##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921447_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2695280
Sequences flagged as poor quality	0
Sequence length	98
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.43415897420676	31.0	31.0	34.0	27.0	34.0
2	30.538716200172153	31.0	31.0	34.0	27.0	34.0
3	30.669500014840757	31.0	31.0	34.0	27.0	34.0
4	34.19872369475527	37.0	35.0	37.0	30.0	37.0
5	34.18935101362381	37.0	35.0	37.0	30.0	37.0
6	34.29128031224956	37.0	35.0	37.0	31.0	37.0
7	34.18189650054911	37.0	35.0	37.0	30.0	37.0
8	34.1655037695527	37.0	35.0	37.0	30.0	37.0
9	35.62776817250898	39.0	37.0	39.0	30.0	39.0
10-11	35.504583011783566	39.0	35.0	39.0	30.0	39.0
12-13	35.35045097355377	39.0	35.0	39.0	30.0	39.0
14-15	36.36964044551958	40.0	36.0	41.0	30.0	41.0
16-17	36.22909660591849	39.5	36.0	41.0	29.0	41.0
18-19	36.05926972336826	39.0	36.0	41.0	28.5	41.0
20-21	35.953180374580754	39.0	36.0	40.5	27.5	41.0
22-23	35.990656814876374	39.0	36.0	41.0	27.5	41.0
24-25	35.86087809058799	39.0	36.0	41.0	27.0	41.0
26-27	35.60207770621234	39.0	36.0	40.5	27.0	41.0
28-29	35.37658907423348	39.0	35.0	40.0	26.5	41.0
30-31	35.134187171648215	39.0	35.0	40.0	25.5	41.0
32-33	34.86661385830044	38.0	34.5	40.0	24.5	41.0
34-35	34.45467762162002	38.0	34.0	40.0	23.5	41.0
36-37	34.082719791635746	38.0	33.5	40.0	21.0	41.0
38-39	33.86025385117687	38.0	33.0	40.0	19.0	41.0
40-41	33.5981808940073	38.0	33.0	40.0	18.0	41.0
42-43	33.32629615475943	38.0	33.0	40.0	15.5	41.0
44-45	33.01460312101155	38.0	32.0	40.0	15.0	41.0
46-47	32.732399045739214	37.0	32.0	40.0	12.5	41.0
48-49	32.519266458401354	37.0	31.0	40.0	9.0	41.0
50-51	31.78014992876436	36.0	30.5	39.0	9.0	40.0
52-53	32.315154826214716	37.0	31.5	39.0	9.0	40.0
54-55	32.76636008132736	37.0	32.0	40.0	8.0	40.5
56-57	32.62685157015227	37.0	32.0	40.0	7.5	41.0
58-59	32.30758065952331	36.5	32.0	39.5	4.5	41.0
60-61	31.86158451070019	36.0	31.0	39.0	2.0	40.5
62-63	31.38438752189012	35.5	31.0	39.0	2.0	40.0
64-65	30.84947278204862	35.0	30.0	38.5	2.0	40.0
66-67	30.369331015701523	35.0	29.5	38.0	2.0	40.0
68-69	29.857421677896173	34.5	29.0	37.0	2.0	39.5
70-71	29.33916939983972	34.0	28.5	36.5	2.0	39.0
72-73	28.790898533733042	34.0	28.0	36.0	2.0	39.0
74-75	28.19661148377905	34.0	26.5	35.5	2.0	38.0
76-77	27.613030186103114	33.0	26.0	35.0	2.0	37.0
78-79	27.05019738209017	33.0	25.5	35.0	2.0	36.5
80-81	26.480270138909503	32.0	24.5	35.0	2.0	36.0
82-83	25.85307333560892	32.0	22.5	34.5	2.0	35.5
84-85	25.235577750734617	31.5	20.0	34.0	2.0	35.0
86-87	24.61618180671396	31.0	18.0	34.0	2.0	35.0
88-89	23.965841953340657	31.0	11.0	34.0	2.0	35.0
90-91	23.22979838829361	30.0	4.5	34.0	2.0	35.0
92-93	22.410165363153364	29.5	2.0	34.0	2.0	35.0
94-95	21.495513638657208	29.0	2.0	34.0	2.0	35.0
96-97	20.583506908373153	29.0	2.0	33.5	2.0	34.0
98	19.949804101985695	27.0	2.0	33.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	76733.0
3	13549.0
4	10113.0
5	8893.0
6	8326.0
7	8624.0
8	9738.0
9	11464.0
10	12344.0
11	14092.0
12	15627.0
13	17979.0
14	14957.0
15	15006.0
16	17072.0
17	19474.0
18	22193.0
19	21543.0
20	22403.0
21	24302.0
22	27152.0
23	29956.0
24	33226.0
25	37982.0
