FastQCFastQC Report
Mon 15 Aug 2016
SRR2921447_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921447_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2695280
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35450.13152622362055147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46500.041.3745571
GGTATCA27300.038.027591
TCAACGC59600.032.1970864
CAACGCA60150.031.9626375
TATCAAC60800.031.561032
ATCAACG62200.030.7748623
AACGCAG64650.030.03386
GTACATG75600.026.2045361
TACATGG76000.025.3738172
ACGCAGA76250.025.0930217
CGCAGAG81950.023.2901848
ACATGGG79550.022.7492073
GTACCTG26050.021.7542531
TACCTGG27050.020.1931442
GCAGAGT93600.019.8841089
AGAGTAC76600.019.21430610-11
CATGGGG61600.019.1228434
GAGTACG4201.8189894E-1218.1416021
GAGTACA57700.017.6621231
GAGTACT39500.017.60612312-13
Produced by FastQC (version 0.11.5)