##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921449_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3107619
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03010118035705	33.0	31.0	34.0	30.0	34.0
2	32.14636414566908	34.0	31.0	34.0	30.0	34.0
3	32.26892099707204	34.0	31.0	34.0	30.0	34.0
4	35.79451213292234	37.0	35.0	37.0	35.0	37.0
5	35.611378357514226	37.0	35.0	37.0	35.0	37.0
6	35.64641128787023	37.0	35.0	37.0	35.0	37.0
7	35.585215562139375	37.0	35.0	37.0	35.0	37.0
8	35.54038059363133	37.0	35.0	37.0	33.0	37.0
9	37.18337930100183	39.0	37.0	39.0	34.0	39.0
10-11	37.101217523769805	39.0	37.0	39.0	34.0	39.0
12-13	36.996088806253276	39.0	37.0	39.0	33.0	39.0
14-15	38.31994623536541	40.0	38.0	41.0	34.0	41.0
16-17	38.230109611249	40.0	38.0	41.0	33.5	41.0
18-19	38.13947848819305	40.0	38.0	41.0	33.5	41.0
20-21	38.03750266683271	40.0	38.0	41.0	33.0	41.0
22-23	38.004938668478985	40.0	38.0	41.0	33.5	41.0
24-25	38.05631111793305	40.0	38.0	41.0	33.0	41.0
26-27	37.956295961634936	40.0	38.0	41.0	33.0	41.0
28-29	37.834102571776015	40.0	38.0	41.0	33.0	41.0
30-31	37.699547627942806	40.0	38.0	41.0	33.0	41.0
32-33	37.53914154212598	40.0	38.0	41.0	32.5	41.0
34-35	37.40481796513666	40.0	37.0	41.0	32.0	41.0
36-37	37.319777134841814	40.0	37.0	41.0	32.0	41.0
38-39	37.29991997088446	40.0	37.0	41.0	32.0	41.0
40-41	37.148770650456186	40.0	37.0	41.0	31.5	41.0
42-43	36.924814303169086	40.0	37.0	41.0	31.0	41.0
44-45	36.73569330731985	40.0	36.0	41.0	31.0	41.0
46-47	36.47195746968982	39.0	36.0	41.0	30.0	41.0
48-49	36.15862417497125	39.0	35.0	40.5	30.0	41.0
50-51	35.90040075697825	39.0	35.0	40.0	29.0	41.0
52-53	35.61286438266724	38.5	35.0	40.0	28.0	41.0
54-55	35.30815811075939	38.0	35.0	40.0	28.0	41.0
56-57	34.94803674453014	38.0	34.0	40.0	27.0	41.0
58-59	34.58039917377259	37.5	34.0	40.0	26.5	41.0
60-61	34.17579230272437	37.0	33.5	39.5	26.0	41.0
62-63	33.76649148431645	36.0	33.0	39.0	26.0	40.5
64-65	33.27988292644626	36.0	33.0	39.0	24.5	40.0
66-67	32.77410181235216	35.0	32.0	38.0	23.0	40.0
68-69	32.29687358714179	35.0	31.5	37.5	22.0	40.0
70-71	31.7709744984826	35.0	31.0	37.0	20.5	39.0
72-73	31.2585281529042	34.5	31.0	36.0	19.5	39.0
74-75	30.76742982328271	34.0	30.0	36.0	18.0	38.5
76-77	29.402602281682533	32.5	28.5	34.5	18.0	36.0
78-79	30.357862080261448	34.0	30.0	35.0	18.0	37.0
80-81	30.413940061506896	34.0	31.0	35.0	18.0	36.5
82-83	30.113924197271288	34.0	30.0	35.0	16.0	36.0
84-85	29.675656668336757	34.0	30.0	35.0	9.0	36.0
86-87	29.21473723130152	34.0	29.0	35.0	6.0	35.0
88-89	28.714241353267568	33.5	29.0	35.0	2.0	35.0
90-91	28.302083363501126	33.0	28.5	35.0	2.0	35.0
92-93	27.92492322900587	33.0	27.0	35.0	2.0	35.0
94-95	27.50320212999084	33.0	27.0	35.0	2.0	35.0
96-97	26.82657140402347	33.0	25.5	34.0	2.0	35.0
98-99	25.915027710925955	32.0	24.5	34.0	2.0	35.0
100-101	22.31641539712558	28.5	10.0	32.5	2.0	34.5
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	18199.0
3	9645.0
4	5767.0
5	4202.0
6	3712.0
7	3929.0
8	4539.0
9	5274.0
10	6276.0
11	7292.0
12	8702.0
13	10319.0
14	11464.0
15	12931.0
16	14871.0
17	17774.0
18	17662.0
19	15102.0
20	14708.0
21	15366.0
22	16612.0
23	18700.0
24	21450.0
