FastQCFastQC Report
Mon 15 Aug 2016
SRR2921451_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921451_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4375325
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45020.10289521349842583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78950.041.931551
GGTATCA44950.034.9697461
TCAACGC99600.032.4744074
TATCAAC102150.031.8489932
CAACGCA102250.031.7126055
ATCAACG105600.030.5389673
AACGCAG111200.029.2058966
GTACCTG54950.026.7854541
TACCTGG57250.025.551642
ACGCAGA131450.024.7081137
GTACATG139050.024.6986081
TACATGG142200.023.5468642
CGCAGAG141400.022.969468
ACATGGG145850.022.1111763
TATCACG4150.020.6000332
GCAGAGT161600.019.6274689
ACCTGGG75150.018.9601063
GAGTACA111000.018.7957361
AGAGTAC129550.018.47214510-11
AGTACAT103450.018.18062
Produced by FastQC (version 0.11.5)