FastQCFastQC Report
Mon 15 Aug 2016
SRR2921450_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921450_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4625323
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG43950.027.742381
GTATCAA105650.027.273981
GTACATG155950.026.997871
GAGTACA114200.026.650011
TACATGG160400.025.848432
AGTACAT108500.025.5189672
ACATGGG157650.025.1561953
TACCTGG49600.023.4589562
TCAACGC123300.022.9200614
CAACGCA125950.022.2761255
ATCAACG129000.021.906843
CATGGGG126850.021.4174354
TATCAAC133850.021.3600542
AACGCAG133350.021.0050336
GGTATCA77300.020.6408671
ACGCAGA153400.018.322287
AGAGTAC120200.017.95111710-11
GTATAAC8450.017.4730051
CGCAGAG163750.017.1062168
ACCTGGG66850.016.6962493
Produced by FastQC (version 0.11.5)