##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921450_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4625323
Sequences flagged as poor quality	0
Sequence length	101
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76218958113844	33.0	31.0	34.0	30.0	34.0
2	31.872566521300243	34.0	31.0	34.0	30.0	34.0
3	32.00196461955198	34.0	31.0	34.0	30.0	34.0
4	35.56471839912586	37.0	35.0	37.0	33.0	37.0
5	35.33951466740809	37.0	35.0	37.0	33.0	37.0
6	35.37406620034968	37.0	35.0	37.0	33.0	37.0
7	35.293243520506564	37.0	35.0	37.0	33.0	37.0
8	35.24126250209985	37.0	35.0	37.0	33.0	37.0
9	36.83251288612709	39.0	37.0	39.0	33.0	39.0
10-11	36.7311594238932	39.0	37.0	39.0	33.0	39.0
12-13	36.5914580452003	39.0	37.0	39.0	32.0	39.0
14-15	37.84060410483765	40.0	38.0	41.0	33.0	41.0
16-17	37.72674762389567	40.0	38.0	41.0	32.5	41.0
18-19	37.600737721452106	40.0	38.0	41.0	32.0	41.0
20-21	37.469418784374625	40.0	38.0	41.0	32.0	41.0
22-23	37.42949411316788	40.0	37.5	41.0	32.0	41.0
24-25	37.47789711118553	40.0	38.0	41.0	32.0	41.0
26-27	37.352216591144014	40.0	37.0	41.0	32.0	41.0
28-29	37.19902112782178	40.0	37.0	41.0	31.0	41.0
30-31	37.04384937008723	40.0	37.0	41.0	31.0	41.0
32-33	36.86686745985091	40.0	37.0	41.0	30.5	41.0
34-35	36.69946152949751	40.0	36.0	41.0	30.0	41.0
36-37	36.58523491224288	39.0	36.0	41.0	30.0	41.0
38-39	36.53906548796701	39.0	36.0	41.0	30.0	41.0
40-41	36.35343369533328	39.0	36.0	41.0	30.0	41.0
42-43	36.080785060848726	39.0	35.0	40.5	29.5	41.0
44-45	35.842936374389424	39.0	35.0	40.0	28.5	41.0
46-47	35.54065153936276	38.5	35.0	40.0	27.5	41.0
48-49	35.16525181052221	38.0	34.5	40.0	26.5	41.0
50-51	34.847022467403896	38.0	34.0	40.0	26.0	41.0
52-53	34.49264191927785	38.0	34.0	40.0	25.0	41.0
54-55	34.12288482339504	37.5	33.0	40.0	24.0	41.0
56-57	33.69263249290914	37.0	33.0	40.0	23.0	41.0
58-59	33.254763937567176	36.5	32.5	39.0	22.0	40.5
60-61	32.7791022378329	36.0	32.0	39.0	20.5	40.0
62-63	32.296682523577275	35.5	31.0	39.0	19.0	40.0
64-65	31.73161593255217	35.0	31.0	38.0	17.5	40.0
66-67	31.155342880918802	35.0	30.0	37.5	13.0	40.0
68-69	30.606926370331326	34.0	29.5	37.0	8.0	39.0
70-71	30.01357288993655	34.0	29.0	36.0	7.0	39.0
72-73	29.427667754230356	34.0	28.5	36.0	6.5	38.5
74-75	28.87469069727671	33.5	27.5	35.0	2.0	37.5
76-77	27.72095365015589	31.5	26.5	34.0	2.0	36.0
78-79	28.65681121080625	33.0	28.5	35.0	2.0	36.0
80-81	28.664041300467016	34.0	29.0	35.0	2.0	36.0
82-83	28.29309596324408	33.5	27.5	35.0	2.0	36.0
84-85	27.75221665600435	33.0	26.5	35.0	2.0	35.0
86-87	27.176149319733995	33.0	26.0	35.0	2.0	35.0
88-89	26.583329423696465	32.0	25.0	34.0	2.0	35.0
90-91	26.028738209201823	32.0	24.0	34.0	2.0	35.0
92-93	25.510841296921317	32.0	22.5	34.0	2.0	35.0
94-95	24.91805815507371	31.0	19.5	34.0	2.0	35.0
96-97	24.03241135808245	31.0	9.0	34.0	2.0	35.0
98-99	22.899008890838545	31.0	2.0	34.0	2.0	35.0
100-101	19.379217083866358	25.0	2.0	32.0	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	36798.0
