FastQCFastQC Report
Mon 15 Aug 2016
SRR2921447_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921447_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2695280
Sequences flagged as poor quality0
Sequence length98
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40150.14896411504556115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49000.037.958541
GGTATCA28700.035.1306721
TCAACGC57950.031.6490554
TATCAAC59750.030.619442
CAACGCA60450.030.3401625
ATCAACG61450.029.6977423
AACGCAG63100.029.4183676
ACGCAGA74450.024.8622427
GTACATG72600.023.9706291
CGCAGAG78550.023.4417868
GTACCTG24800.022.8337751
TACATGG75000.022.1925722
ACATGGG76500.020.8583563
TACCTGG26600.020.6853032
GCAGAGT89900.020.176529
GAGTACA57100.020.1571041
AGTACAT54550.018.9125482
GTATATA6650.018.6924551
CATGGGG60000.018.111374
AGAGTAC73800.017.96830210-11
Produced by FastQC (version 0.11.5)