##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921448_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6466622 Sequences flagged as poor quality 0 Sequence length 98 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.106414137087338 31.0 30.0 34.0 24.0 34.0 2 29.170781437356318 31.0 30.0 34.0 23.0 34.0 3 29.293184447768866 31.0 30.0 34.0 23.0 34.0 4 32.74245904585114 35.0 35.0 37.0 26.0 37.0 5 32.671039686562786 35.0 35.0 37.0 25.0 37.0 6 32.76840628693002 37.0 35.0 37.0 25.0 37.0 7 32.614376717859805 36.0 35.0 37.0 25.0 37.0 8 32.568973259918394 36.0 35.0 37.0 25.0 37.0 9 33.86652923272769 38.0 35.0 39.0 23.0 39.0 10-11 33.68678956339183 38.0 34.0 39.0 23.0 39.0 12-13 33.472977545308815 38.0 34.0 39.0 20.0 39.0 14-15 34.28191434415062 38.0 34.0 40.0 18.0 41.0 16-17 34.07797417260511 38.0 33.0 40.0 17.5 41.0 18-19 33.847034201164064 38.0 33.0 40.0 17.5 41.0 20-21 33.69553841248182 38.0 33.0 40.0 16.0 41.0 22-23 33.70432831855643 38.0 33.0 40.0 13.5 41.0 24-25 33.51122030945987 38.0 33.0 40.0 10.0 41.0 26-27 33.163200586024665 38.0 32.5 40.0 9.5 41.0 28-29 32.86204018110228 38.0 32.0 40.0 8.5 41.0 30-31 32.546320087984114 38.0 31.5 40.0 8.0 41.0 32-33 32.21430648026126 38.0 31.0 40.0 4.0 41.0 34-35 31.73837569290427 37.0 30.0 40.0 2.0 41.0 36-37 31.297050685814014 37.0 30.0 40.0 2.0 41.0 38-39 30.996168787969978 37.0 30.0 39.5 2.0 41.0 40-41 30.667793633213755 36.0 29.0 39.0 2.0 40.0 42-43 30.321570056205545 36.0 28.0 39.0 2.0 40.0 44-45 29.949044338759865 35.5 27.0 39.0 2.0 40.0 46-47 29.607049167246824 35.0 26.5 39.0 2.0 40.0 48-49 29.324738170871903 35.0 26.0 39.0 2.0 40.0 50-51 28.6899773173691 34.0 25.5 38.0 2.0 39.5 52-53 29.340371758237918 35.0 27.5 38.5 2.0 39.5 54-55 29.721132377924672 35.5 28.0 39.0 2.0 40.0 56-57 29.51688145681006 35.0 27.5 39.0 2.0 40.0 58-59 29.1217424646129 35.0 26.5 39.0 2.0 40.0 60-61 28.602452254051652 34.5 26.0 38.0 2.0 40.0 62-63 28.053317790957937 34.0 24.5 38.0 2.0 40.0 64-65 27.435852907437607 34.0 22.5 37.0 2.0 39.5 66-67 26.885049489517094 33.0 21.5 36.5 2.0 39.0 68-69 26.31143045008661 33.0 19.5 36.0 2.0 39.0 70-71 25.73117943185793 32.0 17.5 35.5 2.0 38.5 72-73 25.12142429231212 32.0 11.5 35.0 2.0 37.5 74-75 24.46448369798018 31.0 7.0 35.0 2.0 37.0 76-77 23.81651857801492 31.0 2.0 34.5 2.0 36.0 78-79 23.181559707680456 30.0 2.0 34.0 2.0 36.0 80-81 22.531631197864975 30.0 2.0 34.0 2.0 35.0 82-83 21.827772599047847 29.0 2.0 34.0 2.0 35.0 84-85 21.131240623002242 29.0 2.0 34.0 2.0 35.0 86-87 20.43111171489535 27.5 2.0 33.5 2.0 35.0 88-89 19.693159117697 26.5 2.0 33.0 2.0 35.0 90-91 18.87026936165435 25.0 2.0 33.0 2.0 34.0 92-93 17.98542059517318 24.5 2.0 32.0 2.0 34.0 94-95 17.023783746753715 19.5 2.0 32.0 2.0 34.0 96-97 16.11911419903622 9.5 2.0 31.5 2.0 34.0 98 15.507290359634442 2.0 2.0 31.0 2.0 34.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 365712.0 3 58535.0 4 43347.0 5 36162.0 6 34331.0 7 36175.0 8 41212.0 9 46515.0 10 51553.0 11 55569.0 12 57967.0 13 59278.0 14 54032.0 15 55223.0 16 59765.0 17 67549.0 18 74879.0 19 77660.0 20 83633.0 21 89260.0 22 97245.0 23 106075.0 24 116642.0 25 128002.0 26 141994.0 27 158853.0 28 178081.0 29 202260.0 30 232696.0 31 273147.0 32 325790.0 33 399801.0 34 498592.0 35 627113.0 36 727336.0 37 605206.0 38 193646.0 39 5786.