25	25320.0
26	30400.0
27	37099.0
28	45014.0
29	55664.0
30	68716.0
31	86738.0
32	113278.0
33	154080.0
34	224811.0
35	354109.0
36	569805.0
37	738382.0
38	329924.0
39	9783.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.53389026734253	17.96886954534273	14.262927603098325	24.234312584216415
2	16.217528596652294	19.463261101183896	38.06065029207248	26.258560010091326
3	19.87179652211299	22.669759065766794	28.363643736057494	29.09480067606272
4	14.355529467751923	15.77827713812215	34.70371578404064	35.16247761008529
5	15.825228190932217	35.51354021357352	32.79349464779033	15.86773694770393
6	34.602601132803315	33.418953686440815	16.72301703069002	15.25542815006585
7	30.67658582047495	30.543842372146674	19.676044155069594	19.103527652308777
8	27.780691265378486	32.834171301044734	19.399220960617665	19.98591647295911
9	26.856496833032445	16.372183508578107	19.425935797442914	37.34538386094653
10-11	26.3975732470946	24.958738114257738	26.261161001881106	22.382527636766564
12-13	26.721779900521103	22.221297065172788	26.68024446478716	24.37667856951895
14-15	23.973088725192106	23.805404441129934	24.479168427633404	27.742338406044553
16-17	24.169110231295505	26.680796366538235	25.12964117665805	24.02045222550821
18-19	24.09748413905353	26.07646476339601	26.537822900798748	23.28822819675171
20-21	24.79351811274131	25.143015231836362	26.446564166180764	23.61690248924157
22-23	24.668028419754755	24.772349007980583	26.33207137775288	24.227551194511783
24-25	24.476081612261606	25.001360032679415	26.324470641481547	24.19808771357744
26-27	24.326713928815813	25.21970269005441	26.39874589867927	24.054837482450502
28-29	24.34201304426963	25.364453069742122	26.275543499164677	24.017990386823573
30-31	24.586960963985693	25.119621305126444	26.062158991991996	24.23125873889587
32-33	24.45807227376415	25.16226737144638	25.991758190039192	24.387902164750276
34-35	24.697179511026853	25.13888759268693	26.036077466177982	24.127855430108237
36-37	24.6264994170163	25.079627238002494	26.07789228623904	24.215981058742166
38-39	24.548813378219197	25.217322038916816	26.051288323023748	24.18257625984024
40-41	24.75192411489749	25.119270501925804	25.91517962750719	24.21362575566951
42-43	24.68274153533612	25.152134908801116	25.750924008088116	24.414199547774647
44-45	24.6329744796697	25.08875328309103	25.76336191096254	24.51491032627673
46-47	24.74954166591565	25.090411547958997	25.800072998715116	24.359973787410233
48-49	24.74029296485323	25.177922086280468	25.603950391628103	24.4778345572382
50-51	24.602496631859193	25.38935353740467	25.59066159730568	24.41748823343046
52-53	24.804101885522872	25.319942919599107	25.420617197522226	24.45533799735579
54-55	24.718444867969367	25.164910575724836	25.525922219263446	24.59072233704235
56-57	24.76221813357747	25.26013890486714	25.506814512203853	24.470828449351533
58-59	24.852763527702855	25.203373293611183	25.490654160496284	24.453209018189682
60-61	24.836667245917887	25.183896172436654	25.644626157697925	24.334810423947534
62-63	24.666096590291023	25.274524923837838	25.526907626386063	24.532470859485077
64-65	24.709349755054742	25.236248358369572	25.502079049915544	24.552322836660146
