##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921449_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3107619
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.791972568065777	31.0	31.0	34.0	28.0	34.0
2	30.90687693697329	31.0	31.0	34.0	27.0	34.0
3	31.035948100458903	33.0	31.0	34.0	28.0	34.0
4	34.56447588974066	37.0	35.0	37.0	32.0	37.0
5	34.568128847197805	37.0	35.0	37.0	32.0	37.0
6	34.669413142344666	37.0	35.0	37.0	32.0	37.0
7	34.56931721681455	37.0	35.0	37.0	32.0	37.0
8	34.56568421032308	37.0	35.0	37.0	32.0	37.0
9	36.07417962111829	39.0	37.0	39.0	32.0	39.0
10-11	35.96531749870238	39.0	37.0	39.0	32.0	39.0
12-13	35.82648612973469	39.0	37.0	39.0	31.0	39.0
14-15	36.90677621677561	40.0	37.0	41.0	31.0	41.0
16-17	36.77976450781129	40.0	37.0	41.0	31.0	41.0
18-19	36.62347829640635	40.0	37.0	41.0	30.0	41.0
20-21	36.52539629214521	40.0	37.0	41.0	30.0	41.0
22-23	36.569793948357244	40.0	37.0	41.0	30.0	41.0
24-25	36.45176290915971	40.0	37.0	41.0	30.0	41.0
26-27	36.2068979498452	39.5	36.0	41.0	29.0	41.0
28-29	35.998413093754415	39.0	36.0	41.0	28.5	41.0
30-31	35.76054030432945	39.0	36.0	40.0	27.5	41.0
32-33	35.49708957243472	39.0	35.0	40.0	27.0	41.0
34-35	35.08886481901418	38.5	35.0	40.0	25.5	41.0
36-37	34.723053244300544	38.0	34.5	40.0	24.0	41.0
38-39	34.51626727729493	38.0	34.0	40.0	24.0	41.0
40-41	34.26447901110143	38.0	34.0	40.0	23.0	41.0
42-43	33.98654693512944	38.0	33.0	40.0	21.5	41.0
44-45	33.677689092517454	38.0	33.0	40.0	19.5	41.0
46-47	33.390258587040435	38.0	33.0	40.0	18.0	41.0
48-49	33.179423700266995	38.0	33.0	40.0	15.5	41.0
50-51	32.38945073382548	36.5	31.0	39.0	15.0	40.0
52-53	32.87258251413703	37.0	32.5	39.0	17.5	40.0
54-55	33.31908979189534	38.0	33.0	40.0	17.5	41.0
56-57	33.177532059110206	37.0	33.0	40.0	15.0	41.0
58-59	32.85854958410282	37.0	32.5	40.0	12.5	41.0
60-61	32.41460198306163	36.0	32.0	39.0	8.0	41.0
62-63	31.944011958994974	36.0	31.0	39.0	7.0	40.0
64-65	31.421752473517508	35.0	31.0	38.5	2.0	40.0
66-67	30.94974110404139	35.0	30.5	38.0	2.0	40.0
68-69	30.44318721181715	35.0	30.0	37.5	2.0	39.5
70-71	29.93519829811827	34.0	29.0	37.0	2.0	39.0
72-73	29.400189341100052	34.0	29.0	36.0	2.0	39.0
74-75	28.82911273872376	34.0	28.5	35.5	2.0	38.0
76-77	28.27370070140516	33.5	27.0	35.0	2.0	37.0
78-79	27.732073655103797	33.0	26.0	35.0	2.0	36.5
80-81	27.17533198245988	33.0	26.0	35.0	2.0	36.0
82-83	26.576890056342172	32.5	25.0	35.0	2.0	35.5
84-85	25.982508473529094	32.0	23.5	34.0	2.0	35.0
86-87	25.393600052001226	31.5	21.0	34.0	2.0	35.0
88-89	24.768006148758907	31.0	19.0	34.0	2.0	35.0
90-91	24.04870561674388	31.0	13.5	34.0	2.0	35.0
92-93	23.2568802674974	30.0	4.5	34.0	2.0	35.0
94-95	22.361698779676658	30.0	2.0	34.0	2.0	35.0
96-97	21.471590468458327	29.0	2.0	34.0	2.0	35.0
98	20.84190726083217	29.0	2.0	34.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	71682.0
3	12973.0
4	10060.0
5	8800.0
6	8296.0
7	8572.0
8	9786.0
9	11480.0
10	13034.0
11	14484.0
12	16307.0
13	19999.0
14	16299.0
15	15806.0
16	18153.0