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.654217998464915 16.486717020981448 12.898975976353366 24.960089004200267 2 16.413808168722362 18.108377298522576 38.54020822961509 26.937606303139965 3 21.013281223407382 21.360041217589885 26.652014618010107 30.974662940992626 4 14.039780797163113 13.93318088566115 33.597367956902076 38.42967036027367 5 16.2029576901212 35.12752938147345 32.120402070574954 16.549110857830403 6 36.77729084392778 31.843642758974553 16.04151663819027 15.337549758907398 7 32.81557179994443 29.240921008582244 18.22187472733787 19.721632464135457 8 28.895847891403676 33.17781524853661 17.98362876356085 19.94270809649886 9 27.279930045790685 16.351533511580058 18.134877134723094 38.23365930790616 10-11 27.428284335358942 24.382889063362594 25.317267661383298 22.871558939895166 12-13 27.412203543856357 21.312774638196338 26.129967072414246 25.145054745533063 14-15 24.429270972472075 22.917039625837308 23.512926239650554 29.140763162040066 16-17 25.120673426543792 26.051471140917176 24.037218607819174 24.790636824719854 18-19 25.166927938002882 25.38873523424992 25.349258300909078 24.095078526838126 20-21 25.625666981016515 24.5240006379339 25.301840467155806 24.54849191389378 22-23 25.330941262859664 24.21869969438955 25.190418344552256 25.259940698198534 24-25 25.095597689918176 24.546843777169315 25.303075799530777 25.054482733381732 26-27 25.17919496678583 24.645324432885886 25.29550880475092 24.879971795577365 28-29 25.13882409094296 24.776460306242164 25.214149204238232 24.87056639857664 30-31 25.275659482384484 24.67910706519141 25.06257410294722 24.982659349476886 32-33 25.17923558736741 24.72939865818488 25.02677628391585 25.064589470531857 34-35 25.25121475408627 24.708204833869452 25.195876064619753 24.84470434742452 36-37 25.36386807161123 24.659334546123198 25.082613142124472 24.894184240141104 38-39 25.325433887230602 24.654150451142584 25.1049509612076 24.915464700419214 40-41 25.377123581914113 24.653685021483618 25.088037352807874 24.881154043794396 42-43 25.317575164734162 24.506081191832614 25.083901087144824 25.092442556288397 44-45 25.289362653819953 24.60076400785984 25.02696128066355 25.082912057656653 46-47 25.401767835977495 24.67507905750432 25.03941414228377 24.883738964234414 48-49 25.36532412775475 24.660151676990104 24.90244763513986 25.072076560115285 50-51 25.115585655225747 24.8597275799573 24.94904906613007 25.075637698686887 52-53 25.21221527582931 24.885645291836635 24.943137597681705 24.959001834652348 54-55 25.109714914589127 24.59667281407075 25.1150014436889 25.17861082765122 56-57 25.143823958697443 24.759301289492626 25.101416970038144 24.995457781771787 58-59 25.27759092241905 24.682373083256376 25.052774013477514 24.98726198084705 60-61 25.2779657142271 24.576785549302866 25.10846158490479 25.036787151565242 62-63 25.066886109006152 