##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921451_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4375325
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03050287692914	33.0	31.0	34.0	30.0	34.0
2	32.149379303251756	34.0	31.0	34.0	30.0	34.0
3	32.27176449749447	34.0	31.0	34.0	30.0	34.0
4	35.813663442144296	37.0	35.0	37.0	35.0	37.0
5	35.62890459565861	37.0	35.0	37.0	35.0	37.0
6	35.66537685771914	37.0	35.0	37.0	35.0	37.0
7	35.6013237873758	37.0	35.0	37.0	33.0	37.0
8	35.55822458903053	37.0	35.0	37.0	33.0	37.0
9	37.19710741487775	39.0	37.0	39.0	34.0	39.0
10-11	37.11448372406621	39.0	37.0	39.0	34.0	39.0
12-13	37.00443087084959	39.0	37.0	39.0	33.0	39.0
14-15	38.32226440321576	40.0	38.0	41.0	34.0	41.0
16-17	38.22817310714061	40.0	38.0	41.0	33.0	41.0
18-19	38.12650454537663	40.0	38.0	41.0	33.5	41.0
20-21	38.01937981749927	40.0	38.0	41.0	33.0	41.0
22-23	37.990086907829706	40.0	38.0	41.0	33.0	41.0
24-25	38.04798089284796	40.0	38.0	41.0	33.0	41.0
26-27	37.94899796015153	40.0	38.0	41.0	33.0	41.0
28-29	37.82030443452772	40.0	38.0	41.0	33.0	41.0
30-31	37.68732368452629	40.0	38.0	41.0	33.0	41.0
32-33	37.53696765383143	40.0	37.5	41.0	32.0	41.0
34-35	37.40460491506345	40.0	37.0	41.0	32.0	41.0
36-37	37.31866467062447	40.0	37.0	41.0	32.0	41.0
38-39	37.29785753058344	40.0	37.0	41.0	32.0	41.0
40-41	37.143604646511974	40.0	37.0	41.0	31.0	41.0
42-43	36.922000308548505	40.0	36.5	41.0	31.0	41.0
44-45	36.733155936987544	39.5	36.0	41.0	30.5	41.0
46-47	36.4777467959523	39.0	36.0	41.0	30.0	41.0
48-49	36.1621585825053	39.0	35.0	40.0	30.0	41.0
50-51	35.896123945078365	39.0	35.0	40.0	29.0	41.0
52-53	35.59478061629707	38.0	35.0	40.0	28.0	41.0
54-55	35.27768851456749	38.0	34.5	40.0	28.0	41.0
56-57	34.912279430670864	38.0	34.0	40.0	27.0	41.0
58-59	34.53021421265849	37.5	34.0	40.0	26.0	41.0
60-61	34.1132335312234	37.0	33.0	39.5	26.0	41.0
62-63	33.67805145903448	36.0	33.0	39.0	25.5	40.0
64-65	33.17066800751944	36.0	32.0	39.0	23.5	40.0
66-67	32.64977813076743	35.0	32.0	38.0	22.5	40.0
68-69	32.15222629176118	35.0	31.0	37.5	22.0	40.0
70-71	31.60183209247313	35.0	31.0	37.0	20.0	39.0
72-73	31.053582076760012	34.0	30.0	36.0	18.5	39.0
74-75	30.533839543348208	34.0	30.0	36.0	17.5	38.5
76-77	29.221665133447228	32.5	28.0	34.5	16.0	36.0
78-79	30.167701713586993	34.0	30.0	35.0	17.5	37.0
80-81	30.20972327769937	34.0	30.0	35.0	17.0	36.5
82-83	29.88269042871101	34.0	30.0	35.0	12.5	36.0
84-85	29.40250312833904	34.0	29.0	35.0	7.0	36.0
86-87	28.892126984852553	34.0	29.0	35.0	3.5	35.0
88-89	28.36300640523847	33.0	28.5	35.0	2.0	35.0
90-91	27.904448126710587	33.0	27.0	35.0	2.0	35.0
92-93	27.480923931365098	33.0	27.0	34.5	2.0	35.0
94-95	26.997490472136356	32.5	25.5	34.0	2.0	35.0
96-97	26.24281190540131	32.0	25.0	34.0	2.0	35.0
98-99	25.238550964785475	32.0	21.5	34.0	2.0	35.0
100-101	21.583295183786348	28.0	2.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	22387.0
3	12442.0
4	7449.0
5	5663.0
6	4956.0
7	5342.0
8	6014.0
9	7335.0
10	8455.0
11	10328.0
12	12344.0
13	14350.0
14	16021.0
15	18190.0
16	20521.0
17	24209.0
18	25039.0
19	22524.0
20	22651.0