3	18960.0
4	11529.0
5	8561.0
6	7511.0
7	7911.0
8	9329.0
9	10903.0
10	12957.0
11	15510.0
12	17902.0
13	20888.0
14	23270.0
15	26321.0
16	29798.0
17	34223.0
18	35186.0
19	32938.0
20	34043.0
21	35470.0
22	38170.0
23	41973.0
24	47046.0
25	53968.0
26	63662.0
27	76814.0
28	91978.0
29	112104.0
30	136421.0
31	168339.0
32	211862.0
33	279572.0
34	384866.0
35	556397.0
36	796022.0
37	838192.0
38	290876.0
39	7053.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.08297539286704	16.702514930270464	11.780828097057928	25.433681579804567
2	17.12578775579565	18.82865261517952	36.87699215816928	27.16856747085555
3	21.47668376418015	21.627731139132017	26.98532920479157	29.910255891896263
4	14.400724599589287	13.836377988491986	33.51435343612628	38.24854397579245
5	15.73875948830141	35.567069040479744	32.480242907434565	16.21392856378428
6	36.67856584247874	32.2689985330287	15.668252910956939	15.384182713535619
7	32.602591061338174	29.500989513771952	18.391325597954292	19.50509382693559
8	29.181356183837657	33.0538916453601	18.05989297591114	19.7048591948911
9	27.090050158304102	16.016445189518063	18.04898284395139	38.844521808226446
10-11	27.753098191020438	24.344952816520568	25.218724160101175	22.683224832357823
12-13	27.537073192477635	21.11015508784474	26.137579923280573	25.21519179639705
14-15	24.181297262180554	22.8179311721924	23.503221635336214	29.497549930290834
16-17	25.028818749207886	26.15150638251488	24.018291741335883	24.801383126941346
18-19	25.052897150377575	25.399456483537158	25.417808004377545	24.12983836170772
20-21	25.409401504508022	24.536062810057835	25.47900519383876	24.575530491595384
22-23	25.152708908912786	24.239067158178667	25.48606599810681	25.122157934801738
24-25	24.98438232796473	24.471294356540557	25.537762108981017	25.006561206513688
26-27	24.914372138394604	24.70091608134382	25.59863817154809	24.786073608713487
28-29	24.906028085827465	24.86862745861278	25.53604321907923	24.68930123648052
30-31	24.96757962612466	24.81169373373306	25.391476825324343	24.82924981481794
32-33	24.814335503423564	24.738574594387792	25.430833414322983	25.01625648786566
34-35	25.018127111293598	24.74864477748383	25.551837700434714	24.681390410787856
36-37	24.963850018687175	24.778556701387586	25.465457699575733	24.792135580349502
38-39	24.953687856477007	24.804658654581146	25.50771451004271	24.733938978899133
40-41	25.010393227843014	24.766913282150536	25.452460862266513	24.770232627739933
42-43	24.970730799242148	24.752576608552033	25.404779803721766	24.871912788484053
44-45	24.834664128975923	24.741663039386317	25.412099050376924	25.011573781260832
46-47	24.952646119079414	24.841546421292215	25.432412998934957	24.773394460693414
48-49	25.022707121268894	24.934981204060104	25.22526048057155	24.817051194099456
50-51	24.77424022325653	25.07330010301636	25.276889578331303	24.875570095395812
52-53	25.009659014622677	25.085277153722433	25.095817208850708	24.80924662280418
