FastQCFastQC Report
Mon 15 Aug 2016
SRR2921450_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921450_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4625323
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG154700.029.4577081
GTACCTG43050.029.1906381
TACATGG158350.028.1673132
ACATGGG161900.026.8135173
GTATCAA104500.025.4604241
GAGTACA111050.025.4094921
TACCTGG48200.025.2028392
AGTACAT106650.024.3709742
CATGGGG128550.022.5013184
TCAACGC121900.021.3973124
CAACGCA124850.021.0018775
TATCAAC128550.020.361732
ATCAACG128150.020.3179723
AACGCAG130700.020.1966846
ACCTGGG66050.018.2529723
ACGCAGA150950.017.4529697
GTGTAGC24300.017.427521
ATGGGGG63800.017.1004265
GGTATCA80000.016.7439171
AGAGTAC116150.016.50576410-11
Produced by FastQC (version 0.11.5)