66-67	24.750742011704794	25.226257235016515	25.405981805422318	24.617018947856376
68-69	24.650106892217643	25.22817330787261	25.468167627990933	24.653552171918815
70-71	24.725440590412553	25.09861610793592	25.535304357452937	24.640638944198585
72-73	24.871094925026068	25.113108298610737	25.402343008771677	24.613453767591515
74-75	24.641042572639034	25.387112529294026	25.305770202211264	24.666074695855677
76-77	24.749580906610323	25.159999502914165	25.347826936016176	24.742592654459337
78-79	24.705637042386304	25.135900389896975	25.487169950157917	24.67129261755881
80-81	24.725351838385944	25.23544145050668	25.446394801280235	24.59281190982714
82-83	24.783066950583667	25.153611790226943	25.41914424806548	24.64417701112391
84-85	24.782876320488054	25.273551938861473	25.26061358993319	24.682958150717283
86-87	24.709178980535683	25.209503573747092	25.409752616479025	24.671564829238196
88-89	24.612869499835078	25.13772503717019	25.355610049725797	24.893795413268936
90-91	24.722955986799327	25.151281884389064	25.34785166835402	24.77791046045759
92-93	24.793082716207373	25.243224492194074	25.347807925128755	24.6158848664698
94-95	24.76689260293123	25.159845991749926	25.31338439050952	24.759877014809327
96-97	24.701872427642527	25.111438319013118	25.425764935180496	24.760924318163863
98-99	24.678868081189716	25.192161014903665	25.2882576135475	24.840713290359123
100-101	25.127848163591587	24.711470726141528	25.397012354713606	24.76366875555328
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	713.0
1	569.5
2	668.0
3	890.5
4	723.5
5	654.5
6	1194.5
7	2573.0
8	3610.0
9	3324.5
10	2265.0
11	1354.5
12	1090.0
13	1041.5
14	1053.5
15	1113.0
16	1162.5
17	1279.0
18	1387.5
19	1413.5
20	1447.5
21	1549.0
22	1655.5
23	1797.5
24	1990.5
25	2279.0
26	2773.0
27	3518.0
28	4599.0
29	6146.0
30	8493.0
31	11413.0
32	15337.5
33	20003.0
34	24836.5
35	30442.0
36	37341.0
37	44820.0
38	52406.5
39	59651.0
40	67464.0
41	76427.5
42	85283.5
43	94041.5
44	102894.5
45	112057.0
46	120727.0
47	129765.0
48	140051.5
49	144587.5
50	142134.5
51	141149.5
52	141276.5
53	141391.0
54	139974.0
55	134709.0
56	126605.5
57	116194.0
58	106655.0
59	96474.0
60	83566.5
61	71030.0
62	58523.0
63	44453.5
64	32504.0
65	24554.0
66	18510.0
67	14083.0
68	10855.0
69	8129.0
70	6030.0
71	4466.0
72	3241.5
73	2371.5
74	1776.0
75	1311.0
76	916.5
77	556.0
78	315.0
79	168.0
80	82.0
81	36.0
82	20.0
83	16.0
84	8.5
85	5.0
86	2.5
87	1.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3200199252224935
2	0.0
3	0.006918480032462152
4	0.06011032884018279
5	7.722954454841471E-4
6	0.06593472365820906
7	0.07661814398740643
8	0.1056435811468523
9	0.09376953867253353
10-11	0.09843549032233359
12-13	0.09785626873822048
14-15	0.06694836143040701
16-17	0.037327613198400444
18-19	0.040127184188280485
20-21	0.02797962041035275
22-23	0.042765860293684656
24-25	0.034624579139205934
26-27	0.02230003098835475
28-29	0.02373199546018994
30-31	0.003346613597097971
32-33	0.005486515560626962
34-35	0.008044744223793199
36-37	0.010554704421616677
38-39	0.015413729932787772
40-41	0.019500459998474717
42-43	0.0031374502472793476