54-55	24.97333860165087	24.857687860834258	25.20492115631282	24.964052381202052
56-57	24.836313664595604	25.07586228964768	25.259315521613644	24.82850852414307
58-59	25.08388153520884	24.915664437536986	25.168114401031033	24.832339626223142
60-61	25.021821324379793	24.894450086409634	25.296941901115726	24.786786688094846
62-63	24.818580473290893	25.010627854276223	25.327517385137003	24.843274287295877
64-65	24.925283985718956	24.94150015810768	25.208580517349954	24.924635338823407
66-67	24.941703842869266	25.029341380760556	25.133535319257906	24.895419457112272
68-69	24.923743883285958	25.0345045407543	25.203944550445854	24.837807025513893
70-71	24.890689146778016	24.89527315901906	25.282503268541273	24.931534425661646
72-73	25.078616173678025	24.880211778417284	25.139690297529814	24.901481750374874
74-75	24.743957022330704	25.2160585691011	25.06434061308417	24.97564379548402
76-77	24.926559925301138	24.969343498750863	25.132085880271482	24.972010695676516
78-79	24.887669882568712	24.88909784216914	25.310313470667705	24.91291880459444
80-81	24.816963518141442	25.13196382632918	25.19817552859604	24.85289712693334
82-83	24.992138993834462	25.01533274655121	25.106431751977148	24.886096507637177
84-85	24.85438005904483	25.091024288821217	25.139776005916374	24.91481964621758
86-87	24.849861606768417	25.0961393293813	25.228563930097213	24.825435133753068
88-89	24.80773677475886	24.914929988108238	25.16055620075523	25.11677703637767
90-91	24.83875988525185	24.965916283725452	25.20218603366419	24.993137797358507
92-93	24.96879858660301	25.16448606459439	25.20947666415469	24.657238684647908
94-95	24.933474798262203	24.97046988079194	25.1205583468236	24.97549697412225
96-97	24.8343589056142	24.976843543651324	25.28794221047943	24.900855340255045
98-99	24.880693453691805	25.041120243657577	25.16887571080668	24.90931059184393
100-101	25.44518478694363	24.35020269898463	25.28182126489053	24.92279124918121
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	45.5
2	114.5
3	204.0
4	169.0
5	144.5
6	309.5
7	665.5
8	1035.5
9	1088.0
10	904.0
11	772.5
12	756.0
13	854.5
14	960.0
15	1069.0
16	1201.0
17	1327.5
18	1473.0
19	1580.5
20	1673.5
21	1851.5
22	2098.0
23	2421.0
24	2845.5
25	3458.5
26	4397.5
27	5752.0
28	7585.0
29	9996.5
30	13107.0
31	16761.5
32	21253.0
33	26682.5
34	32394.5
35	38735.0
36	47803.5
37	57812.5
38	67768.0
39	78425.5
40	89557.5
41	101415.5
42	114261.0
43	129082.0
44	145056.0
45	161330.5
46	174742.5
47	187512.5
48	204282.0
49	212206.5
50	209603.0
51	209871.5
52	213187.0
53	215815.5
54	216340.0
55	210775.5
56	199055.0
57	184339.0
58	170277.5
59	155582.0
60	136799.0
61	116875.5
62	95996.5
63	73770.5
64	55270.0
65	42468.0
66	32713.5
67	25360.5
68	19622.5
69	15380.5
70	12114.5
71	9521.5
72	7350.0
73	5668.5
74	4385.5
75	3426.0
76	2594.0
77	1633.0
78	988.0
79	623.0
80	403.5
81	262.5
82	136.0
83	65.5
84	40.0
85	16.5
86	9.0
87	6.5
88	3.0
89	2.5
90	2.0
