##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921450_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4625323
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.184683102131462	31.0	31.0	34.0	27.0	34.0
2	30.269526474151103	31.0	31.0	34.0	26.0	34.0
3	30.40771012100128	31.0	31.0	34.0	26.0	34.0
4	33.89904013189998	37.0	35.0	37.0	30.0	37.0
5	33.875862939734155	37.0	35.0	37.0	30.0	37.0
6	33.97176110727835	37.0	35.0	37.0	30.0	37.0
7	33.84604534645472	37.0	35.0	37.0	30.0	37.0
8	33.82186044088164	37.0	35.0	37.0	30.0	37.0
9	35.252477070250016	39.0	35.0	39.0	30.0	39.0
10-11	35.12226724490376	39.0	35.0	39.0	29.0	39.0
12-13	34.94730346399592	39.0	35.0	39.0	28.0	39.0
14-15	35.94528479416464	40.0	36.0	41.0	27.0	41.0
16-17	35.79034253391601	39.5	36.0	41.0	27.0	41.0
18-19	35.58929473682163	39.0	36.0	41.0	27.0	41.0
20-21	35.46325251231103	39.0	36.0	40.0	27.0	41.0
22-23	35.50106630391002	39.0	36.0	41.0	26.5	41.0
24-25	35.356173612091524	39.0	36.0	41.0	25.5	41.0
26-27	35.071836172306234	39.0	35.0	40.5	24.5	41.0
28-29	34.82442804534948	39.0	35.0	40.0	24.0	41.0
30-31	34.557914333766526	38.5	34.0	40.0	23.0	41.0
32-33	34.28262091101529	38.0	34.0	40.0	21.0	41.0
34-35	33.85325662661829	38.0	33.5	40.0	17.5	41.0
36-37	33.45444037097517	38.0	33.0	40.0	15.0	41.0
38-39	33.20664308200746	38.0	33.0	40.0	12.5	41.0
40-41	32.921592286635985	38.0	32.0	40.0	9.0	41.0
42-43	32.61059984351363	38.0	31.5	40.0	8.5	41.0
44-45	32.279402973586926	37.0	31.0	40.0	8.0	41.0
46-47	31.97442935336624	37.0	31.0	40.0	6.5	41.0
48-49	31.74939739343609	37.0	30.0	40.0	2.0	41.0
50-51	31.009126497760263	36.0	29.5	39.0	2.0	40.0
52-53	31.529958772608964	36.5	30.5	39.0	2.0	40.0
54-55	31.942604116512513	37.0	31.0	40.0	2.0	40.5
56-57	31.77034436297746	36.5	31.0	40.0	2.0	41.0
58-59	31.419512107586865	36.0	31.0	39.0	2.0	41.0
60-61	30.94155575729522	35.5	30.0	39.0	2.0	40.0
62-63	30.437113797241835	35.0	29.0	39.0	2.0	40.0
64-65	29.878365143364043	35.0	28.5	38.0	2.0	40.0
66-67	29.380323925485854	34.5	28.0	37.5	2.0	40.0
68-69	28.844025703718422	34.0	27.5	37.0	2.0	39.0
70-71	28.312415586976304	34.0	26.0	36.0	2.0	39.0
72-73	27.747905930029102	33.5	26.0	36.0	2.0	38.5
74-75	27.145802358019104	33.0	25.0	35.0	2.0	37.5
76-77	26.55911846588876	33.0	24.0	35.0	2.0	37.0
78-79	25.990932849446406	32.0	22.0	35.0	2.0	36.0
80-81	25.405299478544528	32.0	19.5	35.0	2.0	36.0
82-83	24.76661111018625	31.0	17.5	34.0	2.0	35.0
84-85	24.13581948763362	31.0	9.5	34.0	2.0	35.0
86-87	23.503632719271714	30.5	4.5	34.0	2.0	35.0
88-89	22.845206594220556	30.0	2.0	34.0	2.0	35.0
90-91	22.090710421737036	29.5	2.0	34.0	2.0	35.0
92-93	21.26044354091595	29.0	2.0	34.0	2.0	35.0
94-95	20.336702215175027	28.0	2.0	33.5	2.0	34.5
96-97	19.43626585646019	27.0	2.0	33.0	2.0	34.0
98	18.816691072169448	25.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	166729.0
3	28324.0
4	21434.0
5	18403.0
6	17223.0
7	17986.0
8	20450.0
9	23555.0
10	26190.0
11	28602.0
12	31105.0
13	34659.0
14	30354.0
15	29813.0
16	33424.0
17	37708.0
