491
52-53	0.5979555804425957
54-55	0.5692719969373703
56-57	0.5267837246375984
58-59	0.5048539251369898
60-61	0.5171958654499952
62-63	0.5292749681452236
64-65	0.5475136132744425
66-67	0.5725288978533023
68-69	0.528052201836435
70-71	0.47705027626519175
72-73	0.4603200905075623
74-75	0.44052727511670564
76-77	0.4551204767645832
78-79	0.449349476896002
80-81	0.44638969676538315
82-83	0.4664110666064807
84-85	0.4327107129184689
86-87	0.4357847702742082
88-89	0.462411363727266
90-91	0.4459097324198774
92-93	0.4418414632055904
94-95	0.45066366498488686
96-97	0.4454297680743716
98	0.44072154639941036
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4375325.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.94745472661947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.34670690192216	40.85162311566614
2	13.881800185491432	15.255391744325367
3	4.856770285744447	8.00601496180601
4	2.2743397231037377	4.998767158727794
5	1.1723576608497492	3.2209034746473555
6	0.7685665969080545	2.5338466972798392
7	0.5161324556212948	1.9852115316733019
8	0.36047277448489085	1.584564916494996
9	0.27132228543133313	1.341762209555499
>10	1.4376325580181937	14.184606783110763
>50	0.08461134078364016	3.1551448603655086
>100	0.02870265073246793	2.494298696294922
>500	4.1755782290467614E-4	0.15030443243972438
>1k	1.6702308564617586E-4	0.2375594176126949
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1427722512041962E-4
2	0.0	0.0	0.0	2.2855445024083926E-5	1.1427722512041962E-4
3	0.0	0.0	0.0	2.2855445024083926E-5	1.1427722512041962E-4
4	0.0	0.0	0.0	2.2855445024083926E-5	1.1427722512041962E-4
5	0.0	0.0	0.0	2.2855445024083926E-5	1.1427722512041962E-4
6	0.0	0.0	0.0	2.2855445024083926E-5	1.1427722512041962E-4
7	0.0	0.0	0.0	2.2855445024083926E-5	1.1427722512041962E-4
8	0.0	2.2855445024083926E-5	0.0	2.2855445024083926E-5	1.1427722512041962E-4
9	0.0	2.2855445024083926E-5	0.0	2.2855445024083926E-5	1.1427722512041962E-4
10-11	0.0	2.2855445024083926E-5	0.0	5.713861256020981E-5	1.1427722512041962E-4
12-13	0.0	2.2855445024083926E-5	0.0	2.2855445024083925E-4	1.9427128270471336E-4
14-15	0.0	2.2855445024083926E-5	0.0	2.2855445024083925E-4	2.2855445024083925E-4
16-17	0.0	2.2855445024083926E-5	0.0	2.7426534028900714E-4	2.6283761777696514E-4
18-19	0.0	2.2855445024083926E-5	0.0	3.656871203853428E-4	2.9712078531309103E-4
20-21	0.0	2.2855445024083926E-5	0.0	3.656871203853428E-4	3.542593978733008E-4
22-23	0.0	2.2855445024083926E-5	0.0	3.7711484289738476E-4	3.656871203853428E-4
24-25	0.0	2.2855445024083926E-5	0.0	4.1139801043351066E-4	3.7711484289738476E-4
26-27	0.0	2.2855445024083926E-5	0.0	5.028197905298464E-4	3.885425654094267E-4
28-29	0.0	2.2855445024083926E-5	0.0	7.885128533308954E-4	3.885425654094267E-4
30-31	0.0	2.2855445024083926E-5	0.0	0.0017712969893665044	3.885425654094267E-4