17	21641.0
18	24669.0
19	22654.0
20	23197.0
21	25195.0
22	27694.0
23	30634.0
24	35039.0
25	39451.0
26	45184.0
27	52501.0
28	61445.0
29	72000.0
30	88420.0
31	107117.0
32	136014.0
33	179166.0
34	246870.0
35	356705.0
36	491310.0
37	525964.0
38	229242.0
39	9776.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.074125213379354	17.608036483139678	13.982519627343212	24.335318676137764
2	16.059766344744368	19.251301259582572	38.335797894997114	26.353134500675946
3	19.872747188634374	22.559410081699124	28.132910638402002	29.4349320912645
4	14.144037080956503	15.573499454095218	34.682356183631356	35.600107281316916
5	15.769673773636367	35.52280551259474	32.73037919616634	15.977141517602558
6	34.607576228371975	33.33505064181722	16.72199083279265	15.335382297018164
7	30.620741252969008	30.55410149974857	19.613495869944956	19.21166137733746
8	27.723271314730653	32.74681353639928	19.363231906573958	20.166683242296113
9	26.957656257844594	16.31871541138169	19.356688709837297	37.36693962093642
10-11	26.386341451483077	24.925132428303336	26.208735134703698	22.479790985509887
12-13	26.72228008805762	22.289581880764985	26.54907413501214	24.43906389616525
14-15	24.003162395239222	23.815369028448522	24.41318595872054	27.768282617591716
16-17	24.152181126352875	26.622508712887154	25.062524560059142	24.16278560070083
18-19	24.07339615546094	26.052927449328894	26.486391114751477	23.387285280458688
20-21	24.780275281285157	25.147947145297618	26.41739975333957	23.654377820077656
22-23	24.64575109882668	24.781132536752775	26.265078015043347	24.3080383493772
24-25	24.469083862844684	25.01053387463844	26.27409970994612	24.246282552570754
26-27	24.356227132623175	25.267371476908266	26.27088307506949	24.105518315399067
28-29	24.30436178002352	25.35085661172137	26.219183548520235	24.125598059734877
30-31	24.559332416240114	25.152330578565618	25.994875761773635	24.293461243420634
32-33	24.477818050718152	25.22708251517746	25.907090755750694	24.388008678353685
34-35	24.685158967841954	25.191499247703163	25.975203042597627	24.148138741857252
36-37	24.690941801049476	25.077520603744986	25.961746948200577	24.269790647004957
38-39	24.58427331443094	25.236022314513317	25.907920991901452	24.271783379154293
40-41	24.78513576105459	25.13849288026026	25.864999907752335	24.211371450932816
42-43	24.68926010705528	25.128981486335277	25.732534592989957	24.449223813619486
44-45	24.663418592319218	25.124908722726413	25.70249933918004	24.509173345774325
46-47	24.81437179987982	25.08226194259982	25.708363358153854	24.395002899366506
48-49	24.795553246726364	25.20894293216851	25.498412751810278	24.49709106929485
50-51	24.588634146475602	25.42454088376065	25.513425832991082	24.473399136772667
52-53	24.789965893336216	25.3555718451336	25.41890261631201	24.43555964521817
54-55	24.69230370847508	25.219669775135056	25.499172981248847	24.588853535141013
56-57	24.74966747972767	25.30347429086877	25.452060946250292	24.494797283153265