18	42032.0
19	41866.0
20	43514.0
21	47529.0
22	51913.0
23	56508.0
24	62959.0
25	70257.0
26	79523.0
27	90185.0
28	103809.0
29	120738.0
30	142919.0
31	171475.0
32	211747.0
33	270804.0
34	360411.0
35	494167.0
36	655866.0
37	658854.0
38	270159.0
39	12129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.92036973722452	16.140119408250143	11.386749185763978	25.55276166876136
2	16.97665907295586	18.513541925330145	37.276179483829544	27.233619517884446
3	21.48028610644518	21.373747905740192	26.729688248674222	30.41627773914041
4	14.084232230688794	13.458324518493425	33.64898031229842	38.808462938519355
5	15.699682330350475	35.59899031068628	32.440877187061645	16.260450171901596
6	36.61308344898381	32.079209848552864	15.903582658810727	15.404124043652601
7	32.573849299714794	29.443967883389767	18.407008518835262	19.575174298060173
8	29.134458297157824	32.97459478921254	18.140057888027645	19.750889025601992
9	27.207180987240875	15.985493758822065	18.024687941103206	38.782637312833856
10-11	27.750334348542005	24.27428797545336	25.21838879140435	22.75698888460029
12-13	27.5705407085818	21.074485016150323	26.0969188161423	25.258055459125572
14-15	24.270412692028668	22.79130136756739	23.428930066913388	29.509355873490556
16-17	25.126352190344285	26.14033750498008	23.888718606560786	24.84459169811484
18-19	25.138583701180963	25.29616006444526	25.347178508093947	24.21807772627983
20-21	25.442832814445186	24.57077183876814	25.342088058403395	24.64430728838327
22-23	25.228219503203487	24.20573986184936	25.314643454941116	25.25139718000604
24-25	25.024744683543894	24.50436534078432	25.404552589078154	25.06633738659363
26-27	25.034290718212844	24.657606718838615	25.442576922319592	24.865525640628945
28-29	25.026306707101348	24.853243952919488	25.36145080164321	24.758998538335952
30-31	25.090146811293828	24.805932070113442	25.231751233089835	24.872169885502895
32-33	24.94076375182802	24.767899446739065	25.244579310177777	25.04675749125514
34-35	25.07544686128359	24.779205263758232	25.37678933625755	24.768558538700628
36-37	25.162988730970508	24.72476259397067	25.24585228120047	24.866396393858352
38-39	25.080160724794503	24.812681077133135	25.273657565386177	24.83350063268618
40-41	25.156471925393113	24.760401429997884	25.27139139968354	24.81173524492546
42-43	25.093946586975953	24.679556742303944	25.25141451058928	24.975082160130828
44-45	25.023776168455242	24.76021712379647	25.20202136551708	25.013985342231216
46-47	25.120333390232663	24.788814207462057	25.250448503263307	24.84040389904197
48-49	25.158904928434794	24.89963770599788	25.027283138298053	24.914174227269275
50-51	24.84314951854997	25.077409271205404	25.127946963565922	24.951494246678703
52-53	25.037496356937478	25.040867549121533	25.06729334591912	24.85434274802187
54-55	24.975280519222707	24.80759573791034	25.178700510349238	25.038423232517715
56-57	24.894237316573875	25.015588653416366	25.175854572262345	24.914319457747414