24.774760877351774 25.118262536419483 25.040090477222588 64-65 25.13219351814348 24.680051228801798 25.111059344433322 25.0766959086214 66-67 25.146744061470095 24.794821099672763 24.967248967710788 25.091185871146358 68-69 25.102520398196226 24.677665641093547 25.062669379457937 25.15714458125229 70-71 25.040145454161323 24.531843496964605 25.177603924180282 25.25040712469379 72-73 25.21730999703647 24.54697443473928 25.000574688720633 25.23514087950362 74-75 24.969564698513288 24.77103157172874 25.047575661977028 25.211828067780946 76-77 25.165881101750426 24.55291914473278 25.01931134579909 25.261888407717702 78-79 25.11937351067029 24.47871567451013 25.14489794381161 25.257012871007962 80-81 25.112668375538625 24.618378803817063 25.035647226520375 25.233305594123934 82-83 25.216480803252413 24.583217904087597 24.995213909323134 25.205087383336856 84-85 25.195578021348712 24.631409905539012 24.960011726684403 25.213000346427872 86-87 25.168163885241633 24.548677907898796 25.122875032347796 25.160283174511775 88-89 25.136923900498537 24.361973804147198 25.0833837294884 25.417718565865865 90-91 25.15594977460225 24.38958577363784 25.006831234914113 25.4476332168458 92-93 25.232372629191214 24.547412138109266 25.069347435680328 25.150867797019195 94-95 25.30456208304763 24.34479218678534 25.03437345420406 25.316272275962966 96-97 25.244325195009786 24.364793317890335 25.08810926387339 25.302772223226494 98 25.31978344949634 24.440850660469096 24.911879995912738 25.327485894121825 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 658.0 1 485.0 2 433.0 3 600.5 4 554.0 5 518.0 6 838.5 7 1611.5 8 2313.0 9 2317.0 10 2016.0 11 1911.5 12 2114.5 13 2563.0 14 3004.5 15 3470.0 16 3854.5 17 4101.5 18 4138.5 19 3946.5 20 3672.5 21 3319.0 22 3067.0 23 2989.5 24 3053.5 25 3317.5 26 3854.5 27 4740.5 28 6116.0 29 8357.5 30 11909.0 31 16885.5 32 23851.5 33 32163.0 34 42414.0 35 55315.5 36 70039.5 37 86252.0 38 103394.0 39 120767.5 40 138914.0 41 159098.0 42 180602.5 43 202970.5 44 224813.0 45 245497.5 46 268915.0 47 292061.0 48 304413.0 49 307889.0 50 309047.0 51 309498.5 52 312006.0 53 315100.0 54 313539.0 55 303571.0 56 286197.0 57 268068.0 58 248958.5 59 226144.0 60 198866.0 61 167037.0 62 134661.0 63 103896.5 64 77884.0 65 58762.5 66 44406.5 67 33278.5 68 24834.0 69 18595.0 70 13794.0 71 10232.5 72 7786.5 73 5579.5 74 4088.0 75 3022.0 76 1980.0 77 1188.5 78 702.0 79 399.5 80 244.5 81 153.0 82 85.5 83 58.0 84 45.5 85 30.5 86 24.5 87 21.5 88 16.5 89 14.5 90 12.0 91 10.5 92 12.5 93 11.0 94 11.0 95 7.5 96 8.0 97 6.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.45113198204564914 2 0.04323741205222758 3 0.05199004982817922 4 0.04951580593391728 5 0.05993855833849574 6 0.061237536382983265 7 0.03986625474629567 8 0.017567131649259844 9 0.010484608501935014 10-11 0.012796480140636024 12-13 0.00951037496856937 14-15 0.013585145381932019 16-17 0.012742356055449043 18-19 0.051866337633466125 20-21 0.0794464250423173 22-23 0.09448518871212822 24-25 0.06577622752652003 26-27 0.08078406314765267 28-29 0.08192066893657925 30-31 