58-59	24.786222768886397	25.22208232147897	25.47624881630224	24.515446093332397
60-61	24.826529217515223	25.1581767875817	25.596227211664523	24.41906678323856
62-63	24.6353330656483	25.318916736257226	25.52362784187515	24.522122356219324
64-65	24.666788127829353	25.28604589225575	25.49878630526469	24.548379674650203
66-67	24.7210547858937	25.208710208863156	25.431319325607994	24.63891567963515
68-69	24.584680900077522	25.210450129353468	25.463947979070493	24.74092099149852
70-71	24.66100968682343	25.075032054961525	25.55696405155311	24.706994206661932
72-73	24.79585837127385	25.08125492359168	25.423523860131443	24.69936284500303
74-75	24.564513290112902	25.395524162350252	25.3397624681128	24.700200079424047
76-77	24.735719988548606	25.096332175746877	25.33226255373498	24.835685281969536
78-79	24.698577840221926	25.09759143529482	25.429216426849017	24.774614297634237
80-81	24.673363617672504	25.195516314362305	25.4097663761077	24.721353691857495
82-83	24.82175918590913	25.088691982412925	25.34784846766251	24.741700364015433
84-85	24.75260677814843	25.163637256915788	25.26490399618981	24.818851968745975
86-87	24.725382441122903	25.13800979826335	25.41552436288904	24.72108339772471
88-89	24.618625579338474	25.042375833755138	25.35115157995376	24.987847006952627
90-91	24.702493904729515	24.944300445273168	25.37520422631282	24.9780014236845
92-93	24.764813572986302	25.05485191558648	25.38688076328976	24.79345374813746
94-95	24.775988784446678	24.995033467719317	25.373104170771764	24.855873577062233
96-97	24.78288691984309	24.901931594812336	25.408174530323098	24.90700695502148
98	24.769723454799532	25.000137382041355	25.176535923141053	25.053603240018063
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1074.0
1	826.0
2	836.5
3	1050.5
4	885.0
5	827.5
6	1289.0
7	2690.5
8	3936.5
9	3763.5
10	2747.0
11	1985.0
12	1886.0
13	2074.5
14	2278.5
15	2449.0
16	2585.0
17	2731.0
18	2831.5
19	2820.5
20	2736.5
21	2539.5
22	2425.0
23	2457.0
24	2573.0
25	2959.0
26	3601.0
27	4537.5
28	5822.5
29	7793.0
30	10507.0
31	13987.0
32	18653.5
33	23529.0
34	28870.0
35	35515.0
36	42982.0
37	50518.0
38	58534.0
39	66555.5
40	74835.0
41	83870.0
42	93087.5
43	102280.5
44	110753.0
45	120018.0
46	130278.0
47	139313.0
48	143914.0
49	143515.5
50	142443.0
51	142373.0
52	142638.0
53	143350.0
54	140566.0
55	134587.5
56	126828.0
57	117377.5
58	107796.5
59	96582.5
60	84077.0
61	70754.5
62	57165.0
63	43803.0
64	32320.0
65	24571.0
66	18825.5
67	14136.5
68	10626.0
69	8073.5
70	5988.0
71	4379.5
72	3284.0
73	2339.0
74	1767.0
75	1285.0
76	832.0
77	504.0
78	295.0
79	177.5
80	102.5
81	60.0
82	34.5
83	24.5
84	19.5
85	13.0
86	10.0
87	8.5
88	8.5
89	7.0
90	5.5
91	4.5
92	5.0
93	8.0
94	5.0
95	4.0
96	5.0
97	2.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.48661048860880307
2	0.05116457326332475
3	0.058984064648851746
4	0.05682807319687517
5	0.06680355603437874
6	0.06384309016002283
7	0.04392430346191087
8	0.020851977028071976
9	0.012324548150851182
10-11	0.014995403233150526