58-59	25.10277295211896	24.86764295267305	25.137202624434096	24.89238147077389
60-61	25.059857229302708	24.798196791120084	25.261777246027417	24.880168733549787
62-63	24.87895749729975	25.009875642432956	25.246282493037057	24.864884367230232
64-65	24.934985801546862	24.92084495523949	25.19428175043895	24.949887492774693
66-67	24.96034662092544	24.97991645637248	25.098955416544488	24.960781506157595
68-69	24.93512596034172	24.95579523852423	25.199099375868215	24.909979425265835
70-71	24.84652944585071	24.806254931808667	25.262699424285163	25.08451619805546
72-73	25.04643821976526	24.849554285396565	25.168456305069537	24.93555118976864
74-75	24.770917723209458	25.13527352417334	25.12739098315481	24.966417769462385
76-77	25.010965020634004	24.91628414427068	25.041946158409502	25.03080467668582
78-79	24.931293519081347	24.80637856744354	25.255231953731084	25.00709595974403
80-81	24.912057711119314	25.03478656764534	25.124072543688293	24.929083177547056
82-83	25.072280654197755	24.92637266995212	25.058694432516226	24.942652243333903
84-85	24.933237476992197	25.00788730351495	25.02440015781121	25.034475061681643
86-87	24.979426245206152	24.96481290802804	25.175240620647756	24.88052022611805
88-89	24.911908192301915	24.770394419550815	25.09041037039338	25.22728701775389
90-91	24.94796029661898	24.814189675231642	25.115108425323257	25.12274160282612
92-93	25.03921973332644	24.983890295709568	25.15240838857682	24.824481582387172
94-95	25.084280981995192	24.78958122339056	25.102002058120572	25.02413573649368
96-97	24.992369576766944	24.807404425895207	25.18025551575635	25.0199704815815
98	25.02280047112288	24.994593031133718	24.913662216014703	25.0689442817287
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	393.0
1	279.5
2	235.5
3	330.5
4	327.0
5	346.5
6	536.0
7	929.0
8	1368.0
9	1459.0
10	1333.5
11	1377.5
12	1591.5
13	1937.5
14	2357.5
15	2712.5
16	3055.0
17	3303.5
18	3379.0
19	3357.0
20	3201.5
21	3061.5
22	3006.5
23	3101.5
24	3493.0
25	4146.5
26	5289.5
27	6938.0
28	9044.5
29	11632.5
30	15134.5
31	19676.5
32	24985.5
33	30686.0
34	37263.0
35	45418.0
36	54896.5
37	65137.0
38	75734.5
39	86623.5
40	98680.0
41	112202.5
42	126334.5
43	142527.5
44	157590.0
45	170916.0
46	187168.5
47	203845.5
48	211711.0
49	211821.5
50	211708.0
51	212996.0
52	217125.0
53	220752.5
54	219163.5
55	211935.0
56	199548.0
57	186671.5
58	173606.5
59	157131.0
60	138689.0
61	117418.0
62	95290.0
63	73855.5
64	55489.5
65	42528.5
66	32749.5
67	25206.5
68	19513.0
69	15199.5
70	11715.5
71	9231.5
72	7380.0
73	5664.0
74	4432.0
75	3471.0
76	2541.5
77	1641.5
78	988.0
79	618.5
80	397.5
81	252.0
82	135.5
83	76.0
84	57.5
85	37.5
86	21.0
87	21.0
88	15.0
89	10.5
90	13.5
91	11.0
92	8.5
93	9.5
94	8.5
95	6.5
96	4.5
97	3.5
98	2.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.46744843549304554
2	0.04754262567176389
3	0.055520446896357295
4	0.05268821226106804
5	0.06293614521623679
6	0.06308748599827514
7	0.041532234613669144
8	0.018982458089953934
9	0.011999162004469741
10-11	0.014226033511605568