0.0538921248218931 32-33 0.03009299136396097 34-35 0.37847426368821313 36-37 0.5948700882779293 38-39 0.5456480988064557 40-41 0.5408851793100015 42-43 0.5149906705541162 44-45 0.5009570684663492 46-47 0.4909673087432666 48-49 0.4809930130445231 50-51 0.49484877885239004 52-53 0.573189526154459 54-55 0.5440398402751855 56-57 0.5032148160198633 58-59 0.4819053904805322 60-61 0.49439259013438547 62-63 0.5062534968025036 64-65 0.5244237254009899 66-67 0.5493903926965269 68-69 0.5050318388797118 70-71 0.45471963569232904 72-73 0.4384669461118958 74-75 0.4197322806250311 76-77 0.4337581507006285 78-79 0.42825295803589575 80-81 0.4249745848759986 82-83 0.44374791042371114 84-85 0.4114822236401014 86-87 0.41517039344498563 88-89 0.4401911848257096 90-91 0.4244797360971463 92-93 0.4194848562356049 94-95 0.42969884431160504 96-97 0.4246111803040289 98 0.41919877178533094 >>END_MODULE >>Sequence Length Distribution pass #Length Count 98 6466622.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.850055359745475 #Duplication Level Percentage of deduplicated Percentage of total 1 76.11324341150652 40.225891279079015 2 12.282591037561273 12.982712325924538 3 4.530569392869088 7.183225296728993 4 2.1852302178746075 4.6195815195384675 5 1.2318596865600733 3.25519263150693 6 0.7633856930070482 2.42069856817561 7 0.5203627591076917 1.9250840438193986 8 0.3810537205330677 1.6110968180167689 9 0.2875185715174931 1.3675835179489007 >10 1.5417680001554037 14.838233002514114 >50 0.11171346286388112 4.046789024462474 >100 0.04869794197795775 4.477372947691311 >500 0.0015045936047315823 0.5462984538752307 >1k 5.015108612049702E-4 0.500240570718168 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.639207301741156E-5 2 0.0 0.0 0.0 0.0 4.639207301741156E-5 3 0.0 0.0 0.0 0.0 4.639207301741156E-5 4 0.0 0.0 0.0 1.5464024339137188E-5 4.639207301741156E-5 5 0.0 0.0 0.0 1.5464024339137188E-5 4.639207301741156E-5 6 0.0 0.0 0.0 1.5464024339137188E-5 6.185609735654875E-5 7 0.0 0.0 0.0 1.5464024339137188E-5 6.185609735654875E-5 8 0.0 0.0 0.0 4.639207301741156E-5 7.732012169568594E-5 9 0.0 0.0 0.0 6.185609735654875E-5 7.732012169568594E-5 10-11 0.0 0.0 0.0 8.505213386525453E-5 7.732012169568594E-5 12-13 0.0 0.0 0.0 1.237121947130975E-4 2.0876432857835204E-4 14-15 0.0 7.732012169568594E-6 0.0 1.3917621905223468E-4 2.86084450274038E-4 16-17 0.0 1.5464024339137188E-5 0.0 2.1649634074792065E-4 3.711365841392925E-4 18-19 0.0 1.5464024339137188E-5 0.0 3.556725598001553E-4 4.252606693262727E-4 20-21 0.0 2.319603650870578E-5 0.0 3.9433262064799833E-4 4.4845670583497843E-4 22-23 0.0 3.0928048678274376E-5 0.0 4.3299268149584125E-4 4.716527423436842E-4 24-25 0.0 3.0928048678274376E-5 0.0 4.716527423436843E-4 5.257768275306644E-4 26-27 0.0 3.0928048678274376E-5 0.0 5.799009127176446E-4 5.412408518698016E-4 28-29 0.0 3.866006084784297E-5 0.0 0.0010438216428917601 5.412408518698016E-4 30-31 0.0 4.639207301741156E-5 0.0 0.00252836797944893 5.412408518698016E-4 32-33 0.0 4.639207301741156E-5 0.0 0.005203644190119663 6.030969492263503E-4 34-35 0.0 4.639207301741156E-5 