FastQCFastQC Report
Mon 15 Aug 2016
SRR2921448_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921448_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6466622
Sequences flagged as poor quality0
Sequence length101
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG72700.031.4478451
TACCTGG77750.028.4682312
GTATCAA137900.027.9081571
GTACATG217250.025.0593931
TACATGG225200.023.5591562
TCAACGC164350.023.0351184
CAACGCA169200.022.3925025
ACATGGG225800.022.3194353
ATCAACG173300.021.8723833
TATCAAC174400.021.869762
AACGCAG180100.021.1168446
ACCTGGG104450.020.828063
GGTATCA98800.020.6816141
GAGTACA166400.020.3803081
AGTACAT158150.020.0323372
CATGGGG180850.019.0949174
GTATAGG15000.018.0995461
ACGCAGA210750.018.0018147
AGAGTAC166850.017.77132410-11
GTACTAG9200.017.084811
Produced by FastQC (version 0.11.5)