26	43086.0
27	49650.0
28	57802.0
29	67883.0
30	81527.0
31	99593.0
32	124578.0
33	161901.0
34	218826.0
35	304530.0
36	404671.0
37	414171.0
38	167787.0
39	6527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.349937480008144	17.339362393013268	13.408804693808621	24.90189543316997
2	16.125495087176176	18.881687620871798	38.60681447826071	26.386002813691317
3	19.867075915147762	22.028252400585362	28.224892137929807	29.87977954633707
4	13.944379583450115	14.88394625426211	34.7010444373087	36.47062972497907
5	15.549613681227518	35.58795782886288	33.053598272285775	15.808830217623829
6	35.22285334104586	33.27795213059222	16.433143626415607	15.066050901946312
7	31.05663837037092	30.534833395815316	19.321816852318577	19.08671138149519
8	27.76682852429998	33.75902447532336	19.03657289809278	19.43757410228388
9	26.87791123319959	16.26216303165663	19.034311966571536	37.82561376857224
10-11	26.462390788475204	24.978579790974383	26.30446197147559	22.254567449074823
12-13	26.562408394292486	22.099322665079516	26.8040433785261	24.534225562101895
14-15	23.74895077959581	23.78754279446264	24.273932058757826	28.189574367183724
16-17	24.133866492707092	26.868942909867194	24.98360779834642	24.01358279907929
18-19	24.154137623620684	26.115400528143688	26.430274694485377	23.300187153750247
20-21	24.610748591464958	25.405061164166163	26.335636563146654	23.648553681222225
22-23	24.51838752322895	25.03783206000836	26.167659210809024	24.276121205953668
24-25	24.23576586778547	25.197523530176174	26.27740546159987	24.28930514043849
26-27	24.23614681443992	25.405328326109732	26.271528991226777	24.08699586822357
28-29	24.256327407001123	25.54719994682251	26.16843340103685	24.028039245139524
30-31	24.465848792149206	25.384737179248017	26.001289309699576	24.1481247189032
32-33	24.297482688052398	25.37316534923407	25.9792783651316	24.350073597581932
34-35	24.39971525169158	25.451373005383548	26.093304615127867	24.055607127797007
36-37	24.487719129900533	25.395667349875843	25.950218637515743	24.166394882707877
38-39	24.4862175310378	25.45702102822921	25.92019960920065	24.136561831532344
40-41	24.50793259856741	25.380602403568968	25.95190249891315	24.159562498950468
42-43	24.51533680992936	25.26820134560942	25.845464019618763	24.37099782484246
44-45	24.43021467987506	25.420415066160075	25.882420301672337	24.26694995229253
46-47	24.51357165661635	25.345930293274293	25.838624543856874	24.30187350625248
48-49	24.434504646973412	25.49738921821616	25.70212716762575	24.365978967184684
50-51	24.411253827283716	25.626734609461337	25.64551436194159	24.316497201313354
52-53	24.47668104042814	25.60453883108712	25.640057943001064	24.278722185483677
54-55	24.42945476454321	25.413857656588217	25.746246168641175	24.410441410227392
56-57	24.39065922424546	25.56793596652999	25.674842437915167	24.366562371309378
58-59	24.438953196204203	25.471887570794554	25.78317570960512	24.30598352339612
60-61	24.56343004054866	25.378692584889144	25.704398512625136	24.353478861937052
62-63	24.396892000196214	25.54020604981536	25.777937185338708	24.284964764649718
64-65	24.44047506895365	25.34132639117542	25.781808922140442	24.436389617730487