91	1.5
92	1.5
93	0.5
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30745960876678236
2	0.0
3	0.0061401117284133454
4	0.05690413404642227
5	4.756424578348366E-4
6	0.06213620108260548
7	0.07255709493153235
8	0.10120374296022137
9	0.08980994408390505
10-11	0.0933988826293861
12-13	0.09359346363486398
14-15	0.06277399437833855
16-17	0.03452731841646518
18-19	0.03735955305175444
20-21	0.02781427372747806
22-23	0.04113226254685349
24-25	0.03360846366837516
26-27	0.020582346357216567
28-29	0.023111899428429106
30-31	0.0031349161993659684
32-33	0.005329357538922146
34-35	0.007448128487459147
36-37	0.010669525133704177
38-39	0.01569620110854961
40-41	0.019836452502884662
42-43	0.0033294972048438566
44-45	0.013966592170968383
46-47	0.0237388826683023
48-49	0.002637653629811367
50-51	0.0071238268116626665
52-53	0.002378212289174183
54-55	0.00411863128261529
56-57	0.00358893854548104
58-59	0.0010810055859882652
60-61	0.005621229047138979
62-63	0.0147665363045997
64-65	0.0067130446889871265
66-67	0.03747846366621315
68-69	0.05930396644731622
70-71	0.011966731836890093
72-73	0.03099243015028356
74-75	0.049099273715587
76-77	0.29706033502957524
78-79	0.07228684353503528
80-81	0.03281932959060373
82-83	0.026657597750470614
84-85	0.018820307252055694
86-87	0.11535410608080776
88-89	0.5149694410530897
90-91	0.12572094965043523
92-93	0.04615893852169892
94-95	0.008302122900389876
96-97	0.005686089382298274
98-99	0.010204692731729222
100-101	0.007815670386695155
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	4625323.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.343514960039776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.80161835063976	30.290201810371016
2	16.53451168070639	14.994657555021254
3	6.55855097925618	8.921632633322583
4	3.2568350810323574	5.907054008766923
5	1.7833961790110449	4.043272566133255
6	1.1040186470276925	3.003605162259783
7	0.7445995150737922	2.3633931475690804
8	0.5414691251557209	1.9641690701518464
9	0.4002710559672067	1.6334726952887961
>10	2.072651019776329	16.895458180271277
>50	0.1387537360179424	4.301549181829078
>100	0.061123576994650194	4.713418607351824
>500	0.0015790168405167716	0.45506058607325983
>1k	6.220365004280011E-4	0.5130547955900746
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.16201117197653E-5
2	0.0	0.0	0.0	0.0	2.16201117197653E-5
3	0.0	0.0	0.0	0.0	2.16201117197653E-5
4	0.0	0.0	0.0	2.16201117197653E-5	2.16201117197653E-5
5	0.0	0.0	0.0	2.16201117197653E-5	2.16201117197653E-5
6	0.0	0.0	0.0	2.16201117197653E-5	2.16201117197653E-5
7	0.0	0.0	0.0	2.16201117197653E-5	2.16201117197653E-5
8	0.0	0.0	0.0	2.16201117197653E-5	2.16201117197653E-5
9	0.0	0.0	0.0	1.0810055859882651E-4	2.16201117197653E-5
10-11	0.0	0.0	0.0	1.297206703185918E-4	2.16201117197653E-5
12-13	0.0	0.0	0.0	1.9458100547788771E-4	9.729050273894386E-5
14-15	0.0	0.0	0.0	1.9458100547788771E-4	1.0810055859882651E-4
16-17	0.0	0.0	0.0	2.4863128477730094E-4	1.0810055859882651E-4