12-13	0.010669525133704177
14-15	0.014874636863198527
16-17	0.014063882673707328
18-19	0.05372597762361677
20-21	0.08091326811122164
22-23	0.09723645245964443
24-25	0.06741150834222821
26-27	0.0823726256523058
28-29	0.08384279324924984
30-31	0.055909608907313064
32-33	0.03167346366945616
34-35	0.3950534913994114
36-37	0.6185405862466253
38-39	0.5682089661630118
40-41	0.5624580164455542
42-43	0.534092429869222
44-45	0.5205474298767891
46-47	0.5108508097704744
48-49	0.4998894131285534
50-51	0.5147099997124525
52-53	0.5954827370974957
54-55	0.5668252790129468
56-57	0.5236391058527157
58-59	0.5013271505579178
60-61	0.5134452231768463
62-63	0.5265902511024635
64-65	0.5442646924333717
66-67	0.5709547203514219
68-69	0.5251849438406788
70-71	0.4738155583945165
72-73	0.4559141058905508
74-75	0.43692083774473695
76-77	0.4520441058927128
78-79	0.44515810030996755
80-81	0.4422718153953789
82-83	0.4625406701326588
84-85	0.4285106142857482
86-87	0.4311266478038399
88-89	0.4578599159453297
90-91	0.44160159193206616
92-93	0.4362830444490039
94-95	0.44616343550493665
96-97	0.4410827092507918
98	0.43575335171186963
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	4625323.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.197530192723114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.8049922617975	36.0443326704983
2	14.370609108197819	14.427381691931638
3	5.537194560928136	8.33860473465517
4	2.65024310205173	5.321426325331916
5	1.4681915644579733	3.6849795192790262
6	0.9241980421128307	2.7835475473808646
7	0.6251853946076549	2.196793390530709
8	0.4288421839902354	1.7221454783010532
9	0.3195928656963808	1.4438495272655683
>10	1.7153133920530834	15.504530306416825
>50	0.10686604458025456	3.6940406436618
>100	0.04712639402789554	4.018334843441897
>500	0.0011255850302759397	0.3606974163474741
>1k	5.195004682189593E-4	0.45933590495767135
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	4.32402234395306E-5
6	0.0	0.0	0.0	0.0	8.64804468790612E-5
7	0.0	0.0	0.0	2.16201117197653E-5	8.64804468790612E-5
8	0.0	0.0	0.0	2.16201117197653E-5	8.64804468790612E-5
9	0.0	0.0	0.0	6.48603351592959E-5	8.64804468790612E-5
10-11	0.0	0.0	0.0	7.567039101917856E-5	8.64804468790612E-5
12-13	0.0	0.0	0.0	1.8377094961800506E-4	9.729050273894386E-5
14-15	0.0	2.16201117197653E-5	0.0	2.0539106133777037E-4	1.1891061445870916E-4
16-17	0.0	2.16201117197653E-5	0.0	3.459217875162448E-4	1.513407820383571E-4
18-19	0.0	4.32402234395306E-5	0.0	5.621229047138978E-4	1.513407820383571E-4
20-21	0.0	4.32402234395306E-5	0.0	5.621229047138978E-4	1.513407820383571E-4
22-23	0.0	4.32402234395306E-5	0.0	6.269832398731938E-4	1.513407820383571E-4
24-25	0.0	4.32402234395306E-5	0.0	6.918435750324897E-4	1.513407820383571E-4
26-27	0.0	4.32402234395306E-5	0.0	8.431843570708467E-4	1.513407820383571E-4
28-29	0.0	4.32402234395306E-5	0.0	0.0015242178762434537	1.513407820383571E-4
30-31	0.0	4.32402234395306E-5	0.0	0.0035673184337612746	1.513407820383571E-4