66-67	24.414982697702886	25.49907960549456	25.65567262505096	24.430265071751585
68-69	24.386340356356044	25.416927471704366	25.744627913587053	24.452104258352538
70-71	24.36922014655422	25.343023332763533	25.730128693984504	24.557627826697743
72-73	24.425635748682243	25.37932633499255	25.621173723996886	24.573864192328315
74-75	24.309973427548144	25.53055459278556	25.61289937873384	24.54657260093246
76-77	24.475956518084633	25.317199294100064	25.62838345447778	24.578460733337522
78-79	24.396012421255982	25.272369563487484	25.725205335929452	24.606412679327082
80-81	24.327276271385745	25.420670713901426	25.642115094799177	24.60993791991365
82-83	24.510548575899847	25.344895105103653	25.622432799624683	24.522123519371814
84-85	24.48962100185728	25.372126509597926	25.50518055551415	24.633071933030642
86-87	24.4784733536759	25.29705162012572	25.710412123253878	24.514062902944502
88-89	24.413545610447677	25.1257210520067	25.664577482022743	24.796155855522883
90-91	24.417841692297802	25.189766678251534	25.64839704856048	24.74399458089018
92-93	24.46005816822274	25.303605475743613	25.66257287798537	24.57376347804828
94-95	24.55465915253574	25.190359847108308	25.588914420534152	24.6660665798218
96-97	24.539747312688576	25.160871575370226	25.594956018528443	24.70442509341276
98	24.591978467926804	25.120925980516933	25.433941562722563	24.853153988833704
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	559.0
1	422.5
2	465.5
3	636.0
4	543.0
5	537.0
6	846.5
7	1637.0
8	2361.5
9	2306.5
10	1783.0
11	1423.0
12	1471.0
13	1671.0
14	1869.5
15	2035.0
16	2216.0
17	2290.5
18	2238.5
19	2180.5
20	2053.5
21	1941.0
22	1859.0
23	1831.0
24	1991.0
25	2362.0
26	2932.0
27	3795.5
28	5000.5
29	6788.0
30	9420.0
31	12855.0
32	17043.0
33	21761.0
34	27219.0
35	33382.0
36	40242.5
37	47327.0
38	54785.0
39	62316.0
40	69583.0
41	77527.0
42	86330.5
43	94760.5
44	101492.5
45	108190.0
46	115476.5
47	122423.0
48	125747.0
49	125683.0
50	125664.0
51	125043.5
52	123962.5
53	123499.0
54	120892.0
55	114374.0
56	105768.0
57	97354.5
58	88822.5
59	78736.5
60	67711.5
61	56068.0
62	44985.5
63	34518.5
64	25414.0
65	19094.5
66	14398.0
67	10758.0
68	8130.0
69	6122.0
70	4615.5
71	3412.0
72	2507.0
73	1816.5
74	1324.0
75	967.5
76	637.0
77	383.5
78	234.0
79	135.0
80	77.5
81	49.5
82	36.0
83	23.0
84	11.0
85	7.5
86	9.5
87	7.5
88	6.0
89	8.0
90	6.5
91	4.0
92	3.5
93	2.5
94	4.0
95	3.5
96	3.0
97	2.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.4800243388442017
2	0.04860348461013327
3	0.05795316256567035
4	0.05509631652370069
5	0.06600427413849395
6	0.06778516517764388
7	0.04340921907927933
8	0.019478495740702265
9	0.012280727790804665
10-11	0.014655249176337894
12-13	0.011353180374580748
14-15	0.015657000385859727
16-17	0.014877860556231635
18-19	0.055856905405004306
20-21	0.08616915496720193
22-23	0.10345863880561575
24-25	0.07160666053248642
26-27	0.08795004600635185
28-29	0.08947122376895907
30-31	0.05901056662016562
32-33	0.03331750319076311
34-35	0.40084889139532814
36-37	0.6303055712208008
38-39	0.5804220711762785
40-41	0.5756359265085631
42-43	0.5485329910065002
44-45	0.5327090320857203