21	24404.0
22	25917.0
23	28872.0
24	33414.0
25	38715.0
26	46986.0
27	56708.0
28	69804.0
29	85123.0
30	105472.0
31	132724.0
32	170933.0
33	231849.0
34	331801.0
35	506110.0
36	796710.0
37	991275.0
38	421030.0
39	11258.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.30702342854759	17.920296258118004	14.394665079482264	24.37801523385215
2	15.902201550741946	19.278476456034692	38.48148423259987	26.337837760623493
3	19.665000935987806	23.034625606020676	28.01296074542572	29.287412712565803
4	14.209830151261244	15.69022089117533	34.82928225918581	35.27066669837762
5	15.882375393372216	35.51559444144402	32.69629642157845	15.905733743605307
6	34.660928877764604	33.30705495542968	16.872548266221695	15.159467900584026
7	30.95347777595809	30.189513469102447	19.71709532536188	19.139913429577586
8	27.973456452149108	33.11038296776048	19.239633471520918	19.6765271085695
9	26.99925786493874	16.41502553548568	19.145002758985463	37.44071384059012
10-11	26.470352725422515	25.05273402467907	26.194214036539076	22.282699213359347
12-13	26.62729115662787	22.142129782468096	26.750478324023085	24.48010073688095
14-15	23.92937301073706	23.611811371817108	24.285723597414176	28.173092020031653
16-17	24.32043158016277	26.60488442838378	24.872344779414203	24.20233921203925
18-19	24.331529802052085	26.017197244480712	26.186121821646243	23.46515113182096
20-21	24.96000259714488	25.164904982448693	26.097465177454037	23.777627242952388
22-23	24.717711984537484	24.841974160007016	25.99269975439099	24.44761410106451
24-25	24.471520718024884	25.130687991337464	26.01942806398865	24.37836322664901
26-27	24.489814113442613	25.286330359299292	25.926240892527975	24.29761463473012
28-29	24.50289370234673	25.340719148844236	25.92084271543826	24.235544433370777
30-31	24.672480424593502	25.219575852193156	25.782796269836112	24.325147453377237
32-33	24.499585323432292	25.212346744808944	25.727203959958366	24.56086397180039
34-35	24.65270210238758	25.24085679062491	25.84858882532378	24.25785228166373
36-37	24.704709617170813	25.250803534764373	25.713295321697917	24.3311915263669
38-39	24.673046953800544	25.262965399124166	25.74709306810967	24.316894578965627
40-41	24.779279959410122	25.13766463492483	25.699353033079632	24.383702372585418
42-43	24.705250442555748	25.140949292082222	25.679890198175837	24.473910067186193
44-45	24.67829576045434	25.139180294385937	25.642708420126358	24.539815525033365
46-47	24.77024527250907	25.174675776040534	25.64408995478119	24.4109889966692
48-49	24.74821324514497	25.288899509403095	25.452709893911805	24.510177351540126
50-51	24.674869314584424	25.370376296282753	25.42910759061122	24.525646798521603
52-53	24.777428335032294	25.38008374436028	25.341605679257274	24.50088224135015
54-55	24.771357778292558	25.18342633456008	25.462550011468256	24.582665875679112
56-57	24.784744375446127	25.33032604390207	25.414266080659694	24.470663499992114
58-59	24.827278993907566	25.273092092271458	25.388947958340342	24.51068095548063
60-61	24.93556950659116	25.164971891265118	25.457996209655015	24.441462392488702