12-13	0.010796046748330474
14-15	0.015461998398130531
16-17	0.014158749833876032
18-19	0.05492951356005996
20-21	0.0817506908021865
22-23	0.09838722185699082
24-25	0.06955485855891601
26-27	0.08438936690759066
28-29	0.08632010552130104
30-31	0.05764863710770207
32-33	0.03343395699408454
34-35	0.410716371601538
36-37	0.6400881189103297
38-39	0.5869284490795043
40-41	0.5827612715715794
42-43	0.5551838883724163
44-45	0.5393840107168865
46-47	0.528587963968556
48-49	0.5182424228967579
50-51	0.5323850832421864
52-53	0.6137496263216308
54-55	0.5859469902842015
56-57	0.5415721811457582
58-59	0.5194169555534317
60-61	0.532127651427025
62-63	0.5445326470201142
64-65	0.5621023684048785
66-67	0.5904037785841829
68-69	0.542634087383299
70-71	0.4915016930968693
72-73	0.4739802401774478
74-75	0.45364312677969854
76-77	0.46805930842873594
78-79	0.46228318207605246
80-81	0.45922617927101106
82-83	0.4792415028998085
84-85	0.4443755814338888
86-87	0.44752912116961574
88-89	0.4749456094843029
90-91	0.4581320940565752
92-93	0.45251686258836754
94-95	0.4638438624554683
96-97	0.45830907842949864
98	0.4523076992385489
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3107619.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.61664017359705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.09654797765748	44.01907331085615
2	14.170383502303654	16.612405417528183
3	4.75099689929676	8.354624271358604
4	2.0317127661133894	4.763687045894883
5	1.0734054266972792	3.145970982855041
6	0.6641583259288973	2.3358437765563655
7	0.42822293141886925	1.7570692639543948
8	0.29431853578104905	1.3801570966638157
9	0.23793097058060864	1.2552042679810418
>10	1.1844419173980536	12.214959623927856
>50	0.05432564559265735	2.1341490327923758
>100	0.01256482995210775	1.1651252218572843
>500	6.051657822550592E-4	0.23676471512520006
>1k	3.300904259536303E-4	0.4193205646208697
>5k	5.5015070992271714E-5	0.20564540802793865
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6377	0.20520533566051694	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3964	0.12755746441246496	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3556	0.11442844183923448	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.21789768951728E-5
7	0.0	0.0	0.0	0.0	3.21789768951728E-5
8	0.0	0.0	0.0	0.0	3.21789768951728E-5
9	0.0	0.0	0.0	3.21789768951728E-5	6.43579537903456E-5
10-11	0.0	0.0	0.0	9.65369306855184E-5	6.43579537903456E-5
12-13	0.0	4.826846534275919E-5	0.0	9.65369306855184E-5	2.4134232671379599E-4
14-15	0.0	9.653693068551838E-5	0.0	9.65369306855184E-5	2.574318151613824E-4
16-17	0.0	1.4480539602827758E-4	0.0	1.287159075806912E-4	2.574318151613824E-4
18-19	0.0	1.60894884475864E-4	0.0	2.252528382662096E-4	2.574318151613824E-4
20-21	0.0	1.60894884475864E-4	0.0	2.252528382662096E-4	3.057002805041416E-4
22-23	0.0	1.60894884475864E-4	0.0	3.21789768951728E-4	3.21789768951728E-4
24-25	0.0	1.60894884475864E-4	0.0	4.987741418751784E-4	3.378792573993144E-4