46-47	0.5232851503368852
48-49	0.5121360303938737
50-51	0.5268840343118341
52-53	0.6099737318571725
54-55	0.5782145083256656
56-57	0.535454572437743
58-59	0.5135459024665341
60-61	0.5261234454305305
62-63	0.5387380902911756
64-65	0.5579197708586863
66-67	0.5833345700632216
68-69	0.537161259683595
70-71	0.4847177287702948
72-73	0.46735404113858303
74-75	0.4470778546199282
76-77	0.4624194888842718
78-79	0.45629767593719395
80-81	0.4531440147220326
82-83	0.4734758540856609
84-85	0.43720875011130567
86-87	0.4418093852957763
88-89	0.4695059511442225
90-91	0.45199385592591496
92-93	0.4462616128936512
94-95	0.4576518951648808
96-97	0.4512147160962868
98	0.44622451099700217
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	2695280.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.58868402876866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.30023418628083	49.0070861686372
2	12.726330058223922	15.930485017199963
3	3.970154710822475	7.45460276222986
4	1.6883931845357383	4.226972301729353
5	0.9149640529553644	2.8633198004052427
6	0.5624038151659475	2.11200688103973
7	0.350040499618787	1.5336001959538805
8	0.25845599292471705	1.2941136381205425
9	0.19805005815389534	1.1156123260506072
>10	0.9691215533727257	10.668418164034508
>50	0.048338607019438574	2.046457873814942
>100	0.013037479114993061	1.2880829877643607
>500	2.97376132074715E-4	0.12459127685756494
>1k	1.784256792189245E-4	0.33465060616224257
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4015	0.14896411504556115	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.710189664895669E-5
2	0.0	0.0	0.0	0.0	3.710189664895669E-5
3	0.0	0.0	0.0	0.0	3.710189664895669E-5
4	0.0	0.0	0.0	0.0	3.710189664895669E-5
5	0.0	7.420379329791338E-5	0.0	0.0	3.710189664895669E-5
6	0.0	7.420379329791338E-5	0.0	0.0	3.710189664895669E-5
7	0.0	7.420379329791338E-5	0.0	0.0	3.710189664895669E-5
8	0.0	7.420379329791338E-5	0.0	0.0	3.710189664895669E-5
9	0.0	7.420379329791338E-5	0.0	0.0	3.710189664895669E-5
10-11	0.0	7.420379329791338E-5	0.0	0.0	3.710189664895669E-5
12-13	3.710189664895669E-5	1.2985663827134842E-4	0.0	3.710189664895669E-5	1.1130568994687008E-4
14-15	5.565284497343504E-5	1.4840758659582677E-4	0.0	3.710189664895669E-5	1.1130568994687008E-4
16-17	7.420379329791338E-5	1.4840758659582677E-4	0.0	7.420379329791338E-5	1.1130568994687008E-4
18-19	7.420379329791338E-5	1.4840758659582677E-4	0.0	1.6695853492030514E-4	1.4840758659582677E-4
20-21	7.420379329791338E-5	2.5971327654269685E-4	0.0	2.968151731916536E-4	1.4840758659582677E-4
22-23	7.420379329791338E-5	2.5971327654269685E-4	0.0	3.895699148140453E-4	2.0406043156926182E-4
24-25	7.420379329791338E-5	2.5971327654269685E-4	0.0	5.565284497343504E-4	2.9681517319165353E-4
26-27	7.420379329791338E-5	2.5971327654269685E-4	0.0	8.904455195749607E-4	2.9681517319165353E-4
28-29	7.420379329791338E-5	2.5971327654269685E-4	0.0	0.001966400522394705	2.9681517319165353E-4
30-31	7.420379329791338E-5	2.5971327654269685E-4	0.0	0.006455730016918465	2.9681517319165353E-4
32-33	7.420379329791338E-5	2.5971327654269685E-4	0.0	0.016324834525540947	3.7101896648956696E-4