62-63	24.71714103659425	25.328433907758598	25.43255622576599	24.521868829881164
64-65	24.763572583436886	25.18296988881177	25.433520264203047	24.619937263548298
66-67	24.770098168502187	25.32835156965683	25.33098101165609	24.57056925018489
68-69	24.75416014684297	25.259996313374174	25.379091251307585	24.60675228847527
70-71	24.742358061633364	25.134382747987903	25.44625381552598	24.677005374852747
72-73	24.84226951732889	25.172151457387248	25.3197335291949	24.665845496088963
74-75	24.69058899342463	25.328882827431876	25.22658430646702	24.753943872676473
76-77	24.80053039063123	25.152863274942067	25.35312379357681	24.69348254084989
78-79	24.73533980540332	25.143933586584932	25.455307939479905	24.665418668531842
80-81	24.701200183502202	25.29098622601751	25.34425810844349	24.6635554820368
82-83	24.79487428626951	25.237121415629773	25.283943278787845	24.684061019312875
84-85	24.740790135880363	25.31638914095523	25.259569913538986	24.683250809625413
86-87	24.71605786059413	25.228377360873814	25.372343511037272	24.68322126749478
88-89	24.739642003086992	25.09639814504728	25.3394743841094	24.824485467756325
90-91	24.704882900647497	25.2521025519905	25.286466128933334	24.75654841842867
92-93	24.774926187314097	25.307141263029266	25.32319346423881	24.59473908541783
94-95	24.797224856836536	25.17559217107547	25.260701102393234	24.76648186969476
96-97	24.7407381841936	25.19144644002419	25.340528269663377	24.727287106118833
98-99	24.74203628532251	25.18773413194293	25.329370457220296	24.740859125514262
100-101	25.263149473746765	24.66297107507201	25.389468535376597	24.684410915804627
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	218.0
1	178.5
2	279.0
3	423.0
4	329.5
5	280.0
6	531.5
7	1150.0
8	1755.0
9	1776.5
10	1304.5
11	941.0
12	893.5
13	959.0
14	1032.5
15	1120.5
16	1209.0
17	1301.5
18	1393.0
19	1435.0
20	1512.0
21	1583.0
22	1734.0
23	1971.0
24	2198.0
25	2546.0
26	3054.5
27	3915.0
28	5203.0
29	6864.5
30	9498.5
31	13305.0
32	18280.5
33	24302.5
34	31387.5
35	40056.0
36	50549.5
37	62229.0
38	74936.0
39	87735.5
40	100840.5
41	113918.0
42	126680.5
43	140871.5
44	155409.0
45	169374.5
46	180676.0
47	190825.0
48	201959.5
49	207354.0
50	204900.5
51	202160.5
52	201780.0
53	199531.5
54	194955.0
55	188109.0
56	176372.0
57	161014.0
58	146627.0
59	132656.5
60	115389.5
61	96658.5
62	78194.0
63	59176.0
64	42788.5
65	31722.0
66	24070.0
67	18478.0
68	14064.0
69	10436.0
70	7832.0
71	5897.5
72	4265.0
73	3045.0
74	2206.0
75	1521.5
76	1009.0
77	595.5
78	314.0
79	172.5
80	102.5
81	54.0
82	26.0
83	14.0
84	7.5
85	2.0
86	2.0
87	1.5
88	1.0
89	0.0
90	0.0
91	0.0
92	1.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3136452720655037
2	0.0
3	0.006125259266454492
4	0.056978624445041225
5	3.656871203853428E-4
6	0.06648648957506013
7	0.07754852496671676
8	0.1073291698330981
9	0.09491866318502054
10-11	0.09903264328935564
12-13	0.09905549873437973
14-15	0.0673207133184392
16-17	0.0372543753892568
18-19	0.04057984264026101
20-21	0.02950637952609235
22-23	0.04366532771851234
24-25	0.035403084342306
26-27	0.02202122128070486
28-29	0.024786730128619015