26-27	0.0	1.60894884475864E-4	0.0	7.722954454841473E-4	4.0223721118966E-4
28-29	0.0	1.60894884475864E-4	0.0	0.001769843729234504	4.183266996372464E-4
30-31	0.0	1.60894884475864E-4	0.0	0.005148636303227648	4.3441618808483283E-4
32-33	0.0	1.60894884475864E-4	0.0	0.012517622012222219	4.6659516498000556E-4
34-35	0.0	1.769843729234504E-4	0.0	0.022734447176439583	5.631320956655239E-4
36-37	0.0	2.252528382662096E-4	0.0	0.03591173821501284	5.792215841131104E-4
38-39	0.0	2.574318151613824E-4	0.0	0.05621667263586688	5.792215841131104E-4
40-41	0.0	2.574318151613824E-4	0.0	0.07882240390472578	5.792215841131104E-4
42-43	0.0	2.574318151613824E-4	0.0	0.10247395192267778	5.953110725606969E-4
44-45	0.0	2.574318151613824E-4	0.0	0.12746092748177945	6.43579537903456E-4
46-47	0.0	2.574318151613824E-4	0.0	0.1546360734697529	6.918480032462153E-4
48-49	0.0	2.574318151613824E-4	0.0	0.18068495526639528	7.401164685889743E-4
50-51	0.0	2.574318151613824E-4	0.0	0.20742568506628387	7.401164685889743E-4
52-53	0.0	2.574318151613824E-4	0.0	0.23287925579036556	8.205639108269064E-4
54-55	0.0	4.0223721118966E-4	0.0	0.2607462497815852	8.688323761696656E-4
56-57	0.0	4.183266996372464E-4	0.0	0.28933727075294624	8.688323761696656E-4
58-59	0.0	4.505056765324192E-4	0.0	0.3193280772192473	8.688323761696656E-4
60-61	0.0	4.8268465342759197E-4	0.0	0.34873966210143525	9.010113530648384E-4
62-63	0.0	4.8268465342759197E-4	0.0	0.3790200793597928	9.814587953027704E-4
64-65	0.0	4.8268465342759197E-4	0.0	0.41020150797121524	0.0010297272606455295
66-67	0.0	6.114005610082832E-4	0.0	0.44305624338118665	0.0010458167490931159
68-69	0.0	6.274900494558696E-4	0.0	0.4768602586095657	0.0010619062375407024
70-71	0.0	6.43579537903456E-4	0.0	0.5127880863130262	0.0011262641913310479
72-73	0.0	6.43579537903456E-4	0.0	0.5466886384720907	0.0012710695873593258
74-75	0.0	6.43579537903456E-4	0.0	0.5831313298058739	0.001367606518044844
76-77	0.0	6.43579537903456E-4	0.0	0.6211347015190729	0.0014158749833876032
78-79	0.0	6.596690263510423E-4	0.0	0.6618732862683617	0.001448053960282776
80-81	0.0	6.757585147986288E-4	0.0	0.701308622453396	0.0014641434487303623
82-83	0.0	6.757585147986288E-4	0.0	0.7428034131597213	0.0015124119140731216
84-85	0.0	6.757585147986288E-4	0.0	0.7849096044270549	0.0015124119140731216
86	0.0	6.757585147986288E-4	0.0	0.8172816551835987	0.0015124119140731216
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8880	0.0	29.958399	1
GTACATG	9435	0.0	24.92773	1
TATCAAC	10775	0.0	24.633951	2
TCAACGC	10660	0.0	24.59867	4
CAACGCA	10985	0.0	23.8709	5
ATCAACG	11160	0.0	23.578737	3
TACATGG	9775	0.0	23.402195	2
GGTATCA	6105	0.0	22.918789	1
AACGCAG	11680	0.0	22.522106	6
ACATGGG	10270	0.0	21.202934	3
ACGCAGA	13045	0.0	20.092268	7
CATGGGG	7700	0.0	19.8256	4
CGCAGAG	13655	0.0	19.258095	8
GTATAGC	655	0.0	18.972572	1
GAGTACA	7575	0.0	18.714224	1
GTACCTG	2920	0.0	18.441946	1
AGAGTAC	10370	0.0	18.289602	10-11
TAGGACG	235	3.5508874E-6	17.556887	4
GAGTACT	5425	0.0	17.527777	12-13
GTATAAG	980	0.0	17.377184	1
>>END_MODULE