44-45	0.013193380527020846
46-47	0.023007968480048553
48-49	0.002542139174718651
50-51	0.006194453052320764
52-53	0.0023329758249000278
54-55	0.003266166154860039
56-57	0.0031052712703841753
58-59	0.0010619062375407024
60-61	0.0051164573263324745
62-63	0.013933496995609824
64-65	0.006114005610082832
66-67	0.03858259329731219
68-69	0.061799725127179364
70-71	0.01184186349742359
72-73	0.031294055030555545
74-75	0.05180815280122821
76-77	0.30771146656008985
78-79	0.07438170509319193
80-81	0.03243640871033418
82-83	0.027110788034183084
84-85	0.018551180180067118
86-87	0.12015629972657524
88-89	0.539593174066705
90-91	0.13325314332291058
92-93	0.04783404915467437
94-95	0.008141281154478718
96-97	0.005872663283369036
98-99	0.01058688339851185
100-101	0.007143732870728362
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3107619.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.86040102044223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.54848558682743	40.52587498305954
2	15.414978892356443	17.221738052973663
3	5.376082382724112	9.009303534537105
4	2.237498501911955	4.999502543977622
5	1.2287736861949052	3.431989543710722
6	0.7071049025902786	2.3699498053328223
7	0.4767320714636294	1.8641311283885125
8	0.3430952911540926	1.5332352441674397
9	0.2546402467460256	1.2801875669259615
>10	1.331852697957871	13.195451009217562
>50	0.06457142144591417	2.421578547433734
>100	0.015146443172611436	1.3222508350945241
>500	6.342572228916452E-4	0.22626798748193888
>1k	3.459584845143432E-4	0.41211372170576255
>5k	5.76597474190572E-5	0.18642549599319685
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5788	0.18625191826926016	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3768	0.1212503849410111	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3580	0.11520073728471864	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.287159075806912E-4	0.0	0.0	0.0
6	0.0	1.287159075806912E-4	0.0	0.0	0.0
7	0.0	1.60894884475864E-4	0.0	6.43579537903456E-5	0.0
8	0.0	1.60894884475864E-4	0.0	6.43579537903456E-5	0.0
9	0.0	1.60894884475864E-4	0.0	6.43579537903456E-5	0.0
10-11	0.0	1.60894884475864E-4	0.0	9.65369306855184E-5	0.0
12-13	0.0	1.60894884475864E-4	0.0	1.287159075806912E-4	1.60894884475864E-4
14-15	0.0	2.0916334981862318E-4	0.0	1.287159075806912E-4	1.930738613710368E-4
16-17	0.0	2.252528382662096E-4	0.0	1.769843729234504E-4	2.252528382662096E-4
18-19	0.0	2.252528382662096E-4	0.0	2.252528382662096E-4	3.057002805041416E-4
20-21	0.0	2.574318151613824E-4	0.0	2.896107920565552E-4	3.378792573993144E-4
22-23	0.0	2.574318151613824E-4	0.0	3.8614772274207364E-4	3.7005823429448717E-4
24-25	0.0	2.735213036089688E-4	0.0	5.631320956655239E-4	3.861477227420736E-4
26-27	0.0	2.896107920565552E-4	0.0	8.366533992744927E-4	3.861477227420736E-4
28-29	1.60894884475864E-5	2.896107920565552E-4	0.0	0.0017537542407869175	3.861477227420736E-4
30-31	3.21789768951728E-5	3.21789768951728E-4	0.0	0.005100367837884889	4.0223721118966E-4
32-33	3.21789768951728E-5	3.21789768951728E-4	0.0	0.012356727127746355	4.183266996372464E-4
34-35	3.21789768951728E-5	3.21789768951728E-4	0.0	0.022444836384383028	5.470426072179376E-4
