FastQCFastQC Report
Mon 15 Aug 2016
SRR2921449_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921449_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3107619
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57880.18625191826926016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37680.1212503849410111No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35800.11520073728471864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95200.029.22211
TCAACGC113350.024.3072784
TATCAAC115700.023.8949152
CAACGCA115900.023.6767645
ATCAACG117350.023.4783613
GTACATG92150.023.1589151
GGTATCA66400.023.0288621
AACGCAG122200.022.4564746
TACATGG97400.021.1663842
ACGCAGA138800.019.7375077
ACATGGG103250.019.1393033
CGCAGAG144950.018.7362948
AGAGTAC108900.018.18040710-11
GAGTACA79100.017.7054251
GTACCTG30300.017.450821
AGTACTT61150.017.34986312-13
GAGTACT57050.017.22382712-13
CATGGGG77500.017.1014294
AGTACAT74650.016.9901542
GCAGAGT157050.016.8389959
Produced by FastQC (version 0.11.5)