32-33	0.0	2.2855445024083926E-5	0.0	0.004182546439407358	3.885425654094267E-4
34-35	0.0	2.2855445024083926E-5	0.0	0.007130898847514185	3.885425654094267E-4
36-37	0.0	2.2855445024083926E-5	0.0	0.011976253192619976	3.885425654094267E-4
38-39	0.0	4.571089004816785E-5	0.0	0.01900430253752578	4.342534554575946E-4
40-41	0.0	4.571089004816785E-5	0.0	0.027060846908515367	4.342534554575946E-4
42-43	0.0	4.571089004816785E-5	0.0	0.03602018135795627	4.342534554575946E-4
44-45	0.0	4.571089004816785E-5	0.0	0.04524235342517413	4.4568117796963655E-4
46-47	0.0	4.571089004816785E-5	0.0	0.055355887848331266	4.571089004816785E-4
48-49	0.0	4.571089004816785E-5	0.0	0.06522944009873552	4.7996434550576244E-4
50-51	0.0	4.571089004816785E-5	0.0	0.07516013096169999	5.256752355539303E-4
52-53	0.0	6.856633507225177E-5	0.0	0.08562792478273043	5.828138481141401E-4
54-55	0.0	7.999405758429373E-5	0.0	0.09683852056704359	6.17097015650266E-4
56-57	0.0	1.1427722512041962E-4	0.0	0.10840337574923006	6.513801831863919E-4
58-59	0.0	1.1427722512041962E-4	0.0	0.12068817744967517	6.856633507225177E-4
60-61	0.0	1.2570494763246157E-4	0.0	0.1338186306160114	6.856633507225177E-4
62-63	0.0	1.3713267014450354E-4	0.0	0.14611486003896854	7.313742407706856E-4
64-65	0.0	1.4856039265654552E-4	0.0	0.16007953694868382	7.313742407706856E-4
66-67	0.0	2.171267277287973E-4	0.0	0.17510699205201902	7.313742407706856E-4
68-69	0.0	2.2855445024083925E-4	0.0	0.19027157982549867	7.542296857947695E-4
70-71	0.0	2.2855445024083925E-4	0.0	0.2058018547193637	7.656574083068114E-4
72-73	0.0	2.2855445024083925E-4	0.0	0.22104643655042766	8.113682983549793E-4
74-75	0.0	2.2855445024083925E-4	0.0	0.2366224223343409	8.456514658911052E-4
76-77	0.0	2.2855445024083925E-4	0.0	0.2536268734322593	8.456514658911052E-4
78-79	0.0	2.2855445024083925E-4	0.0	0.2708370235353945	8.570791884031472E-4
80-81	0.0	2.2855445024083925E-4	0.0	0.28936136172741456	9.14217800963357E-4
82-83	0.0	2.2855445024083925E-4	0.0	0.3086742127727654	0.0010284950260837766
84-85	0.0	2.2855445024083925E-4	0.0	0.32779279253541166	0.0010284950260837766
86	0.0	2.2855445024083925E-4	0.0	0.3423745664607772	0.0010284950260837766
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7720	0.0	38.75764	1
GGTATCA	4720	0.0	31.110714	1
TCAACGC	9875	0.0	29.572062	4
GTACCTG	5225	0.0	29.337236	1
CAACGCA	9965	0.0	29.304976	5
TATCAAC	10040	0.0	29.086065	2
ATCAACG	10340	0.0	28.242174	3
TACCTGG	5415	0.0	27.937963	2
AACGCAG	10775	0.0	27.180569	6
GTACATG	13630	0.0	24.620277	1
ACGCAGA	12615	0.0	23.138105	7
TACATGG	14255	0.0	22.962029	2
ACATGGG	14415	0.0	21.625866	3
CGCAGAG	13615	0.0	21.197971	8
GAGTACA	10530	0.0	19.628145	1
ACCTGGG	7475	0.0	19.380062	3
CATGGGG	11280	0.0	18.695108	4
GCAGAGT	15350	0.0	18.682814	9
AGAGTAC	12225	0.0	18.043417	10-11
AGTACAT	10180	0.0	17.743027	2
>>END_MODULE