30-31	0.002936924685594784
32-33	0.005222469188003177
34-35	0.008467942381423094
36-37	0.009976401753012632
38-39	0.014913177878214763
40-41	0.019095724317622118
42-43	0.0038854256540942675
44-45	0.015850251124202203
46-47	0.026272334055184473
48-49	0.0033026118059801273
50-51	0.007199465182586436
52-53	0.002399821727528812
54-55	0.0046510830624010785
56-57	0.004068269214286938
58-59	0.0013941821464691195
60-61	0.005976698873797947
62-63	0.015141732328455601
64-65	0.00814796615108592
66-67	0.040534131750212844
68-69	0.06314959460154389
70-71	0.013724694736962396
72-73	0.03399747447332484
74-75	0.053458885911332306
76-77	0.3029946346842806
78-79	0.0744287567209293
80-81	0.03509453583448086
82-83	0.029712078531309104
84-85	0.02115271436978967
86-87	0.11885974184774845
88-89	0.5300749087210664
90-91	0.13423002862644487
92-93	0.04783644643540766
94-95	0.007850845365772829
96-97	0.0048224989000817085
98-99	0.008867912669344563
100-101	0.0066852176695445485
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4375325.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.70048863195918
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.3933388525463	36.910705037436806
2	15.335414033460083	15.856967978065809
3	5.424069827377536	8.412811813478552
4	2.4983360105949326	5.1666077005831035
5	1.4033961629452494	3.6278133684243006
6	0.8688025073592525	2.69505084930868
7	0.5846911239278139	2.116017176408617
8	0.4230925021411269	1.7499271277771602
9	0.312178440490478	1.4525800122348635
>10	1.6208659371456917	15.150216297979014
>50	0.10038600170596246	3.5366933670977985
>100	0.03463211874515689	2.8513770537445806
>500	5.752367629719789E-4	0.19143421492970167
>1k	2.2124479752094482E-4	0.2817980025311527
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4502	0.10289521349842583	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.856633507225177E-5
2	0.0	0.0	0.0	0.0	6.856633507225177E-5
3	0.0	0.0	0.0	0.0	6.856633507225177E-5
4	0.0	0.0	0.0	6.856633507225177E-5	6.856633507225177E-5
5	0.0	0.0	0.0	6.856633507225177E-5	6.856633507225177E-5
6	0.0	0.0	0.0	6.856633507225177E-5	6.856633507225177E-5
7	0.0	2.2855445024083926E-5	0.0	6.856633507225177E-5	6.856633507225177E-5
8	0.0	2.2855445024083926E-5	0.0	6.856633507225177E-5	6.856633507225177E-5
9	0.0	2.2855445024083926E-5	0.0	6.856633507225177E-5	6.856633507225177E-5
10-11	0.0	2.2855445024083926E-5	0.0	1.599881151685875E-4	6.856633507225177E-5
12-13	0.0	3.428316753612589E-5	0.0	2.2855445024083925E-4	1.5998811516858746E-4
14-15	0.0	7.999405758429373E-5	0.0	2.2855445024083925E-4	2.171267277287973E-4
16-17	0.0	1.1427722512041962E-4	0.0	2.7426534028900714E-4	2.514098952649232E-4
18-19	0.0	1.1427722512041962E-4	0.0	3.656871203853428E-4	2.742653402890071E-4
20-21	0.0	1.2570494763246157E-4	0.0	3.656871203853428E-4	3.19976230337175E-4
22-23	0.0	1.3713267014450354E-4	0.0	3.885425654094267E-4	3.428316753612589E-4
24-25	0.0	1.3713267014450354E-4	0.0	4.1139801043351066E-4	3.885425654094267E-4
26-27	0.0	1.3713267014450354E-4	0.0	4.7996434550576244E-4	3.885425654094267E-4