36-37	3.21789768951728E-5	3.21789768951728E-4	0.0	0.03571866435364181	5.470426072179376E-4
38-39	3.21789768951728E-5	3.21789768951728E-4	0.0	0.055991419797600674	5.470426072179376E-4
40-41	3.21789768951728E-5	3.21789768951728E-4	0.0	0.07850061413577404	5.470426072179376E-4
42-43	3.21789768951728E-5	3.539687458469008E-4	0.0	0.10189473033856467	5.470426072179376E-4
44-45	3.21789768951728E-5	3.539687458469008E-4	0.0	0.12680125845542842	5.470426072179376E-4
46-47	3.21789768951728E-5	3.539687458469008E-4	0.0	0.15407294137408736	5.792215841131104E-4
48-49	3.21789768951728E-5	3.539687458469008E-4	0.0	0.18005746521693938	6.114005610082832E-4
50-51	3.21789768951728E-5	3.539687458469008E-4	0.0	0.20658903166700937	6.43579537903456E-4
52-53	3.21789768951728E-5	3.7005823429448717E-4	0.0	0.23160818620300622	6.596690263510423E-4
54-55	3.21789768951728E-5	4.6659516498000556E-4	0.0	0.2592338378675121	6.757585147986288E-4
56-57	3.21789768951728E-5	5.148636303227647E-4	0.0	0.28729390572010277	6.757585147986288E-4
58-59	3.21789768951728E-5	5.470426072179376E-4	0.0	0.31709163832503273	6.757585147986288E-4
60-61	3.21789768951728E-5	5.470426072179376E-4	0.0	0.34600444906534555	6.918480032462153E-4
62-63	3.21789768951728E-5	5.470426072179376E-4	0.0	0.3761078819507797	7.722954454841472E-4
64-65	3.21789768951728E-5	5.470426072179376E-4	0.0	0.4067905364203269	7.722954454841472E-4
66-67	3.21789768951728E-5	6.114005610082832E-4	0.0	0.43892124485015693	7.883849339317336E-4
68-69	3.21789768951728E-5	6.114005610082832E-4	0.0	0.4723712913326891	8.0447442237932E-4
70-71	3.21789768951728E-5	6.596690263510424E-4	0.0	0.5082347610823592	8.0447442237932E-4
72-73	3.21789768951728E-5	7.079374916938016E-4	0.0	0.5420709552876334	0.0010297272606455295
74-75	3.21789768951728E-5	7.079374916938016E-4	0.0	0.5780631409448842	0.0011262641913310479
76-77	3.21789768951728E-5	7.079374916938016E-4	0.0	0.6158895282851597	0.0011745326566738072
78-79	3.21789768951728E-5	7.079374916938016E-4	0.0	0.6562097863348113	0.0012228011220165665
80-81	3.21789768951728E-5	7.401164685889743E-4	0.0	0.6952267958202083	0.0012549800989117392
82-83	3.21789768951728E-5	7.401164685889743E-4	0.0	0.7365285126651626	0.001287159075806912
84-85	3.21789768951728E-5	7.401164685889743E-4	0.0	0.7781681087675162	0.0013032485642544985
86-87	3.21789768951728E-5	7.401164685889743E-4	0.0	0.8216901750182375	0.0013836960064924305
88-89	3.21789768951728E-5	7.401164685889743E-4	0.0	0.8678509173743628	0.0014802329371779487
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	9520	0.0	29.2221	1
TCAACGC	11335	0.0	24.307278	4
TATCAAC	11570	0.0	23.894915	2
CAACGCA	11590	0.0	23.676764	5
ATCAACG	11735	0.0	23.478361	3
GTACATG	9215	0.0	23.158915	1
GGTATCA	6640	0.0	23.028862	1
AACGCAG	12220	0.0	22.456474	6
TACATGG	9740	0.0	21.166384	2
ACGCAGA	13880	0.0	19.737507	7
ACATGGG	10325	0.0	19.139303	3
CGCAGAG	14495	0.0	18.736294	8
AGAGTAC	10890	0.0	18.180407	10-11
GAGTACA	7910	0.0	17.705425	1
GTACCTG	3030	0.0	17.45082	1
AGTACTT	6115	0.0	17.349863	12-13
GAGTACT	5705	0.0	17.223827	12-13
CATGGGG	7750	0.0	17.101429	4
AGTACAT	7465	0.0	16.990154	2
GCAGAGT	15705	0.0	16.838995	9
>>END_MODULE