FastQCFastQC Report
Mon 15 Aug 2016
SRR2921449_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921449_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3107619
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63770.20520533566051694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39640.12755746441246496No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35560.11442844183923448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88800.029.9583991
GTACATG94350.024.927731
TATCAAC107750.024.6339512
TCAACGC106600.024.598674
CAACGCA109850.023.87095
ATCAACG111600.023.5787373
TACATGG97750.023.4021952
GGTATCA61050.022.9187891
AACGCAG116800.022.5221066
ACATGGG102700.021.2029343
ACGCAGA130450.020.0922687
CATGGGG77000.019.82564
CGCAGAG136550.019.2580958
GTATAGC6550.018.9725721
GAGTACA75750.018.7142241
GTACCTG29200.018.4419461
AGAGTAC103700.018.28960210-11
TAGGACG2353.5508874E-617.5568874
GAGTACT54250.017.52777712-13
GTATAAG9800.017.3771841
Produced by FastQC (version 0.11.5)