32-33	0.0	4.32402234395306E-5	0.0	0.007448128487459146	1.6215083789823976E-4
34-35	0.0	4.32402234395306E-5	0.0	0.011847821222431384	1.729608937581224E-4
36-37	0.0	4.32402234395306E-5	0.0	0.018193324012182502	1.729608937581224E-4
38-39	0.0	4.32402234395306E-5	0.0	0.02850611730251055	1.729608937581224E-4
40-41	0.0	4.32402234395306E-5	0.0	0.03960804467061003	1.729608937581224E-4
42-43	0.0	4.32402234395306E-5	0.0	0.04973706701132008	1.9458100547788771E-4
44-45	0.0	4.32402234395306E-5	0.0	0.0601903910278266	1.9458100547788771E-4
46-47	0.0	4.32402234395306E-5	0.0	0.07195173180337891	1.9458100547788771E-4
48-49	0.0	4.32402234395306E-5	0.0	0.08475083794147997	2.1620111719765302E-4
50-51	0.0	4.32402234395306E-5	0.0	0.09812287704015482	2.1620111719765302E-4
52-53	0.0	4.32402234395306E-5	0.0	0.11072740217277799	3.026815640767142E-4
54-55	0.0	4.32402234395306E-5	0.0	0.12328868708196161	3.134916199365969E-4
56-57	0.0	4.32402234395306E-5	0.0	0.13748229042598756	3.459217875162448E-4
58-59	0.0	4.32402234395306E-5	0.0	0.15205424572510934	3.6754189923601013E-4
60-61	0.0	4.32402234395306E-5	0.0	0.16638837979531373	3.6754189923601013E-4
62-63	0.0	4.32402234395306E-5	0.0	0.1803225417987025	4.1078212267554073E-4
64-65	0.0	4.32402234395306E-5	0.0	0.1936405306180779	4.1078212267554073E-4
66-67	0.0	4.32402234395306E-5	0.0	0.2086124579840154	4.1078212267554073E-4
68-69	0.0	6.48603351592959E-5	0.0	0.22433027920428475	4.1078212267554073E-4
70-71	0.0	6.48603351592959E-5	2.16201117197653E-5	0.24128044679258076	4.2159217853542335E-4
72-73	0.0	6.48603351592959E-5	2.16201117197653E-5	0.2571171786273089	4.864525136947193E-4
74-75	0.0	6.48603351592959E-5	2.16201117197653E-5	0.2740241059921653	5.188826812743672E-4
76-77	1.081005585988265E-5	8.64804468790612E-5	2.16201117197653E-5	0.2906067316812253	5.188826812743672E-4
78-79	2.16201117197653E-5	8.64804468790612E-5	2.16201117197653E-5	0.3080974020625154	5.188826812743672E-4
80-81	2.16201117197653E-5	8.64804468790612E-5	2.16201117197653E-5	0.32727444115794724	5.188826812743672E-4
82-83	2.16201117197653E-5	8.64804468790612E-5	2.16201117197653E-5	0.3464514802533791	5.837430164336631E-4
84-85	2.16201117197653E-5	8.64804468790612E-5	2.16201117197653E-5	0.36531502772887425	6.053631281534284E-4
86	2.16201117197653E-5	8.64804468790612E-5	2.16201117197653E-5	0.3806436869381879	6.053631281534284E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	15470	0.0	29.457708	1
GTACCTG	4305	0.0	29.190638	1
TACATGG	15835	0.0	28.167313	2
ACATGGG	16190	0.0	26.813517	3
GTATCAA	10450	0.0	25.460424	1
GAGTACA	11105	0.0	25.409492	1
TACCTGG	4820	0.0	25.202839	2
AGTACAT	10665	0.0	24.370974	2
CATGGGG	12855	0.0	22.501318	4
TCAACGC	12190	0.0	21.397312	4
CAACGCA	12485	0.0	21.001877	5
TATCAAC	12855	0.0	20.36173	2
ATCAACG	12815	0.0	20.317972	3
AACGCAG	13070	0.0	20.196684	6
ACCTGGG	6605	0.0	18.252972	3
ACGCAGA	15095	0.0	17.452969	7
GTGTAGC	2430	0.0	17.42752	1
ATGGGGG	6380	0.0	17.100426	5
GGTATCA	8000	0.0	16.743917	1
AGAGTAC	11615	0.0	16.505764	10-11
>>END_MODULE