0.0 0.008953670092360432 6.340249979046247E-4 36-37 0.0 5.4124085186980156E-5 0.0 0.014590306963975936 6.340249979046247E-4 38-39 0.0 6.958810952611735E-5 0.0 0.023041396265314408 6.340249979046247E-4 40-41 0.0 8.505213386525453E-5 0.0 0.0321033145280488 6.340249979046247E-4 42-43 0.0 1.0824817037396031E-4 0.0 0.04107244864474837 6.417570100741933E-4 44-45 0.0 1.0824817037396031E-4 0.0 0.05113179647735711 6.64953046582899E-4 46-47 0.0 1.1598018254352891E-4 0.0 0.06146176473590075 7.036131074307421E-4 48-49 0.0 1.314442068826661E-4 0.0 0.07240256195584031 7.654692047872908E-4 50-51 0.0 1.5464024339137189E-4 0.0 0.08436398478216292 8.659853629916825E-4 52-53 7.732012169568594E-6 1.7010426773050906E-4 0.0 0.09545169023332428 9.355734725177999E-4 54-55 1.5464024339137188E-5 1.7010426773050906E-4 0.0 0.10691053226862496 9.433054846873684E-4 56-57 1.5464024339137188E-5 1.7783627990007765E-4 0.0 0.11959876423888702 9.433054846873684E-4 58-59 1.5464024339137188E-5 2.0103231640878345E-4 0.0 0.13295968126790153 9.510374968569371E-4 60-61 1.5464024339137188E-5 2.2422835291748921E-4 0.0 0.14649843457681616 9.974295698743486E-4 62-63 3.0928048678274376E-5 2.3196036508705783E-4 0.0 0.15992120770318724 0.001067017679400466 64-65 3.866006084784297E-5 2.3196036508705783E-4 0.0 0.17303470034277557 0.0010824817037396032 66-67 4.639207301741156E-5 2.47424389426195E-4 0.0 0.18607860487283778 0.0010824817037396032 68-69 4.639207301741156E-5 2.47424389426195E-4 0.0 0.20080808805586595 0.0010979457280787404 70-71 6.185609735654875E-5 2.5515640159576357E-4 0.0 0.21617932824896832 0.001105677740248309 72-73 7.732012169568594E-5 2.628884137653322E-4 0.0 0.2312490199674575 0.0012680499958092493 74-75 7.732012169568594E-5 2.628884137653322E-4 0.0 0.24614087540604662 0.001353102129674504 76-77 8.505213386525453E-5 2.628884137653322E-4 0.0 0.26152757962348816 0.0013762981661832097 78-79 9.278414603482312E-5 2.628884137653322E-4 0.0 0.27849161432352165 0.0014072262148614842 80-81 9.278414603482312E-5 2.628884137653322E-4 7.732012169568594E-6 0.2957030734129813 0.0014226902392006212 82-83 9.278414603482312E-5 2.628884137653322E-4 1.5464024339137188E-5 0.31327793707441076 0.0015309384095745816 84-85 9.278414603482312E-5 2.706204259349008E-4 1.5464024339137188E-5 0.32995588732417014 0.0015386704217441502 86 9.278414603482312E-5 2.7835243810446936E-4 1.5464024339137188E-5 0.3431157720367759 0.0015464024339137189 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13395 0.0 30.239054 1 GTACCTG 7620 0.0 28.631994 1 TACCTGG 7790 0.0 26.96042 2 GTACATG 20955 0.0 25.787466 1 TATCAAC 16385 0.0 24.348457 2 TCAACGC 16385 0.0 24.320282 4 TACATGG 21765 0.0 24.229128 2 ATCAACG 16705 0.0 23.964388 3 CAACGCA 16560 0.0 23.841747 5 AACGCAG 17655 0.0 22.487854 6 ACATGGG 22645 0.0 22.396564 3 GAGTACA 15680 0.0 21.840126 1 AGTACAT 15140 0.0 20.717094 2 GGTATCA 9525 0.0 20.151127 1 CATGGGG 18225 0.0 19.399122 4 ACCTGGG 10550 0.0 19.342215 3 ACGCAGA 20550 0.0 19.11567 7 AGAACCG 3075 0.0 18.044111 5 CGCAGAG 21870 0.0 17.91653 8 GTATAGG 1620 0.0 17.615904 1 >>END_MODULE