18-19	0.0	0.0	0.0	3.243016757964795E-4	1.297206703185918E-4
20-21	0.0	0.0	0.0	3.243016757964795E-4	1.513407820383571E-4
22-23	0.0	0.0	0.0	3.243016757964795E-4	1.513407820383571E-4
24-25	0.0	0.0	0.0	3.6754189923601013E-4	1.513407820383571E-4
26-27	0.0	0.0	0.0	4.6483240197495396E-4	1.9458100547788771E-4
28-29	0.0	0.0	0.0	0.0011891061445870915	1.9458100547788771E-4
30-31	0.0	0.0	0.0	0.003178156422805499	2.0539106133777037E-4
32-33	0.0	0.0	0.0	0.007080586588223136	2.3782122891741832E-4
34-35	0.0	0.0	0.0	0.011534329602494788	2.3782122891741832E-4
36-37	0.0	0.0	0.0	0.017814972057086608	2.3782122891741832E-4
38-39	0.0	0.0	0.0	0.027814273727478057	2.594413406371836E-4
40-41	0.0	0.0	0.0	0.03875405025767931	2.594413406371836E-4
42-43	0.0	0.0	0.0	0.04876416198393063	2.702513964970663E-4
44-45	0.0	0.0	0.0	0.05896885471565985	2.9187150821683154E-4
46-47	0.0	0.0	0.0	0.07041670387127558	3.026815640767142E-4
48-49	0.0	0.0	0.0	0.08289150833358017	3.243016757964795E-4
50-51	0.0	0.0	0.0	0.095896005533019	3.243016757964795E-4
52-53	0.0	0.0	0.0	0.10803569826366721	3.459217875162448E-4
54-55	0.0	0.0	0.0	0.12004567032399684	3.7835195509589275E-4
56-57	0.0	0.0	0.0	0.13393659210394604	4.1078212267554073E-4
58-59	0.0	0.0	0.0	0.14794642449835396	4.432122902551887E-4
60-61	0.0	0.0	0.0	0.16196706694862176	4.5402234611507133E-4
62-63	0.0	0.0	0.0	0.17546882671761518	4.5402234611507133E-4
64-65	0.0	0.0	0.0	0.1882355026881366	4.5402234611507133E-4
66-67	0.0	0.0	0.0	0.2023210054735637	4.5402234611507133E-4
68-69	0.0	0.0	0.0	0.2173253630070808	4.5402234611507133E-4
70-71	0.0	0.0	0.0	0.2337242177465228	4.6483240197495396E-4
72-73	0.0	0.0	0.0	0.248901536173798	5.080726254144846E-4
74-75	0.0	0.0	0.0	0.26497608923744354	5.837430164336631E-4
76-77	0.0	0.0	0.0	0.2809425417424902	6.702234633127243E-4
78-79	0.0	2.16201117197653E-5	0.0	0.2977197484370281	6.918435750324897E-4
80-81	0.0	2.16201117197653E-5	0.0	0.31651843557736403	7.458938543319028E-4
82-83	0.0	2.16201117197653E-5	0.0	0.3346901394778268	8.648044687906121E-4
84-85	0.0	2.16201117197653E-5	0.0	0.35293751376930865	8.648044687906121E-4
86-87	0.0	2.16201117197653E-5	0.0	0.3720388824737213	8.864245805103774E-4
88-89	0.0	3.243016757964795E-5	0.0	0.3921672064848228	9.188547480900252E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTG	4395	0.0	27.74238	1
GTATCAA	10565	0.0	27.27398	1
GTACATG	15595	0.0	26.99787	1
GAGTACA	11420	0.0	26.65001	1
TACATGG	16040	0.0	25.84843	2
AGTACAT	10850	0.0	25.518967	2
ACATGGG	15765	0.0	25.156195	3
TACCTGG	4960	0.0	23.458956	2
TCAACGC	12330	0.0	22.920061	4
CAACGCA	12595	0.0	22.276125	5
ATCAACG	12900	0.0	21.90684	3
CATGGGG	12685	0.0	21.417435	4
TATCAAC	13385	0.0	21.360054	2
AACGCAG	13335	0.0	21.005033	6
GGTATCA	7730	0.0	20.640867	1
ACGCAGA	15340	0.0	18.32228	7
AGAGTAC	12020	0.0	17.951117	10-11
GTATAAC	845	0.0	17.473005	1
CGCAGAG	16375	0.0	17.106216	8
ACCTGGG	6685	0.0	16.696249	3
>>END_MODULE