28-29	0.0	1.3713267014450354E-4	0.0	7.542296857947696E-4	3.885425654094267E-4
30-31	0.0	1.3713267014450354E-4	0.0	0.0017255860993183364	3.999702879214687E-4
32-33	0.0	1.3713267014450354E-4	0.0	0.004239685051967567	4.1139801043351066E-4
34-35	0.0	1.3713267014450354E-4	0.0	0.007199465182586437	4.342534554575946E-4
36-37	0.0	1.4856039265654552E-4	0.0	0.01211338586276448	4.571089004816785E-4
38-39	0.0	1.599881151685875E-4	0.0	0.019301423322838875	4.571089004816785E-4
40-41	0.0	1.599881151685875E-4	0.0	0.027277973636244165	4.571089004816785E-4
42-43	0.0	1.599881151685875E-4	0.0	0.03631730214326935	5.142475130418883E-4
44-45	0.0	1.599881151685875E-4	0.0	0.04553947421048722	5.599584030900562E-4
46-47	0.0	1.599881151685875E-4	0.0	0.05571014724620457	6.056692931382241E-4
48-49	0.0	1.7141583768062946E-4	0.0	0.06542371138144024	6.17097015650266E-4
50-51	0.0	1.828435601926714E-4	0.0	0.07525155274179632	6.628079056984339E-4
52-53	0.0	1.828435601926714E-4	0.0	0.08555935844765816	6.856633507225177E-4
54-55	0.0	1.828435601926714E-4	0.0	0.09643855027912213	7.313742407706856E-4
56-57	0.0	1.828435601926714E-4	0.0	0.10774056784353161	7.656574083068114E-4
58-59	0.0	1.9427128270471336E-4	0.0	0.11987680915132018	7.770851308188534E-4
60-61	0.0	2.2855445024083925E-4	0.0	0.13288155737002394	7.770851308188534E-4
62-63	0.0	2.2855445024083925E-4	0.0	0.1450177986778125	7.999405758429374E-4
64-65	0.0	2.2855445024083925E-4	0.0	0.1588567706398953	7.999405758429374E-4
66-67	0.0	2.2855445024083925E-4	0.0	0.17355282179038128	8.113682983549794E-4
68-69	0.0	2.2855445024083925E-4	0.0	0.18848885511362012	8.227960208670213E-4
70-71	0.0	2.3998217275288122E-4	0.0	0.20380200327975637	9.02790078451315E-4
72-73	0.0	2.514098952649232E-4	0.0	0.21874946432550724	0.0010513504711078606
74-75	0.0	2.514098952649232E-4	0.0	0.23414260654922778	0.0010856336386439866
76-77	0.0	2.742653402890071E-4	0.0	0.2510099249770017	0.0011199168061801123
78-79	0.0	2.742653402890071E-4	0.0	0.26783153251472747	0.0011199168061801123
80-81	0.0	2.8569306280104903E-4	0.0	0.28594447269631396	0.0012113385862764481
82-83	2.2855445024083926E-5	2.9712078531309103E-4	0.0	0.3046859376160628	0.0013713267014450355
84-85	2.2855445024083926E-5	3.0854850782513303E-4	0.0	0.32359881837349225	0.0013941821464691195
86-87	2.2855445024083926E-5	3.19976230337175E-4	0.0	0.3432545010942044	0.0014741762040534133
88-89	2.2855445024083926E-5	3.19976230337175E-4	0.0	0.3643500768514339	0.0015655979841497488
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7895	0.0	41.93155	1
GGTATCA	4495	0.0	34.969746	1
TCAACGC	9960	0.0	32.474407	4
TATCAAC	10215	0.0	31.848993	2
CAACGCA	10225	0.0	31.712605	5
ATCAACG	10560	0.0	30.538967	3
AACGCAG	11120	0.0	29.205896	6
GTACCTG	5495	0.0	26.785454	1
TACCTGG	5725	0.0	25.55164	2
ACGCAGA	13145	0.0	24.708113	7
GTACATG	13905	0.0	24.698608	1
TACATGG	14220	0.0	23.546864	2
CGCAGAG	14140	0.0	22.96946	8
ACATGGG	14585	0.0	22.111176	3
TATCACG	415	0.0	20.600033	2
GCAGAGT	16160	0.0	19.627468	9
ACCTGGG	7515	0.0	18.960106	3
GAGTACA	11100	0.0	18.795736	1
AGAGTAC	12955	0.0	18.472145	10-11
AGTACAT	10345	0.0	18.1806	2
>>END_MODULE