FastQCFastQC Report
Mon 15 Aug 2016
SRR2921448_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921448_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6466622
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA133950.030.2390541
GTACCTG76200.028.6319941
TACCTGG77900.026.960422
GTACATG209550.025.7874661
TATCAAC163850.024.3484572
TCAACGC163850.024.3202824
TACATGG217650.024.2291282
ATCAACG167050.023.9643883
CAACGCA165600.023.8417475
AACGCAG176550.022.4878546
ACATGGG226450.022.3965643
GAGTACA156800.021.8401261
AGTACAT151400.020.7170942
GGTATCA95250.020.1511271
CATGGGG182250.019.3991224
ACCTGGG105500.019.3422153
ACGCAGA205500.019.115677
AGAACCG30750.018.0441115
CGCAGAG218700.017.916538
GTATAGG16200.017.6159041
Produced by FastQC (version 0.11.5)