34-35	7.420379329791338E-5	2.5971327654269685E-4	0.0	0.03018239292392627	4.0812086313852364E-4
36-37	7.420379329791338E-5	2.5971327654269685E-4	0.0	0.051126413582262324	4.0812086313852364E-4
38-39	7.420379329791338E-5	2.782642248671752E-4	0.0	0.08281143332047133	4.0812086313852364E-4
40-41	7.420379329791338E-5	2.9681517319165353E-4	0.0	0.11622169125285683	4.0812086313852364E-4
42-43	7.420379329791338E-5	3.3391706984061027E-4	0.0	0.14851889228577364	4.26671811463002E-4
44-45	9.275474162239174E-5	3.3391706984061027E-4	0.0	0.18482309815677778	4.6377370811195867E-4
46-47	1.1130568994687008E-4	3.3391706984061027E-4	0.0	0.2214612210976225	4.82324656436437E-4
48-49	1.1130568994687008E-4	3.3391706984061027E-4	0.0	0.2591010952479891	4.82324656436437E-4
50-51	1.1130568994687008E-4	3.3391706984061027E-4	0.0	0.2945890593927162	4.82324656436437E-4
52-53	1.4840758659582677E-4	3.3391706984061027E-4	0.0	0.3317466088866463	4.82324656436437E-4
54-55	1.4840758659582677E-4	3.3391706984061027E-4	0.0	0.37164969873259923	4.82324656436437E-4
56-57	1.4840758659582677E-4	3.7101896648956696E-4	0.0	0.4123690303048292	4.82324656436437E-4
58-59	1.4840758659582677E-4	3.7101896648956696E-4	0.0	0.45225356920245763	4.82324656436437E-4
60-61	1.4840758659582677E-4	3.7101896648956696E-4	0.0	0.4923978213766288	4.82324656436437E-4
62-63	1.4840758659582677E-4	3.895699148140453E-4	0.0	0.5317072808761985	4.82324656436437E-4
64-65	1.4840758659582677E-4	4.6377370811195867E-4	0.0	0.5732614051230298	4.82324656436437E-4
66-67	1.4840758659582677E-4	5.194265530853937E-4	0.0	0.6170230922204742	4.82324656436437E-4
68-69	1.4840758659582677E-4	5.194265530853937E-4	0.0	0.6615453681992223	5.194265530853937E-4
70-71	1.4840758659582677E-4	5.565284497343504E-4	0.0	0.7072363539224125	5.565284497343504E-4
72-73	1.4840758659582677E-4	5.936303463833071E-4	0.0	0.7520554450743522	6.678341396812205E-4
74-75	1.4840758659582677E-4	5.936303463833071E-4	0.0	0.7966519248463981	6.678341396812205E-4
76-77	1.4840758659582677E-4	5.936303463833071E-4	0.0	0.8445690243685258	6.678341396812205E-4
78-79	1.4840758659582677E-4	6.307322430322639E-4	0.0	0.8957881926924105	6.678341396812205E-4
80-81	1.4840758659582677E-4	6.492831913567421E-4	0.0	0.9474525837760827	6.678341396812205E-4
82-83	1.4840758659582677E-4	7.049360363301772E-4	0.0	0.998894363479861	6.863850880056989E-4
84-85	1.4840758659582677E-4	7.420379329791339E-4	0.0	1.0507999168917515	7.049360363301772E-4
86	1.4840758659582677E-4	7.420379329791339E-4	0.0	1.089794010269805	7.049360363301772E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4900	0.0	37.95854	1
GGTATCA	2870	0.0	35.130672	1
TCAACGC	5795	0.0	31.649055	4
TATCAAC	5975	0.0	30.61944	2
CAACGCA	6045	0.0	30.340162	5
ATCAACG	6145	0.0	29.697742	3
AACGCAG	6310	0.0	29.418367	6
ACGCAGA	7445	0.0	24.862242	7
GTACATG	7260	0.0	23.970629	1
CGCAGAG	7855	0.0	23.441786	8
GTACCTG	2480	0.0	22.833775	1
TACATGG	7500	0.0	22.192572	2
ACATGGG	7650	0.0	20.858356	3
TACCTGG	2660	0.0	20.685303	2
GCAGAGT	8990	0.0	20.17652	9
GAGTACA	5710	0.0	20.157104	1
AGTACAT	5455	0.0	18.912548	2
GTATATA	665	0.0	18.692455	1
CATGGGG	6000	0.0	18.11137	4
AGAGTAC	7380	0.0	17.968302	10-11
>>END_MODULE